This page has only limited features, please log in for full access.
Saprolegnia infections are among the main parasitic diseases affecting farmed salmonids. The distribution and potential transfer of Saprolegnia spp. between farms and the natural environment has been scarcely investigated. Therefore, this work aimed to study the diversity and abundance of oomycete species in salmonid farms, tributary water, and effluent water systems. Four trout farms in Italy and two Atlantic salmon farms in Scotland were considered. In Italian farms, 532 isolates of oomycetes were obtained from fish and water, at upstream, inside, and downstream the farms. In Scottish farms, 201 oomycetes isolates were obtained from water outside the farm and from fish and water inside the farming units. Isolates were identified to the species level through amplification and sequencing of the ITS rDNA region. In Italy, S. parasitica was significantly more present in farmed than in wild fish, while in water it was more frequently isolated from the wild, particularly in effluent systems, not associated with more frequent isolation of S. parasitica in wild fish downstream the farm. In Scotland, S. parasitica was the most prevalent species isolated from fish, while isolates from water were mostly Pythium spp. with few S. parasitica isolates from upstream and downstream the farms.
Perla Tedesco; Marcia Saraiva; Jose Sandoval-Sierra; Maria Fioravanti; Benedetto Morandi; Javier Dieguez-Uribeondo; Pieter van West; Roberta Galuppi. Evaluation of Potential Transfer of the Pathogen Saprolegnia parasitica between Farmed Salmonids and Wild Fish. Pathogens 2021, 10, 926 .
AMA StylePerla Tedesco, Marcia Saraiva, Jose Sandoval-Sierra, Maria Fioravanti, Benedetto Morandi, Javier Dieguez-Uribeondo, Pieter van West, Roberta Galuppi. Evaluation of Potential Transfer of the Pathogen Saprolegnia parasitica between Farmed Salmonids and Wild Fish. Pathogens. 2021; 10 (8):926.
Chicago/Turabian StylePerla Tedesco; Marcia Saraiva; Jose Sandoval-Sierra; Maria Fioravanti; Benedetto Morandi; Javier Dieguez-Uribeondo; Pieter van West; Roberta Galuppi. 2021. "Evaluation of Potential Transfer of the Pathogen Saprolegnia parasitica between Farmed Salmonids and Wild Fish." Pathogens 10, no. 8: 926.
Cephalopods are intermediate/paratenic hosts in the life cycle of elasmobranch tapeworms, nevertheless most records of infection in this group of mollusks are outdated and fragmentary. The present work aimed to investigate the cestode fauna of the common octopus Octopus vulgaris from the Tyrrhenian Sea (Central Mediterranean). The parasitic stages were characterized by light and Scanning Electron Microscopy (SEM) and sequencing of 28S rDNA. Three cestode taxa were identified to the genus level: the onchoproteocephalidean Acanthobothrium sp. (prevalence 28%), the “tetraphyllidean” Anthobothrium sp. (prevalence 13%) and the trypanorhynch Nybelinia sp. (prevalence 3%). The remarkable prevalence observed for gastrointestinal cestodes highlight a possible important role of O. vulgaris in the transmission of elasmobranch tapeworms, particularly Onchoproteocephalideans. Furthermore, the present work provides, for the first time, detailed morphological (SEM) and molecular support to confirm the occurrence of Anthobothrium sp. in cephalopod hosts. In order to gain higher taxonomic resolution for the identified taxa, we stress the need to collect further morphological and molecular data of adult cestodes infecting their elasmobranch definitive hosts.
Perla Tedesco; Monica Caffara; Andrea Gustinelli; Graziano Fiorito; Maria Letizia Fioravanti. Metacestodes of Elasmobranch Tapeworms in Octopus vulgaris (Mollusca, Cephalopoda) from Central Mediterranean—SEM and Molecular Data. Animals 2020, 10, 2038 .
AMA StylePerla Tedesco, Monica Caffara, Andrea Gustinelli, Graziano Fiorito, Maria Letizia Fioravanti. Metacestodes of Elasmobranch Tapeworms in Octopus vulgaris (Mollusca, Cephalopoda) from Central Mediterranean—SEM and Molecular Data. Animals. 2020; 10 (11):2038.
Chicago/Turabian StylePerla Tedesco; Monica Caffara; Andrea Gustinelli; Graziano Fiorito; Maria Letizia Fioravanti. 2020. "Metacestodes of Elasmobranch Tapeworms in Octopus vulgaris (Mollusca, Cephalopoda) from Central Mediterranean—SEM and Molecular Data." Animals 10, no. 11: 2038.
