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Lamia Nakhle; Miriana Kfoury; Isabelle Mallard; David Landy; Hélène Greige-Gerges. Microextraction of bioactive compounds using deep eutectic solvents: a review. Environmental Chemistry Letters 2021, 1 .
AMA StyleLamia Nakhle, Miriana Kfoury, Isabelle Mallard, David Landy, Hélène Greige-Gerges. Microextraction of bioactive compounds using deep eutectic solvents: a review. Environmental Chemistry Letters. 2021; ():1.
Chicago/Turabian StyleLamia Nakhle; Miriana Kfoury; Isabelle Mallard; David Landy; Hélène Greige-Gerges. 2021. "Microextraction of bioactive compounds using deep eutectic solvents: a review." Environmental Chemistry Letters , no. : 1.
Nerolidol is a natural sesquiterpene alcohol with promising but limited application in food and pharmaceutical fields due to several factors including low photostability and low aqueous solubility. Recently, several carriers loading nerolidol were prepared and tested in fresh orange juice. Lipid vesicles loading nerolidol did not exhibit satisfactory organoleptic properties in this beverage. Hence, DMPC/DHPC bicelles were prepared as a new phospholipid-based carrier for nerolidol at different molar ratios. The bicelle suspensions were characterized in terms of homogeneity, particles size, and morphology. The optimal formulation (phospholipid:nerolidol molar ratio 100:1) was selected based on transparent appearance, homogeneity, and particle size (~ 45 nm). Besides, it showed a high encapsulation efficiency of nerolidol and a high incorporation rate of phospholipids. Transmission electron microscopy analysis demonstrated the formation of bicelles. The bicelles membrane fluidity was assessed by 1,6-diphenyl-1,3,5-hexatriene fluorescence anisotropy and differential scanning calorimetry analysis. The membrane fluidity of bicelles appeared to increase in the presence of nerolidol in a concentration dependent manner. To our knowledge this is the first study dealing with the encapsulation of an essential oil component in bicelles.
Elissa Ephrem; Amal Najjar; Catherine Charcosset; Hélène Greige-Gerges. Bicelles as a carrier for bioactive compounds in beverages: application to nerolidol, an active sesquiterpene alcohol. Journal of Food Science and Technology 2021, 1 -10.
AMA StyleElissa Ephrem, Amal Najjar, Catherine Charcosset, Hélène Greige-Gerges. Bicelles as a carrier for bioactive compounds in beverages: application to nerolidol, an active sesquiterpene alcohol. Journal of Food Science and Technology. 2021; ():1-10.
Chicago/Turabian StyleElissa Ephrem; Amal Najjar; Catherine Charcosset; Hélène Greige-Gerges. 2021. "Bicelles as a carrier for bioactive compounds in beverages: application to nerolidol, an active sesquiterpene alcohol." Journal of Food Science and Technology , no. : 1-10.
Deep eutectic solvents have emerged in green chemistry only seventeen years ago and yet resulted in a plethora of publications covering various research areas and diverse fields of application. Deep eutectic solvents appear as promising alternatives to conventional organic solvents due to their straightforward preparation using highly accessible and natural compounds. They display also high tunability. Here we present the classification and preparation methods of deep eutectic solvents. We detail their physicochemical properties such as phase behavior, density, viscosity, ionic conductivity, surface tension, and polarity. Properties are controlled by the choice of the forming compounds, molar ratio, temperature, and water content.
Tracy El Achkar; Hélène Greige-Gerges; Sophie Fourmentin. Basics and properties of deep eutectic solvents: a review. Environmental Chemistry Letters 2021, 19, 3397 -3408.
AMA StyleTracy El Achkar, Hélène Greige-Gerges, Sophie Fourmentin. Basics and properties of deep eutectic solvents: a review. Environmental Chemistry Letters. 2021; 19 (4):3397-3408.
Chicago/Turabian StyleTracy El Achkar; Hélène Greige-Gerges; Sophie Fourmentin. 2021. "Basics and properties of deep eutectic solvents: a review." Environmental Chemistry Letters 19, no. 4: 3397-3408.
In recent years, liposomes have gained increasing attention for their potential applications as drug delivery systems in the pharmaceutic, cosmetic and food industries. However, they have a tendency to aggregate and are sensitive to degradation caused by several factors, which may limit their effectiveness. A promising approach to improve liposomal stability is to modify liposomal surfaces by forming polymeric layers. Among natural polymers, chitosan has received great interest due to its biocompatibility and biodegradability. This review discussed the characteristics of this combined system, called chitosomes, in comparison to those of conventional liposomes. The coating of liposomes with chitosan or its derivatives improved liposome stability, provided sustained drug release and increased drug penetration across mucus layers. The mechanisms behind these results are highlighted in this paper. Alternative assembly of polyelectrolytes using alginate, sodium hyaluronate, or pectin with chitosan could further improve the liposomal characteristics. Chitosomal encapsulation could also ensure targeted delivery and boost the antimicrobial efficacy of essential oils (EOs). Moreover, chitosomes could be an efficient tool to overcome the major drawbacks related to the chemical properties of EOs (low water solubility, sensitivity to oxygen, light, heat, and humidity) and their poor bioavailability. Overall, chitosomes could be considered as a promising strategy to enlarge the use of liposomes.
