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Every year, the size of the human population grows; with it, the need for agricultural products increases. This leads to an increment in the volume of waste, including hard-to-degrade keratin-rich ones, such as feathers. Currently, most of the agro-industrial complex protein by-products are utilized by incineration, landfilling, and chemical hydrolysis. Such methods do not meet modern trends in the development of a sustainable economy, negatively affecting the environment and humans, and preventing the reusing of waste. An alternative is biodegradation, which consists of the application of living organisms and their enzymes to recycle by-products. This approach is not only sustainable, but also makes it possible to obtain products of waste hydrolysis that are in demand for the manufacture of fertilizers and feed additives. This brings the development of agriculture closer to a circular economy and makes the recycling process more profitable. This review article emphasizes the significance of keratinolytic microorganisms and keratinases for the improvement of green methods for processing hard-to-degrade protein waste of the agro-industrial complex, which is necessary for sustainable economic development.
Anna Shestakova; Svetlana Timorshina; Alexander Osmolovskiy. Biodegradation of Keratin-Rich Husbandry Waste as a Path to Sustainable Agriculture. Sustainability 2021, 13, 8691 .
AMA StyleAnna Shestakova, Svetlana Timorshina, Alexander Osmolovskiy. Biodegradation of Keratin-Rich Husbandry Waste as a Path to Sustainable Agriculture. Sustainability. 2021; 13 (16):8691.
Chicago/Turabian StyleAnna Shestakova; Svetlana Timorshina; Alexander Osmolovskiy. 2021. "Biodegradation of Keratin-Rich Husbandry Waste as a Path to Sustainable Agriculture." Sustainability 13, no. 16: 8691.
In this study, we investigated the properties of proteolytic enzymes of two species of Aspergillus, Aspergillus flavus 1 (with a high degree of pathogenicity) and Aspergillus ochraceus L-1 (a conditional pathogen), and their effects on various components of the hemostasis system (in vitro) in the case of their penetration into the bloodstream. We showed that micromycete proteases were highly active in cleaving both globular (albuminolysis) and fibrillar (fibrin) proteins, and, to varying degrees, they could coagulate the plasma of humans and animals (due to proteolysis of factors of the blood coagulation cascade) but were not able to coagulate fibrinogen. The proteases of both Aspergillus fully hydrolyzed thrombi in 120–180 min. Micromycetes did not show hemolytic activity but were able to break down hemoglobin.
Alexander Osmolovskiy; Laura Schmidt; Anastasia Orekhova; Sergey Komarevtsev; Valeriana Kreyer; Sergey Shabunin; Nikolay Egorov. Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins. Life 2021, 11, 782 .
AMA StyleAlexander Osmolovskiy, Laura Schmidt, Anastasia Orekhova, Sergey Komarevtsev, Valeriana Kreyer, Sergey Shabunin, Nikolay Egorov. Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins. Life. 2021; 11 (8):782.
Chicago/Turabian StyleAlexander Osmolovskiy; Laura Schmidt; Anastasia Orekhova; Sergey Komarevtsev; Valeriana Kreyer; Sergey Shabunin; Nikolay Egorov. 2021. "Action of Extracellular Proteases of Aspergillus flavus and Aspergillus ochraceus Micromycetes on Plasma Hemostasis Proteins." Life 11, no. 8: 782.
The best known polymers (alginate, carrageenan, and acrylamide) were examined for their potential use as carriers of mycelium cells of Aspergillus ochraceus VKM-F4104D, a producer of fibrinolytic protease activator of protein C in human blood plasma during its deep cultivation. The morpho-cytological features of the development of A. ochraceus VKM-F4104D mycelium in granules of carrageenan and calcium alginate are shown. The conditions for the cultivation of micromycete immobilized in granules of 3.5% calcium alginate were optimized. The immobilized cells of the producer mycelium demonstrated increased stability and were able to synthesize protein-C protease-activator in blood plasma at the maximum level for 10 or more days. In addition, immobilized mycelial cells during cultivation can be used repeatedly (up to five cycles).
