In order to evaluate the quantity and quality of forage when intercropping forage sorghum (Sorghum bicolor L.) with lathyrus (Lathyrus sativus) and hairy vetch (Vicia villosa), and using different weed management methods such as double cropping, a factorial experiment in a randomized complete block design with three replications was carried out at the research station of the University of Zanjan over two growing seasons (2015 and 2016). In this experiment, the intercropping of forage sorghum with lathyrus and hairy vetch at six levels with single cropping of forage sorghum, lathyrus, and hairy vetch, and three weed management strategies (no weed control, full weed control, and single weed control) was evaluated. The results showed that most forage sorghum traits were significantly (p ≤ 0.05) affected by different sowing ratios. The highest fresh forage yield of sorghum (77.9 ton/ha) and lowest (49.0 ton/ha) were obtained with sorghum + 33% hairy vetch and sorghum + 100% lathyrus, respectively. Forage qualitative traits were also affected by intercropping and weed management. The highest average acid detergent fiber (ADF), neutral detergent fiber (NDF), and total ash percentage (ASH) were obtained with 100% sorghum + 66% lathyrus and 33% hairy vetch. The results showed that sorghum intercropping with 33% lathyrus led to a significant reduction in dry matter intake and relative feed value with no weed control and single weed control. This study demonstrated that, by selecting the appropriate intercropping ratios and forage legumes, we could largely control sorghum weeds in addition to improving the quantitative and qualitative yield of sorghum forage.

The use of growth-stimulating signals to increase the tolerance of plants to water deficits can be an important strategy in the production of plants in dry areas. Therefore, a split-plot experiment with three replications was conducted to evaluate the effects of sodium nitroprusside (SNP) application rate as an NO donor (0, 100, and 200 µM) on the growth and yield parameters of Silybum marianum L. (S. marianum) under different irrigation cut-off times (control, irrigation cut-off from stem elongation, and anthesis). The results of this study showed that with increasing drought severity, leaf RWC, proline content and capitula per plant, 1000 grain weight, plant height, branch per plant, capitula diameter, and the biological and grain yield of S. marianum decreased significantly, whereas the number of grains per capitula increased compared with the control. Also, by irrigation cut-off from the stem elongation stage, the density of leaf stomata at the bottom and top epidermis increased by 64% and 39%, respectively, and the length of the stomata at the bottom epidermis of the leaf decreased up to 28%. In contrast, the results of this experiment showed that the exogenous application of nitric oxide reduced the negative effects of irrigation cut-off, such that the application of 100 µM SNP enhanced RWC content (up to 9%), proline concentration (up to 40%), and grain (up to 34%) and biological (up to 44%) yields in plants under drought stress compared with non-application of SNP. The decrease in the number of capitula per plant and capitula diameter was also compensated by foliar application of 100 µM SNP under stress conditions. In addition, exogenous NO changed the behavior of the stomata during the period of dehydration, such that plants treated with SNP showed a decrease in the stomatal density of the leaf and an increase in the length of the stomata at the leaf bottom epidermis. These results indicate that SNP treatment, especially at 100 µM, was helpful in alleviating the deleterious effects of water deficiency and enhancing the tolerance of S. marianum to withholding irrigation times.