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The concentrations of inorganic, organic and total carbon, and some sedimentary parameters (sediment density, fines, pH, and shell fragments), have been analyzed in surface sediments of the Venice Lagoon since 1987. Environmental scenarios, characterized by different anthropogenic impacts, have been considered, especially in the central basin where more information is available. Data collected in 2009 in the lagoons and ponds of Po Delta, in Comacchio Valleys and Pialassa della Baiona have been also considered and analyzed together with those recorded in the whole Venice Lagoon in 2011. The results show a strong correlation of the inorganic carbon (Cinorg) with the carbonatic or siliceous origins of the sediments and changes of both Cinorg and organic carbon (Corg) according to different anthropogenic impacts, especially eutrophication and clam-fishing activities. Higher sediment density, grain-size, and pH were associated to good-high ecological conditions and the higher presence of inorganic carbon of biological origin (shell fragments and calcified macroalgal fragments). Conversely, Corg, which is associated to eutrophic conditions, was strongly affected by the sediment disturbance and the presence of high concentrations of bivalves which enhance its consumption.
Adriano Sfriso; Alessandro Buosi; Yari Tomio; Abdul-Salam Juhmani; Stefania Chiesa; Marta Greco; Chiara Gazzola; Michele Mistri; Cristina Munari; Andrea Augusto Sfriso. Sediment Carbon Variations in the Venice Lagoon and Other Transitional Water Systems of the Northern Adriatic Sea. Water 2020, 12, 3430 .
AMA StyleAdriano Sfriso, Alessandro Buosi, Yari Tomio, Abdul-Salam Juhmani, Stefania Chiesa, Marta Greco, Chiara Gazzola, Michele Mistri, Cristina Munari, Andrea Augusto Sfriso. Sediment Carbon Variations in the Venice Lagoon and Other Transitional Water Systems of the Northern Adriatic Sea. Water. 2020; 12 (12):3430.
Chicago/Turabian StyleAdriano Sfriso; Alessandro Buosi; Yari Tomio; Abdul-Salam Juhmani; Stefania Chiesa; Marta Greco; Chiara Gazzola; Michele Mistri; Cristina Munari; Andrea Augusto Sfriso. 2020. "Sediment Carbon Variations in the Venice Lagoon and Other Transitional Water Systems of the Northern Adriatic Sea." Water 12, no. 12: 3430.
The accumulation of Posidonia oceanica dead leaves on the beaches of the Mediterranean shores is a natural phenomenon. They are either temporary or permanent structures (banquettes) and represent a valuable resource, with important ecosystem functions including coastal protection against erosion. Nevertheless, the perception of these plant accumulations by the different stakeholders (beach managers, local administrations and tourists) is often negative; they consider these deposits a malevolent waste to be removed, rather than a natural and valuable component of the coastline. We propose an integrated/beneficial management model for posidonia deposits, called ECOLOGICAL BEACH, firstly proposed in France, and recently implemented and applied in Italy. The model promotes the preservation of posidonia beach casts on site, with a balanced coexistence of natural and anthropic elements. The model fosters the several important ecosystem services of the beach casts and contributes to coastal preservation. To successfully spread the model, several activities must be implemented: a regulatory framework, the collection of data about the occurrence of beach casts, management protocols and educational programs. The most important activity is the educational one, based on the dissemination of the ecological and economic value of the beach casts, aimed at switching the perception of this phenomenon towards positive appraisal.
Alice Rotini; Stefania Chiesa; Loredana Manfra; Patrizia Borrello; Raffaella Piermarini; Cecilia Silvestri; Sergio Cappucci; Luca Parlagreco; Saverio DeVoti; Marco Pisapia; Carla Creo; Tiziana Mezzetti; Alfonso Scarpato; Luciana Migliore. Effectiveness of the “Ecological Beach” Model: Beneficial Management of Posidonia Beach Casts and Banquette. Water 2020, 12, 3238 .
