Biocrusts are associations of various prokaryotic and eukaryotic microorganisms in the top millimeters of soil, which can be found in every climate zone on Earth. They stabilize soils and introduce carbon and nitrogen into this compartment. The worldwide occurrence of biocrusts was proven by numerous studies in Europe, Africa, Asia and North America, leaving South America understudied. Using an integrative approach, which combines morphological and molecular characters (small subunit rRNA and ITS region), we examined the diversity of key biocrust photosynthetic organisms at four sites along the latitudinal climate gradient in Chile. The most northern study site was located in the Atacama Desert (arid climate), followed by open shrubland (semiarid climate), a dry forest region (Mediterranean climate) and a mixed broad leaved-coniferous forest (temperate climate) in the south. The lowest species richness was recorded in the desert (18 species), whereas the highest species richness was observed in the Mediterranean zone (40 species). Desert biocrusts were composed exclusively of single-celled Chlorophyta algae, followed by cyanobacteria. Chlorophyta, Streptophyta and cyanobacteria dominated semiarid biocrusts, whereas Mediterranean and temperate Chilean biocrusts were composed mostly of Chlorophyta, Streptophyta and Ochrophyta. Our investigation of Chilean biocrust suggests high biodiversity of South American biocrust phototrophs.

Most of the data on biodiversity, ecology and biogeography of microalgae and cyanobacteria are based on classical morphological approaches. However, morphological identification of these microorganisms is often possible only to higher taxonomic ranks such as genus, because of low morphological diversity and high variability along with the presence of cryptic taxa. This study compared the species composition of cyanobacteria, and green and streptophyte microalgae, isolated from biological soil crusts (BSCs) of maritime sand dunes (Baltic Sea, Germany) and identified through culture-dependent morphological methods combined with molecular phylogenetic data. Phylogenetic analyses (based on SSU rRNA and ITS-1,2 for microalgae, or the SSU-LSU ITS region for cyanobacteria) provided unambiguous identifications of 20 strains (37.7% of all strains investigated) to species level; the identifications had to be corrected after preliminary morphological determination of eight strains. For 27 strains (50.9%), identification with molecular markers was possible only to genus or group of closely related species, because a modern taxonomic revision of these taxa is preliminary, incomplete or non-existent. Identification to genus was problematic for six strains (11.3%). This combination of morphological and molecular methods (integrative approach) resulted in the discovery of rare, taxonomically or ecologically interesting taxa, and in the description of a new species of Tetradesmus (T. arenicola sp. nov.), a new combination for Chlamydomonas callunae (Heterochlamydomonas callunae comb. nov.), epitypification of the rare alga Actinochloris sphaerica and emendation of the recently described Eremochloris sphaerica. A microscopic culture-dependent method and an integrative approach provided similar results concerning the cyanobacterial and algal diversity of BSCs of Baltic sand dunes in general. However, this investigation using an integrative approach allowed us to precisely identify species of several important taxa (the most common and dominant species) in the community structure of BSCs, and also certain rare representatives.

The polyphasic approach has been widely applied in cyanobacterial taxonomy, which frequently led to additions to the species inventory. Increasing our knowledge about species and the habitats they were isolated from enables new insights into the ecology of newly established genera and species allowing speculations about the ecological niche of taxa. Here, we are describing three new species belonging to three genera that broadens the ecological amplitude and the geographical range of each of the three genera. Cyanocohniella crotaloides sp. nov. is described from sandy beach mats of the temperate island Schiermonnikoog, Netherlands, Oculatella crustae‐formantes sp. nov. was isolated from biological soil crusts of the Arctic Spitsbergen, Norway, and Aliterella chasmolithica originated from granitic stones of the arid Atacama Desert, Chile. All three species could be separated from related species using molecular sequencing of the 16S rRNA gene and 16S–23S ITS gene region, the resulting secondary structures as well as p‐distance analyses of the 16S–23S ITS and various microscopic techniques. The novel taxa described in this study contribute to a better understanding of the diversity of the genera Cyanocohniella, Oculatella, and Aliterella in different habitats.

