Aging and degradation of 345 kV ethylene propylene diene monomer (EPDM) transmission line insulators removed from service is presented. These suspension type insulators were installed in a New Hampshire coastal area in 1995 and were removed in 2000 after unexplained outages in that structure. The purpose of this paper is to better understand the aging and degradation mechanisms of EPDM insulators in service. The insulators showed severe chalking and discoloration and partial loss of hydrophobicity on the side facing the sun. The surface structural changes were studied in detail using advanced surface analysis techniques, such as attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). For the first time, the significant differences in surface properties between the chalked/discolored (white) and the other surfaces (dark) were studied quantitatively. The Fourier transform infrared (FTIR) absorption spectra showed a significant decomposition of the CH groups of the white surface, elucidating the effect of photo-oxidation on the EPDM polymer. The SEM micrographs showed the cracking of the surfaces. The XPS spectra showed the formation of various polar carboxyl groups and the presence of high surface energy compounds, such as silica, and silicates. This study provided valuable basic information on the changes in the surface properties of EPDM insulators during service in a coastal environment.

Polymeric insulators have lightweight, excellent hydrophobicity and convenient transportation and installation. They are widely used in the external insulation for distribution and transmission lines. However, due to the long-term effects of pollution, ultraviolet radiation, discharge, temperature, humidity, altitude and other natural and complex environmental and service factors, the silicone rubber and other materials of polymeric insulators gradually age and lose their hydrophobicity and electrical insulation characteristics. The operability is significantly reduced, which seriously affects the safety and reliability of the power system. Hence, there is a need for assessing and evaluating the long-term aging and degradation of polymeric insulators under various operating conditions and environments. In this review, the various aging and characterization techniques of the polymeric insulators and their aging performance under the action of multiple factors are discussed. To enhance the performance of polymeric insulators, nano-coating, surface treatment and other techniques are also indicated. In addition, future potential fields that should be explored from a high-voltage electrical insulation perspective are also presented.

Triple negative breast cancer (TNBC) represents 15–20% of the over one million new breast cancer cases occurring each year. TNBC is an aggressive cancer phenotype, with low 5-year survival rates, high 3-year recurrence rates, and increased risk of metastasis. A lack of three commonly exploited hormone receptors renders TNBC resistant to endocrine therapies and lends to its critical absence of viable therapeutic targets. This necessitates the development of alternate and effective novel therapeutic strategies for TNBC. Towards this, our current work seeks to develop the technique of Electrical pulse (EP)-mediated Turmeric silver nanoparticles (TurNP) therapy, known as Electrochemotherapy (ECT), to effectively target TNBC cells. This technique involves the efficient delivery of natural bioactive molecules with anti-cancer effects via a biophysical means. In these experiments, the bioactive molecules are turmeric, a dried rhizome of Curcuma longa that has been used for centuries, both as a dietary supplement and as a medicine in Ayurveda (science of life) in the Indian subcontinent and in traditional Chinese medicine. Our results reveal the combined effect of TurNP + EP treatment in reducing MDA-MB-231 cell viability to as low as 9% at 12 h. Showing biological selectivity, this combination treatment has a substantially lower effect on non-tumorigenic mammary epithelial MCF10A cells (67% viability). To gain mechanistic insights into the actions of TurNP-based ECT treatment, we performed high-throughput, label-free quantitative proteomics studies. Proteomics results indicate that TurNP + EP treatment significantly influenced expression of a diverse list of proteins, including receptors, transcription factors, structural proteins, kinases, and metabolic enzymes. This include the downregulation of 25 proteins in PI3K-Akt signaling pathway (such as GRB2, EGFR, EPHA2, GNB1, GNB2, 14–3–3 family, and Integrin family proteins), and 12 proteins (AKR1A1, ALDOA, ALDOC, PGK1, PGM1, PGAM1, ENO1, ENO2, GAPDH, TPI1, LDHA, and LDHB) in the glycolytic pathway with concomitant reduction in metabolite levels (glucose uptake, and intracellular- lactate, glutamine, and glutamate). Compared to TurNP alone, TurNP + EP treatment upregulated 66 endoplasmic reticulum and 193 mitochondrial proteins, enhancing several processes and pathways, including Pyruvate Metabolism, Tricarboxylic acid (TCA) cycle, and Oxidative Phosphorylation (OXPHOS), which redirected the TNBC metabolism to mitochondria. This switch in the metabolism caused excessive production of H₂O₂ reactive oxygen species (ROS) to inflict cell death in MDA-MB-231 cells, demonstrating the potency of this treatment.