We compiled an updated global catalogue of parasites in cephalopods. Data were used to assess changes in taxonomic distinctness of parasites over two centuries and across the world’s oceans, to quantify turnover and nestedness components of parasite β-diversity, and to attempt estimating their γ-diversity at a global scale. A total of 309 parasites infecting 164 cephalopods were found. We hypothesize that this diversity counts for less than half the potential parasite richness in this molluscan taxon. Taxonomic breadth of parasites was significantly above expectations from null models for Mediterranean Sea and NE Atlantic Ocean, whereas the opposite occurred for NW Pacific Ocean, where a few closely related genera characterized the parasite pool. β-diversity of parasites was very high and dominated by turnover, except for the Atlantic Ocean where a nested pattern among sub-basins emerged. Taxonomic relatedness of parasite species remained substantially unchanged through time, but species replacements largely occurred over the last two centuries. Our findings highlighted potential hotspots of taxonomic distinctness in cephalopod parasites, geographic regions deserving future research, and the need for a deeper understanding of the magnitude of marine parasite diversity, their biogeography, and their role in marine ecosystems. Our global overview may represent a baseline step for future advances in this direction.
Perla Tedesco; Stanislao Bevilacqua; Graziano Fiorito; Antonio Terlizzi. Global patterns of parasite diversity in cephalopods. Scientific Reports 2020, 10, 1 -11.
AMA StylePerla Tedesco, Stanislao Bevilacqua, Graziano Fiorito, Antonio Terlizzi. Global patterns of parasite diversity in cephalopods. Scientific Reports. 2020; 10 (1):1-11.
Chicago/Turabian StylePerla Tedesco; Stanislao Bevilacqua; Graziano Fiorito; Antonio Terlizzi. 2020. "Global patterns of parasite diversity in cephalopods." Scientific Reports 10, no. 1: 1-11.
The fish parasites Saprolegnia spp. (Oomycota) and Amyloodinium ocellatum (Dinophyceae) cause important losses in freshwater and marine aquaculture industry, respectively. The possible adverse effects of compounds used to control these parasites in aquaculture resulted in increased interest on the search for natural products with antiparasitic activity. In this work, eighteen plant-derived compounds (2′,4′-Dihydroxychalcone; 7-Hydroxyflavone; Artemisinin; Camphor (1R); Diallyl sulfide; Esculetin; Eucalyptol; Garlicin 80%; Harmalol hydrochloride dihydrate; Palmatine chloride; Piperine; Plumbagin; Resveratrol; Rosmarinic acid; Sclareolide; Tomatine, Umbelliferone, and Usnic Acid) have been tested in vitro. Sixteen of these were used to determine their effects on the gill cell line G1B (ATCC®CRL-2536™) and on the motility of viable dinospores of Amyloodinium ocellatum, and thirteen were screened for inhibitory activity against Saprolegnia spp. The cytotoxicity results on G1B cells determined that only two compounds (2′,4′-Dihydroxychalcone and Tomatine) exhibited dose-dependent toxic effects. The highest surveyed concentrations (0.1 and 0.01 mM) reduced cell viability by 80%. Upon lowering the compound concentration the percentage of dead cells was lower than 20%. The same two compounds revealed to be potential antiparasitics by reducing in a dose-dependent manner the motility of A. ocellatum dinospores up to 100%. With respect to Saprolegnia, a Minimum Inhibitory Concentration was found for Tomatine (0.1 mM), Piperine and Plumbagin (0.25 mM), while 2′,4′-Dihydroxychalcone considerably slowed down mycelial growth for 24 h at a concentration of 0.1 mM. Therefore, this research allowed to identify two compounds, Tomatine and 2′,4′-Dihydroxychalcone, effective against both parasites. These compounds could represent promising candidates for the treatment of amyloodiniosis and saprolegniosis in aquaculture. Nevertheless, further in vitro and in vivo tests are required in order to determine concentrations that are effective against the considered pathogens but at the same time safe for hosts, environment and consumers.
Perla Tedesco; Paola Beraldo; Michela Massimo; Maria Letizia Fioravanti; Donatella Volpatti; Ron Dirks; Roberta Galuppi. Comparative Therapeutic Effects of Natural Compounds Against Saprolegnia spp. (Oomycota) and Amyloodinium ocellatum (Dinophyceae). Frontiers in Veterinary Science 2020, 7, 1 .
AMA StylePerla Tedesco, Paola Beraldo, Michela Massimo, Maria Letizia Fioravanti, Donatella Volpatti, Ron Dirks, Roberta Galuppi. Comparative Therapeutic Effects of Natural Compounds Against Saprolegnia spp. (Oomycota) and Amyloodinium ocellatum (Dinophyceae). Frontiers in Veterinary Science. 2020; 7 ():1.
