Almond canker diseases are destructive and can reduce the yield as well as the lifespan of almond orchards. These diseases may affect the trunk and branches of both young and mature trees and can result in tree death soon after orchard establishment in severe cases. Between 2015 and 2018, 70 almond orchards were visited throughout the Central Valley of California upon requests from farm advisors for canker disease diagnosis. Two major canker diseases were identified, including Botryosphaeriaceae cankers and Ceratocystis canker. In addition, five less prevalent canker diseases were identified, including Cytospora, Eutypa, Diaporthe, Collophorina, and Pallidophorina canker. Seventy-four fungal isolates were selected for multilocus phylogenetic analyses of internal transcribed spacer region ITS1-5.8S-ITS2 and part of the translation elongation factor 1-α, β-tubulin, and glyceraldehyde 3-phosphate dehydrogenase gene sequences; 27 species were identified, including 12 Botryosphaeriaceae species, Ceratocystis destructans, five Cytospora species, Collophorina hispanica, four Diaporthe species, two Diatrype species, Eutypa lata, and Pallidophorina paarla. The most frequently isolated species were Ceratocystis destructans, Neoscytalidium dimidiatum, and Cytospora californica. Pathogenicity experiments on almond cultivar Nonpareil revealed that Neofusicoccum parvum, Neofusicoccum arbuti, and Neofusicoccum mediterraneum were the most virulent. Botryosphaeriaceae cankers were predominantly found in young orchards and symptoms were most prevalent on the trunks of trees. Ceratocystis canker was most commonly found in mature orchards and associated with symptoms found on trunks or large scaffold branches. This study provides a thorough examination of the diversity and pathogenicity of fungal pathogens associated with branch and trunk cankers of almond in California.

Prevalence of latent infections of the canker-causing fungi Botryosphaeria dothidea and species of Cytospora, Diplodia, Lasiodiplodia, Neofusicoccum, and Phomopsis in young shoots of almond, prune, and walnut trees in California was studied to test the hypotheses that latent infections accumulate from current-season shoots to 1-year-old shoots in the orchard and there are distinct associations among pathogen taxa present as latent infections in the same shoot. Samples of newly emerged and 1-year-old shoots were periodically collected in each almond, prune, and walnut orchard for two growing seasons. A real-time quantitative PCR assay was used to quantify latent infection with three parameters: incidence, molecular severity, and latent infection index. Diplodia spp. were absent from most samples. For almond, Lasiodiplodia spp. and Cytospora spp. were detected with a maximum incidence >90%, while B. dothidea and Neofusicoccum spp. incidence was 50% in most cases, while those of Cytospora spp. and Lasiodiplodia spp. were 30 to 60% and 30 to 100%, respectively. For walnut, many samplings showed higher incidence in 1-year-old (30 to 80%) than in newly emerged shoots (10 to 50%). Accumulation of latent infection between the two shoot age classes was detected in only a few cases. The percentages of samples showing coexistence of two, three, and four pathogen taxa in the same shoot were 20 to 25, <10, and <5%, respectively. Pairwise associations among pathogen taxa in the same shoot were significant in many cases.

Almond band canker and prune Cytospora canker have become more severe in the last decade, especially in young orchards, in California. To test our hypothesis that young trees from nurseries could carry the canker-causing pathogens at latency phase to new orchards through transplanting, a multiyear survey on latent infection of canker-causing pathogens of budwood and young trees of almond and prune nurseries in California was conducted. A total of more than 1,730 samples including shoots of rootstocks and scions and grafting union cuttings were collected from 11 nurseries. A real-time quantitative PCR assay was applied to quantify the latent infection levels by six canker-causing pathogen taxa: Botryosphaeria dothidea and species of Cytospora, Diplodia, Lasiodiplodia, Neofusicoccum, and Phomopsis. For almond, the average incidences of latent infection caused by Lasiodiplodia spp. (43.6%) and Neofusicoccum spp. (24.2%) were significantly greater than those by the other four pathogen taxa. The molecular severity (MS) of latent infection caused by Neofusicoccum spp. (3.6) was significantly greater than those caused by other pathogen taxa, except for Lasiodiplodia spp. (2.6). For prune, the average incidence of latent infection caused by Cytospora spp. (13.5%) was significantly higher than those caused by B. dothidea (1.5%) and Diplodia spp. (1.3%) but not significantly higher than those caused by Lasiodiplodia spp. (6.9%), Neofusicoccum spp. (6.3%), and Phomopsis spp. (7.7%), respectively. Moreover, the average MS values caused by Cytospora spp. (3.8) and Neofusicoccum spp. (3.2) were the highest followed by those caused by B. dothidea (1.4), Lasiodiplodia spp. (2.2), and Phomopsis spp. (2.3). Different almond varieties showed various levels of susceptibilities to different canker-causing pathogens. This study concluded that Lasiodiplodia and Neofusicoccum are the predominant pathogen species in almond, and Cytospora is the most important canker-causing pathogen species in prune in nurseries. These findings confirmed the observations of predominancy of canker-causing pathogens in almond and prune orchards in California.

Unmanaged spray drift from orchard pesticide application contributes to environmental contamination and causes significant danger to farmworkers, nearby residential areas, and neighbors’ crops. Most drift control approaches do not guarantee adequate and uniform canopy spray coverage. Our goal was to develop a spray backstop system that could block drifting from the top without any negative impact on spray coverage and on-target deposition. The design included a foldable mast and a shade structure that covered the trees from the top. We used a continuous loop sampling to assess and quantify the effectiveness of spray backstop on drift potential reduction. We also collected leaf samples from different sections of trees to compare on-target deposition and coverage. The results showed that the spray backstop system could significantly (p-Value < 0.01) reduce drift potential from the top (78% on average). While we did not find any statistical difference in overall canopy deposition with and without the backstop system, we observed some improvement in treetops deposition. This experiment’s output suggests that growers may be able to adjust their air-assist sprayers for a more uniform spray coverage without concern about the off-target movement of spray droplets when they employ the spray backstop system.

Off-ground almond harvesting is a promising alternative to conventional on-ground methods, reducing dust, microbial contamination, and insect damage. However, the high moisture content (MC) in off-ground harvested almonds requires timely mechanical drying to maintain product quality and safety. This study aimed to characterize insect damage, physical and thermal properties of off-ground harvested almonds, and their components (hulls, shells, and kernels) at early (EH) and standard (SH) harvest stages, and to provide foundational data for drying process modeling and optimization. To minimize losses from prematurely detached almonds, EH was conducted 3–4 weeks before SH, which followed local practices. Off-ground harvesting reduced infestation by 52% compared to conventional methods, demonstrating significant quality and safety advantages. EH samples consisted entirely of in-hull almonds, while SH almonds included three distinct fractions: in-hull almonds, in-shell almonds, and loose hulls. EH almonds exhibited higher MC, thermal conductivity, and specific heat, indicating greater energy demand during drying. Among components, hulls had the highest MC and thermal properties, followed by shells and kernels. These findings support a two-phase drying strategy for EH almonds, starting with high temperature for efficient hull moisture removal, followed by moderate temperature to preserve kernel quality. In SH almonds, distinct thermal behaviors between in-hull and in-shell fractions, along with significant thickness differences (24.5 ± 2.5 mm vs. 17.2 ± 1.3 mm), highlight the need for separate drying. A size-based sorting strategy prior to drying is recommended to enhance efficiency and product uniformity. Overall, this study provides essential data to guide harvest-stage-specific postharvest practices in the almond industry.