To understand the effects of variable water masses in the northeastern East China Sea (Korea South Sea), planktonic copepods were seasonally sampled. Out of a total of 106 copepod species, 85 were oceanic warm-water species, and the number of species varied in summer, autumn, spring, and winter. The study area was divided into two or three regions according to the degree of influence of the water masses. Canthocalanus pauper, Clausocalanus furcatus, Oithona plumifera, Oncaea venella, Oncaea venusta, and Paracalanus aculeatus showed a positive correlation with water temperature and salinity and were indicator species of warm currents. Calanus sinicus, known as an indicator species of the Yellow Sea Bottom Cold Water, showed a high abundance and occurrence ratio in the western sea of the study area from spring to autumn. Moreover, Acartia pacifica indicated the extension of coastal waters to offshore areas. Several oceanic warm-water species (A. danae, Centropages gracilis, Labidocera acuta, Rhincalanus nasutus, and Temoropia mayumbaensis) were considered indicator species of the Taiwan Warm Current. Our results suggest that the spatiotemporal distribution patterns of indicator species are partly explained by different water masses.

The recent accelerated ocean acidification and global warming caused by increased atmospheric carbon dioxide may have an impact on the physiology and ecology of marine animals. This study was conducted to determine the egg production rate (EPR) and hatching success (EHS) of Acartia ohtsukai in response to the combined effects of an increase in temperature and a lower pH. Acartiaohtsukai with fresh surface seawater were collected in the northwestern Yeoja Bay of Korea in September 2017. The temperature and pH conditions applied included two different pH levels (representing the present: 7.9 and the future: 7.6) and three temperature values (26 °C, 28 °C, and 30 °C). In the pH 7.9, EPR significantly increased with increased temperature, but in pH 7.6, it significantly decreased as the temperature increased. EHS was lower in pH 7.6 than in pH 7.9. These results suggest that changes in the marine environment due to global warming and ocean acidification may affect Acartia populations and cause overall fluctuations in copepods of the genus Acartia.

The effects of the addition of nutrients (nitrate: N; phosphate: P; and vitamin B₁) and trace metals (iron: Fe; Copper: Cu; and selenium: Se) on the growth of Gymnodinium catenatum, which was isolated from Korean coastal waters, were investigated. The Korean isolate of G. catenatum grew under a wide range of concentrations of N and P. Whilst high concentrations of N (> N: P ratio of 23.5) did not stimulate the growth rate, an enhanced growth rate and cell density were observed with the addition of P. The experimental addition of vitamin B₁ revealed that G. catenatum is not dependent on vitamin B₁ for growth. Moreover, the addition of Fe and Cu resulted in no significant differences in the growth patterns and rates of G. catenatum between the controls and treatments. It is thus possible that growth of the Korean isolate of G. catenatum does not require high concentrations of Fe and Cu. However, the cell densities were enhanced in the stationary phases of treatments upon addition of Se, and the maximum cell densities were higher than those in the culture experiments upon additions of other nutrient and trace metals. Our findings indicate that G. catenatum prefers P and Se for proliferation, rather than other nutritional sources.

To understand the environmental factors affecting the spatiotemporal distribution of copepods, sampling was conducted seasonally in a small mesotidal inlet and estuary located in Doam Bay of southwestern Korea. The study area was divided seasonally into two or three station groups (estuarine, mixed, and coastal) by a cluster analysis and non-metric multidimensional scaling based on copepod abundance. Acartia forticrusa, A. hudsonica, A. ohtsukai, Paracalanus parvus s. l., Pseudodiaptomus marinus, Tortanus derjugini, T. dextrilobatus, T. forcipatus, Oithona spp., and harpacticoids were important species for grouping the stations. The spatiotemporal distribution of the first two species was restricted to the estuarine area in summer and significantly correlated with temperature, salinity, and chlorophyll-a concentration. The distribution of other brackish species, such as T. derjugini and T. dextrilobatus, significantly correlated with temperature, salinity, and chlorophyll-a concentration. In contrast, A. hudsonica significantly correlated with dinoflagellate density and turbidity in winter, in addition to the abovementioned environmental factors. Acartia hudsonica also maintained a large population in the estuarine area in fall and winter, and its distribution extended across the entire bay in spring. Other coastal species occurred in all areas and did not significantly correlate with environmental factors. Therefore, brackish species in the study area may have developed seasonally different behaviors to sustain their populations.

The dinoflagellate genus Scrippsiella is a common member of phytoplankton and their cysts are also frequently reported in coastal sediments worldwide. However, the diversity of Scrippsiella in Korean waters has not been fully investigated. Here, several isolates of Scrippsiella precaria collected from Korean waters and germinated from resting cysts were examined using light and scanning electron microscopy. The resting cysts were characterized by pointed calcareous spines and one or two red accumulation bodies, and the archeopyle was mesoepicystal, representing the loss of 2–4′ and 1–3a paraplates. Rounded resting cysts were found in culture, and an increase in spine length was observed until 8 days of development. Korean isolates of S. precaria had the plate formula of Po, X, 4′, 3a, 7″, 6C, 4S, 5‴, 2⁗. There were differences in the cell size and location of the red body between Korean isolates and previously described cells of S. precaria. In addition, the Korean isolates of S. precaria had two types of the 5″ plate that either contacted the 2a plate or not. Molecular phylogeny based on internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequences revealed that the Korean isolates were nested within the subclade of PRE (S. precaria and related species) in the clade of Scrippsiella sensu lato, and that the PRE subclade had two ribotypes: ribotype 1 consisting of the isolates from Korea, China, and Australia, and ribotype 2 consisting of the isolates from Italy and Greece. Lineages between isolates of ribotype 1 were likely to be related to the dispersal by ocean currents and ballast waters from international shipping, and the two types of spine shapes and locations of the 5″ plates may be a distinct feature for ribotype 1.

This study investigated the tolerance of resting eggs of Eurytemora pacifica to storage under low temperatures, which is of particular interest in light of the recent use of nauplii as living food in aquaculture, other than conditions experienced also in the wild during winter cold events in the Northern Hemisphere. Sediment samples collected in August 2020 were used to store the resting eggs at two different freezing temperatures (−5 and −20 °C) for five different durations (1, 3, 6, 9, and 12 months). The mean hatching success rates of the resting eggs after one month of storage were 85.3 ± 1.5% (−5 °C) and 85.0 ± 3.6% (−20 °C), with no significant difference between freezing temperatures. However, significant differences emerged over time, with the mean hatching success rate remaining at 85.0 ± 3.6% at −5 °C after three months, while it dropped sharply to 1.7 ± 2.1% at −20 °C. For the non-freezing conditions, the hatching success at 10 °C increased gradually over the one-month incubation period, ultimately reaching 71.0%. These findings demonstrate the remarkable cold tolerance of E. pacifica but also indicate a limit to this tolerance at longer durations. These results underscore the importance of considering the adoption of storage freezing for resting eggs to be used for aquaculture and also suggest the possibility of the species better surviving the extreme weather events in comparison with other species.