This page has only limited features, please log in for full access.
Date palm fruit (Phoenix dactylifera) is reputed to have numerous biological activities, including anticancer properties. To utilize the great fortune of this fruit, the current study aimed to maximize its pharmacological activity. Date palm extract (DPE) of Khalas cultivar was obtained in powder form and then was formulated into nanoemulsion (NE). The optimized DPE-NE was formulated along with its naked counterpart followed by studying their physical and chemical properties. A qualitative assessment of total serum protein associated with the surface of formulations was implemented. Studies for the in vitro release of DPE from developed NE before and after incubation with serum were investigated. Eventually, an MTT assay was conducted. Total phenolic and flavonoid contents were 22.89 ± 0.013 mg GAE/g of dry DPE and 9.90 ± 0.03 mg QE/g of dry DPE, respectively. Homogenous NE formulations were attained with appropriate particle size and viscosity that could be administered intravenously. The optimized PEGylated NE exhibited a proper particle size, PDI, and zeta potential. Total serum protein adsorbed on PEG-NE surface was significantly low. The release of the drug through in vitro study was effectively extended for 24 h. Ultimately; PEGylated NE of DPE attained significant inhibition for cancer cell viability with IC50 values of 18.6 ± 2.4 and 13.5 ± 1.8 µg/mL for MCF-7 and HepG2 cell lines, respectively. PEGylated NE of DPE of Khalas cultivar will open the gate for future adjuvants for cancer therapy.
Hany Khalil; Nashi Alqahtani; Hossam Darrag; Hairul-Islam Ibrahim; Promise Emeka; Lorina Badger-Emeka; Katsuyoshi Matsunami; Tamer Shehata; Heba Elsewedy. Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation. Plants 2021, 10, 735 .
AMA StyleHany Khalil, Nashi Alqahtani, Hossam Darrag, Hairul-Islam Ibrahim, Promise Emeka, Lorina Badger-Emeka, Katsuyoshi Matsunami, Tamer Shehata, Heba Elsewedy. Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation. Plants. 2021; 10 (4):735.
Chicago/Turabian StyleHany Khalil; Nashi Alqahtani; Hossam Darrag; Hairul-Islam Ibrahim; Promise Emeka; Lorina Badger-Emeka; Katsuyoshi Matsunami; Tamer Shehata; Heba Elsewedy. 2021. "Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation." Plants 10, no. 4: 735.
The short non-coding microRNAs (miRNAs), have emerged as reliable modulators of various pathological conditions including autoimmune diseases in mammals. The current study, aims to identify new potential differential expressed miRNAs and their downstream mRNA targets of the autoimmune disease, Multiple sclerosis (MS). First, we used a computational tool to identify a new set of miRNA(s) that are probably implicated in MS. Preliminary, computational screening reveals that miR-659-3p, miR-659-5p, miR- 684, miR-3607-3p, miR-3607-5p, miR-3682-3p, miR-3682-5p miR-4647, miR-7188-3p, miR-7188-5p and miR-7235 are specifically elevated in the secondary lymphoid cells of EAE mice. In addition, expression of the downstream target genes of these miRNAs such as FXBO33, SGMS-1, ZDHHC-9, GABRA-3, NRXN-2 were reciprocal to miRNA expression in lymphoid cells. These confirmed by applying the mimic and silencing miRNA models, these data suggesting new inflammatory target genes of these promising miRNA biomarkers. The in vivo adoptive transfer model revealed that the suppression of miRNA-7188-5p and miR-7235 changed the pattern of astrocytes and CNSpathophysiology. The current study identified set of miRNAs and their mRNA targets as reciprocal regulator in MS disease. The absence of miRNA-7188-5p and miR-7235 enhanced the disease alleviation. These optimized results highlight new set of miRNA’s based biomarkers with therapeutic potential in experimental MS.
Hairul Islam Mohamed Ibrahim; Abdullah; Hamza Hanieh; Emad A Ahmed; K Thirugnansambantham. Differential expression of microRNA-7188-5p and miR-7235 regulates Multiple Sclerosis in an experimental mouse model- an Biomarker approach. 2021, 1 .
AMA StyleHairul Islam Mohamed Ibrahim, Abdullah, Hamza Hanieh, Emad A Ahmed, K Thirugnansambantham. Differential expression of microRNA-7188-5p and miR-7235 regulates Multiple Sclerosis in an experimental mouse model- an Biomarker approach. . 2021; ():1.
