Due to their long-term colonization of and widespread distribution in plateau environments, yaks can serve as an ideal natural animal model for the adaptive evolution of other plateau species, including humans. Some studies reported that the lung and heart are two key organs that show adaptive transcriptional changes in response to high altitudes, and most of the genes that show differential expression in lung tissue across different altitudes display nonlinear regulation. To explore the molecular mechanisms that are activated in yak lung tissue in response to hypoxia, the mRNAs, lncRNAs and miRNAs of lung tissue from 9 yaks living at three different altitudes (3400 m, 4200 m and 5000 m), with three repetitions per altitude, were sequenced. Two Zaosheng cattle from 1500 m were selected as low-altitude control. A total of 21,764 mRNAs, 14,168 lncRNAs and 1209 miRNAs (305 known and 904 novel miRNAs) were identified. In a comparison of yaks and cattle, 4975 mRNAs, 3326 lncRNAs and 75 miRNAs were differentially expressed. A total of 756 mRNAs, 346 lncRNAs and 83 miRNAs were found to be differentially expressed among yaks living at three different altitudes (fold change ≥ 2 and P-value < 0.05). The differentially expressed genes between yaks and cattle were functionally enriched in long-chain fatty acid metabolic process and protein processing, while the differentially expressed genes among yaks living at three different altitudes were enriched in immune response and the cell cycle. Furthermore, competing endogenous RNA (ceRNA) networks were investigated to illustrate the roles of ceRNAs in this process, the result was also support the GO and KEGG analysis. The present research provides important genomic insights for discovering the mechanisms that are activated in response to hypoxia in yak lung tissue.

During the postnatal stages, skeletal muscle development undergoes a series of meticulously regulated alterations in gene expression. However, limited studies have employed chromatin accessibility to unravel the underlying molecular mechanisms governing muscle development in yak species. Therefore, we conducted an analysis of both gene expression levels and chromatin accessibility to comprehensively characterize the dynamic genome-wide chromatin accessibility during muscle growth and development in the Tianzhu white yak, thereby elucidating the features of accessible chromatin regions throughout this process. Initially, we compared the differences in chromatin accessibility between two groups and observed that calves exhibited higher levels of chromatin accessibility compared to adult cattle, particularly within ±2 kb of the transcription start site (TSS). In order to investigate the correlation between alterations in chromatin accessible regions and variations in gene expression levels, we employed a combination of ATAC-seq and RNA-seq techniques, leading to the identification of 18 central transcriptional factors (TFs) and 110 key genes with significant effects. Through further analysis, we successfully identified several TFs, including Sp1, YY1, MyoG, MEF2A and MEF2C, as well as a number of candidate genes (ANKRD2, ANKRD1, BTG2 and LMOD3) which may be closely associated with muscle growth and development. Moreover, we constructed an interactive network program encompassing hub TFs and key genes related to muscle growth and development. This innovative approach provided valuable insights into the molecular mechanism underlying skeletal muscle development in the postnatal stages of Tianzhu white yaks while also establishing a solid theoretical foundation for future research on yak muscle development.

Previous studies have shown that Southeast Asian pigs were independently domesticated from local wild boars. However, the domestication of Chinese native pigs remains a subject of debate. In the present study, phylogenetic analysis of Chinese native pigs was performed by screening for haplotypes inferred from a phylogenetic tree of pig mitochondrial DNA (mtDNA) sequences based on sequence-specific mutations. A total of 2466 domestic pigs formed 124 haplotypes and were assigned to four clades. Clade A comprised pigs distributed mainly in the Qinghai-Tibet Plateau and its surrounding areas; these pigs clustered into three groups. The pigs of clade B were mainly from the Mekong River Basin in Yunnan Province and had been exposed to genetic infiltration from European populations. Clade C comprised pigs mainly from the middle and lower reaches of the Yangtze River. The pigs of clade D were distributed mainly at the intersection of Yunnan, Sichuan, and Gansu provinces east of the Hengduan Mountains (YSGH). Compared with wild boar, at least three domestication centers and one expansion center of pigs in China were detected. Among the four centers detected, two were for Tibetan pigs and were in the Qinghai-Tibet Plateau and at the YSGH intersection, and the other two were in the Mekong River Basin in Yunnan Province and the middle and downstream regions of the Yangtze River.