AMA StyleAlice Rotini, Stefania Chiesa, Loredana Manfra, Patrizia Borrello, Raffaella Piermarini, Cecilia Silvestri, Sergio Cappucci, Luca Parlagreco, Saverio DeVoti, Marco Pisapia, Carla Creo, Tiziana Mezzetti, Alfonso Scarpato, Luciana Migliore. Effectiveness of the “Ecological Beach” Model: Beneficial Management of Posidonia Beach Casts and Banquette. Water. 2020; 12 (11):3238.
Chicago/Turabian StyleAlice Rotini; Stefania Chiesa; Loredana Manfra; Patrizia Borrello; Raffaella Piermarini; Cecilia Silvestri; Sergio Cappucci; Luca Parlagreco; Saverio DeVoti; Marco Pisapia; Carla Creo; Tiziana Mezzetti; Alfonso Scarpato; Luciana Migliore. 2020. "Effectiveness of the “Ecological Beach” Model: Beneficial Management of Posidonia Beach Casts and Banquette." Water 12, no. 11: 3238.
In animals, doubly uniparental inheritance (DUI) is a major exception to the common strict maternal inheritance of mitochondria. To date, DUI has only been found in many bivalve species, but its distribution is still unclear. Given the great species richness of the class, much effort is needed to further investigate the occurrence of DUI in unsampled species. A compelling evidence of DUI is generally the presence of a sex‐linked heteroplasmy, where two divergent mitochondrial lineages are found: One is isolated from the male germline, and the other one is isolated from the female germline and, normally, from the soma of both sexes. In the present study, we investigated the sex‐linked heteroplasmy in the razor clam Solen marginatus Pulteney, 1799 and in the lagoon cockle Cerastoderma glaucum (Bruguière, 1789) using two mitochondrial markers (cox1 and rrnL). We found evidence of DUI in the species S. marginatus, with a divergence up to 21% for the rrnL gene, but not in C. glaucum. Moreover, our phylogenetic reconstruction includes all the available data for heterodont species with sex‐linked heteroplasmy and suggests multiple origins of DUI in this subclass, as well as the presence of DUI in other species of the genus Solen.
Livia Lucentini; Federico Plazzi; Andrea Augusto Sfriso; Claudia Pizzirani; Adriano Sfriso; Stefania Chiesa. Additional taxonomic coverage of the doubly uniparental inheritance in bivalves: Evidence of sex‐linked heteroplasmy in the razor clam Solen marginatus Pulteney, 1799, but not in the lagoon cockle Cerastoderma glaucum (Bruguière, 1789). Journal of Zoological Systematics and Evolutionary Research 2020, 58, 561 -570.
AMA StyleLivia Lucentini, Federico Plazzi, Andrea Augusto Sfriso, Claudia Pizzirani, Adriano Sfriso, Stefania Chiesa. Additional taxonomic coverage of the doubly uniparental inheritance in bivalves: Evidence of sex‐linked heteroplasmy in the razor clam Solen marginatus Pulteney, 1799, but not in the lagoon cockle Cerastoderma glaucum (Bruguière, 1789). Journal of Zoological Systematics and Evolutionary Research. 2020; 58 (2):561-570.
Chicago/Turabian StyleLivia Lucentini; Federico Plazzi; Andrea Augusto Sfriso; Claudia Pizzirani; Adriano Sfriso; Stefania Chiesa. 2020. "Additional taxonomic coverage of the doubly uniparental inheritance in bivalves: Evidence of sex‐linked heteroplasmy in the razor clam Solen marginatus Pulteney, 1799, but not in the lagoon cockle Cerastoderma glaucum (Bruguière, 1789)." Journal of Zoological Systematics and Evolutionary Research 58, no. 2: 561-570.
