Background: Gut is a crucial organ for the host’s defense system due to its filtering action of the intestinal membrane from hazardous foreign substances. One strategy to strengthen the gut epithelial barrier function is to upregulate beneficial microflora populations and their metabolites. Sophorolipid (SPL), which is a glycolipid bio-surfactant, could increase beneficial microflora and decrease pathogenic bacteria in the gastrointestinal tract. Therefore, herein, we conducted an experiment with broiler chickens to investigate the fortifying effects of SPL on the host’s gut defense system by modulating the microbiota population. Methods: A total of 540 1-day-old chicks (Ross 308) were used, and they were immediately allotted into three treatment groups (6 replications with 30 chicks/pen) according to their initial body weight. The dietary treatments consisted of CON (basal diet), BAM (10 mg/kg bambermycin), and SPL (10 mg/kg SPL). During the experiment, birds freely accessed feed and water, and body weight and feed intake were measured at the end of each phase. On d 35, birds (one bird/pen) were sacrificed to collect jejunum and cecum samples. Results: Dietary SPL and BAM supplementation significantly accelerated birds’ growth and also significantly improved feed efficiency compared to CON. Intestinal microbial community was significantly separated by dietary SPL supplementation from that of CON, and dietary SPL supplementation significantly increased Lactobacillus spp. and Akkermansia muciniphila. Moreover, birds fed with dietary SPL also showed the highest concentration of cecal butyrate among all treatment groups. Gut morphological analysis showed that dietary SPL significantly increased villus height, ratio of villus height to crypt depth, goblet cell numbers, and the gene expression levels of claudin-1 and mucin 2. Additionally, dietary SPL significantly decreased the mRNA expression level of pro-inflammatory cytokine, interleukin-6, and increased that of anti-inflammatory cytokine, interleukin-10, compared to other treatments. Conclusions: Dietary SPL increases the beneficial bacterial population and butyrate concentration, which leads to a strengthened gut barrier function. In addition, the intestinal inflammation was also downregulated by dietary SPL supplementation.

In this study, a haptic device is proposed for virtual reality (VR) applications. The proposed haptic device is linked to the bundle controller of a VR device. The device proposed in this study is a force-feedback device that focuses on the sensation felt by the hand rather than the feeling of the tool colliding with the object. The device provides sensations of centrifugal or inertial forces due to the weight of the tool when the user swings the tool and the sensation of a reaction force when the user stabs or pokes an object with the tool. The force generated by a haptic device of the linked type was measured and examined to determine whether a sensation of actually using a tool in the virtual environment was provided. In addition, we conducted two types of experiments to evaluate the proposed haptic device. First, VR content was created for the user experience, and a survey was conducted with 30 experimenters to compare the bundle controllers and a haptic device, called HapTug, in terms of realism, immersion, and enjoyment. The survey results demonstrated that the realism, immersion, and enjoyment increased compared to using the bundle controller alone. A total of 41 experimenters were allowed to play freely in a virtual environment for six sessions of 30 s each. They pressed the reset session button when they felt 30 s had passed, and the device they were wearing turned on and off randomly every session. During the experience, their playback time, number of interactions, and range were measured. As a result, when the device was activated, experimenters felt the time was shorter and showed widespread movement. Thus, it was proven that the proposed linked-type haptic device effectively delivered the feeling of wielding a tool, allowing the user to feel the virtual object coming in and going out of the hand when interacting with it. Moreover, three conclusions were drawn from the results of the experiments: previous VR experience times have little to do with satisfaction related to haptic device use, users have low expectations for haptic realism, and users interact more actively and exaggeratedly with a haptic device.

