Accurately monitoring Canopy Nitrogen Concentration (CNC) is a prerequisite for precision nitrogen (N) fertiliser management at the farm scale with carbon and N budgeting across the landscape and ecosystems. While many spectral indices have been proposed for CNC monitoring, their applicability and accuracy are often adversely affected by confounding factors such as aboveground biomass (AGB), crop type, growth stages, and environmental conditions, limiting their broader application and adoption; with AGB being one of the most dominant signals and confounding factors at canopy scale. The confounding effect can become more challenging as AGB is also physiologically linked with CNC across the growth stages. Additionally, the interplay between index form, selection of optimal wavebands and their bandwidths remains poorly understood for CNC index design. This study proposes robust and cost-effective 2- and 4-waveband multispectral (MS) CNC indices applicable across a wide range of crop conditions. We collected 449 canopy reflectance spectra (400–980 nm) together with corresponding CNC and AGB measurements across four growth stages of ryegrass (winter and summer), and five growth stages of barley (winter-spring) in Victoria, Australia, in 2018 and 2019. All possible waveband (400–980 nm) combinations revealed that the best combination varied between seasons and crop types. However, the visible spectrum, particularly the blue region, presented high and consistent performance. Bandwidths of 10–40 nm outperformed either very narrow (2 nm) or very broad bandwidths (80 nm). The newly developed 2-waveband index (416 and 442 nm with 10-nm bandwidth; R² = 0.75 and NRMSE = 0.2) and 4-waveband index (512, 440, 414 and 588 nm with 40-nm bandwidth; R² = 0.81 and NRMSE = 0.17) exhibited the best performance, while validation with an independent dataset (from a different growing period to those used in the model development) obtained NRMSE values of 0.25 and 0.24, respectively. The 4-waveband index provides enhanced performance and permits use of broader bandwidths than its 2-waveband counterpart.

The soil-borne disease Verticillium wilt causes significant yield losses in cotton. Developing resistant cotton varieties is a long-term solution to manage the disease. The phytopathological parameter used to assess variety resistance is the presence or absence of infection, where transverse sectioning of infected stems is discoloured/brown. The traditional method of selecting resistant varieties involves manual scoring of visual stem discolouration after cutting. However, this process is associated with high labour costs, delayed processing time and cognitive biases. Therefore, automatic detection of resistant varieties with scalable, cost-effective, and rapid phenotyping tools is needed in cotton breeding.

Hyperspectral retrieval of nitrogen-related vegetation variables has undergone rapid methodological advances driven by the emergence of protein-sensitive radiative transfer models (RTMs), modern machine learning (ML), and operational imaging spectroscopy. This review synthesizes recent developments in hyperspectral retrieval of nitrogen-related vegetation variables across leaf and canopy scales, with particular emphasis on advances reported between 2020 and 2026. We examine the evolution from classical parametric regression and nonlinear ML approaches towards physically based RTM inversion and hybrid RTM–ML frameworks that integrate the complementary strengths of physical modeling and statistical learning. Particular attention is given to protein-sensitive RTMs, advanced ML approaches, and uncertainty-aware retrieval. Recent developments highlight the potential of hybrid RTM–ML frameworks to combine physical consistency with computationally efficient statistical learning, while probabilistic methods such as Gaussian Process Regression provide additional capabilities for uncertainty characterization. The review further discusses the transition from experimental studies to operational applications enabled by airborne and satellite imaging spectroscopy, including PRISMA, EnMAP, and forthcoming missions such as CHIME. Remaining challenges include the inherently ill-posed nature of nitrogen retrieval, limited and insufficiently representative calibration data, uncertainty characterization, and generalization across sensors, species, and ecosystems. Overall, the reviewed evidence points towards increasingly integrated retrieval frameworks, while emphasizing that robust transferability and operational implementation remain dependent on representative data, physical realism, and rigorous uncertainty assessment.