S. K. Komarevtsev; S. N. Timorshina; M. R. Leontieva; S. V. Shabunin; E. S. Lobakova; A. A. Osmolovskiy. Effect of Immobilization of the Micromycete Aspergillus ochraceus VKM-F4104D in Polymeric Carriers on the Production of the Fibrinolytic Protease Activator of Blood Plasma Protein C. Applied Biochemistry and Microbiology 2021, 57, 475 -480.
AMA StyleS. K. Komarevtsev, S. N. Timorshina, M. R. Leontieva, S. V. Shabunin, E. S. Lobakova, A. A. Osmolovskiy. Effect of Immobilization of the Micromycete Aspergillus ochraceus VKM-F4104D in Polymeric Carriers on the Production of the Fibrinolytic Protease Activator of Blood Plasma Protein C. Applied Biochemistry and Microbiology. 2021; 57 (4):475-480.
Chicago/Turabian StyleS. K. Komarevtsev; S. N. Timorshina; M. R. Leontieva; S. V. Shabunin; E. S. Lobakova; A. A. Osmolovskiy. 2021. "Effect of Immobilization of the Micromycete Aspergillus ochraceus VKM-F4104D in Polymeric Carriers on the Production of the Fibrinolytic Protease Activator of Blood Plasma Protein C." Applied Biochemistry and Microbiology 57, no. 4: 475-480.
A new method has been developed to increase the productivity of aspergilli - producers of extracellular proteinases based on their cultivation on vermiculite under solid-state fermentation conditions. The productivity of the mycelium Aspergillus ochraceus L-1 and Aspergillus ustus 1 was 3-18 times higher not only in comparison with submerged cultivation, but also in comparison with growth on other carriers studied under solid-state fermentation conditions. Vermiculite can be considered as a new promising carrier for solid-state fermentation of micromycetes.
Alexander A. Osmolovskiy; Elizaveta A. Popova; Valeriana G. Kreyer; Nina A. Baranova; Nikolay S. Egorov. Vermiculite as a new carrier for extracellular protease production by Aspergillus spp. under solid-state fermentation. Biotechnology Reports 2020, 29, e00576 .
AMA StyleAlexander A. Osmolovskiy, Elizaveta A. Popova, Valeriana G. Kreyer, Nina A. Baranova, Nikolay S. Egorov. Vermiculite as a new carrier for extracellular protease production by Aspergillus spp. under solid-state fermentation. Biotechnology Reports. 2020; 29 ():e00576.
Chicago/Turabian StyleAlexander A. Osmolovskiy; Elizaveta A. Popova; Valeriana G. Kreyer; Nina A. Baranova; Nikolay S. Egorov. 2020. "Vermiculite as a new carrier for extracellular protease production by Aspergillus spp. under solid-state fermentation." Biotechnology Reports 29, no. : e00576.
Proteolytic activity of 8 strains of micromycetes (belonging to different ecological and trophic groups: phyto-, nemato-, myco-, and entomopathogens as well as human pathogens) toward the hemostatic system proteins has been assayed by using the chromogenic peptide substrates. The investigated micromycetes are shown to secrete proteases with a broad substrate specificity. However, there are producers among them exhibiting a pronounced plasmin-like and urokinase activity (Arthrobotrys longa 1, Sarocladium strictum 203) as well as a tPA (tissue plasminogen activator)-like activity (Purpureocillium lilacinum k1). It is found that the most active producer of proteases with fibrinolytic and plasminogen activator activity is S. strictum 203. Plasminogen activator activity of proteinases produced by S. strictum 203 is approximately 50% of the fibrinolytic one, which makes this strain a promising producer of fibrinolytic enzymes.
A. A. Lukianova; E. I. Kornienko; P. A. Vigand; V. G. Kreyer; A. V. Kurakov; A. A. Osmolovskiy. Secretion by Micromycetes of Proteases with Activity Similar to the Activity of the Hemostatic System Proteins. Moscow University Biological Sciences Bulletin 2020, 75, 31 -35.
AMA StyleA. A. Lukianova, E. I. Kornienko, P. A. Vigand, V. G. Kreyer, A. V. Kurakov, A. A. Osmolovskiy. Secretion by Micromycetes of Proteases with Activity Similar to the Activity of the Hemostatic System Proteins. Moscow University Biological Sciences Bulletin. 2020; 75 (1):31-35.