We present a comprehensive review of the available global literature on the genetics and genomics of marine fish invasions. Overall this review provides data from 66 species belonging to 39 families, collected from 80 published studies on both WoS (Web of Science) and Scopus databases. We found that studies on alien fishes focused on specific geographic areas, mainly the Mediterranean region. Furthermore, most studies restricted their approach to describe patterns of cryptic diversity and/or the development of novel markers, whilst the genetic structure of introduced populations and the genetic mechanisms driving the invasion processes were mostly neglected. The majority of studies on marine fishes reveal similar genetic diversity levels in both native and introduced ranges, suggesting massive or multiple introductions. Indeed, cases of introduced populations showing evidence of bottlenecks were rare. Genetic arrangements are not explained by taxonomic group or reproductive strategy but recent studies reveal rapid evolutionary changes associated with invasive lineages, opening new grounds to investigate mechanisms of adaptation in the natural environment. Finally, the potential of marine fish invasions as a model to test evolutionary responses to rapid environmental changes is further discussed.
Stefania Chiesa; Ernesto Azzurro; Giacomo Bernardi. The genetics and genomics of marine fish invasions: a global review. Reviews in Fish Biology and Fisheries 2019, 29, 837 -859.
AMA StyleStefania Chiesa, Ernesto Azzurro, Giacomo Bernardi. The genetics and genomics of marine fish invasions: a global review. Reviews in Fish Biology and Fisheries. 2019; 29 (4):837-859.
Chicago/Turabian StyleStefania Chiesa; Ernesto Azzurro; Giacomo Bernardi. 2019. "The genetics and genomics of marine fish invasions: a global review." Reviews in Fish Biology and Fisheries 29, no. 4: 837-859.
Despite their ecological and economical relevance, a lack of data is still occurring about the distribution, abundance, bioaccumulation and risks for consumption of some edible bivalves. The present study has been carried out in the Venice Lagoon taking into account three bivalve species to investigate i) the possible relationship among the biological features-distribution, abundance and bioaccumulation patterns- of razor clams (Solen marginatus Pulteney, 1799), Manila clams (Ruditapes philippinarum Adams and Reeve, 1850) and lagoon cockles (Cerastoderma glaucum Poiret, 1789) and the hydrological features, namely sediment physico-chemical characteristics and contamination; ii) their specific role as bioindicators of inorganic contamination; iii) the possible risks for human health associated with the consumption of these edible bivalves. Results showed that species distribution and abundance, especially for razor clams, was mainly influenced by environmental conditions and sediment granulometric composition, above metal(loid) contamination. The contamination patterns were different among species, as Manila clam generally showed higher bioaccumulation values for most of the metal(loid)s, whilst lagoon cockles preferentially accumulated Ni. Eventually, a serious concern exists for the human consumption, for all species and investigated sites regarding As. This study will raise attention on the effects of bioaccumulation of inorganic pollutants by edible bivalves and risks for consumers' safety, especially concerning razor clams and cockles, for which a critical lack of data on metal(loid) bioaccumulation occurs from the Northern Adriatic Sea.
Andrea Augusto Sfriso; Stefania Chiesa; Adriano Sfriso; Alessandro Buosi; Lorena Gobbo; Alessia Boscolo Gnolo; Emanuele Argese. Spatial distribution, bioaccumulation profiles and risk for consumption of edible bivalves: a comparison among razor clam, Manila clam and cockles in the Venice Lagoon. Science of The Total Environment 2018, 643, 579 -591.
AMA StyleAndrea Augusto Sfriso, Stefania Chiesa, Adriano Sfriso, Alessandro Buosi, Lorena Gobbo, Alessia Boscolo Gnolo, Emanuele Argese. Spatial distribution, bioaccumulation profiles and risk for consumption of edible bivalves: a comparison among razor clam, Manila clam and cockles in the Venice Lagoon. Science of The Total Environment. 2018; 643 ():579-591.