Carine Sebaaly; Adriana Trifan; Elwira Sieniawska; Hélène Greige-Gerges. Chitosan-Coating Effect on the Characteristics of Liposomes: A Focus on Bioactive Compounds and Essential Oils: A Review. Processes 2021, 9, 445 .
AMA StyleCarine Sebaaly, Adriana Trifan, Elwira Sieniawska, Hélène Greige-Gerges. Chitosan-Coating Effect on the Characteristics of Liposomes: A Focus on Bioactive Compounds and Essential Oils: A Review. Processes. 2021; 9 (3):445.
Chicago/Turabian StyleCarine Sebaaly; Adriana Trifan; Elwira Sieniawska; Hélène Greige-Gerges. 2021. "Chitosan-Coating Effect on the Characteristics of Liposomes: A Focus on Bioactive Compounds and Essential Oils: A Review." Processes 9, no. 3: 445.
The Mediterranean diet is associated with health benefits due to bioactive compounds such as polyphenols. The biological activities of three polyphenols (quercetin (QCT), resveratrol (RSV), apigenin (API)) were evaluated in mouse neuronal N2a cells in the presence of 7-ketocholesterol (7KC), a major cholesterol oxidation product increased in patients with age-related diseases, including neurodegenerative disorders. In N2a cells, 7KC (50 µM; 48 h) induces cytotoxic effects characterized by an induction of cell death. When associated with RSV, QCT and API (3.125; 6.25 µM), 7KC-induced toxicity was reduced. The ability of QCT, RSV and API to prevent 7KC-induced oxidative stress was characterized by a decrease in reactive oxygen species (ROS) production in whole cells and at the mitochondrial level; by an attenuation of the increase in the level and activity of catalase; by attenuating the decrease in the expression, level and activity of glutathione peroxidase 1 (GPx1); by normalizing the expression, level and activity of superoxide dismutases 1 and 2 (SOD1, SOD2); and by reducing the decrease in the expression of nuclear erythroid 2-like factor 2 (Nrf2) which regulates antioxidant genes. QCT, RSV and API also prevented mitochondrial dysfunction in 7KC-treated cells by counteracting the loss of mitochondrial membrane potential (ΨΔm) and attenuating the decreased gene expression and/or protein level of AMP-activated protein kinase α (AMPKα), sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) implicated in mitochondrial biogenesis. At the peroxisomal level, QCT, RSV and API prevented the impact of 7KC by counteracting the decrease in ATP binding cassette subfamily D member (ABCD)3 (a peroxisomal mass marker) at the protein and mRNA levels, as well as the decreased expresssion of genes associated with peroxisomal biogenesis (Pex13, Pex14) and peroxisomal β-oxidation (Abcd1, Acox1, Mfp2, Thiolase A). The 7KC-induced decrease in ABCD1 and multifunctional enzyme type 2 (MFP2), two proteins involved in peroxisomal β-oxidation, was also attenuated by RSV, QCT and API. 7KC-induced cell death, which has characteristics of apoptosis (cells with fragmented and/or condensed nuclei; cleaved caspase-3; Poly(ADP-ribose) polymerase (PARP) fragmentation) and autophagy (cells with monodansyl cadaverine positive vacuoles; activation of microtubule associated protein 1 light chain 3–I (LC3-I) to LC3-II, was also strongly attenuated by RSV, QCT and API. Thus, in N2a cells, 7KC induces a mode of cell death by oxiapoptophagy, including criteria of OXIdative stress, APOPTOsis and autoPHAGY, associated with mitochondrial and peroxisomal dysfunction, which is counteracted by RSV, QCT, and API reinforcing the interest for these polyphenols in prevention of diseases associated with increased 7KC levels.
Aline Yammine; Amira Zarrouk; Thomas Nury; Anne Vejux; Norbert Latruffe; Dominique Vervandier-Fasseur; Mohammad Samadi; John J. Mackrill; Hélène Greige-Gerges; Lizette Auezova; Gérard Lizard. Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases. Cells 2020, 9, 2346 .
AMA StyleAline Yammine, Amira Zarrouk, Thomas Nury, Anne Vejux, Norbert Latruffe, Dominique Vervandier-Fasseur, Mohammad Samadi, John J. Mackrill, Hélène Greige-Gerges, Lizette Auezova, Gérard Lizard. Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases. Cells. 2020; 9 (11):2346.
Chicago/Turabian StyleAline Yammine; Amira Zarrouk; Thomas Nury; Anne Vejux; Norbert Latruffe; Dominique Vervandier-Fasseur; Mohammad Samadi; John J. Mackrill; Hélène Greige-Gerges; Lizette Auezova; Gérard Lizard. 2020. "Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases." Cells 9, no. 11: 2346.
Nowadays, scientists are going through developing more eco-friendly analysis methods of simple and complex samples that follow the principles of “green chemistry.” One of the followed strategies is by replacing the toxic conventional organic solvents used in the extraction by a new generation of solvents called “deep eutectic solvent” or “natural deep eutectic solvent.” These solvents are formed between two or more cheap nontoxic components via hydrogen bonds. This review presents the various extraction methods that use deep eutectic solvents as extraction solvents, mainly the liquid-liquid-phase microextraction methods, solid-phase microextraction methods, and the newly combined techniques. In addition, the advantages and drawbacks of using these green solvents in comparison to the conventional organic solvents used in conventional extraction methods are discussed. It was observed that, with all reported extractions, deep eutectic solvents showed better extraction efficiency and higher recovery values for the studied natural target analytes compared to water and lots of conventional organic solvents. For the protein extraction, these solvents showed around 93–99% extraction efficiency. In addition, new types of deep eutectic solvents, like the ternary deep eutectic solvent molecularly imprinted polymer, were synthesized, improving the solvent characteristics; therefore, lower volume of the solvent was used with shorter extraction time.