Chicago/Turabian StyleA. A. Lukianova; E. I. Kornienko; P. A. Vigand; V. G. Kreyer; A. V. Kurakov; A. A. Osmolovskiy. 2020. "Secretion by Micromycetes of Proteases with Activity Similar to the Activity of the Hemostatic System Proteins." Moscow University Biological Sciences Bulletin 75, no. 1: 31-35.
It has been shown that factor X activity determined in normal plasma using Aspergillus ochraceus protease is comparable with the activity determined with a commercial analogue, a protease from Russell’s viper venom (RVV-X®). It was revealed that the protease of A. ochraceus, along with the RVV-X® preparation, can be used to determine the content of factor X in the plasma with its reduced content. A study of the protease activity of A. ochraceus as an activator to factor X showed that it is slightly higher compared to the snake venom preparation, which can make A. ochraceus protease a promising substitute for the snake activator in diagnostics for determining the content of factor X.
A. V. Orekhova; A. A. Osmolovskiy; V. G. Kreyer; N. A. Baranova; N. S. Egorov. Possibility for Application of Extracellular Protease of Micromycete Aspergillus ochraceus for Determining Factor X Content in Human Blood Plasma. Moscow University Biological Sciences Bulletin 2019, 74, 117 -120.
AMA StyleA. V. Orekhova, A. A. Osmolovskiy, V. G. Kreyer, N. A. Baranova, N. S. Egorov. Possibility for Application of Extracellular Protease of Micromycete Aspergillus ochraceus for Determining Factor X Content in Human Blood Plasma. Moscow University Biological Sciences Bulletin. 2019; 74 (2):117-120.
Chicago/Turabian StyleA. V. Orekhova; A. A. Osmolovskiy; V. G. Kreyer; N. A. Baranova; N. S. Egorov. 2019. "Possibility for Application of Extracellular Protease of Micromycete Aspergillus ochraceus for Determining Factor X Content in Human Blood Plasma." Moscow University Biological Sciences Bulletin 74, no. 2: 117-120.
A scheme for screening of micromycetes - producers of proteases with the activity of hemostasis system proteins, based on their enzymatic indices determination and the activity towards chromogenic peptide substrates for proteins of the hemostasis system was developed. Depending on the ability of proteases producers to cleave such substrates, an enzymatic reaction in conditions containing human plasma is suggested, which makes it possible to identify the potentiality of the target plasma hemostasis proenzymes activation.
Alexander A. Osmolovskiy; Anna A. Lukianova; Elena S. Zvonareva; Valeriana G. Kreyer; Nina A. Baranova; Nikolay S. Egorov. Combined microbiological approach to screening of producers of proteases with hemostasis system proteins activity among micromycetes. Biotechnology Reports 2018, 19, e00265 -e00265.
AMA StyleAlexander A. Osmolovskiy, Anna A. Lukianova, Elena S. Zvonareva, Valeriana G. Kreyer, Nina A. Baranova, Nikolay S. Egorov. Combined microbiological approach to screening of producers of proteases with hemostasis system proteins activity among micromycetes. Biotechnology Reports. 2018; 19 ():e00265-e00265.
Chicago/Turabian StyleAlexander A. Osmolovskiy; Anna A. Lukianova; Elena S. Zvonareva; Valeriana G. Kreyer; Nina A. Baranova; Nikolay S. Egorov. 2018. "Combined microbiological approach to screening of producers of proteases with hemostasis system proteins activity among micromycetes." Biotechnology Reports 19, no. : e00265-e00265.
The effect of nitrogen sources in the fermentation medium and the cultivation conditions on the production of proteinases with plasmin-like and prekallikrein activation activity by micromycete Aspergillus terreus 2 was investigated. The highest secretion of proteinases was achieved when the micromycete was cultivated on a medium containing both amine- and mineral nitrogen sources at an initial pH of 5.5 and at 28°С. It was established that the extracellular micromycete proteinases are equally capable of hydrolyzing fibrin and fibrinogen.
E. S. Zvonareva; A. A. Osmolovskiy; V. G. Kreier; N. A. Baranova; I. B. Kotova; N. S. Egorov. Production of Proteinase with Plasmin-Like and Prekallikrein Activating Activity by the Micromycete Aspergillus terreus. Applied Biochemistry and Microbiology 2018, 54, 206 -210.