Chicago/Turabian StyleAndrea Augusto Sfriso; Stefania Chiesa; Adriano Sfriso; Alessandro Buosi; Lorena Gobbo; Alessia Boscolo Gnolo; Emanuele Argese. 2018. "Spatial distribution, bioaccumulation profiles and risk for consumption of edible bivalves: a comparison among razor clam, Manila clam and cockles in the Venice Lagoon." Science of The Total Environment 643, no. : 579-591.
Silvia Breda; Stefania Chiesa; Rosa Freitas; Etelvina Figueira; Francesca Becherini; Lorena Gobbo; Amadeu M.V.M. Soares; Emanuele Argese. Biogeochemical dynamics and bioaccumulation processes in Manila clam: Implications for biodiversity and ecosystem services in the Ria de Aveiro Lagoon. Estuarine, Coastal and Shelf Science 2018, 209, 136 -148.
AMA StyleSilvia Breda, Stefania Chiesa, Rosa Freitas, Etelvina Figueira, Francesca Becherini, Lorena Gobbo, Amadeu M.V.M. Soares, Emanuele Argese. Biogeochemical dynamics and bioaccumulation processes in Manila clam: Implications for biodiversity and ecosystem services in the Ria de Aveiro Lagoon. Estuarine, Coastal and Shelf Science. 2018; 209 ():136-148.
Chicago/Turabian StyleSilvia Breda; Stefania Chiesa; Rosa Freitas; Etelvina Figueira; Francesca Becherini; Lorena Gobbo; Amadeu M.V.M. Soares; Emanuele Argese. 2018. "Biogeochemical dynamics and bioaccumulation processes in Manila clam: Implications for biodiversity and ecosystem services in the Ria de Aveiro Lagoon." Estuarine, Coastal and Shelf Science 209, no. : 136-148.
The Manila clam is emerging as a relevant species for the Portuguese market. The present work was conducted in the Tagus estuary to evaluate 1) the metals and As content in the sediments of the Tagus estuary, especially on those areas subjected to Manila clam harvesting 2) the metals and As content in clams, and the risk associated with their consumption 3) the physiological and biochemical responses of the clam to metals and As contamination, and its possible role as a pollution bioindicator in the estuarine environment. The most contaminated sediments were identified nearby industrial areas, nevertheless clams collected in low contaminated areas showed high metals and As concentrations. The condition index, glycogen content, membrane oxidative damage, biotransformation enzymes and metallothioneins showed consistent responses to metals and As content in clams. Results emphasize the need for the development of a management plan for the species exploitation in the Tagus estuary.
Stefania Chiesa; Paula Chainho; Ângela Almeida; Etelvina Figueira; Amadeu M.V.M. Soares; Rosa Freitas. Metals and As content in sediments and Manila clam Ruditapes philippinarum in the Tagus estuary (Portugal): Impacts and risk for human consumption. Marine Pollution Bulletin 2018, 126, 281 -292.
AMA StyleStefania Chiesa, Paula Chainho, Ângela Almeida, Etelvina Figueira, Amadeu M.V.M. Soares, Rosa Freitas. Metals and As content in sediments and Manila clam Ruditapes philippinarum in the Tagus estuary (Portugal): Impacts and risk for human consumption. Marine Pollution Bulletin. 2018; 126 ():281-292.
Chicago/Turabian StyleStefania Chiesa; Paula Chainho; Ângela Almeida; Etelvina Figueira; Amadeu M.V.M. Soares; Rosa Freitas. 2018. "Metals and As content in sediments and Manila clam Ruditapes philippinarum in the Tagus estuary (Portugal): Impacts and risk for human consumption." Marine Pollution Bulletin 126, no. : 281-292.