Dogs often express their stress through physical motions that can be recognized by their owners. We propose a mobile application that analyzes companion dog’s behavior and their three-dimensional poses via deep learning. As existing research on pose estimation has focused on humans, obtaining a large dataset comprising images showing animal joint locations is a challenge. Nevertheless, we generated such a dataset and used it to train an AI model. Furthermore, we analyzed circling behavior, which is associated with stress in companion dogs. To this end, we used the VideoPose3D model to estimate the 3D poses of companion dogs from the 2D pose estimation technique derived by the DeepLabCut model and developed a mobile app that provides analytical information on the stress-related behaviors, as well as the walking and isolation times, of companion dogs. Finally, we interviewed five certified experts to evaluate the validity and applicability of the app.

Background: In animals, weaning stress is the first and most critical stress. Weaning can negatively affect the growth performance of animals physically, psychologically, and pathologically. Our previous studies on the HT-29 cell line and early-weaned rats demonstrated that adequate sophorolipid (SPL) supplementation in feed could enhance the mucin-producing and wound healing capacities of the gut defense system by modulating gut microbiota. Methods: We conducted an experiment with one hundred forty 21-day-old early weaned piglets (L x Y x D). They were allocated into 4 treatment and 7 replications (4 pigs per pen) according to their initial body weight. Body weight and feed intake were measured biweekly during experimental period. After 6 weeks, 28 pigs were randomly selected and sacrificed to collect plasma, jejunum, and cecal content samples. Results: Dietary SPL supplementation at 5 and 10 mg/kg quadratically increased the average daily gain during the experimental period in the treatment groups when compared with the control group. The albumin levels of piglets fed with the SPL supplemented diet were downregulated to the normal range. Moreover, in feed, SPL supplementation at 5 and 10 mg/kg improved jejunal histological indices and gene expression levels related to mucin secretion and local inflammation markers. Consistent with these results, adequate SPL supplementation (5 and 10 mg/kg) increased the population of Prevotella, a beneficial bacterium, and its short-chain fatty acid production in the ceca of piglets. Conclusions: The occurrence of diarrhea after weaning in piglets could be reduced by feeding a 10 ppm of SPL supplemented diet which improves the gut defense system by improving the microbial population and enhancing mucin layer integrity.

Dietary supplementation of sophorolipid could protect from damage of dextran sodium sulphate by improvement of mucus layer and gut bacterial metabolites.

Dietary fat and oil could aid in reaching the high-energy requirements of fast-growing birds; however, these inclusions could lead to nutrient waste. This is because young birds have limited lipid digestion due to the low secretion of lipase and bile salt. Sophorolipid (SPL), a glycolipid emulsifier with lower toxicity and higher biodegradability, can upregulate fat utilization by increasing digestibility. Accordingly, a five-week-long experiment was conducted with 720 one-day-old chicks (Ross 308) to investigate the effects of dietary SPL on growth, organ characteristics, and gut health. The allotment was partitioned into four treatment groups according to their body weight with six replications (30 chick/pen). The three treatment diets comprised a basal diet with a formulation that met the Ross 308 standard and 5, 10, and 15 ppm SPL in the basal diet. During the experiment, the birds had free access to feed, and body weight and feed intake were measured at the end of each phase. Chickens were put down at the end of the growing and finishing phases, and jejunum and cecal samples were obtained to investigate organ characteristics and gut environments. The data were analyzed using the generalized linear model procedures of SAS 9.4, and all data were assessed for linear, quadratic, and cubic effects of dietary SPL-supplemented dosages. Body weight was significantly increased with 10 ppm of SPL supplementation in the grower phase without affecting feed efficiency. The relative weights of the intestine and the bursa of Fabricius were quadratically decreased by SPL supplementation with a lower population of Streptococcus and higher propionate and butyrate concentrations. Additionally, the dietary SPL supplementation groups showed a significantly increased villus/crypt ratio with higher intestinal expression levels of fatty acid translocase, diacylglycerol acyltransferase 2, and fatty acid transporter 4. Collectively, proper SPL supplementation in the chicken diet could improve growth performance by down-regulating immune modulation and up-regulating lipid digestion and absorption via modulation of gut microenvironments.