Chicago/Turabian StylePerla Tedesco; Paola Beraldo; Michela Massimo; Maria Letizia Fioravanti; Donatella Volpatti; Ron Dirks; Roberta Galuppi. 2020. "Comparative Therapeutic Effects of Natural Compounds Against Saprolegnia spp. (Oomycota) and Amyloodinium ocellatum (Dinophyceae)." Frontiers in Veterinary Science 7, no. : 1.
In the Afrotropic region, the genus Clinostomum is represented by four accepted and four unnamed species distinguished using molecular data. Here, we describe one of the four unnamed species as Clinostomum ukolii n. sp. based on metacercariae from siluriform fishes (Synodontis batensoda, Schilbe intermedius) collected in Nigeria and South Africa. The new species is distinguished by molecular data (39 new sequences of partial cytochrome c oxidase I ≥ 6.7% divergent from those of other species) and morphological differences from named and unnamed species in the same region. Metacercariae of C. ukolii n. sp. can be distinguished based on size, tegumental spines, and various aspects of the genital complex, including its position, lobation of the anterior testis, and the disposition and shape of the cirrus pouch. Although descriptions of new species of digeneans are typically based on the morphology of adults, we argue that in cases where data are available from metacercariae from regionally known species, new species can be described based on metacercariae, particularly when supported by molecular data, as here. Moreover, sub-adult reproductive structures can be clearly visualized in metacercaria of Clinostomum. Considering metacercariae as potential types for new species could advance clinostome systematics more rapidly, because metacercariae are encountered much more often than adults in avian definitive hosts.
Monica Caffara; Sean A. Locke; Paul C. Echi; Ali Halajian; Willem J. Luus-Powell; Deborah Benini; Perla Tedesco; Maria L. Fioravanti. A new species of Clinostomum Leidy, 1856 based on molecular and morphological analysis of metacercariae from African siluriform fishes. Parasitology Research 2020, 119, 885 -892.
AMA StyleMonica Caffara, Sean A. Locke, Paul C. Echi, Ali Halajian, Willem J. Luus-Powell, Deborah Benini, Perla Tedesco, Maria L. Fioravanti. A new species of Clinostomum Leidy, 1856 based on molecular and morphological analysis of metacercariae from African siluriform fishes. Parasitology Research. 2020; 119 (3):885-892.
Chicago/Turabian StyleMonica Caffara; Sean A. Locke; Paul C. Echi; Ali Halajian; Willem J. Luus-Powell; Deborah Benini; Perla Tedesco; Maria L. Fioravanti. 2020. "A new species of Clinostomum Leidy, 1856 based on molecular and morphological analysis of metacercariae from African siluriform fishes." Parasitology Research 119, no. 3: 885-892.
Metacercariae of Clinostomum Leidy, 1856 are frequently encountered in freshwater fish. In 2015, a provisional species of Clinostomum in People’s Republic of China (PRC) was distinguished from C. complanatum (Rudolphi, 1819) in Europe based on divergent cytochrome c oxidase I (CO1). However, in subsequent studies in East Asia, the same divergent CO1 genotype was identified as C. complanatum. These matching sequences suggest that either the provisional East Asian species was incorrectly distinguished from C. complanatum in 2015 or that C. complanatum in East Asia was misidentified in later studies. We tested these alternatives by sequencing the mitochondrial genome of C. complanatum in Italy, which was 5.7% divergent from a previously published sequence from Clinostomum in PRC, including differences in 80 of 3390 (2.4%) translated amino acids. Partial CO1 sequences of specimens from PRC and those from Italy, Romania, and Turkey also each formed reciprocally monophyletic clades. Partial CO1 from the East Asian clade varied by mean 3.6% (range 2.4–4.8%) from C. complanatum from Italy, Romania, and Turkey; mean intra-clade CO1 variation was 0.3% (range 0–1.9%). Metacercariae from Europe and East Asia display significant morphometric variation, and data from the literature suggest morphological differences in the genital complex of adults. Although sequences of nuclear rDNA did not differ between isolates from the west and East Asia, taken together, these results lead us to describe a new species of Clinostomum.
Sean A. Locke; Monica Caffara; Daniel Barčák; Pasaikou Sonko; Perla Tedesco; Maria L. Fioravanti; Wenxiang Li. A new species of Clinostomum Leidy, 1856 in East Asia based on genomic and morphological data. Parasitology Research 2019, 118, 3253 -3265.
AMA StyleSean A. Locke, Monica Caffara, Daniel Barčák, Pasaikou Sonko, Perla Tedesco, Maria L. Fioravanti, Wenxiang Li. A new species of Clinostomum Leidy, 1856 in East Asia based on genomic and morphological data. Parasitology Research. 2019; 118 (12):3253-3265.