Lamia Nakhle; Miriana Kfoury; Isabelle Mallard; David Landy; Hélène Greige-Gerges. Methods for Extraction of Bioactive Compounds from Plant and Animal Matter Using Deep Eutectic Solvents. Nanosensors for Environment, Food and Agriculture Vol. 1 2020, 183 -240.
AMA StyleLamia Nakhle, Miriana Kfoury, Isabelle Mallard, David Landy, Hélène Greige-Gerges. Methods for Extraction of Bioactive Compounds from Plant and Animal Matter Using Deep Eutectic Solvents. Nanosensors for Environment, Food and Agriculture Vol. 1. 2020; ():183-240.
Chicago/Turabian StyleLamia Nakhle; Miriana Kfoury; Isabelle Mallard; David Landy; Hélène Greige-Gerges. 2020. "Methods for Extraction of Bioactive Compounds from Plant and Animal Matter Using Deep Eutectic Solvents." Nanosensors for Environment, Food and Agriculture Vol. 1 , no. : 183-240.
The brain, which is a cholesterol-rich organ, can be subject to oxidative stress in a variety of pathophysiological conditions, age-related diseases and some rare pathologies. This can lead to the formation of 7-ketocholesterol (7KC), a toxic derivative of cholesterol mainly produced by auto-oxidation. So, preventing the neuronal toxicity of 7KC is an important issue to avoid brain damage. As there are numerous data in favor of the prevention of neurodegeneration by the Mediterranean diet, this study aimed to evaluate the potential of a series of polyphenols (resveratrol, RSV; quercetin, QCT; and apigenin, API) as well as ω3 and ω9 unsaturated fatty acids (α-linolenic acid, ALA; eicosapentaenoic acid, EPA; docosahexaenoic acid, DHA, and oleic acid, OA) widely present in this diet, to prevent 7KC (50 µM)-induced dysfunction of N2a neuronal cells. When polyphenols and fatty acids were used at non-toxic concentrations (polyphenols: ≤6.25 µM; fatty acids: ≤25 µM) as defined by the fluorescein diacetate assay, they greatly reduce 7KC-induced toxicity. The cytoprotective effects observed with polyphenols and fatty acids were comparable to those of α-tocopherol (400 µM) used as a reference. These polyphenols and fatty acids attenuate the overproduction of reactive oxygen species and the 7KC-induced drop in mitochondrial transmembrane potential (ΔΨm) measured by flow cytometry after dihydroethidium and DiOC6(3) staining, respectively. Moreover, the studied polyphenols and fatty acids reduced plasma membrane permeability considered as a criterion for cell death measured by flow cytometry after propidium iodide staining. Our data show that polyphenols (RSV, QCT and API) as well as ω3 and ω9 unsaturated fatty acids (ALA, EPA, DHA and OA) are potent cytoprotective agents against 7KC-induced neurotoxicity in N2a cells. Their cytoprotective effects could partly explain the benefits of the Mediterranean diet on human health, particularly in the prevention of neurodegenerative diseases.
Aline Yammine; Thomas Nury; Anne Vejux; Norbert Latruffe; Dominique Vervandier-Fasseur; Mohammad Samadi; Hélène Greige-Gerges; Lizette Auezova; Gérard Lizard. Prevention of 7-Ketocholesterol-Induced Overproduction of Reactive Oxygen Species, Mitochondrial Dysfunction and Cell Death with Major Nutrients (Polyphenols, ω3 and ω9 Unsaturated Fatty Acids) of the Mediterranean Diet on N2a Neuronal Cells. Molecules 2020, 25, 2296 .
AMA StyleAline Yammine, Thomas Nury, Anne Vejux, Norbert Latruffe, Dominique Vervandier-Fasseur, Mohammad Samadi, Hélène Greige-Gerges, Lizette Auezova, Gérard Lizard. Prevention of 7-Ketocholesterol-Induced Overproduction of Reactive Oxygen Species, Mitochondrial Dysfunction and Cell Death with Major Nutrients (Polyphenols, ω3 and ω9 Unsaturated Fatty Acids) of the Mediterranean Diet on N2a Neuronal Cells. Molecules. 2020; 25 (10):2296.
Chicago/Turabian StyleAline Yammine; Thomas Nury; Anne Vejux; Norbert Latruffe; Dominique Vervandier-Fasseur; Mohammad Samadi; Hélène Greige-Gerges; Lizette Auezova; Gérard Lizard. 2020. "Prevention of 7-Ketocholesterol-Induced Overproduction of Reactive Oxygen Species, Mitochondrial Dysfunction and Cell Death with Major Nutrients (Polyphenols, ω3 and ω9 Unsaturated Fatty Acids) of the Mediterranean Diet on N2a Neuronal Cells." Molecules 25, no. 10: 2296.
