Aquaculture uses large volumes of water, which is generally discharged without treatment, possibly causing scarcity and contamination. A sustainable aquaculture option is biofloc technology (BFT), which recycles food residues and toxic organic and inorganic compounds from the system through microorganisms, avoiding excessive use of water and serving as natural food for cultured aquatic organisms. The aim of this study was to identify the main factors that limit a Mexican aquaculture producer from adopting biofloc technology in their aquaculture production units (APUs). Strengths and weaknesses were methodologically analyzed through 248 questionnaires, applied to fish farmers in 16 states of the country with a mixed approach (quantitative and qualitative). Findings reveal that the main obstacles in the use of BFT are due to the following: low academic level, limited administrative capacity, scarce technological equipment in facilities, diversified productive activity, and obsolete regulations. Other factors that promote the adoption of BTFs for aquaculturists are production experience, favorable weather conditions, and abundant availability of water and energy. In conclusion, the use of BTF is a sustainable option for APUs despite the limiting factors identified in this research which slow down the growth of the sector. It is advisable to study Mexican producers with BFT, in order to spread their benefits to other APUs, and further evaluate the productivity of the aquaculture sector. This study considers production aspects, and also sustainable use of its resources, specifically, surface, energy, water, and food.