Chicago/Turabian StyleSean A. Locke; Monica Caffara; Daniel Barčák; Pasaikou Sonko; Perla Tedesco; Maria L. Fioravanti; Wenxiang Li. 2019. "A new species of Clinostomum Leidy, 1856 in East Asia based on genomic and morphological data." Parasitology Research 118, no. 12: 3253-3265.
The fan mussel, Pinna nobilis (Linnaeus 1758), is an endemic bivalve of the Mediterranean basin, protected by international legislation as an endangered species. In the early summer of 2018, a mass mortality event (MME) of P. nobilis was recorded in the Gulf of Taranto (Southern Italy, Ionian Sea). Moribund specimens of P. nobilis were collected by scuba divers and processed by bacteriological, parasitological, histopathological and molecular analyses to investigate the causes of this MME. Different developmental stages (i.e., plasmodia, spores and sporocysts) of a presumptive haplosporidian parasite were observed during the histological analysis in the epithelium and in the lumen of the digestive tubules, where mature spores occurred either free or in sporocysts. The spores presented an operculum and an ovoid shape measuring 4.4 µm (±0.232) in length and 3.6 µm (± 0.233) in width. BLAST analysis of an 18SrRNA sequence revealed a high nucleotide similarity (99%) with the reference sequence of Haplosporidium pinnae available in GenBank database. Phylogenetic analysis using the 18SrRNA sequence clustered the pathogen in a paraphyletic clade with the reference sequence of H. pinnae, excluding other haplosporidians (i.e., Bonamia and Minchinia genera). Based on data reported, H. pinnae was the causative agent of MME in the populations of P. nobilis sampled in the Ionian Sea, where the conservation of this endangered species is heavily threatened by such a protozoan infection. Further investigations should contribute to knowledge about the life cycle of H. pinnae in order to reduce spread of the pathogen and to mitigate the burden of the disease where P. nobilis is facing the risk of extinction.
Rossella Panarese; Perla Tedesco; Giovanni Chimienti; Maria Stefania Latrofa; Francesco Quaglio; Giuseppe Passantino; Canio Buonavoglia; Andrea Gustinelli; Angelo Tursi; Domenico Otranto. Haplosporidium pinnae associated with mass mortality in endangered Pinna nobilis (Linnaeus 1758) fan mussels. Journal of Invertebrate Pathology 2019, 164, 32 -37.
AMA StyleRossella Panarese, Perla Tedesco, Giovanni Chimienti, Maria Stefania Latrofa, Francesco Quaglio, Giuseppe Passantino, Canio Buonavoglia, Andrea Gustinelli, Angelo Tursi, Domenico Otranto. Haplosporidium pinnae associated with mass mortality in endangered Pinna nobilis (Linnaeus 1758) fan mussels. Journal of Invertebrate Pathology. 2019; 164 ():32-37.
Chicago/Turabian StyleRossella Panarese; Perla Tedesco; Giovanni Chimienti; Maria Stefania Latrofa; Francesco Quaglio; Giuseppe Passantino; Canio Buonavoglia; Andrea Gustinelli; Angelo Tursi; Domenico Otranto. 2019. "Haplosporidium pinnae associated with mass mortality in endangered Pinna nobilis (Linnaeus 1758) fan mussels." Journal of Invertebrate Pathology 164, no. : 32-37.
The genus Clinostomoides Dollfus, 1950 was erected to accommodate a single worm from Ardea goliath sampled in the Belgian Congo. The specimen was distinguished from other clinostomids by its large size and posterior genitalia. In the following years, metacercariae of Clinostomoides brieni, have been described in Clarias spp. in southern and western Africa. A few authors have referred to Clinostomum brieni, but all such usages appear to be lapsus calami, and the validity of Clinostomoides remains widely accepted. In this study our aim was: position C. brieni among the growing clinostomids molecular database, and redescribe the species with emphasis on characters that have emerged as important in recent work. We sequenced two nuclear (partial 18S and ITS) and one mitochondrial marker (partial cytochrome c oxidase I) and studied morphology in metacercariae from hosts and localities likely to harbour the type species (Clarias spp., Democratic Republic of the Congo, South Africa). Phylogenetic analysis shows C. brieni belongs within Clinostomum Leidy, 1856. We therefore transfer C. brieni to Clinostomum, amend the diagnosis for the genus Clinostomum and provide a critical analysis of other species in Clinostomoides, all of which we consider species inquirendae, as they rest on comparisons of different developmental stages.
Monica Caffara; Sean A. Locke; Ali Halajian; Wilmien J. Luus-Powell; Deborah Benini; Perla Tedesco; Gyrhaiss Kapepula Kasembele; Maria L. Fioravanti. Molecular data show Clinostomoides Dollfus, 1950 is a junior synonym of Clinostomum Leidy, 1856, with redescription of metacercariae of Clinostomum brieni n. comb. Parasitology 2019, 146, 805 -813.