AMA StyleE. S. Zvonareva, A. A. Osmolovskiy, V. G. Kreier, N. A. Baranova, I. B. Kotova, N. S. Egorov. Production of Proteinase with Plasmin-Like and Prekallikrein Activating Activity by the Micromycete Aspergillus terreus. Applied Biochemistry and Microbiology. 2018; 54 (2):206-210.
Chicago/Turabian StyleE. S. Zvonareva; A. A. Osmolovskiy; V. G. Kreier; N. A. Baranova; I. B. Kotova; N. S. Egorov. 2018. "Production of Proteinase with Plasmin-Like and Prekallikrein Activating Activity by the Micromycete Aspergillus terreus." Applied Biochemistry and Microbiology 54, no. 2: 206-210.
Proteolytic activity of extracellular enzymes of 11 strains of different Aspergillus species was studied. Comparison of the enzymatic indices of strains grown on agar medium containing either casein or fibrin allowed the selection of the strain Aspergillus terreus 2 as a promising producer of fibrinolytic proteases. It was found that A. terreus 2 proteinases demonstrated maximum activity at pH 8.0. The highest values of fibrinolytic and total proteolytic activities expressed in UTyr (amount of micromoles of tyrosine released from fibrin or casein for 1 min) were 34.0 and 358.3, respectively. Maximum activities were detected when growing the producer on a medium containing only amine nitrogen sources (fish flour hydrolysate and peptone); however, the amount of extracellular protein and the specific fibrinolytic and total proteolytic activities were greater in the medium containing both mineral and amine nitrogen sources (fish flour hydrolysate and sodium nitrate) than in the medium containing only fish flour hydrolysate and peptone as nitrogen sources.
A. A. Osmolovskiy; E. S. Zvonareva; V. G. Kreyer; N. A. Baranova; N. S. Egorov. Secretion of Proteinases with Fibrinolytic Activity by Micromycetes of the Genus Aspergillus. Moscow University Biological Sciences Bulletin 2017, 73, 39 -42.
AMA StyleA. A. Osmolovskiy, E. S. Zvonareva, V. G. Kreyer, N. A. Baranova, N. S. Egorov. Secretion of Proteinases with Fibrinolytic Activity by Micromycetes of the Genus Aspergillus. Moscow University Biological Sciences Bulletin. 2017; 73 (1):39-42.
Chicago/Turabian StyleA. A. Osmolovskiy; E. S. Zvonareva; V. G. Kreyer; N. A. Baranova; N. S. Egorov. 2017. "Secretion of Proteinases with Fibrinolytic Activity by Micromycetes of the Genus Aspergillus." Moscow University Biological Sciences Bulletin 73, no. 1: 39-42.
It has been shown that micromycetes Aspergillus ustus 1 and Tolypocladium inflatum k1 secrete proteolytic enzymes that possess high collagenolytic, fibrinolytic, and elastolytic activity. The activity of proteinases hydrolyzing fibrillar proteins, which was determined by the cleavage of azo-collagen, was 122.6 × 10–3EAzc/mL in A. ustus 1 and 69.7 × 10–3EAzc/mL in T. inflatum k1 (EAzc is the amount of azocollagen cleaved in 1 min (μg). The maximum values of activity were observed during submerged cultivation of A. ustus 1 for 4 days and of T. inflatum k1 for 5 days. It has been shown that the maximum of collagenolytic and general proteolytic activity during the cultivation of A. ustus 1 are time-separated, unlike T. inflatum k1, which, presumably, can simplify the procedure for obtaining proteinases active against fibrillar proteins.
E. A. Popova; D. M. Bednenko; A. A. Osmolovskiy; V. G. Kreyer; I. B. Kotova; N. S. Egorov. Secretion of Extracellular Proteinases Active against Fibrillar Proteins by Micromycetes. Moscow University Biological Sciences Bulletin 2017, 72, 206 -210.
