Understanding the effects of environmental disturbances on insects is crucial in predicting the impact of climate change on their distribution, abundance, and ecology. As microbial symbionts are known to play an integral role in a diversity of functions within the insect host, research examining how organisms adapt to environmental fluctuations should include their associated microbiota. In this study, subterranean termites [Reticulitermes flavipes (Kollar)] were exposed to three different temperature treatments characterized as low (15°C), medium (27°C), and high (35°C). Results suggested that pre-exposure to cold allowed termites to stay active longer in decreasing temperatures but caused termites to freeze at higher temperatures. High temperature exposure had the most deleterious effects on termites with a significant reduction in termite survival as well as reduced ability to withstand cold stress. The microbial community of high temperature exposed termites also showed a reduction in bacterial richness and decreased relative abundance of Spirochaetes, Elusimicrobia, and methanogenic Euryarchaeota. Our results indicate a potential link between gut bacterial symbionts and termite’s physiological response to environmental changes and highlight the need to consider microbial symbionts in studies relating to insect thermosensitivity.

This study evaluated biological resistance, water absorption, and total carbohydrate contents of carbonized woods by contact charring method followed by linseed oil submersion. Charred and control specimens were exposed in laboratory fungal resistance tests to two white – and two brown-rot fungi and in mold resistance tests by spraying the surface with a mold spore-suspension comprised of three different mold species. Charred and control specimens were also evaluated for resistance to the eastern subterranean termites. To help understand biological durability results, carbohydrate content and water absorption of test specimens were also determined. In all cases, durability against wood degrading fungi increased with the exception of charred Eucalyptus bosistoana wood exposing to the white-rot fungus, Irpex lacteus. Mold resistance of charred Pinus taeda specimens increased considerably when compared to controls however, no mold growth was observed on either control or charred specimens of E. bosistoana. In termite tests, significantly less weight losses were shown in charred specimens of both wood species tested compared to controls although, no complete protection was achieved against termite attack. Total carbohydrate content of charred wood specimens decreased after the carbonization process and as expected, increased hydrophobicity was observed with >50% less water uptake in charred specimens.

Eastern subterranean termites, Reticulitermes flavipes (Kollar), are widely distributed across North America where they are exposed to a broad range of environmental conditions. However, mechanisms for overwintering are not well understood. Wisconsin is a unique location to study mechanisms of cold tolerance as it represents the northern boundary for persistent R. flavipes populations. In this study, we evaluated seasonal shifts in cold tolerance using critical thermal minimum (CT_min) and supercooling point (SCP) and examined how these measurements correlate to changes in the microbial community of the termite gut. Results showed seasonal acclimatization to cold, which is consistent with the use of behavioral freeze-avoidant mechanisms. However, these insects also demonstrated an increased susceptibility to freezing later in the season, which may be tied to changes in gut microbiota. Our results found shifts in the composition of the gut microbiome in R. flavipes between mid- to late summer and early to late fall. These differences may be suggestive of a change in metabolism to adjust to a period of reduced feeding and increased metabolic stress during overwintering. Specifically, results showed an increased abundance of Methanobrevibacter sp. (Euryarchaeota) associated with cold, which may be indicative of a metabolic shift from acetogenesis to methanogenesis associated with overwintering. Further work is needed focusing on specific contributions of certain gut microbes, particularly their role in metabolic adaptability and in providing protection from oxidative stress associated with changes in environmental conditions.

This research evaluated the mechanical and physical properties of three underutilized hardwood species according to visual lumber grades. Findings are discussed in relation to the requirements recommended such as density, modulus of rupture (MOR), and modulus of elasticity (MOE) for the manufacture of cross-laminated timber (CLT) industrial mats, which is a potential end use of low-valued hardwood species. Results showed that all species tested had an average MOE greater than that required for CLT lumber. Visual grading was an important component in strength determinations as evidenced by the correlation between MOE and MOR. All grades of red oak (Quercus rubra L.) had higher densities than those of southern yellow pine, which is the traditional wood used in manufacturing CLT industrial mats, with a density of 616 kg/m³ at 12 percent moisture; the average density of the red oak tested was 770 kg/m³ at 13.98 percent moisture. All grades of red oak also had MOE and MOR values that met the requirements for lumber used in the manufacture of CLT industrial mats. For sweetgum (Liquidambar styraciflua L.), all grades met the required values for MOE and MOR, but only Grades 3 and 4 met the required density. All grades of yellow poplar (Liriodendron tulipifera L.) met the required MOE and MOR, but none met the required density.

The wood of African mahogany (Khaya spp.) is known for its high quality and similarity to the more expensive Brazilian mahogany (Swietenia macrophylla King). Interest in African mahogany has been increasing because of the reduced availability of native Brazilian mahogany, which has become illegal to harvest. Most African mahogany comes from managed plantations, so the increased consumption of this wood helps to alleviate issues related to illegal harvesting by reducing the trade of Brazilian mahogany. African mahogany has also been gaining popularity in the United States, particularly for use in the wood flooring market. However, there is lack of technical information on the properties of African mahogany material available in the US market, therefore, the objective of this work was to describe anatomical features, evaluate physical–mechanical properties and examine natural resistance of African mahogany wood that is commercially available in the US market. The results of this research provides an increase in knowledge on African mahogany wood properties, natural resistance, and provides technical information that is needed by industry.