The aminothiol cysteamine, deriving from coenzyme A degradation in mammalian cells, presents several biological applications. However, the bitter taste and sickening odor, the chemical instability, the hygroscopicity, and the poor pharmacokinetic profile of cysteamine limit its efficacy. The use of encapsulation systems is a good methodology to overcome these undesirable properties and improve the pharmacokinetic behavior of cysteamine. Besides, the conjugation of cysteamine to the surface of nanoparticles is generally proposed to improve the intra-oral delivery of cyclodextrin-drug inclusion complexes, as well as to enhance the colorimetric detection of compounds by a gold nanoparticle aggregation method. On the other hand, the detection and quantification of cysteamine is a challenging mission due to the lack of a chromophore in its structure and its susceptibility to oxidation before or during the analysis. Derivatization agents are therefore applied for the quantification of this molecule. To our knowledge, the derivatization techniques and the encapsulation systems used for cysteamine delivery were not reviewed previously. Thus, this review aims to compile all the data on these methods as well as to provide an overview of the various biological applications of cysteamine focusing on its skin application.
Carla Atallah; Catherine Charcosset; Hélène Greige-Gerges. Challenges for cysteamine stabilization, quantification, and biological effects improvement. Journal of Pharmaceutical Analysis 2020, 10, 499 -516.
AMA StyleCarla Atallah, Catherine Charcosset, Hélène Greige-Gerges. Challenges for cysteamine stabilization, quantification, and biological effects improvement. Journal of Pharmaceutical Analysis. 2020; 10 (6):499-516.
Chicago/Turabian StyleCarla Atallah; Catherine Charcosset; Hélène Greige-Gerges. 2020. "Challenges for cysteamine stabilization, quantification, and biological effects improvement." Journal of Pharmaceutical Analysis 10, no. 6: 499-516.
Eucalyptol (Euc) is a natural monoterpene with insecticide effects. Being highly volatile and sensitive to ambient conditions, its encapsulation would enlarge its application. Euc‐loaded conventional liposomes (CL), cyclodextrin/drug inclusion complex and drug‐in‐cyclodextrin‐in‐liposomes (DCL) were prepared to protect Euc from degradation, reduce its evaporation and provide its controlled release. The liposomal suspension was freeze‐dried using hydroxypropyl‐ß‐cyclodextrin (HP‐ß‐CD) as cryoprotectant. The liposomes were characterized before and after freeze‐drying. The effect of Euc on the fluidity of liposomal membrane was also examined. A release study of Euc from delivery systems, in powder and reconstituted forms, was performed by multiple head extraction at 60 °C after 6 months of storage at 4 °C. CL and DCL suspensions were homogeneous, showed nanometric vesicles size, spherical shape and negative surface charge before and after freeze‐drying. Moreover, HP‐ß‐CD did not affect the fluidity of liposomes. CL formulations presented a weak encapsulation for Euc. The loading capacity of eucalyptol in DCL was 38 times higher than that in CL formulation. In addition, freeze‐dried DCL and HP‐ß‐CD/Euc inclusion complex showed a higher retention of eucalyptol than CL delivery system. Both carrier systems HP‐ß‐CD/Euc and Euc‐loaded DCL decreased Euc evaporation and improved its retention. Practical Applications: Eucalyptol is an] natural insecticide. It is highly volatile and poorly soluble in water. To enlarge its application, we studied its encapsulation in three delivery systems (conventional liposomes, cyclodextrin/drug inclusion complex, combined system composed of cyclodextrin inclusion complex and liposome). In this paper we proved that cyclodextrin/eucalyptol inclusion complex and eucalyptol‐in‐cyclodextrin‐in‐liposome are effective delivery systems for encalyptol encapsulation, retention and release. This article is protected by copyright. All rights reserved
Riham Gharib; Jouda Mediouni Ben Jemâa; Catherine Charcosset; Sophie Fourmentin; Hélène Greige‐Gerges. Retention of Eucalyptol, a Natural Volatile Insecticide, in Delivery Systems Based on Hydroxypropyl‐β‐Cyclodextrin and Liposomes. European Journal of Lipid Science and Technology 2020, 122, 1 .
AMA StyleRiham Gharib, Jouda Mediouni Ben Jemâa, Catherine Charcosset, Sophie Fourmentin, Hélène Greige‐Gerges. Retention of Eucalyptol, a Natural Volatile Insecticide, in Delivery Systems Based on Hydroxypropyl‐β‐Cyclodextrin and Liposomes. European Journal of Lipid Science and Technology. 2020; 122 (5):1.
Chicago/Turabian StyleRiham Gharib; Jouda Mediouni Ben Jemâa; Catherine Charcosset; Sophie Fourmentin; Hélène Greige‐Gerges. 2020. "Retention of Eucalyptol, a Natural Volatile Insecticide, in Delivery Systems Based on Hydroxypropyl‐β‐Cyclodextrin and Liposomes." European Journal of Lipid Science and Technology 122, no. 5: 1.
Liposomes are lipid vesicles made of one or multiple lipid bilayers surrounding an internal aqueous core. They are broadly employed as models to study membrane structure and properties. Among these properties, liposome membrane permeability is crucial and widely assessed by fluorescence techniques. The first part of this review is devoted to describe the various techniques used for membrane permeability assessment. Attention is paid to fluorescence techniques based on vesicle leakage of self-quenching probes, dye/quencher pair or cation/ligand pair. Secondly, the membrane-active agents inducing membrane permeabilization is presented and details on their mechanisms of action are given. Emphasis is also laid on the intrinsic and extrinsic factors that can modulate the membrane permeability. Hence, a suitable liposomal membrane should be formulated according to the aim of the study and its application.