Quinoa is the only single plant that can meet all the nutritional needs of human, and its potential for feed utilization has been continuously explored, becoming a prosperous industry for poverty alleviation. In order to further tap the feeding value of whole quinoa, develop quinoa as a feed substitute for conventional crops such as corn, and improve its comprehensive utilization rate, this experiment analyzed the silage quality and mycotoxin content of mixed silage of whole-plant quinoa (WPQ) with whole-plant corn (WPC) or stevia powder(SP) in different proportions, and further improved the silage quality of mixed silage by using two lactic acid bacteria preparations (Sila-Max and Sila-Mix). The quality, microbial population, and mycotoxin levels of quinoa and corn silage, as well as that of the mixed silage of quinoa and stevia, were evaluated using single-factor analysis of variance. The impact of various lactic acid bacteria preparations on the quality of whole-quinoa and whole-corn mixed silage was investigated through two-factor analysis of variance. WPQ and WPC were mixed at the ratio of 5:5 (QB5), 6:4 (QB6), 7:3 (QB7), 8:2 (QB8), 9:1 (QB9) and 10:0 (QB10). SP was mixed with WPQ at the supplemental levels of 0.2% (QB10S2), 0.4% (QB10S4), 0.6% (QB10S6), 0.8% (QB10S8) and 1.0% (QB10S10). After 60 days of silage, the silage indexes, the number of harmful microorganisms, and the mycotoxin levels were measured, to explore the appropriate ratio of mixed silage. The membership function analysis showed that the quality of mixed silage of WPQ with SP was better, and the optimal addition amount of SP was 0.6%. The results of Max and Mix on the quality improvement test of WPQ with WPC mixed silage showed that the two lactic acid bacteria formulations increased CP and AA content, and reduced NH₃-N/TN; pH was significantly lower than the control group (p < 0.01), and LA was significantly higher than the control group (p < 0.01). The microbial count results showed that the addition of lactic acid bacteria preparation significantly reduced the number of molds and aerobic bacteria, and the effect of Mix was better than that of Max. When the mixing ratio was between QB7 and QB10, mold was not detected in the lactic-acid-bacteria preparation groups. Max and Mix significantly reduced the levels of mycotoxins, both of which were far below the range of feed safety testing, and 16S rRNA sequencing revealed that the silage microbiota varied with different mixing ratios and whether lactic acid bacteria preparations were used. Max and Mix increased the relative abundance of Firmicutes, with Mix having a more significant effect, especially in the QB6 (65.05%) and QB7 (63.61%) groups. The relative abundance of Lactobacillus was significantly higher than that of the control group (p < 0.05). The relative abundance of Enterobacteriaceae and Streptococcus were negatively and positively correlated with the addition level of quinoa, respectively. Comprehensive analysis showed that adding 0.6% SP to the WPQ and using Mix in mixed silage of WPQ and WPC with the proportion of WPQ no less than 70% had the best silage effect, and was more beneficial to animal health.

The polymorphisms of A-FABP gene which associated with meat quality traits of beef cattle divided in five groups(Qingyang native beef cattle group, Pingliang native beef cattle group, Qinchuan beef cattle group, South Devon beef crossbreed group, Simmental beef crossbreed group) in Gansu was studied. Three types of bands defined as genotypes GG, GC and CC were discovered in the study. According to the results we could find the mutation of c. 408 g > c existed in A- FABP gene’s exon3 area by sequencing analysis of gene in three different kinds. The analysis of A-FABP gene polymorphisms associated with meat quality traits like pressing lose, shear force, cooking loss, marbling score, meat color and pH value, showed that pressing loss with genotype GG was significantly greater than genotype CC (P less than 0.05), shear force with genotype GG was significantly greater than genotype GC and genotype CC (P less than 0.01), cooking loss and pH value with genotype GG were significantly greater than genotype GC (P less than 0.05) and genotype CC (P less than 0.01). This mutation of A-FABP gene could be considered as a locus associated with meat quality traits.