We performed nitrogen (N) budgets in the photic layer of three contrasting stations representing different trophic conditions in the western tropical South Pacific (WTSP) Ocean during austral summer conditions (February–March 2015). Using a Lagrangian strategy, we sampled the same water mass for the entire duration of each long-duration (5 days) station, allowing us to consider only vertical exchanges for the budgets. We quantified all major vertical N fluxes both entering (N ₂ fixation, nitrate turbulent diffusion, atmospheric deposition) and leaving the photic layer (particulate N export). The three stations were characterized by a strong nitracline and contrasted deep chlorophyll maximum depths, which were lower in the oligotrophic Melanesian archipelago (MA, stations LD A and LD B) than in the ultra-oligotrophic waters of the South Pacific Gyre (SPG, station LD C). N ₂ fixation rates were extremely high at both LD A (593 ± 51 µmol N m ⁻² d ⁻¹ ) and LD B (706 ± 302 µmol N m ⁻² d ⁻¹ ), and the diazotroph community was dominated by Trichodesmium . N ₂ fixation rates were lower (59 ± 16 µmol N m ⁻² d ⁻¹ ) at LD C, and the diazotroph community was dominated by unicellular N ₂ -fixing cyanobacteria (UCYN). At all stations, N ₂ fixation was the major source of new N (> 90 %) before atmospheric deposition and upward nitrate fluxes induced by turbulence. N ₂ fixation contributed circa 13–18 % of primary production in the MA region and 3 % in the SPG water and sustained nearly all new primary production at all stations. The e ratio ( e ratio = particulate carbon export ∕ primary production) was maximum at LD A (9.7 %) and was higher than the e ratio in most studied oligotrophic regions (< 5 %), indicating a high efficiency of the WTSP to export carbon relative to primary production. The direct export of diazotrophs assessed by qPCR of the nifH gene in sediment traps represented up to 30.6 % of the PC export at LD A, while their contribution was 5 and < 0.1 % at LD B and LD C, respectively. At the three studied stations, the sum of all N input to the photic layer exceeded the N output through organic matter export. This disequilibrium leading to N accumulation in the upper layer appears as a characteristic of the WTSP during the summer season.

Fine-scale physical structures and ocean dynamics strongly influence and regulate biogeochemical and ecological processes. These processes are particularly challenging to describe and understand because of their ephemeral nature. The OSCAHR (Observing Submesoscale Coupling At High Resolution) campaign was conducted in fall 2015 in which a fine-scale structure (1–10 km∕1–10 days) in the northwestern Mediterranean Ligurian subbasin was pre-identified using both satellite and numerical modeling data. Along the ship track, various variables were measured at the surface (temperature, salinity, chlorophyll a and nutrient concentrations) with ADCP current velocity. We also deployed a new model of the CytoSense automated flow cytometer (AFCM) optimized for small and dim cells, for near real-time characterization of the surface phytoplankton community structure of surface waters with a spatial resolution of a few kilometers and an hourly temporal resolution. For the first time with this optimized version of the AFCM, we were able to fully resolve Prochlorococcus picocyanobacteria in addition to the easily distinguishable Synechococcus. The vertical physical dynamics and biogeochemical properties of the studied area were investigated by continuous high-resolution CTD profiles thanks to a moving vessel profiler (MVP) during the vessel underway associated with a high-resolution pumping system deployed during fixed stations allowing sampling of the water column at a fine resolution (below 1 m). The observed fine-scale feature presented a cyclonic structure with a relatively cold core surrounded by warmer waters. Surface waters were totally depleted in nitrate and phosphate. In addition to the doming of the isopycnals by the cyclonic circulation, an intense wind event induced Ekman pumping. The upwelled subsurface cold nutrient-rich water fertilized surface waters and was marked by an increase in Chl a concentration. Prochlorococcus and pico- and nano-eukaryotes were more abundant in cold core waters, while Synechococcus dominated in warm boundary waters. Nanoeukaryotes were the main contributors ( > 50 %) in terms of pigment content (red fluorescence) and biomass. Biological observations based on the mean cell's red fluorescence recorded by AFCM combined with physical properties of surface waters suggest a distinct origin for two warm boundary waters. Finally, the application of a matrix growth population model based on high-frequency AFCM measurements in warm boundary surface waters provides estimates of in situ growth rate and apparent net primary production for Prochlorococcus (μ = 0.21 d⁻¹, NPP = 0.11 mg C m⁻³ d⁻¹) and Synechococcus (μ = 0.72 d⁻¹, NPP = 2.68 mg C m⁻³ d⁻¹), which corroborate their opposite surface distribution pattern. The innovative adaptive strategy applied during OSCAHR with a combination of several multidisciplinary and complementary approaches involving high-resolution in situ observations and sampling, remote-sensing and model simulations provided a deeper understanding of the marine biogeochemical dynamics through the first trophic levels.

