In this study, we customized magnetic sorbents by functionalizing silica coated magnetite with octadecyl(C₁₈)silane (Fe₃O₄@SiO₂@C₁₈). This sorbent was intended for the determination of trace orthophosphate (o-PO₄³⁻) in unpolluted freshwater samples. The o-PO₄³⁻ was transformed to phosphomolybdenum blue (PMB), a known polyoxometalate ion. Then the PMB were coupled with cetyl trimethyl ammonium bromide (CTAB), cationic surfactant, in order to hydrophobically bound with the Fe₃O₄@SiO₂@C₁₈ particles through dispersive magnetic solid-phase extraction (d-MSPE) as part of sample preconcentration. The PMB–CTAB–magnetic particles are simply separated from the aqueous solution by the external magnet. The acidified ethanol 0.5 mL was used as PMB-CTAB eluent to produce an intense blue solution, which the absorbance was measured using a UV–Vis spectrophotometer at 800 nm. The proposed method (employing 2 mg of Fe₃O₄@SiO₂@C₁₈) yielded an enhancement factor of 32 with a linear range of 1.0–30.0 µg P L⁻¹. Precision at 6.0 µg P L⁻¹ and 25.0 µg P L⁻¹ were 3.70 and 2.49% (RSD, n = 6) respectively. The lower detection limit of 0.3 µg P L⁻¹ and quantification limit of 1.0 µg P L⁻¹ allowed trace levels analysis of o-PO₄³⁻ in samples. The reliability and accuracy of the proposed method were confirmed by using a certified reference material. Our method offers highly sensitive detection of o-PO₄³⁻ with simple procedures that can be operated at room temperature and short analysis time.

Improvement of nutritional value and reduction of antinutritional factors (ANFs) of soybean meal (SBM) for animal feed applications could be achieved by using bromelain immobilized onto bentonite (Bt)-carboxymethylcellulose (CMC) composites. The composite with mass ratio between CMC to calcium ion (Ca²⁺) at 1:20 provided the highest enzyme activity, immobilization yield higher than 95%, with superior thermal and storage stabilities. Performance of the immobilized bromelain for soybean protein hydrolysis was further studied. The results showed that at 60 °C, the immobilized bromelain exhibited the highest efficiency in enzymatic hydrolysis to release free alpha amino nitrogen (FAN) as a product with high selectivity and to effectively reduce SBM allergenic proteins within 30 min. In conclusion, immobilization of bromelain onto Bt-CMC composites leads to stability enhancement of the enzyme, enabling effective improvement in SBM quality in a short treatment time and showing great potential for application in animal feed industries.

Cobaltite oxide spinel (CoCo₂O₄) is one promising material that has been extensively studied for decades due to its versatile applications. Revealing the correlation among chemical compositions, cationic distributions, and physical properties are crucial for exploring its novel application. Here, a series of nickel/zinc co-substituted cobaltite spinels, Zn_1−XNi_XCo₂O₄ (ZNCO-X; where X = 0.00, 0.25, …, 1.00), was synthesized by calcining the hydrothermal-derived precursors and their magnetic properties have been investigated. Multiple x-ray based characterization techniques (XRD, XRF, XPS, and XAS) were applied to determine the crystalline structure and appropriated compositions of cation species (Zn²⁺, Ni²⁺, Ni³⁺, Co²⁺, and Co³⁺). In conjunction with Neel’s theory of antiferromagnetism, the theoretical magnetization of the spinel series was calculated based on the assumption that Zn²⁺ ion was located in tetrahedral (A site) while nickel cations (Ni²⁺ and Ni³⁺) occupying the octahedral (B site). The theoretical magnetization profile exhibited a good correlation. Superparamagnetic effect and cationic site exchange can be used to explain the discrepancies between the measured and calculated magnetizations. This work reported a systematic controlling of materials structure and cationic distribution, which are crucial for fine-tuning the magnetic property of the Zn_1−XNi_XCo₂O₄ cobaltite system.