Ghenwa Nasr; Hélène Greige-Gerges; Abdelhamid Elaissari; Nathalie Khreich. Liposomal membrane permeability assessment by fluorescence techniques: Main permeabilizing agents, applications and challenges. International Journal of Pharmaceutics 2020, 580, 119198 .
AMA StyleGhenwa Nasr, Hélène Greige-Gerges, Abdelhamid Elaissari, Nathalie Khreich. Liposomal membrane permeability assessment by fluorescence techniques: Main permeabilizing agents, applications and challenges. International Journal of Pharmaceutics. 2020; 580 ():119198.
Chicago/Turabian StyleGhenwa Nasr; Hélène Greige-Gerges; Abdelhamid Elaissari; Nathalie Khreich. 2020. "Liposomal membrane permeability assessment by fluorescence techniques: Main permeabilizing agents, applications and challenges." International Journal of Pharmaceutics 580, no. : 119198.
A new deep eutectic solvent (DES), based on a methylated-β-cyclodextrin and levulinic acid, exhibiting supramolecular properties is presented here for the first time.
Tracy El Achkar; Tarek Moufawad; Steven Ruellan; David Landy; Hélène Greige-Gerges; Sophie Fourmentin. Cyclodextrins: from solute to solvent. Chemical Communications 2020, 56, 3385 -3388.
AMA StyleTracy El Achkar, Tarek Moufawad, Steven Ruellan, David Landy, Hélène Greige-Gerges, Sophie Fourmentin. Cyclodextrins: from solute to solvent. Chemical Communications. 2020; 56 (23):3385-3388.
Chicago/Turabian StyleTracy El Achkar; Tarek Moufawad; Steven Ruellan; David Landy; Hélène Greige-Gerges; Sophie Fourmentin. 2020. "Cyclodextrins: from solute to solvent." Chemical Communications 56, no. 23: 3385-3388.
Antibacterial activities of phenylpropenes (PPs) (eugenol, isoeugenol, estragole and trans-anethole) and hydroxycinnamic acids (HCAs) (p-coumaric, caffeic and ferulic acids) were assessed against Escherichia coli and Staphylococcus epidermidis. Effect of cyclodextrin and liposome encapsulation on the PPs activity was also evaluated. All PPs inhibited the bacterial growth in the hundred micromolar range, while HCAs did not, as determined by broth macrodilution. Anethole and estragole showed a higher efficiency than eugenol and isoeugenol, and E. coli was more susceptible than S. epidermidis. Hydroxypropyl-β-cyclodextrin/PP complexes and anethole-loaded Lipoid S100-liposomes were prepared by freeze-drying and ethanol injection respectively. Both formulations were substantially less active than free PPs. For instance, E. coli growth inhibition was about 14% for all HP-β-CD/PP complexes evaluated at MIC50 values of free PPs (P < 0·05), and about 12% for liposomal anethole evaluated at minimal bactericidal concentration value of free anethole (P < 0·05). Hydrophobicity appears to be crucial for PPs antibacterial activity. Encapsulation in cyclodextrin and liposome seems to retain the PPs preventing their interaction with bacteria. This study highlights the structural features of simple phenylpropanoids related to their antibacterial activity. The limitations of conventional encapsulation systems on the activity of PPs should be considered in future applications.
L. Auezova; A. Najjar; M. Kfoury; S. Fourmentin; H. Greige‐Gerges. Antibacterial activity of free or encapsulated selected phenylpropanoids against Escherichia coli and Staphylococcus epidermidis. Journal of Applied Microbiology 2019, 128, 710 -720.
AMA StyleL. Auezova, A. Najjar, M. Kfoury, S. Fourmentin, H. Greige‐Gerges. Antibacterial activity of free or encapsulated selected phenylpropanoids against Escherichia coli and Staphylococcus epidermidis. Journal of Applied Microbiology. 2019; 128 (3):710-720.
Chicago/Turabian StyleL. Auezova; A. Najjar; M. Kfoury; S. Fourmentin; H. Greige‐Gerges. 2019. "Antibacterial activity of free or encapsulated selected phenylpropanoids against Escherichia coli and Staphylococcus epidermidis." Journal of Applied Microbiology 128, no. 3: 710-720.
Lactobacillus fermentum is commonly responsible for fruit juice fermentation and spoilage. The aim of this study was to investigate the potential use of nerolidol to control the spoilage of fresh orange juice by L. fermentum. Nerolidol was incorporated into hydroxypropyl-β-cyclodextrin inclusion complex, conventional liposome, and drug-in-cyclodextrin-in liposome systems. The systems were lyophilized and characterized with respect to their nerolidol content, size, and morphology. The effects of the acidity and cold storage of orange juice on the survival of L. fermentum were evaluated. Subsequently, the antibacterial activity of nerolidol in refrigerated orange juice was assessed at pH 3.3. Nerolidol showed a faster antibacterial activity at 4 000 μM (5 days) compared to 2 000 μM (8 days). Under the same conditions, the inclusion complex completely killed bacteria within 6 days of incubation at 4 000 μM, suggesting its potential application in fruit juices. Nerolidol-loaded liposomes did not exhibit an antibacterial activity and altered the appearance of juice.