The impacts of seawater acidification and salinity shifts on metabolism, energy reserves, and oxidative status of mussels have been largely neglected. With the aim to increase the current knowledge for the mussel Mytilus galloprovincialis a 28-day chronic test was conducted during which mussels were exposed to two pH (7.8 and 7.3; both at control salinity 28) and three salinity (14, 28 and 35, at control pH, 7.8) levels. After exposure to different conditions, mussels electron transport system activity, energy reserves (protein and glycogen content) carbonic anhydrase activity, antioxidant defences and cellular damage were measured. Results obtained showed that mussels exposed to seawater acidification presented decreased metabolic capacity that may have induced lower energy expenditure (observed in higher glycogen, protein and lipids content at this condition). Low pH condition induced the increase of carbonic anhydrase activity that was related to acid-base balance, while no significant activation of antioxidant defence mechanisms was observed resulting in higher LPO. Regarding the impacts of salinity, the present study showed that at the highest salinity (35) mussels presented lower metabolic activity (also related to lower energetic expenditure) and an opposite response was observed at salinity 14. Carbonic anhydrase slightly increased at stressful salinity conditions, a mechanism of homeostasis maintenance. Lower metabolic activity at the highest salinity, probably related to valves closure, helped to mitigate the increase of LPO in this condition. At low salinity (14), despite an increase of antioxidant enzymes activity, LPO increased, probably as a result of ROS overproduction from higher electron transport system activity. The present findings demonstrated that Mytilus galloprovincialis oxidative status and metabolic capacity were negatively affected by low pH and salinity changes, with alterations that may lead to physiological impairments namely on mussels reproductive output, growth performance and resistance to disease, with ecological and economic implications. Indicators: Physiological and biochemical changes in Mytilus galloprovincialis in response to low pH and salinity change
Rosa Freitas; Lucia De Marchi; Miguel Bastos; Anthony Moreira; Cátia Velez; Stefania Chiesa; Frederick J. Wrona; Etelvina Figueira; Amadeu Soares. Effects of seawater acidification and salinity alterations on metabolic, osmoregulation and oxidative stress markers in Mytilus galloprovincialis. Ecological Indicators 2017, 79, 54 -62.
AMA StyleRosa Freitas, Lucia De Marchi, Miguel Bastos, Anthony Moreira, Cátia Velez, Stefania Chiesa, Frederick J. Wrona, Etelvina Figueira, Amadeu Soares. Effects of seawater acidification and salinity alterations on metabolic, osmoregulation and oxidative stress markers in Mytilus galloprovincialis. Ecological Indicators. 2017; 79 ():54-62.
Chicago/Turabian StyleRosa Freitas; Lucia De Marchi; Miguel Bastos; Anthony Moreira; Cátia Velez; Stefania Chiesa; Frederick J. Wrona; Etelvina Figueira; Amadeu Soares. 2017. "Effects of seawater acidification and salinity alterations on metabolic, osmoregulation and oxidative stress markers in Mytilus galloprovincialis." Ecological Indicators 79, no. : 54-62.
The Manila clam Ruditapes philippinarum – synonym Venerupis philippinarum (Adams and Reeve, 1850) is now one of the top 5 most commercially valuable bivalve species worldwide. Originally from the Indo-Pacific region, it has been introduced in many countries for fisheries and aquaculture, including estuarine environments along Atlantic and Mediterranean European coasts. Yet despite its commercial value and widespread distribution, the precise origins of stocks remain speculative and the genetic diversity of introduced populations is poorly known. Thus, the aim of this work was to collect mtDNA COI (Cytochrome oxidase I) gene sequences from 5 European countries with Manila clam stocks and compare them with native Asian populations to evaluate their genetic diversity and identify possible routes of invasion. The COI gene sequencing supported a strong founder effect in the European populations with 3 main haplotypes occurring at high frequencies, derived from Japan. However, high haplotype diversity was also observed due to the occurrence of 10 rare haplotypes. This supports hypotheses (i) there have been additional, previous unrecorded, introductions as previously hypothesized by analysis of 16S rDNA, and (ii) there has been a limited loss of genetic diversity in introduced populations, as previously suggested by microsatellite data. This is the first genetic comparison of Manila clam populations introduced in to Europe with native clams. Genetic data herein presented are fundamentally important for the traceability of clam products and stock management programmes and will also inform discussion on the potential resilience of exploited Manila clam populations.
