Background and Objective Dan-Hong injection, which comprises extracts of Salvia miltiorrhiza and Carthamus tinctorius, promotes blood circulation and reduces blood stasis. Combination of S. miltiorrhiza and C. tinctorius is more effective in treating cerebral ischemia than S. miltiorrhiza alone. This study aimed to examine the pharmacokinetic characteristics of four active ingredients of S. miltiorrhiza and C. tinctorius, namely danshensu (DSS), hydroxysafflor yellow A (HSYA), and salvianolic acid A (SAA) and B (SAB) in normal and cerebral ischemia rats. Methods Normal and cerebral ischemia rats were injected via the tail vein with each active ingredient, and blood was collected through the jaw vein at different time points. The plasma concentration of the compatibility group was analyzed by high-performance liquid chromatography, and pharmacokinetic parameters were determined using Pharmacokinetic Kinetica 4.4 software. Results The pharmacokinetics of the four active ingredients in the normal and cerebral ischemia rats were consistent with a two-compartment model. The area under the concentration–time curve was higher in normal rats than in cerebral ischemia rats, with a highly significant difference for SAA (P < 0.01). Clearance rates were lower in normal rats than in cerebral ischemia rats, with DSS showing the most significant difference (P < 0.01). Furthermore, there were significant differences between normal and cerebral ischemia rats in the distribution phase-elimination half life for DSS, SAA, and HSYA, as well as in the apparent volume of distribution for the central compartment for DSS and HSYA (P < 0.01). The plasma concentrations of the four active ingredients were higher in normal rats than in cerebral ischemia rats. Conclusion Cerebral ischemia rats showed higher drug clearance rates and longer retention times than normal rats, which may be due to destruction of the blood–brain barrier during cerebral ischemia–reperfusion. The four active ingredients likely integrated and interacted with each other to affect target sites in the brain to protect against cerebral ischemic injury.

Ezrin is an actin binding protein connecting the cell membrane and the cytoskeleton, which is crucial to maintaining cell morphology, intercellular adhesion, and cytoskeleton remodeling. Asthma involves dysfunction of inflammatory cells, cytokines, and airway structural cells. Recent studies have shown that ezrin, whose function is affected by extensive phosphorylation and protein interactions, is closely associated with asthma, may be a therapeutic target for asthma treatment. In this review, we summarize studies on ezrin and discuss its role in asthma-related airway inflammation and remodeling.

Introduction: An objective and precise pain evaluation is of significant clinical value, and electroencephalography as a non-invasive physiological signal has been demonstrated to correlate with subjective pain perception. This study aimed to analyze the EEG changes in patients with lumbar disk herniation (LDH) under traditional Chinese medicine small needle knife and to further explore the feasibility of EEG as an indicator of pain assessment in patients with LDH.Methods: This study conducted resting-state electroencephalography on 20 patients with LDH before and after treatment and on 20 healthy controls, respectively. Following the spectral analysis of the EEG signals with continuous wavelet transform, power ratios were extracted for four frequency bands (θ, α, β and γ). Significance tests were conducted within the LDH group and between the LDH and healthy controls, as well as correlation analyses of EEG characteristics with pain scales in four regions of interest.Results: A significant reduction in subjective pain intensity was observed after small needle knife, with a 32.86 and 38.41% reduction in the Visual Analog Scale (VAS) and modified Japanese Orthopedic Association (mJOA) scores, respectively. Alpha accounted for a significantly higher of the four regions, while theta in the frontal, occipital and beta in the central were significantly lower. HC had fewer EEG oscillations in the theta band compared to LDH. The constructed alpha/beta features demonstrated a significant negative correlation with VAS in the frontal (R = −0.361, P = 0.022) and parietal (R = −0.341, P = 0.031), as well as with mJOA in the frontal (R = −0.416, P = 0.007), central (R = −0.438, P = 0.004), and parietal (R = −0.390, P = 0.013) regions.Conclusion: EEG power ratios showed significantly different results in LDH groups, and between patients and HC. The alpha/beta features of the frontal and parietal constructed in this study showed correlations with subjective pain scores and might serve as a biomarker of pain status in the short term in LDH.

Allergic asthma is an inflammatory disease involving the Th1/Th2 cell imbalance in the peripheral blood. Repeated herbal acupoint sticking (RHAS) has been used for hundreds of years in China to relieve the recurrence of allergic asthma, and it is still practiced today. Thus, we explored the effect on allergic asthma relapse and the underlying immunoregulatory mechanism in this study. Here, we enrolled 50 allergic asthma participants, and 38 of them completed the treatment and follow‐up (the allergic asthma group). In addition, 13 healthy participants (the control group) were enrolled. The recurrence number of allergic asthma participants and asthma control test (ACT) were used to evaluate the effect of treatment on relieving allergic asthma recurrence. Flow cytometry was performed to analyze the levels of Th1 and Th2 cells in the peripheral blood. The serum levels of IgE, IFN‐γ, and IL‐4 were detected by ELISA. (1) In the allergic asthma group, compared to before the first treatment, the recurrence number of allergic asthma participants decreased and the ACT score increased at end of the last treatment, 18 and 30 weeks of the trial (P < 0.05). At 18 and 30 weeks of the trial, the recurrence number of allergic asthma participants was less and the ACT score was higher than the ones from the same period last year in the allergic asthma group (P < 0.05). Compared to before the first treatment, the percentage of Th1 cell did not change significantly, the percentage of Th2 cell decreased, and the Th1/Th2 cell ratio increased in the allergic asthma group by the end of the last treatment (P < 0.05). Meanwhile, the release of IgE and IL‐4 reduced (P < 0.05), and the release of IFN‐γ did not significantly change in the allergic asthma group. (2) Compared with the control group, the serum levels of IgE and IL‐4 and the percentage of Th2 cell were higher, and the Th1/Th2 cell ratio was lower in the allergic asthma group (P < 0.05). There was no significant difference between Th1 cell and IFN‐γ before the first treatment. (3) Compared with the control group, the IgE levels and the percentage of Th2 cell were higher in the allergic asthma group (P < 0.01). Simultaneously, there was no significant difference between Th1 cell, the Th1/Th2 cell ratio, and the serum levels of IFN‐γ and IL‐4 by the end of the last treatment. The data suggested that RHAS reduced the amount of Th2 cell and elevated the Th1/Th2 cell ratio, thereby alleviating the inflammatory responses in the allergic asthma participants.