Elissa Ephrem; Amal Najjar; Catherine Charcosset; Hélène Greige-Gerges. Use of free and encapsulated nerolidol to inhibit the survival of Lactobacillus fermentum in fresh orange juice. Food and Chemical Toxicology 2019, 133, 110795 .
AMA StyleElissa Ephrem, Amal Najjar, Catherine Charcosset, Hélène Greige-Gerges. Use of free and encapsulated nerolidol to inhibit the survival of Lactobacillus fermentum in fresh orange juice. Food and Chemical Toxicology. 2019; 133 ():110795.
Chicago/Turabian StyleElissa Ephrem; Amal Najjar; Catherine Charcosset; Hélène Greige-Gerges. 2019. "Use of free and encapsulated nerolidol to inhibit the survival of Lactobacillus fermentum in fresh orange juice." Food and Chemical Toxicology 133, no. : 110795.
The search for green solvents that can replace harsh organic solvents and relatively toxic ionic liquids (ILs) has led to the discovery of deep eutectic solvents (DES). DES are now rapidly emerging in numerous applications owing to their green character, biodegradability, tuneability, low cost and simple preparation, compared to other solvents. The possibility that natural DES might play a role as an alternative media to water in living organisms pushed the researchers to investigate their use as solvents for the poorly water soluble macromolecules. However, the addition of water in most of their applications must be controlled to maintain DES' supramolecular structure and properties. This review presents the impact of water on DES, used as solvents for the biological macromolecules mainly phospholipids, proteins, nucleic acids and polysaccharides. The effect of water on the DES' supramolecular network and its physico-chemical properties is discussed and the applications of DES as solvents for the biological macromolecules, in the absence and presence of water, are highlighted.
Tracy El Achkar; Sophie Fourmentin; Hélène Greige-Gerges. Deep eutectic solvents: An overview on their interactions with water and biochemical compounds. Journal of Molecular Liquids 2019, 288, 111028 .
AMA StyleTracy El Achkar, Sophie Fourmentin, Hélène Greige-Gerges. Deep eutectic solvents: An overview on their interactions with water and biochemical compounds. Journal of Molecular Liquids. 2019; 288 ():111028.
Chicago/Turabian StyleTracy El Achkar; Sophie Fourmentin; Hélène Greige-Gerges. 2019. "Deep eutectic solvents: An overview on their interactions with water and biochemical compounds." Journal of Molecular Liquids 288, no. : 111028.
Cyclodextrins (CDs) are cyclic oligosaccharides able to improve drug water solubility and stability by forming CD/drug inclusion complexes. To further increase drug entrapment and delay its release, the CD/drug inclusion complex can be embedded in the aqueous phase of a liposome, a lipid vesicle composed of phospholipid bilayer surrounding an aqueous compartment. The resulting carrier is known as drug-in-cyclodextrin-in-liposome (DCL) system. CDs and DCLs are recognized as effective drug delivery systems; therefore, understanding the interaction of CDs with liposomal and biological membranes is of great importance. CDs are able to extract phospholipids, cholesterol, and proteins from membranes; the effect depends on the membrane structure and composition as well as on the CD type and concentration. Under definite conditions, CDs can affect the membrane fluidity, permeability, and stability of liposomes and cells, leading to the leakage of some of their internal constituents. On the other side, CDs demonstrated their beneficial effects on the membrane structure, including preservation of the membrane integrity during freeze-drying. In this paper, we review the literature concerning the interaction of CDs with biomimetic and biological membranes. Moreover, the impact of CDs on the membrane properties, mainly fluidity, stability, and permeability, is highlighted.
Zahraa Hammoud; Nathalie Khreich; Lizette Auezova; Sophie Fourmentin; Abdelhamid Elaissari; Hélène Greige-Gerges. Cyclodextrin-membrane interaction in drug delivery and membrane structure maintenance. International Journal of Pharmaceutics 2019, 564, 59 -76.
AMA StyleZahraa Hammoud, Nathalie Khreich, Lizette Auezova, Sophie Fourmentin, Abdelhamid Elaissari, Hélène Greige-Gerges. Cyclodextrin-membrane interaction in drug delivery and membrane structure maintenance. International Journal of Pharmaceutics. 2019; 564 ():59-76.
Chicago/Turabian StyleZahraa Hammoud; Nathalie Khreich; Lizette Auezova; Sophie Fourmentin; Abdelhamid Elaissari; Hélène Greige-Gerges. 2019. "Cyclodextrin-membrane interaction in drug delivery and membrane structure maintenance." International Journal of Pharmaceutics 564, no. : 59-76.
The encapsulation of essential oil components into liposomes was demonstrated to improve their solubility and chemical stability. In this study, we investigated the effect of chemical structure, Henry’s law constant (Hc), and aqueous solubility of essential oil components on their liposomal encapsulation. Estragole, eucalyptol, isoeugenol, pulegone, terpineol, and thymol were encapsulated in lipoid S100-liposomes using the ethanol injection method. The Hc values were determined. The incorporation in liposomes was more efficient (encapsulation efficiency > 90 %) for the essential oil components exhibiting low aqueous solubility (estragole, isoeugenol, and pulegone). Moreover, efficient entrapment into vesicles (loading rate > 18 %) was obtained for isoeugenol, terpineol, and thymol. This result suggests that the presence of a hydroxyl group in the structure and a low Hc value enhance the entrapment of essential oil components into liposomes. Furthermore, drug release rate from liposomes was controlled by the loading rate of essential oil components into liposomes, the size of particles, the location of essential oil components within the lipid bilayer, and the cholesterol incorporation rate of liposomes. Finally, considerable concentrations of isoeugenol, pulegone, terpineol, and thymol were retained in liposomes after 10 months with respect to the initial concentration.