AMA StyleMonica Caffara, Sean A. Locke, Ali Halajian, Wilmien J. Luus-Powell, Deborah Benini, Perla Tedesco, Gyrhaiss Kapepula Kasembele, Maria L. Fioravanti. Molecular data show Clinostomoides Dollfus, 1950 is a junior synonym of Clinostomum Leidy, 1856, with redescription of metacercariae of Clinostomum brieni n. comb. Parasitology. 2019; 146 (6):805-813.
Chicago/Turabian StyleMonica Caffara; Sean A. Locke; Ali Halajian; Wilmien J. Luus-Powell; Deborah Benini; Perla Tedesco; Gyrhaiss Kapepula Kasembele; Maria L. Fioravanti. 2019. "Molecular data show Clinostomoides Dollfus, 1950 is a junior synonym of Clinostomum Leidy, 1856, with redescription of metacercariae of Clinostomum brieni n. comb." Parasitology 146, no. 6: 805-813.
Oomycetes of the genus Saprolegnia are responsible for severe economic losses in freshwater aquaculture. Following the ban of malachite green in food fish production, the demand for new treatments pushes towards the selection of more safe and environment-friendly products. In the present work, in vitro activity of ten chemicals and three commercial products was tested on different strains of Saprolegnia, using malachite green as reference compound. The compounds were screened in agar and in water to assess the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC), respectively. Two strains of Saprolegnia parasitica and one isolate of Saprolegnia delica were tested in triplicate per each concentration. Among tested chemicals, benzoic acid showed the lowest MIC (100 ppm) followed by acetic acid, iodoacetic acid and copper sulphate (250 ppm). Sodium percarbonate was not effective at any tested concentration. Among commercial products, Virkon S was effective in inhibiting the growth of the mycelium (MIC = MLC = 1,000 ppm). Actidrox® and Detarox® AP showed MIC = 5,000 and 1,000 ppm, respectively, while MLCs were 10-fold lower than MICs, possibly due to a higher activity of these products in water. Similarly, a higher effectiveness in water was observed also for iodoacetic acid.
Perla Tedesco; Maria Letizia Fioravanti; Roberta Galuppi. In vitro activity of chemicals and commercial products againstSaprolegnia parasiticaandSaprolegnia delicastrains. Journal of Fish Diseases 2018, 42, 237 -248.
AMA StylePerla Tedesco, Maria Letizia Fioravanti, Roberta Galuppi. In vitro activity of chemicals and commercial products againstSaprolegnia parasiticaandSaprolegnia delicastrains. Journal of Fish Diseases. 2018; 42 (2):237-248.
Chicago/Turabian StylePerla Tedesco; Maria Letizia Fioravanti; Roberta Galuppi. 2018. "In vitro activity of chemicals and commercial products againstSaprolegnia parasiticaandSaprolegnia delicastrains." Journal of Fish Diseases 42, no. 2: 237-248.
Raphidascarididae are among the most abundant and widespread parasitic nematodes in the marine environment. Knowledge about the life-cycle of most raphidascaridid species is still poorly known and information about their distribution and host range is lacking in many geographical areas, as well as the taxonomy of several species. A study of larval and adult stages of Hysterothylacium fabri (Rudolphi, 1819) Deardorff & Overstreet, 1980 (Nematoda: Raphidascarididae) infecting the striped goatfish Mullus surmuletus Linnaeus, 1758 (Mullidae) and the Mediterranean stargazer Uranoscopus scaber Linnaeus, 1759 (Uranoscopidae) from Ionian Sea (central Mediterranean) has been carried out by combining light and scanning electron microscopy observations and molecular analyses through PCR-RFLP and sequencing of the ITS rDNA gene. Results indicate that U. scaber and M. surmuletus represent suitable definitive and intermediate/paratenic hosts of H. fabri respectively in the Mediterranean and highlight the importance of combining genetic and morphological data to study the taxonomy and epidemiology of parasites widely distributed in different fish species and aquatic ecosystems.
Perla Tedesco; Andrea Gustinelli; Monica Caffara; Pierpaolo Patarnello; Antonio Terlizzi; Maria L. Fioravanti. Hysterothylacium fabri(Nematoda: Raphidascarididae) inMullus surmuletus(Perciformes: Mullidae) andUranoscopus scaber(Perciformes: Uranoscopidae) from the Mediterranean. Journal of Parasitology 2018, 104, 262 -274.