Chicago/Turabian StyleHairul Islam Mohamed Ibrahim; Abdullah; Hamza Hanieh; Emad A Ahmed; K Thirugnansambantham. 2021. "Differential expression of microRNA-7188-5p and miR-7235 regulates Multiple Sclerosis in an experimental mouse model- an Biomarker approach." , no. : 1.
Allergy is an immunological disorder that develops in response to exposure to an allergen, and histamines mediate these effects via histidine decarboxylase (HDC) activity at the intracellular level. In the present study, we developed a 3D model of Klebsiella pneumoniae histidine decarboxylase (HDC) and analyzed the HDC inhibitory potential of cinnamaldehyde (CA) and subsequent anti-allergic potential using a bacterial and mammalian mast cell model. A computational and in vitro study using K. pneumonia revealed that CA binds to HDC nearby the pyridoxal-5′-phosphate (PLP) binding site and inhibited histamine synthesis in a bacterial model. Further study using a mammalian mast cell model also showed that CA decreased the levels of histamine in the stimulated RBL-2H3 cell line and attenuated the release of β-hexoseaminidase and cell degranulation. In addition, CA treatment also significantly suppressed the levels of pro-inflammatory cytokines TNF-α and IL-6 and the nitric oxide (NO) level in the stimulated mast cells. A gene expression and Western blotting study revealed that CA significantly downregulated the expressions of MAPKp38/ERK and its downstream pro-allergic mediators that are involved in the signaling pathway in mast cell cytokine synthesis. This study further confirms that CA has the potential to attenuate mast cell activation by inhibiting HDC and modifying the process of allergic disorders.
Lorina Badger-Emeka; Promise Emeka; Krishnaraj Thirugnanasambantham; Hairul Ibrahim. Anti-Allergic Potential of Cinnamaldehyde via the Inhibitory Effect of Histidine Decarboxylase (HDC) Producing Klebsiella pneumonia. Molecules 2020, 25, 5580 .
AMA StyleLorina Badger-Emeka, Promise Emeka, Krishnaraj Thirugnanasambantham, Hairul Ibrahim. Anti-Allergic Potential of Cinnamaldehyde via the Inhibitory Effect of Histidine Decarboxylase (HDC) Producing Klebsiella pneumonia. Molecules. 2020; 25 (23):5580.
Chicago/Turabian StyleLorina Badger-Emeka; Promise Emeka; Krishnaraj Thirugnanasambantham; Hairul Ibrahim. 2020. "Anti-Allergic Potential of Cinnamaldehyde via the Inhibitory Effect of Histidine Decarboxylase (HDC) Producing Klebsiella pneumonia." Molecules 25, no. 23: 5580.
Glucansucrase secreted by Streptococcus mutans and composed of virulence genes alters oral microbiota, creating adherent environment for structural bacteria colony forming dental biofilm. The present investigation studied the inhibitory and binding potentials of mangiferin against S. mutans and its enzyme glucansucrase implicated in biofilm formation. Antibacterial activity against planktonic S. mutans was carried out. Using reverse transcription PCR, the expression of crucial virulence genes, gtfB, gtfC, gtfD, gbpB, and comDE were determined. The effect of mangiferin on teeth surfaces biofilm was ascertained by scanning electron microscopy (SEM). Docking analysis of S. mutans glucansucrase and mangiferin revealed the binding energy of −7.35 and ten hydrogen interactions. Antibacterial study revealed that mangiferin was not lethal to planktonic S. mutans, but a concentration-dependent inhibition of glucansucrase activity was observed. The inhibitory effect of water-insoluble glucan synthesis was apparently more marked relative to water-soluble glucan synthesis attenuation. Mangiferin significantly downregulated the expression of the virulence genes, indicating a mechanism involving glucanotranferases, specifically inhibiting colony formation by attenuating bacterial adherence. SEM images revealed that S. mutans biofilm density was scanty in mangiferin treated teeth compared to non-treated control teeth. Our data therefore suggest that mangiferin inhibited S. mutans biofilms formation by attenuating glucansucrase activities without affecting bacteria growth.
Promise Emeka; Lorina Badger-Emeka; Hairul-Islam Ibrahim; Krishnaraj Thirugnanasambantham; Jamal Hussen. Inhibitory Potential of Mangiferin on Glucansucrase Producing Streptococcus mutans Biofilm in Dental Plaque. Applied Sciences 2020, 10, 8297 .