Zahraa Hammoud; Riham Gharib; Sophie Fourmentin; Abdelhamid Elaissari; Hélène Greige-Gerges. New findings on the incorporation of essential oil components into liposomes composed of lipoid S100 and cholesterol. International Journal of Pharmaceutics 2019, 561, 161 -170.
AMA StyleZahraa Hammoud, Riham Gharib, Sophie Fourmentin, Abdelhamid Elaissari, Hélène Greige-Gerges. New findings on the incorporation of essential oil components into liposomes composed of lipoid S100 and cholesterol. International Journal of Pharmaceutics. 2019; 561 ():161-170.
Chicago/Turabian StyleZahraa Hammoud; Riham Gharib; Sophie Fourmentin; Abdelhamid Elaissari; Hélène Greige-Gerges. 2019. "New findings on the incorporation of essential oil components into liposomes composed of lipoid S100 and cholesterol." International Journal of Pharmaceutics 561, no. : 161-170.
Essential oil components are widely used for their antibacterial activity against spoilage microorganisms in food. Lactobacillus fermentum is a Gram-positive lactic acid bacteria responsible for the deterioration of various food products, including beverages and dairy products. In this study, 17 terpenic and 11 phenolic compounds were screened against the food spoilage microorganism Lactobacillus fermentum ATCC 9338. The antibacterial activity of the tested compounds was dependent on hydrophobicity and particular chemical features. Nerolidol solubilized in dimethylsulfoxide exhibited the highest antibacterial activity and showed low minimal inhibitory (MIC: 25 μM) and minimal bactericidal (MBC: 50 μM) concentrations. Moreover, no viable cells were detected within 16 h of incubation at 50 μM. The important antibacterial activity of nerolidol against L. fermentum is probably related to the high hydrophobicity, the aliphatic chain length, and the presence of the hydroxyl group. Hydroxypropyl-β-cyclodextrin/nerolidol inclusion complex showed MIC and MBC values of 100 and 200 μM, respectively. The total bacterial kill was observed after 12 h of incubation. The results obtained with the inclusion complex are probably due to the time required to allow nerolidol to be released from the inclusion complex.
Elissa Ephrem; Amal Najjar; Catherine Charcosset; Hélène Greige-Gerges. Selection of nerolidol among a series of terpenic and phenolic compounds for its potent activity against Lactobacillus fermentum ATCC 9338. Process Biochemistry 2019, 80, 146 -156.
AMA StyleElissa Ephrem, Amal Najjar, Catherine Charcosset, Hélène Greige-Gerges. Selection of nerolidol among a series of terpenic and phenolic compounds for its potent activity against Lactobacillus fermentum ATCC 9338. Process Biochemistry. 2019; 80 ():146-156.
Chicago/Turabian StyleElissa Ephrem; Amal Najjar; Catherine Charcosset; Hélène Greige-Gerges. 2019. "Selection of nerolidol among a series of terpenic and phenolic compounds for its potent activity against Lactobacillus fermentum ATCC 9338." Process Biochemistry 80, no. : 146-156.
Muhammad B. Asif; Matteo Bianchi; Cristiana Boi; Catherine Charcosset; Marco Cocchi; Peter Czermak; Robert H. Davis; Rong Fan; Hélène Greige-Gerges; Faisal I. Hai; Zisu Hao; Daniel Hoffmann; Veeriah Jegatheesan; Ulrich Kulozik; Jasmin Leber; A. Livingston; Daniel Loewe; Keven Lothert; Leone Mazzeo; Namila; Long D. Nghiem; Tobias Oppermann; L. Peeva; Vincenzo Piemonte; William E. Price; Xianghong Qian; S. Ranil Wickramasighe; Denise Salzig; Carine Sebaaly; Goran T. Vladisavljević; Tobias Weidner; W.S. Winston Ho; Michael Wolff; Kazuo Yamamoto; Jan Zitzmann. Contributors. Current Trends and Future Developments on (Bio-) Membranes 2019, 1 .
AMA StyleMuhammad B. Asif, Matteo Bianchi, Cristiana Boi, Catherine Charcosset, Marco Cocchi, Peter Czermak, Robert H. Davis, Rong Fan, Hélène Greige-Gerges, Faisal I. Hai, Zisu Hao, Daniel Hoffmann, Veeriah Jegatheesan, Ulrich Kulozik, Jasmin Leber, A. Livingston, Daniel Loewe, Keven Lothert, Leone Mazzeo, Namila, Long D. Nghiem, Tobias Oppermann, L. Peeva, Vincenzo Piemonte, William E. Price, Xianghong Qian, S. Ranil Wickramasighe, Denise Salzig, Carine Sebaaly, Goran T. Vladisavljević, Tobias Weidner, W.S. Winston Ho, Michael Wolff, Kazuo Yamamoto, Jan Zitzmann. Contributors. Current Trends and Future Developments on (Bio-) Membranes. 2019; ():1.