AMA StylePerla Tedesco, Andrea Gustinelli, Monica Caffara, Pierpaolo Patarnello, Antonio Terlizzi, Maria L. Fioravanti. Hysterothylacium fabri(Nematoda: Raphidascarididae) inMullus surmuletus(Perciformes: Mullidae) andUranoscopus scaber(Perciformes: Uranoscopidae) from the Mediterranean. Journal of Parasitology. 2018; 104 (3):262-274.
Chicago/Turabian StylePerla Tedesco; Andrea Gustinelli; Monica Caffara; Pierpaolo Patarnello; Antonio Terlizzi; Maria L. Fioravanti. 2018. "Hysterothylacium fabri(Nematoda: Raphidascarididae) inMullus surmuletus(Perciformes: Mullidae) andUranoscopus scaber(Perciformes: Uranoscopidae) from the Mediterranean." Journal of Parasitology 104, no. 3: 262-274.
The gastric ganglion is the largest visceral ganglion in cephalopods. It is connected to the brain and is implicated in regulation of digestive tract functions. Here we have investigated the neurochemical complexity (through in silico gene expression analysis and immunohistochemistry) of the gastric ganglion in Octopus vulgaris and tested whether the expression of a selected number of genes was influenced by the magnitude of digestive tract parasitic infection by Aggregata octopiana. Novel evidence was obtained for putative peptide and non-peptide neurotransmitters in the gastric ganglion: cephalotocin, corticotrophin releasing factor, FMRFamide, gamma amino butyric acid, 5-hydroxytryptamine, molluscan insulin-related peptide 3, peptide PRQFV-amide, and tachykinin–related peptide. Receptors for cholecystokininA and cholecystokininB, and orexin2 were also identified in this context for the first time. We report evidence for acetylcholine, dopamine, noradrenaline, octopamine, small cardioactive peptide related peptide, and receptors for cephalotocin and octopressin, confirming previous publications. The effects of Aggregata observed here extend those previously described by showing effects on the gastric ganglion; in animals with a higher level of infection, genes implicated in inflammation (NFκB, fascin, serpinB10 and the toll-like 3 receptor) increased their relative expression, but TNF-α gene expression was lower as was expression of other genes implicated in oxidative stress (i.e., superoxide dismutase, peroxiredoxin 6, and glutathione peroxidase). Elevated Aggregata levels in the octopuses corresponded to an increase in the expression of the cholecystokininA receptor and the small cardioactive peptide-related peptide. In contrast, we observed decreased relative expression of cephalotocin, dopamine β-hydroxylase, peptide PRQFV-amide, and tachykinin-related peptide genes. A discussion is provided on (i) potential roles of the various molecules in food intake regulation and digestive tract motility control and (ii) the difference in relative gene expression in the gastric ganglion in octopus with relatively high and low parasitic loads and the similarities to changes in the enteric innervation of mammals with digestive tract parasites. Our results provide additional data to the described neurochemical complexity of O. vulgaris gastric ganglion.
Elena Baldascino; Giulia Di Cristina; Perla Tedesco; Carl Hobbs; Tanya J. Shaw; Giovanna Ponte; Paul L. R. Andrews. The Gastric Ganglion of Octopus vulgaris: Preliminary Characterization of Gene- and Putative Neurochemical-Complexity, and the Effect of Aggregata octopiana Digestive Tract Infection on Gene Expression. Frontiers in Physiology 2017, 8, 1 .
AMA StyleElena Baldascino, Giulia Di Cristina, Perla Tedesco, Carl Hobbs, Tanya J. Shaw, Giovanna Ponte, Paul L. R. Andrews. The Gastric Ganglion of Octopus vulgaris: Preliminary Characterization of Gene- and Putative Neurochemical-Complexity, and the Effect of Aggregata octopiana Digestive Tract Infection on Gene Expression. Frontiers in Physiology. 2017; 8 ():1.
Chicago/Turabian StyleElena Baldascino; Giulia Di Cristina; Perla Tedesco; Carl Hobbs; Tanya J. Shaw; Giovanna Ponte; Paul L. R. Andrews. 2017. "The Gastric Ganglion of Octopus vulgaris: Preliminary Characterization of Gene- and Putative Neurochemical-Complexity, and the Effect of Aggregata octopiana Digestive Tract Infection on Gene Expression." Frontiers in Physiology 8, no. : 1.
Coccidian parasites of the genus Aggregata are known to parasitize cephalopods as definitive hosts, however one of the genus members, A. octopiana, has shown an unresolved phylogeny within the same definitive host, the common octopus (Octopus vulgaris). Our study represents a large-scale investigation aimed at characterizing morphological traits and phylogeny of A. octopiana isolated from O. vulgaris inhabiting three distinct geographic areas of the central Mediterranean: The Adriatic, Ionian and Tyrrhenian Seas. The morphology of sporogonic stages of the parasite in octopus tissues was assessed by light and electron microscopy; molecular characterization has been carried out using the 18S rRNA locus. Our results support the hypothesis that two morphologically and genetically different A. octopiana infect O. vulgaris in the investigated areas of the Mediterranean Sea. Additional nuclear and mitochondrial markers for Aggregata should provide further information and better resolution of its phylogeny.