AMA StylePromise Emeka, Lorina Badger-Emeka, Hairul-Islam Ibrahim, Krishnaraj Thirugnanasambantham, Jamal Hussen. Inhibitory Potential of Mangiferin on Glucansucrase Producing Streptococcus mutans Biofilm in Dental Plaque. Applied Sciences. 2020; 10 (22):8297.
Chicago/Turabian StylePromise Emeka; Lorina Badger-Emeka; Hairul-Islam Ibrahim; Krishnaraj Thirugnanasambantham; Jamal Hussen. 2020. "Inhibitory Potential of Mangiferin on Glucansucrase Producing Streptococcus mutans Biofilm in Dental Plaque." Applied Sciences 10, no. 22: 8297.
Trigonella stellata has used in folk medicine as palatable and nutraceutical herb. It also regulates hypocholesterolemia, hypoglycemia, and has showed anti-inflammatory activities as well as antioxidants efficacy. Osteoporosis is a one of bone metabolic disorders and is continuously increasing worldwide. In the present study, caffeic acid was isolated from Trigonella stellata and identified using 1 D- and 2 D-NMR spectroscopic data. Caffeic acid was investigated on osteoblast and osteoclast in vitro using mice bone marrow-derived mesenchymal cells. Caffeic acid played reciprocal proliferation between osteoblast and osteoclast cells and accelerated the bone mineralization. It was confirmed by cytotoxicity, alkaline phosphatase (ALP), alizarin red S (ARS), and Tartrate resistant acid phosphatase (TRAP) assay. Caffeic acid regulated the osteogenic marker and upregulated the osteopontin, osteocalcin, and bone morphogenic proteins (BMP). Quantitative real time PCR and Western blot were used to quantify the mRNA and protein markers. It also regulated the matrix metalloprotease-2 (MMP-2) and cathepsin-K proteolytic markers in osteoclast cells. In addition, caffeic acid inhibited bone resorption in osteoclast cells. On the other hand, it upregulate osteoblast differentiation through stimulation of extracellular calcium concentrations osteoblast differentiation, respectively. The results also were confirmed through in silico docking of caffeic acid against cathepsin-B and cathepsin-K markers. These findings revealed that caffeic acid has a potential role in bone-metabolic disorder through its multifaceted effects on osteoblast and osteoclast regulations and controls osteoporosis.
Hairul-Islam Mohamed Ibrahim; Hossam M. Darrag; Mohammed Refdan Alhajhoj; Hany Ezzat Khalil. Biomolecule from Trigonella stellata from Saudi Flora to Suppress Osteoporosis via Osteostromal Regulations. Plants 2020, 9, 1610 .
AMA StyleHairul-Islam Mohamed Ibrahim, Hossam M. Darrag, Mohammed Refdan Alhajhoj, Hany Ezzat Khalil. Biomolecule from Trigonella stellata from Saudi Flora to Suppress Osteoporosis via Osteostromal Regulations. Plants. 2020; 9 (11):1610.
Chicago/Turabian StyleHairul-Islam Mohamed Ibrahim; Hossam M. Darrag; Mohammed Refdan Alhajhoj; Hany Ezzat Khalil. 2020. "Biomolecule from Trigonella stellata from Saudi Flora to Suppress Osteoporosis via Osteostromal Regulations." Plants 9, no. 11: 1610.
Background/Aims: The role of mobile genetic elements such as plasmids in the spread of multiple antibiotic resistance has continued to receive attention by researchers. Due to the diverse nature of Escherichia coli strains characteristically harbouring large proportion of genomic plasmids, the aim of the present study is to evaluate the antimicrobial susceptibility and plasmid profiles of clinical isolates of E. coli in this south eastern region of Saudi Arabia. Methods: Bacterial isolation, antimicrobial susceptibility test were carried out using basic microbiological techniques. Dendrogram of clustering in relationship to antibiotic susceptibility of the isolates was carried out using PAST. Extracted plasmid DNA was visualised using the ultraviolet light illuminator and photo documentation system. Sizes of Plasmid were determined using standard DNA molecular weight markers at 1kb ladder. Plasmid curing was carried out using Sodium Dodecyl Sulphate (SDS). Results and Conclusion: Antibiotic resistance ranged between 85.71%-100% for the cephalosporins, 64.29% to 92.9% resistance for penicillins, including Aztreonam, a last line drug. Results indicate that 70% of the isolates did not show any plasmid presence. However, 30% of the isolates contained plasmids with molecular weights of 30kb and above. Dendrogram of clustering in relationship to antibiotic susceptibility showed isolates were in 3 clades pre-plasmid curing and 6 clades post-plasmid curing. One isolate (EC36) was cured of its resistance to Penicillins, while isolates EC67, EC77 and EC87 initially sensitive to ciprofloxacin, became resistant to the antibiotic. The findings suggest a preponderance of chromosomal mediated resistant E. coli. The few plasmid containing resistant bacteria, were resistant to curing as they displayed continued antibiotic resistance after curing. This therefore confirms the growing presence of multiple genomic plasmid harbouring E. coli.