Chicago/Turabian StyleMuhammad B. Asif; Matteo Bianchi; Cristiana Boi; Catherine Charcosset; Marco Cocchi; Peter Czermak; Robert H. Davis; Rong Fan; Hélène Greige-Gerges; Faisal I. Hai; Zisu Hao; Daniel Hoffmann; Veeriah Jegatheesan; Ulrich Kulozik; Jasmin Leber; A. Livingston; Daniel Loewe; Keven Lothert; Leone Mazzeo; Namila; Long D. Nghiem; Tobias Oppermann; L. Peeva; Vincenzo Piemonte; William E. Price; Xianghong Qian; S. Ranil Wickramasighe; Denise Salzig; Carine Sebaaly; Goran T. Vladisavljević; Tobias Weidner; W.S. Winston Ho; Michael Wolff; Kazuo Yamamoto; Jan Zitzmann. 2019. "Contributors." Current Trends and Future Developments on (Bio-) Membranes , no. : 1.
Many novel bacterial targets and natural inhibitors of enzymes are currently being considered to overcome antibiotic resistance of Escherichia coli. Hence, in this study, 20 essential oil constituents were screened for their potential inhibitory effect on E. coli ATP synthase. This enzyme is involved in the hydrolysis of ATP into ADP and inorganic phosphate (Pi). First, E. coli membrane ATP synthase was isolated via cell lysis. A spectrophotometric method was optimized to quantify the released phosphate from ATP hydrolysis in order to follow the enzymatic activity. The method was validated by determining the kinetic parameters of this reaction (Km = 144.66 μM and Vmax = 270.27 μM/min), and through the inhibition assays of ATP synthase using three reference inhibitors, thymoquinone (half maximal inhibitory concentration [IC50] = 50.93 μM), resveratrol (maximum inhibition of 40%), and quercetin (IC50 = 29.01 μM). Among the studied essential oil components, α‐terpinene was the most potent inhibitor (IC50 = 19.74 μM) followed by β‐pinene, isoeugenol, eugenol, and estragole.
Daniella Issa; Amal Najjar; Hélène Greige-Gerges; Hala Nehme; Hala Nehme. Screening of Some Essential Oil Constituents as Potential Inhibitors of the ATP Synthase of Escherichia coli. Journal of Food Science 2018, 84, 138 -146.
AMA StyleDaniella Issa, Amal Najjar, Hélène Greige-Gerges, Hala Nehme, Hala Nehme. Screening of Some Essential Oil Constituents as Potential Inhibitors of the ATP Synthase of Escherichia coli. Journal of Food Science. 2018; 84 (1):138-146.
Chicago/Turabian StyleDaniella Issa; Amal Najjar; Hélène Greige-Gerges; Hala Nehme; Hala Nehme. 2018. "Screening of Some Essential Oil Constituents as Potential Inhibitors of the ATP Synthase of Escherichia coli." Journal of Food Science 84, no. 1: 138-146.
The effect of hydrogenation of phospholipids on the characteristics of freeze-dried liposomes was investigated using hydroxypropyl-ß-cyclodextrin (HP-ß-CD) as membrane protectant. The ethanol-injection method was applied to prepare liposomes using hydrogenated (Phospholopion-90H and 80H) and non-hydrogenated phospholipids (Lipoid-S100) in combination with cholesterol. Various liposomal formulations were tested: conventional liposomes (CL) and HP-ß-CD-loaded liposomes (CDL). Liposome suspensions were concentrated by ultracentrifugation; the pellets were reconstituted in water or CD solution and the dispersions were characterized for their size, polydispersity index and zeta potential. Results demonstrated that HP-ß-CD protected only the hydrogenated batches (CL and CDL) during freeze-drying. Moreover, the presence of HP-ß-CD in the aqueous phase of CDL protected them during freeze-drying. Freeze-dried CL and CDL made of phospholipon-90H loading anethole were demonstrated to be physically stable upon reconstitution in HP-ß-CD solutions, and are able to retain anethole after 6 months of storage at 4 °C thereby making them valuable for food applications.
Riham Gharib; Hélène Greige-Gerges; Sophie Fourmentin; Catherine Charcosset. Hydroxypropyl-ß-cyclodextrin as a membrane protectant during freeze-drying of hydrogenated and non-hydrogenated liposomes and molecule-in-cyclodextrin-in- liposomes: Application to trans-anethole. Food Chemistry 2018, 267, 67 -74.
AMA StyleRiham Gharib, Hélène Greige-Gerges, Sophie Fourmentin, Catherine Charcosset. Hydroxypropyl-ß-cyclodextrin as a membrane protectant during freeze-drying of hydrogenated and non-hydrogenated liposomes and molecule-in-cyclodextrin-in- liposomes: Application to trans-anethole. Food Chemistry. 2018; 267 ():67-74.
Chicago/Turabian StyleRiham Gharib; Hélène Greige-Gerges; Sophie Fourmentin; Catherine Charcosset. 2018. "Hydroxypropyl-ß-cyclodextrin as a membrane protectant during freeze-drying of hydrogenated and non-hydrogenated liposomes and molecule-in-cyclodextrin-in- liposomes: Application to trans-anethole." Food Chemistry 267, no. : 67-74.