The overall goal of OUTPACE (Oligotrophy to UlTra-oligotrophy PACific Experiment) was to obtain a successful representation of the interactions between planktonic organisms and the cycle of biogenic elements in the western tropical South Pacific Ocean across trophic and N₂ fixation gradients. Within the context of climate change, it is necessary to better quantify the ability of the oligotrophic ocean to sequester carbon through biological processes. OUTPACE was organized around three main objectives, which were (1) to perform a zonal characterization of the biogeochemistry and biological diversity of the western tropical South Pacific during austral summer conditions, (2) to study the production and fate of organic matter (including carbon export) in three contrasting trophic regimes (increasing oligotrophy) with a particular emphasis on the role of dinitrogen fixation, and (3) to obtain a representation of the main biogeochemical fluxes and dynamics of the planktonic trophic network. The international OUTPACE cruise took place between 18 February and 3 April 2015 aboard the RV L'Atalante and involved 60 scientists (30 onboard). The west–east transect covered ∼ 4000 km from the western part of the Melanesian archipelago (New Caledonia) to the western boundary of the South Pacific gyre (French Polynesia). Following an adaptive strategy, the transect initially designed along the 19° S parallel was adapted along-route to incorporate information coming from satellite measurements of sea surface temperature, chlorophyll a concentration, currents, and diazotroph quantification. After providing a general context and describing previous work done in this area, this introductory paper elucidates the objectives of OUTPACE, the implementation plan of the cruise and water mass and climatological characteristics and concludes with a general overview of the other papers that will be published in this special issue.

Model simulations and remote sensing observations show that ocean dynamics at fine scales (1–100 km in space, day–weeks in time) strongly influence the distribution of phytoplankton. However, only a few in situ-based studies at fine scales have been performed, and most of them concern western boundary currents which may not be representative of less energetic regions. The PROTEVSMED-SWOT cruise took place in the moderately energetic waters of the western Mediterranean Sea (WMS), in the region south of the Balearic Islands. Taking advantage of near-real-time satellite information, we defined a sampling strategy in order to cross a frontal zone separating different water masses. Multi-parametric in situ sensors mounted on the research vessel, on a towed vehicle and on an ocean glider were used to sample physical and biogeochemical variables at a high spatial resolution. Particular attention was given to adapting the sampling route in order to estimate the vertical velocities in the frontal area also. This strategy was successful in sampling quasi-synoptically an oceanic area characterized by the presence of a narrow front with an associated vertical circulation. A multiparametric statistical analysis of the collected data identifies two water masses characterized by different abundances of several phytoplankton cytometric functional groups, as well as different concentrations of chlorophyll a and O₂. Here, we focus on moderately energetic fronts induced by fine-scale circulation. Moreover, we explore physical–biological coupling in an oligotrophic region. Our results show that the fronts induced by the fine-scale circulation, even if weaker than the fronts occurring in energetic and nutrient-rich boundary current systems, maintain nevertheless a strong structuring effect on the phytoplankton community by segregating different groups at the surface. Since oligotrophic and moderately energetic regions are representative of a very large part of the world ocean, our results may have global significance when extrapolated.

The temporal evolution of a surface chlorophyll a bloom sampled in the western tropical South Pacific during the 2015 Oligotrophy to UlTra-oligotrophy PACific Experiment (OUTPACE) cruise is examined. This region is usually characterized by largely oligotrophic conditions, i.e. low concentrations of inorganic nutrients at the surface and deep chlorophyll a maxima. Therefore, the presence of a surface bloom represents a significant perturbation from the mean ecological state. Combining in situ and remote sensing datasets, we characterize both the bloom's biogeochemical properties and the physical circulation responsible for structuring it. Biogeochemical observations of the bloom document the bloom itself, a subsequent decrease of surface chlorophyll a, significantly reduced surface phosphate concentrations relative to subtropical gyre water farther east, and a physical decoupling of chlorophyll a from a deep nitracline. All these characteristics are consistent with nitrogen fixation occurring within the bloom. The physical data suggest surface mesoscale circulation is the primary mechanism driving the bloom's advection, whereas balanced motions expected at submesoscales provide little contribution to observed flow. Together, the data provide a narrative where subtropical gyre water can produce significant chlorophyll a concentrations at the surface that is stirred, deformed, and transported great distances by the mesoscale circulation. In this case, for the time period considered, the transport is in an easterly direction, contrary to both the large-scale and mean mesoscale flow. As a result, future studies concerning surface production in the region need to take into account the role complex mesoscale structures play in redistributing subtropical gyre water.

The dynamics of the Sicily Channel and the southern Tyrrhenian Sea are highly influenced by the seasonal variability of the Mediterranean basin-wide circulation, by the interannual variability of the numerous mesoscale structures present in the Channel, and by the decadal variability of the adjacent Ionian Sea. In the present study, all these aspects are investigated using in-situ (Lagrangian drifter trajectories and Argo float profiles) and satellite data (Absolute Dynamic Topography, Sea Level Anomaly, Sea Surface Temperature, wind products) over the period from 1993 to 2018. The availability of long time series of data and high-resolution multi-sensor surface currents allow us to add new details on the circulation features and on their driving mechanisms and to detect new permanent eddies not yet described in literature. The structures prevailing in winter are mainly driven by wind, whereas those prevailing in summer are regulated by topographical forcing on surface currents. The strength of the surface structures located at the western entrance of the Ionian Sea and of the mesoscale activity along the northern Sicily coast is modulated by the large-scale internal variability. The vertical hydrological characteristics of these mesoscale eddies are delineated using the Argo float profiles inside these structures.