Perla Tedesco; Camino Gestal; Kristina Begić; Ivona Mladineo; Sheila Castellanos-Martínez; Gaetano Catanese; Antonio Terlizzi; Graziano Fiorito. Morphological and Molecular Characterization of Aggregata spp. Frenzel 1885 (Apicomplexa: Aggregatidae) in Octopus vulgaris Cuvier 1797 (Mollusca: Cephalopoda) from Central Mediterranean. Protist 2017, 168, 636 -648.
AMA StylePerla Tedesco, Camino Gestal, Kristina Begić, Ivona Mladineo, Sheila Castellanos-Martínez, Gaetano Catanese, Antonio Terlizzi, Graziano Fiorito. Morphological and Molecular Characterization of Aggregata spp. Frenzel 1885 (Apicomplexa: Aggregatidae) in Octopus vulgaris Cuvier 1797 (Mollusca: Cephalopoda) from Central Mediterranean. Protist. 2017; 168 (5):636-648.
Chicago/Turabian StylePerla Tedesco; Camino Gestal; Kristina Begić; Ivona Mladineo; Sheila Castellanos-Martínez; Gaetano Catanese; Antonio Terlizzi; Graziano Fiorito. 2017. "Morphological and Molecular Characterization of Aggregata spp. Frenzel 1885 (Apicomplexa: Aggregatidae) in Octopus vulgaris Cuvier 1797 (Mollusca: Cephalopoda) from Central Mediterranean." Protist 168, no. 5: 636-648.
Cephalopods are the sole invertebrates included in the list of regulated species following the Directive 2010/63/EU. According to the Directive, achieving competence through adequate training is a requisite for people having a role in the different functions (article 23) as such carrying out procedures on animals, designing procedures and projects, taking care of animals, killing animals. Cephalopod Biology and Care Training Program is specifically designed to comply with the requirements of the “working document on the development of a common education and training framework to fulfil the requirements under the Directive 2010/63/EU”. The training event occurred at the ICM-CSIC in Barcelona (Spain) where people coming from Europe, America and Asia were instructed on how to cope with regulations for the use of cephalopod molluscs for scientific purposes. The training encompasses discussion on the guidelines for the use and care of animals and their welfare with particular reference to procedures that may be of interest for neuroscience. Intensive discussion has been carried out during the training sessions with focus on behavioural studies and paradigms, welfare assessment, levels of severity of scientific procedures, animal care, handling, transport, individual identification and marking, substance administration, anaesthesia, analgesia and humane killing.
Vanessa Lopes; Eduardo Sampaio; Katina Roumbedakis; Nobuaki K. Tanaka; Lucía Carulla; Guillermo Gambús; Theodosia Woo; Catarina P. P. Martins; Virginie Penicaud; Colette Gibbings; Jessica Eberle; Perla Tedesco; Isabel Fernández; Tania Rodríguez-González; Pamela Imperadore; Giovanna Ponte; Graziano Fiorito. Cephalopod biology and care, a COST FA1301 (CephsInAction) training school: anaesthesia and scientific procedures. Invertebrate Neuroscience 2017, 17, 1 .
AMA StyleVanessa Lopes, Eduardo Sampaio, Katina Roumbedakis, Nobuaki K. Tanaka, Lucía Carulla, Guillermo Gambús, Theodosia Woo, Catarina P. P. Martins, Virginie Penicaud, Colette Gibbings, Jessica Eberle, Perla Tedesco, Isabel Fernández, Tania Rodríguez-González, Pamela Imperadore, Giovanna Ponte, Graziano Fiorito. Cephalopod biology and care, a COST FA1301 (CephsInAction) training school: anaesthesia and scientific procedures. Invertebrate Neuroscience. 2017; 17 (3):1.
Chicago/Turabian StyleVanessa Lopes; Eduardo Sampaio; Katina Roumbedakis; Nobuaki K. Tanaka; Lucía Carulla; Guillermo Gambús; Theodosia Woo; Catarina P. P. Martins; Virginie Penicaud; Colette Gibbings; Jessica Eberle; Perla Tedesco; Isabel Fernández; Tania Rodríguez-González; Pamela Imperadore; Giovanna Ponte; Graziano Fiorito. 2017. "Cephalopod biology and care, a COST FA1301 (CephsInAction) training school: anaesthesia and scientific procedures." Invertebrate Neuroscience 17, no. 3: 1.