Two new genera (Streptosarcina and Streptofilum) and three new species (Streptosarcina arenaria, S. costaricana and Streptofilum capillatum) of streptophyte algae were detected in cultures isolated from terrestrial habitats of Europe and Central America and described using an integrative approach. Additionally, a strain isolated from soil in North America was identified as Hormidiella parvula and proposed as an epitype of this species. The molecular phylogeny based on 18S rRNA and rbcL genes, secondary structure of ITS-2, as well as the morphology of vegetative and reproductive stages, cell ultrastructure, ecology and distribution of the investigated strains were assessed. The new genus Streptosarcina forms a sister lineage to the genus Hormidiella (Klebsormidiophyceae). Streptosarcina is characterized by packet-like (sarcinoid) and filamentous thalli with true branching and a cell organization typical for Klebsormidiophyceae. Streptofilum forms a separate lineage within Streptophyta. This genus represents an easily disintegrating filamentous alga which exhibits a cell coverage of unique structure: layers of submicroscopic scales of piliform shape covering the plasmalemma and exfoliate inside the mucilage envelope surrounding cells. The implications of the discovery of the new taxa for understanding evolutionary tendencies in the Streptophyta, a group of great evolutionary interest, are discussed.

Representatives of the closely related genera, Interfilum and Klebsormidium, are characterized by unicells, dyads or packets in Interfilum and contrasting uniseriate filaments in Klebsormidium. According to the literature, these distinct thallus forms originate by different types of cell division, sporulation (cytogony) versus vegetative cell division (cytotomy), but investigations of their morphology and ultrastructure show a high degree of similarity. Cell walls of both genera are characterized by triangular spaces between cell walls of neighbouring cells and the parental wall or central space among the walls of a cell packet, exfoliations and projections of the parental wall and cap-like and H-like fragments of the cell wall. In both genera, each cell has its individual cell wall and it also has part of the common parental wall or its remnants. Therefore, vegetative cells of Interfilum and Klebsormidium probably divide by the same type of cell division (sporulation-like). Various strains representing different species of the two genera are characterized by differences in cell wall ultrastructure, particularly the level of preservation, rupture or gelatinization of the parental wall surrounding the daughter cells. The differing morphologies of representatives of various lineages result from features of the parental wall during cell separation and detachment. Cell division in three planes (usual in Interfilum and a rare event in Klebsormidium) takes place in spherical or short cylindrical cells, with the chloroplast positioned perpendicularly or obliquely to the filament (dyad) axis. The morphological differences are mainly a consequence of differing fates of the parental wall after cell division and detachment. The development of different morphologies within the two genera mostly depends on characters such as the shape of cells, texture of cell walls, mechanical interactions between cells and the influence of environmental conditions.

Potash tailing piles caused by fertilizer production shape their surroundings because of the associated salt impact. A previous study in these environments addressed the functional community “biocrust” comprising various micro- and macro-organisms inhabiting the soil surface. In that previous study, biocrust microalgae and cyanobacteria were isolated and morphologically identified amongst an ecological discussion. However, morphological species identification maybe is difficult because of phenotypic plasticity, which might lead to misidentifications. The present study revisited the earlier species list using an integrative approach, including molecular methods. Seventy-six strains were sequenced using the markers small subunit (SSU) rRNA gene and internal transcribed spacer (ITS). Phylogenetic analyses confirmed some morphologically identified species. However, several other strains could only be identified at the genus level. This indicates a high proportion of possibly unknown taxa, underlined by the low congruence of the previous morphological identifications to our results. In general, the integrative approach resulted in more precise species identifications and should be considered as an extension of the previous morphological species list. The majority of taxa found were common in saline habitats, whereas some were more likely to occur in nonsaline environments. Consequently, biocrusts in saline environments of potash tailing piles contain unique microalgae and cyanobacteria that will possibly reveal several new taxa in more detailed future studies and, hence, provide new data on the biodiversity, as well as new candidates for applied research.