Cannabis sativa (hemp) is a source of various biologically active compounds, for instance, cannabinoids, terpenes and phenolic compounds, which exhibit antibacterial, antifungal, anti-inflammatory and anticancer properties. With the purpose of expanding the auxiliary application of C. sativa in the field of bio-nanotechnology, we explored the plant for green and efficient synthesis of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs). The nanoparticles were synthesized by utilizing an aqueous extract of C. sativa stem separated into two different fractions (cortex and core [xylem part]) without any additional reducing, stabilizing and capping agents. In the synthesis of AuNPs using the cortex enriched in bast fibers, fiber-AuNPs (F-AuNPs) were achieved. When using the core part of the stem, which is enriched with phenolic compounds such as alkaloids and cannabinoids, core-AuNPs (C-AuNPs) and core-AgNPs (C-AgNPs) were formed. Synthesized nanoparticles were character-ized by UV–visible analysis, transmission electron microscopy, atomic force microscopy, dynamic light scattering, Fourier transform infrared, and matrix-assisted laser desorption/ionization time-of-flight. In addition, the stable nature of nanoparticles has been shown by thermogravimetric analysis and inductively coupled plasma mass spectrometry (ICP-MS). Finally, the AgNPs were explored for the inhibition of Pseudomonas aeruginosa and Escherichia coli biofilms. The synthesized nanoparticles were crystalline with an average diameter between 12 and 18 nm for F-AuNPs and C-AuNPs and in the range of 20–40 nm for C-AgNPs. ICP-MS analysis revealed concentrations of synthesized nanoparticles as 0.7, 4.5 and 3.6 mg/mL for F-AuNPs, C-AuNPs and C-AgNPs, respectively. Fourier transform infrared spectroscopy revealed the presence of flavonoids, cannabinoids, terpenes and phenols on the nanoparticle surface, which could be responsible for reducing the salts to nanoparticles and further stabilizing them. In addition, the stable nature of synthesized nanoparticles has been shown by thermogravimetric analysis and ICP-MS. Finally, the AgNPs were explored for the inhibition of P. aeruginosa and E. coli biofilms. The nanoparticles exhibited minimum inhibitory concentration values of 6.25 and 5 µg/mL and minimum bactericidal concentration values of 12.5 and 25 µg/mL against P. aeruginosa and E. coli, respectively.

The rapid depletion and environmental concerns associated with the use of fossil fuels has led to extensive development of biofuels such as bioethanol from seaweeds. The long-term prospect of seaweed bioethanol production however, depends on the selection of processes in the hydrolysis and fermentation stages due to their limiting effect on ethanol yield. This review explored the factors influencing the hydrolysis and fermentation stages of seaweed bioethanol production with emphasis on process efficiency and sustainable application. Seaweed carbohydrate contents which are most critical for ethanol production substrate selection were 52 ± 6%, 55 ± 12% and 57 ± 13% for green, brown and red seaweeds, respectively. Inhibitor formation and polysaccharide selectivity were found to be the major bottlenecks influencing the efficiency of dilute acid and enzymatic hydrolysis, respectively. Current enzyme preparations used, were developed for starch-based and lignocellulosic biomass but not seaweeds, which differs in polysaccharide composition and structure. Also, the identification of fermenting organisms capable of converting the heterogeneous monomeric sugars in seaweeds is the major factor limiting ethanol yield during the fermentation stage and not the SHF or SSF pathway selection. This has resulted in variations in bioethanol yields, ranging from 0.04 g/g DM to 0.43 g/g DM.

Classical field retting and controlled fungal retting of hemp using Phlebia radiata Cel 26 (a mutant with low cellulose degrading ability) were compared with pure pectinase treatment with regard to mechanical properties of the produced fibre/epoxy composites. For field retting a classification of the microbial evolution (by gene sequencing) and enzyme profiles were conducted. By phylogenetic frequency mapping, different types of fungi, many belonging to the Ascomycota phylum were found on the fibres during the first 2 weeks of field retting, and thereafter, different types of bacteria, notably Proteobacteria, also proliferated on the field retted fibres. Extracts from field retted fibres exhibited high glucanase activities, while extracts from P. radiata Cel 26 retted fibres showed high polygalacturonase and laccase activities. As a result, fungal retting gave a significantly higher glucan content in the fibres than field retting (77 vs. 67%) and caused a higher removal of pectin as indicated by lower galacturonan content of fibres (1.6%) after fibres were retted for 20 days with P. radiata Cel 26 compared to a galacturonan content of 3.6% for field retted fibres. Effective fibre stiffness increased slightly after retting with P. radiata Cel 26 from 65 to 67 GPa, while it decreased after field retting to 52 GPa. Effective fibre strength could not be determined similarly due to variations in fibre fracture strain and fibre-matrix adhesion. A maximum composite strength with 50 vol% fibres of 307 MPa was obtained using P. radiata Cel 26 compared to 248 MPa with field retting.

Municipal organic waste (biowaste) consists of food derived starch, protein and sugars, and lignocellulose derived cellulose, hemicellulose, lignin and pectin. Proper management enables nutrient recycling and sustainable production of platform chemicals such as lactic acid (LA). This review gathers the most important information regarding use of biowaste for LA fermentation covering pre-treatment, enzymatic hydrolysis, fermentation and downstream processing to achieve high purity LA. The optimal approach was found to treat the two biowaste fractions separately due to different pre-treatment and enzyme needs for achieving enzymatic hydrolysis and to do continues fermentation to achieve high cell density and high LA productivity up to 12 g/L/h for production of both L and D isomers. The specific productivity was 0.4 to 0.5 h^-1 but with recalcitrant biomass, the enzymatic hydrolysis was rate limiting. Novel purification approaches included reactive distillation and emulsion liquid membrane separation yielding purities sufficient for polylactic acid production.