AMA StyleE. A. Popova, D. M. Bednenko, A. A. Osmolovskiy, V. G. Kreyer, I. B. Kotova, N. S. Egorov. Secretion of Extracellular Proteinases Active against Fibrillar Proteins by Micromycetes. Moscow University Biological Sciences Bulletin. 2017; 72 (4):206-210.
Chicago/Turabian StyleE. A. Popova; D. M. Bednenko; A. A. Osmolovskiy; V. G. Kreyer; I. B. Kotova; N. S. Egorov. 2017. "Secretion of Extracellular Proteinases Active against Fibrillar Proteins by Micromycetes." Moscow University Biological Sciences Bulletin 72, no. 4: 206-210.
The properties of two extracellular proteases of Aspergillus ochraceus VKM F-4104D micromycete with plasmin-like activity have been studied. It has been shown that the enzymes differ in pI (5.05 and 6.83) and have similar molecular weights (about 32 and 35 kDa), pH optima (pH 9.0–10.00 at 45°C), and specificities of action on a limited set of chromogenic peptide substrates of trypsin-like proteases. According to inhibitory analysis, both enzymes belong to the serine proteases. Their properties appeared to be similar to those of the protease, protein C activator, which is the main proteolytic enzyme of A. ochraceus VKM F-4104D. Most likely, proteases of this micromyсetes are isoenzymes.
A. A. Osmolovskiy; V. G. Kreier; N. A. Baranova; N. S. Egorov. Properties of extracellular plasmin-like proteases of Aspergillus ochraceus micromycete. Applied Biochemistry and Microbiology 2017, 53, 429 -434.
AMA StyleA. A. Osmolovskiy, V. G. Kreier, N. A. Baranova, N. S. Egorov. Properties of extracellular plasmin-like proteases of Aspergillus ochraceus micromycete. Applied Biochemistry and Microbiology. 2017; 53 (4):429-434.
Chicago/Turabian StyleA. A. Osmolovskiy; V. G. Kreier; N. A. Baranova; N. S. Egorov. 2017. "Properties of extracellular plasmin-like proteases of Aspergillus ochraceus micromycete." Applied Biochemistry and Microbiology 53, no. 4: 429-434.
Fibrinolytic and fibrinogenolytic activity of surface and submerged cultures of a micromycete Aspergillus ochraceus L-1 was studied. Extracellular proteinases produced by A. ochraceus L-1 were found to exhibit specificity against fibrin and fibrinogen and no activity of plasminogen activators. The highest activity was observed in the cultures grown at 28°С and initial pH 7.0. Fibrinolytic activity was shown to be somewhat above 25% of the total plasmin-like activity of A. ochraceus L-1 proteinases.
A. A. Osmolovskiy; E. D. Rukavitsyna; V. G. Kreier; N. A. Baranova; N. S. Egorov. Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus. Microbiology 2017, 86, 512 -516.
AMA StyleA. A. Osmolovskiy, E. D. Rukavitsyna, V. G. Kreier, N. A. Baranova, N. S. Egorov. Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus. Microbiology. 2017; 86 (4):512-516.
Chicago/Turabian StyleA. A. Osmolovskiy; E. D. Rukavitsyna; V. G. Kreier; N. A. Baranova; N. S. Egorov. 2017. "Production of proteinases with fibrinolytic and fibrinogenolytic activity by a micromycete Aspergillus ochraceus." Microbiology 86, no. 4: 512-516.
The effect of extracellular proteinases of A. flavipes A17, A. fumigatus D1, and A. sydowii 1 on proteins of the human haemostasis system was studied. It was shown that A. fumigatus D1 proteinases are able to hydrolyze a wide range of chromogenic peptide substrates of specific human proteinases of the haemostatic system. Proteinases formed by A. flavipes A17 and A. sydowii 1 have a narrow specificity, mainly to thrombin and plasmin substrates. It was first shown that proteinase of A. flavipes A17 is capable to activate protein C and Factor X. Extracellular proteinase produced by A. sydowii 1 has greater fibrinolytic activity as compared with proteinases produced by A. flavipes A17 and A. fumigatus D1.
A. A. Osmolovskiy; A. V. Kurakov; V. G. Kreyer; N. A. Baranova; N. S. Egorov. Ability of extracellular proteinases of micromycetes Aspergillus flavipes, Aspergillus fumigatus, and Aspergillus sydowii to affect proteins of the human haemostatic system. Moscow University Biological Sciences Bulletin 2017, 72, 20 -24.