Badger-Emeka Lorina Ineta; Lorina Ineta; Emeka Promise Madu; Al-Sultan Abdulrahman Abdulhadi; Hairul-Islam Villianur Ibrahim. Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli. Biomedical Research 2018, 29, 1 .
AMA StyleBadger-Emeka Lorina Ineta, Lorina Ineta, Emeka Promise Madu, Al-Sultan Abdulrahman Abdulhadi, Hairul-Islam Villianur Ibrahim. Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli. Biomedical Research. 2018; 29 (17):1.
Chicago/Turabian StyleBadger-Emeka Lorina Ineta; Lorina Ineta; Emeka Promise Madu; Al-Sultan Abdulrahman Abdulhadi; Hairul-Islam Villianur Ibrahim. 2018. "Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli." Biomedical Research 29, no. 17: 1.
Chronic inflammation in ulcerative colitis (UC) patients is the major risk factor for colitis-associated colon cancer (CAC). Recent evidences have shown that microRNAs (miRNAs) are implicated in CAC pathogenesis. However, the interaction of miRNAs with the transcription factors that alleviate CAC has not been reported. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or 3,3'-diindolylmethane (DIM) were used to activate aryl hydrocarbon receptor (Ahr) in azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced CAC in mice. Real-time PCR was used to quantify the mRNAs of miRNA and coding genes while western blot and ELISA were used to quantify protein levels. Silencing miRNA was carried out by means of electroporation and locked nucleic acid (LNA)-miRNA. Inducing CAC in mice upregulated miR-132 expression in the colon, spleen and lymph nodes at all stages of disease development. Activation of Ahr by TCDD or DIM boosted miR-132 expression and alleviated CAC severity by suppression of macrophage infiltration and pro-inflammatory cytokines. Interestingly, TCDD, but not DIM, augmented a cholinergic anti-inflammation by inducing acetylcholinesterase (AChE)-targeting miR-132. This anti-inflammation was manifested by suppressed production of TNF-α, IL-1β and IL-6. Silencing miR-132 in vivo in TCDD-treated mice abrogated the cholinergic anti-inflammation and exacerbated CAC. In addition, inhibition of miR-132 in vitro in CD4+ cells and macrophages mitigated the inhibitory effect of TCDD on AChE catalytic activity. Our findings identify miR-132 as a new molecule implicated in CAC pathogenesis, and reveal that miR-132 mediates the ameliorating effects of TCDD on CAC, suggesting miR-132 as a promising therapeutic candidate to control autoimmune inflammation and tumorigenesis in CAC patients.
Abdullah M. Alzahrani; Hamza Hanieh; Hairul-Islam Villianur Ibrahim; Omar Mohafez; Tamer Shehata; Mohammad Bani Ismail; Manal Alfwuaires. Enhancing miR-132 expression by aryl hydrocarbon receptor attenuates tumorigenesis associated with chronic colitis. International Immunopharmacology 2017, 52, 342 -351.
AMA StyleAbdullah M. Alzahrani, Hamza Hanieh, Hairul-Islam Villianur Ibrahim, Omar Mohafez, Tamer Shehata, Mohammad Bani Ismail, Manal Alfwuaires. Enhancing miR-132 expression by aryl hydrocarbon receptor attenuates tumorigenesis associated with chronic colitis. International Immunopharmacology. 2017; 52 ():342-351.
Chicago/Turabian StyleAbdullah M. Alzahrani; Hamza Hanieh; Hairul-Islam Villianur Ibrahim; Omar Mohafez; Tamer Shehata; Mohammad Bani Ismail; Manal Alfwuaires. 2017. "Enhancing miR-132 expression by aryl hydrocarbon receptor attenuates tumorigenesis associated with chronic colitis." International Immunopharmacology 52, no. : 342-351.