Chestnut plants (Castanea) are important nut fruit trees worldwide. However, little is known regarding the genetic relationship and evolutionary history of different species within the genus. How modern chestnut plants have developed local adaptation to various climates remains a mystery. The genomic data showed that Castanea henryi first diverged in the Oligocene ~31.56 million years ago, followed by Castanea mollissima, and the divergence between Castanea seguinii and Castanea crenata occurred in the mid-Miocene. Over the last 5 million years, the population of chestnut plants has continued to decline. A combination of selective sweep and environmental association studies was applied to investigate the genomic basis of chestnut adaptation to different climates. Twenty-two candidate genes were associated with temperature and precipitation. We also revealed the molecular mechanism by which CmTOE1 interacts with CmZFP8 and CmGIS3 to promote the formation of non-glandular trichomes for adaptation to low temperature and high altitudes. We found a significant expansion of CER1 genes in Chinese chestnut (C. mollissima) and verified the CmERF48 regulation of CmCER1.6 adaptation to drought environments. These results shed light on the East Asian chestnut plants as a monophyletic group that had completed interspecific differentiation in the Miocene, and provided candidate genes for future studies on adaptation to climate change in nut trees.

Aluminum (Al) is an important element in soil constitution. Previous studies have shown that high concentration of Al affects the normal growth of crops, resulting in crop yield reduction and inferior quality. Nevertheless, Al has also been referred to as a beneficial element, especially when used at low concentrations, but the cytological mechanism is not clear. Influences of low concentration AlCl₃ on the pollen tube growth of apple (Malus domestica) and its possible cytological mechanism were investigated in this study. The results showed that 20 μM AlCl₃ promoted pollen germination and tube elongation; 20 μM AlCl₃ enhanced Ca²⁺ influx but did not affect [Ca²⁺]c of the pollen tube tip; and 20 μM AlCl₃ decreased acid pectins in pollen tubes but increased esterified pectins and arabinan pectins in pollen tubes. According to the information provided in this research, 20 μM AlCl₃ stimulated growth of pollen tubes by enhancing Ca²⁺ influx and changing cell wall components.