AMA StyleA. A. Osmolovskiy, A. V. Kurakov, V. G. Kreyer, N. A. Baranova, N. S. Egorov. Ability of extracellular proteinases of micromycetes Aspergillus flavipes, Aspergillus fumigatus, and Aspergillus sydowii to affect proteins of the human haemostatic system. Moscow University Biological Sciences Bulletin. 2017; 72 (1):20-24.
Chicago/Turabian StyleA. A. Osmolovskiy; A. V. Kurakov; V. G. Kreyer; N. A. Baranova; N. S. Egorov. 2017. "Ability of extracellular proteinases of micromycetes Aspergillus flavipes, Aspergillus fumigatus, and Aspergillus sydowii to affect proteins of the human haemostatic system." Moscow University Biological Sciences Bulletin 72, no. 1: 20-24.
The cultivation conditions (cultivation duration, nitrogen sources, and initial pH of the medium) of the strain Tolypocladium inflatum k1, a producer of extracellular proteases, has been optimized to achieve maximal plasminogen activator activity. It has been shown that Tolypocladium inflatum k1 forms at least two proteinases with activator activity towards plasminogen. One of them is thiol-dependent, EDTA-sensitive serine proteinase, which activates plasminogen and does not hydrolyze fibrin, indicating its higher specificity and prospects for the development of new thrombolytic agents.
T. S. Sharkova; E. O. Matveeva; V. G. Kreier; A. A. Osmolovskiy; A. V. Kurakov; N. A. Baranova; N. S. Egorov. Production of proteinase–plasminogen activators by micromycete Tolypocladium inflatum k1. Applied Biochemistry and Microbiology 2016, 52, 31 -35.
AMA StyleT. S. Sharkova, E. O. Matveeva, V. G. Kreier, A. A. Osmolovskiy, A. V. Kurakov, N. A. Baranova, N. S. Egorov. Production of proteinase–plasminogen activators by micromycete Tolypocladium inflatum k1. Applied Biochemistry and Microbiology. 2016; 52 (1):31-35.
Chicago/Turabian StyleT. S. Sharkova; E. O. Matveeva; V. G. Kreier; A. A. Osmolovskiy; A. V. Kurakov; N. A. Baranova; N. S. Egorov. 2016. "Production of proteinase–plasminogen activators by micromycete Tolypocladium inflatum k1." Applied Biochemistry and Microbiology 52, no. 1: 31-35.
It was shown that extracellular proteinases produced by the strains of micromycetes A. ochraceus L-1 and A. ustus 1 differ by the activity at various pH as well as by the intensity of the effect on fibrillar proteins. It was revealed that the proteinases of A. ochraceus L-1 demonstrated maximum activity during the growth of the producer in the nitrate-free growth medium (the pH of enzyme reaction was 8.0), whereas those of A. ustus 1 showed maximal activity during the growth of the micromycete in the medium containing sodium nitrate (the pH of enzyme reaction was 6.0). Values of specific fibrinolytic and collagenolytic activities of A. ochraceus L-1 were 2.2 and 1.6 times higher than those of A. ustus 1. At the same time, A. ustus 1 showed very low values of total proteolytic (caseinolytic) activity and had a high ratio of fibrinolytic activity to total proteolytic (caseinolytic) activity (6.92). It makes the strain a promising producer of proteinases, which hydrolyze fibrin and collagen.
A. A. Osmolovskiy; E. A. Popova; V. G. Kreyer; N. A. Baranova; N. S. Egorov. Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1. Moscow University Biological Sciences Bulletin 2016, 71, 62 -66.
AMA StyleA. A. Osmolovskiy, E. A. Popova, V. G. Kreyer, N. A. Baranova, N. S. Egorov. Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1. Moscow University Biological Sciences Bulletin. 2016; 71 (1):62-66.
Chicago/Turabian StyleA. A. Osmolovskiy; E. A. Popova; V. G. Kreyer; N. A. Baranova; N. S. Egorov. 2016. "Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1." Moscow University Biological Sciences Bulletin 71, no. 1: 62-66.
