Heavy metals contaminate the soil that alters the properties of soil and negatively affect plants growth. Using microorganism and plant can remove these pollutants from soil. The present investigation was designed to evaluate the induced effect of Bacillus pumilus on maize plant in Cadmium (Cd) contaminated soil. Three different concentrations of Cd (i.e. 0.25, 0.50 and 0.75 mg kg⁻¹) were applied in soil under which maize plants were grown. The germination percentage, shoot length, leaf length, number of leaves, root length, fresh weight and nutrient uptake by maize plant were determined. The experiment was conducted by using complete randomized design (CRD) with three replicates. The result indicated that germination percentage, Shoot length, leaf length, root length, number of leaves, and plant fresh weight were reduced by 37, 39, 39, 32 and 59% respectively at 0.75 mg kg⁻¹ of CdSO₄ concentration but when maize seeds inoculated with Bacillus pumilus significantly increased the germination percentage, shoot length, leaf length, number of leaves, plant fresh weight at different concentrations of CdSO₄. Moreover, the plant protein were significantly increased by 60% in T6 (0.25 mg kg⁻¹ of CdSO₄ + inoculated seed) and Peroxidase dismutase (POD) was also significantly higher by 346% in T6 (0.25 mg kg⁻¹ of CdSO₄ + inoculated seed), however, the Superoxide dismutase (SOD) was significantly higher in T5 (0.75 mg kg⁻¹ of CdSO₄ + uninoculated seed) and was 769% higher as compared to control. The Cd contents in Bacillus pumilus inoculated maize roots and shoots were decreased. The present investigations indicated that the inoculation of maize plant with Bacillus pumilus can help maize plants to withstand Cd stress but higher concentration of Cd can harm the plant. The Bacillus pumilus has good potential to remediate Cd from soil, and also have potential to reduce the phyto availability and toxicity of Cd.

Purpose: Heavy metals contaminate the soil that alters the properties of soil and negatively affect plants growth. Using microorganism and plant can remove these pollutants from soil. The present investigation was designed to evaluate the induced effect of Bacillus pumilus on maize plant in Cadmium (Cd) contaminated soil. Methods: Three different concentrations of Cd (i.e. 0.25, 0.50 and 0.75 mg kg^-1) were applied in soil under which maize plants were grown. The germination percentage, shoot length, leaf length, number of leaves, root length,fresh weight and nutrient uptake by maize plant were determined. The experiment was conducted by using complete randomized design (CRD) with three replicates. Results: The result indicated that germination percentage, Shoot length, leaf length, root length, number of leaves, and plant fresh weight were reduced by 37, 39, 39, 32 and 59% respectively at 0.75mg kg^-1 of CdSO₄concentration but when maize seeds inoculated with Bacillus pumilus significantly increased the germination percentage, shoot length, leaf length, number of leaves, plant fresh weight at different concentrations of CdSO₄. Moreover, the plant protein were significantly increased by 60% in T6 (0.25 mg kg^-1 of CdSO₄+inoculated seed) and Peroxidase dismutase (POD) was also significantly higher by 346% in T6 (0.25 mg kg^-1 of CdSO₄+inoculated seed), however, the Superoxide dismutase (SOD) was significantly higher in T5 (0.75 mg kg^-1 of CdSO₄+ uninoculated seed) and was 769% higher as compared to control. The Cd contents in Bacillus pumilus inoculated maize roots and shoots were decreased. Conclusion: The present investigations indicated that the inoculation of maize plant with Bacillus pumilus can help maize plants to withstand Cd stress but higher concentration of Cd can harm the plant. The Bacillus pumilus has good potential to remediate Cd from soil, and also have potential to reduce the phytoavailibility and toxicity of Cd.

The industrial wastes comprise toxic heavy metals in huge amount which is adequate to cause noxiousness to crop plants. The zinc (Zn) and nickel (Ni) are nutrients that are crucial for plant life especially for plants growth but these elements are poisonous to plants when they are present in high concentrations. The present study was conducted to isolate endophytic bacteria from Vibernum grandiflorum and to assess the endophytic bacterial strain Bacillus mycoid (Acc MW979613) and Rock phosphate potential to overcome the stress of zinc (Zn) and nickel (Ni) toxicity on wheat plant. The concentration of nickel (Ni) (100mg/kg) and zinc (Zn) (100mg/kg) was used and eight different treatments applications were made by using Bacillus mycoid (Acc MW979613) and rock phosphate. Rock phosphate (0.2g/kg) was added in soil and experiment was conducted using complete randomized design (CRD) with three replicates. The results showed that Bacillus mycoid and application of rock phosphate (RP) significantly affected the plant growth and defense system. The treatment T12 was most prominent in which the shoot length was 57%, leaf width was 13%, protein content was 20% and sugar content was 34% higher than control respectively. In (T3) Cu content was 128%, Ca content was 219% and K content was 85% and in (T4) Mn content was 326%,Na content was 449% and K content was 84% respectively. It is concluded that Zn and Ni adversely affected the growth of wheat (Triticum aestivum) plant; however combine application of Bacillus mycoid and Rock phosphate (RP) suppressed the toxicity of zinc (Zn) and nickel (Ni) and increased plant growth, enzymes activity and nutrients uptake in wheat plants. The novel endophytic bacteria (Bacillus mycoid) and rock phosphate can be useful in combination to overcome metal stress which can improve plants growth and yield.