The present study investigates the antidiabetic potential of polyphenol extracts purified from guava pulp, seeds and leaves using an in vivo experiment on albino rats. The polyphenols from guava pulp, seeds and leaves were extracted using methanol solvent and the sonication method while being evaluated by total phenolic contents and radical scavenging activity assay. The proximate composition of powders revealed that ash, protein and total sugars were significantly (p < 0.05) higher in leaves and seeds, while vitamin C was highest in pulp. Total phenolic and antioxidant activities were highest in pulp followed by leaves and seeds. The findings of feed intake and body gain revealed that the supplementation of polyphenols, especially from pulp, significantly (p < 0.05) increased the feed intake, which resulted in increased body weight. Moreover, total cholesterol (TC) and low-density lipoprotein (LDL) levels were significantly (p < 0.05) decreased, while the level of high-density lipoprotein (HDL) was increased in groups fed with polyphenols from guava pulp compared to both (+ive and –ive) control groups. Furthermore, blood glucose and triglycerides were significantly (p < 0.05) decreased in supplemented groups compared to the control group of diabetes mice, which resulted in the inhibition of α-amylase and glucose transport. Besides this, packed cell volume (PCV), mean corpuscular volume (MCV), hemoglobin, red blood cells (RBCs), white blood cells (WBCs) and platelet levels were increased significantly (p < 0.05) in pulp’s extract followed by leaves and seeds compared to both control groups. Overall, the antidiabetic potential of different extracts was in the following order: pulp > leaves > seeds. The findings suggest the feasibility of adding 200–250 mg/kg.bw of polyphenol extracts of pulp as an alternative to diabetic drugs.

Background Diosgenin is a steroidal saponin of Dioscorea and Trigonella species, which has been gaining a lot of interest due to its health-promoting action. This review will summarize the potential usefulness of diosgenin, especially with regard to the use of diosgenin in the regulation of gastrointestinal microbiota, the effects, therapeutic mechanisms, utility, and practical implications. Objectives This review aims to comprehensively examine the phytochemistry, extraction techniques, biological mechanisms, and therapeutic potential of diosgenin, with emphasis on its role in gastrointestinal health. A systematic search of peer-reviewed literature published between 2019 and 2025 was conducted via PubMed, Scopus, and Web of Science, prioritizing clinical trials, animal studies, and systematic reviews in accordance with predefined inclusion criteria. Findings Diosgenin has been shown to have the ability to defend the gut, prevent cancer by modifying the signal transduction pathway of NF-kB and MAPK; thereby reinforcing the barrier to the gut and reducing inflammation. Diosgenin is also able to shift the intestinal microbiome in favor of healthy species, including Lactobacillus acidophilus and Bifidobacterium bifidum. Bioavailability may be modulated by new approaches in nanoparticle delivery or macromolecular cyclodextrin complexes. Conclusion and future directions Diosgenin may be applied in the treatment of gastrointestinal-associated conditions such as inflammatory bowel diseases (IBD) and colorectal cancer. What also makes it clinically significant is the capability to achieve the balance of the gut-brain axis. Future clinical trials and pharmacokinetics studies need to be conducted in order to know how diosgenin is demonstrated in functional foods and personalized medicine, and be able to use them in therapies. Graphical Abstract