Differences in the action of extracellular proteases from Aspergillus ochraceus and Aspergillus terreus on proteins of plasma hemostasis which is responsible for the activation initiation of proteins of the prothrombin complex have been revealed. It has been found that proteases produced by A. ochraceus have a direct influence on protein C and factor X while the exoprotease from A. terreus causes their activation indirectly via stimulation of the kallikrein system. The ability of extracellular proteases of micromycetes to activate prekallikrein in human blood plasma has been demonstrated for the first time by the example of A. terreus.
E. S. Zvonareva; A. A. Osmolovskiy; V. G. Kreyer; N. A. Baranova; I. B. Kotova; N. S. Egorov. Identification of Targets for Extracellular Proteases Activating Proteins of the Haemostatic System Produced by Micromycetes Aspergillus ochraceus and Aspergillus terreus. Russian Journal of Bioorganic Chemistry 2015, 41, 500 -505.
AMA StyleE. S. Zvonareva, A. A. Osmolovskiy, V. G. Kreyer, N. A. Baranova, I. B. Kotova, N. S. Egorov. Identification of Targets for Extracellular Proteases Activating Proteins of the Haemostatic System Produced by Micromycetes Aspergillus ochraceus and Aspergillus terreus. Russian Journal of Bioorganic Chemistry. 2015; 41 (5):500-505.
Chicago/Turabian StyleE. S. Zvonareva; A. A. Osmolovskiy; V. G. Kreyer; N. A. Baranova; I. B. Kotova; N. S. Egorov. 2015. "Identification of Targets for Extracellular Proteases Activating Proteins of the Haemostatic System Produced by Micromycetes Aspergillus ochraceus and Aspergillus terreus." Russian Journal of Bioorganic Chemistry 41, no. 5: 500-505.
Screening for producers of proteinases with fibrinolytic (plasmin-like and plasminogen-activating) and collagenolytic activities was carried out among 83 strains of microscopic fungi belonging to various ecological groups. Entomopathogenic micromycetes secreted proteinases with higher fibrinolytic and collagenolytic activity than saprotrophic, potentially phytopathogenic, and epiphytic strains. Micromycete strains possessing proteolytic enzymes with collagenase activity were revealed, as were the strains producing proteinases with plasminlike activity. None of the strains studied secreted proteinases possessing only plasminogen-activating activity. Tolypocladium inflatum k1 was found to be a producer of extracellular proteinases with high plasminogen-activating, plasminlike, and collagenolytic activities. The specific plasminogen-activating activity of T. inflatum k1 was shown to be 20% higher than its plasminlike activity.
T. S. Sharkova; A. V. Kurakov; A. A. Osmolovskiy; E. O. Matveeva; V. G. Kreyer; N. A. Baranova; N. S. Egorov. Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes. Microbiology 2015, 84, 359 -364.
AMA StyleT. S. Sharkova, A. V. Kurakov, A. A. Osmolovskiy, E. O. Matveeva, V. G. Kreyer, N. A. Baranova, N. S. Egorov. Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes. Microbiology. 2015; 84 (3):359-364.
Chicago/Turabian StyleT. S. Sharkova; A. V. Kurakov; A. A. Osmolovskiy; E. O. Matveeva; V. G. Kreyer; N. A. Baranova; N. S. Egorov. 2015. "Screening of producers of proteinases with fibrinolytic and collagenolytic activities among micromycetes." Microbiology 84, no. 3: 359-364.
The properties of an extracellular proteinase activating plasma protein C isolated from the culture supernatant of Aspergillus ochraceus VKM F-4104D have been studied. This enzyme demonstrated a substrate specificity absent of hydrolyzing activity toward chromogenic proteinase substrates. On the basis of inhibitory analysis, the protein C-activating proteinase from A. ochraceus VKM F-4104D appeared to be a serine proteinase-protein C activator, together with that isolated from the venom of Agkistrodon contortrix contortrix. The isolated enzyme was a nonglycosylated protein with a molecular weight of about 33 kDa, pI 6.0 with an observed optimal activity under a pH of 8.0–9.0 and 37°C. A comparison of the properties of the protein C-activating proteinase formed by A. ochraceus and the enzyme derived from the venom of Agk. contortrix contortrix demonstrated a similarity in their properties; however, proteinase from the micromycete appeared to be in the nonglycosylated state and possessed the ability to hydrolyze the chromogenic plasmin substrate H-D-Val-Leu-Lys-pNA.