A redescription of Philometra globiceps (Rudolphi 1819), the type species of Philometra Costa 1845 (Philometridae), is provided on the basis of specimens collected from the gonads, stomach, pyloric caeca, intestine and gallbladder of the marine fish Uranoscopus scaber Linnaeus (Uranoscopidae, Perciformes) caught in the Ionian Sea off Ugento, southern Italy. Light microscopical and scanning electron microscopical examinations (latter used for the first time in this species) of the specimens revealed some previously unreported morphological features, such as the location of submedian pairs of cephalic papillae of external circle on four elevated cuticular lobes in gravid females, the presence of amphids, genital papillae and phasmids in males, the lamellate structure of the distal end of gubernaculum appearing as a dorsal protuberance in lateral view and the structure of the male caudal end. A taxonomically important feature of P. globiceps is the details in the dorsal transverse lamella-like structures on the distal end of the gubernaculum, by which this species can be separated from other gonad-infecting species of this genus. Apparently, P. globiceps is a specific parasite of U. scaber and all previous records of this species from hosts belonging to other fish families are evidently based on misidentifications.
František Moravec; Perla Tedesco. Redescription of Philometra globiceps (Rudolphi, 1819) (Nematoda: Philometridae), the type species of Philometra Costa, 1845, including the first data obtained by SEM. Parasitology Research 2015, 114, 4413 -4420.
AMA StyleFrantišek Moravec, Perla Tedesco. Redescription of Philometra globiceps (Rudolphi, 1819) (Nematoda: Philometridae), the type species of Philometra Costa, 1845, including the first data obtained by SEM. Parasitology Research. 2015; 114 (12):4413-4420.
Chicago/Turabian StyleFrantišek Moravec; Perla Tedesco. 2015. "Redescription of Philometra globiceps (Rudolphi, 1819) (Nematoda: Philometridae), the type species of Philometra Costa, 1845, including the first data obtained by SEM." Parasitology Research 114, no. 12: 4413-4420.
Betanodaviruses are the causative agents of Viral Encephalopathy and Retinopathy (VER). To date, more than 50 species have proved to be susceptible and among them, those found in genus Epinephelus are highly represented. Clinical disease outbreaks are generally characterized by typical nervous signs and significant mortalities mainly associated with aquaculture activities, although some concerns for the impact of this infection in wild fish have been raised. In this study, the authors present the first documented report describing an outbreak of VER in wild species in the Mediterranean basin.
Niccolò Vendramin; Pierpaolo Patarnello; Anna Toffan; Valentina Panzarin; Elisabetta Cappellozza; Perla Tedesco; Antonio Terlizzi; Calogero Terregino; Giovanni Cattoli. Viral Encephalopathy and Retinopathy in groupers (Epinephelus spp.) in southern Italy: a threat for wild endangered species? BMC Veterinary Research 2013, 9, 20 -20.
AMA StyleNiccolò Vendramin, Pierpaolo Patarnello, Anna Toffan, Valentina Panzarin, Elisabetta Cappellozza, Perla Tedesco, Antonio Terlizzi, Calogero Terregino, Giovanni Cattoli. Viral Encephalopathy and Retinopathy in groupers (Epinephelus spp.) in southern Italy: a threat for wild endangered species? BMC Veterinary Research. 2013; 9 (1):20-20.
Chicago/Turabian StyleNiccolò Vendramin; Pierpaolo Patarnello; Anna Toffan; Valentina Panzarin; Elisabetta Cappellozza; Perla Tedesco; Antonio Terlizzi; Calogero Terregino; Giovanni Cattoli. 2013. "Viral Encephalopathy and Retinopathy in groupers (Epinephelus spp.) in southern Italy: a threat for wild endangered species?" BMC Veterinary Research 9, no. 1: 20-20.
Antonio Terlizzi; Perla Tedesco; Pierpaolo Patarnello. Spread of Pathogens from Marine Cage Aquaculture - A Potential Threat for Wild Fish Assemblages Under Protection Regimes? Health and Environment in Aquaculture 2012, 1 .
AMA StyleAntonio Terlizzi, Perla Tedesco, Pierpaolo Patarnello. Spread of Pathogens from Marine Cage Aquaculture - A Potential Threat for Wild Fish Assemblages Under Protection Regimes? Health and Environment in Aquaculture. 2012; ():1.
Chicago/Turabian StyleAntonio Terlizzi; Perla Tedesco; Pierpaolo Patarnello. 2012. "Spread of Pathogens from Marine Cage Aquaculture - A Potential Threat for Wild Fish Assemblages Under Protection Regimes?" Health and Environment in Aquaculture , no. : 1.