A. A. Osmolovskiy; V. G. Kreyer; N. A. Baranova; A. V. Kurakov; N. S. Egorov. Properties of extracellular proteinase—an activator of protein C in blood plasma formed by Aspergillus ochraceus. Applied Biochemistry and Microbiology 2014, 51, 95 -101.
AMA StyleA. A. Osmolovskiy, V. G. Kreyer, N. A. Baranova, A. V. Kurakov, N. S. Egorov. Properties of extracellular proteinase—an activator of protein C in blood plasma formed by Aspergillus ochraceus. Applied Biochemistry and Microbiology. 2014; 51 (1):95-101.
Chicago/Turabian StyleA. A. Osmolovskiy; V. G. Kreyer; N. A. Baranova; A. V. Kurakov; N. S. Egorov. 2014. "Properties of extracellular proteinase—an activator of protein C in blood plasma formed by Aspergillus ochraceus." Applied Biochemistry and Microbiology 51, no. 1: 95-101.
Specific features in the development of micromycetes, typical mechanisms of their enzyme production, and conditions providing for an increase in enzyme secretion by the microscopic fungi in solid-state (on natural substrates and inert carriers) and membrane-surface liquid cultures are considered. The prospects and advantages of these fermentation methods for the production of extracellular enzymes are discussed and compared with submerged cultures.
A. A. Osmolovskiy; N. A. Baranova; V. G. Kreier; A. V. Kurakov; N. S. Egorov. Solid-state and membrane-surface liquid cultures of micromycetes: Specific features of their development and enzyme production (a Review). Applied Biochemistry and Microbiology 2014, 50, 219 -227.
AMA StyleA. A. Osmolovskiy, N. A. Baranova, V. G. Kreier, A. V. Kurakov, N. S. Egorov. Solid-state and membrane-surface liquid cultures of micromycetes: Specific features of their development and enzyme production (a Review). Applied Biochemistry and Microbiology. 2014; 50 (3):219-227.
Chicago/Turabian StyleA. A. Osmolovskiy; N. A. Baranova; V. G. Kreier; A. V. Kurakov; N. S. Egorov. 2014. "Solid-state and membrane-surface liquid cultures of micromycetes: Specific features of their development and enzyme production (a Review)." Applied Biochemistry and Microbiology 50, no. 3: 219-227.
The conditions for the submerged and solid-state fermentation of the micromycete Aspergillus ochraceus VKM F-4104D, producing extracellular proteinases that activate protein C of human blood plasma, were optimized. It is shown that the protein C-activating activity of the micromycete in a solid-state culture was 1.5-3.5 times higher than in a submerged culture (as calculated per milliliter of culture medium). Among the extracellular proteins secreted by A. ochraceus VKM F-4104D during submerged and solid-state fermentation, a protein C-activating proteinase with a pI of 6.0–6.3 was identified.
A. A. Osmolovsky; V. G. Kreier; N. A. Baranova; A. V. Kurakov; N. S. Egorov. Production of extracellular proteinases—protein C activators of blood plasma—by the micromycete Aspergillus ochraceus during submerged and solid-state fermentation. Applied Biochemistry and Microbiology 2013, 49, 581 -586.
AMA StyleA. A. Osmolovsky, V. G. Kreier, N. A. Baranova, A. V. Kurakov, N. S. Egorov. Production of extracellular proteinases—protein C activators of blood plasma—by the micromycete Aspergillus ochraceus during submerged and solid-state fermentation. Applied Biochemistry and Microbiology. 2013; 49 (6):581-586.
Chicago/Turabian StyleA. A. Osmolovsky; V. G. Kreier; N. A. Baranova; A. V. Kurakov; N. S. Egorov. 2013. "Production of extracellular proteinases—protein C activators of blood plasma—by the micromycete Aspergillus ochraceus during submerged and solid-state fermentation." Applied Biochemistry and Microbiology 49, no. 6: 581-586.