Stefania Chiesa; Livia Lucentini; Rosa Freitas; Francesco Nonnis Marzano; Silvia Breda; Etelvina Figueira; Nathalie Caill-Milly; Roger J.H. Herbert; Amadeu Soares; Emanuele Argese. A history of invasion: COI phylogeny of Manila clam Ruditapes philippinarum in Europe. Fisheries Research 2016, 186, 25 -35.
AMA StyleStefania Chiesa, Livia Lucentini, Rosa Freitas, Francesco Nonnis Marzano, Silvia Breda, Etelvina Figueira, Nathalie Caill-Milly, Roger J.H. Herbert, Amadeu Soares, Emanuele Argese. A history of invasion: COI phylogeny of Manila clam Ruditapes philippinarum in Europe. Fisheries Research. 2016; 186 ():25-35.
Chicago/Turabian StyleStefania Chiesa; Livia Lucentini; Rosa Freitas; Francesco Nonnis Marzano; Silvia Breda; Etelvina Figueira; Nathalie Caill-Milly; Roger J.H. Herbert; Amadeu Soares; Emanuele Argese. 2016. "A history of invasion: COI phylogeny of Manila clam Ruditapes philippinarum in Europe." Fisheries Research 186, no. : 25-35.
Stefania Chiesa; Livia Lucentini; Rosa Freitas; Francesco Nonnis Marzano; Claudio Ferrari; Laura Filonzi; Silvia Breda; Fabiola Minello; Etelvina Figueira; Emanuele Argese. Null alleles of microsatellites for Manila clam Ruditapes philippinarum. Animal Genetics 2015, 47, 135 -136.
AMA StyleStefania Chiesa, Livia Lucentini, Rosa Freitas, Francesco Nonnis Marzano, Claudio Ferrari, Laura Filonzi, Silvia Breda, Fabiola Minello, Etelvina Figueira, Emanuele Argese. Null alleles of microsatellites for Manila clam Ruditapes philippinarum. Animal Genetics. 2015; 47 (1):135-136.
Chicago/Turabian StyleStefania Chiesa; Livia Lucentini; Rosa Freitas; Francesco Nonnis Marzano; Claudio Ferrari; Laura Filonzi; Silvia Breda; Fabiola Minello; Etelvina Figueira; Emanuele Argese. 2015. "Null alleles of microsatellites for Manila clam Ruditapes philippinarum." Animal Genetics 47, no. 1: 135-136.
Stefania Chiesa; Massimiliano Scalici; Livia Lucentini; Francesco Marzano. Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters. Aquatic Invasions 2015, 10, 209 -216.
AMA StyleStefania Chiesa, Massimiliano Scalici, Livia Lucentini, Francesco Marzano. Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters. Aquatic Invasions. 2015; 10 (2):209-216.
Chicago/Turabian StyleStefania Chiesa; Massimiliano Scalici; Livia Lucentini; Francesco Marzano. 2015. "Molecular identification of an alien temnocephalan crayfish parasite in Italian freshwaters." Aquatic Invasions 10, no. 2: 209-216.
Stefania Chiesa; Livia Lucentini; Rosa Freitas; Francesco Nonnis Marzano; Fabiola Minello; Claudio Ferrari; Laura Filonzi; Etelvina Figueira; Silvia Breda; Gianluca Baccarani; Emanuele Argese. Genetic diversity of introduced Manila clam Ruditapes philippinarum populations inferred by 16S rDNA. Biochemical Systematics and Ecology 2014, 57, 52 -59.
AMA StyleStefania Chiesa, Livia Lucentini, Rosa Freitas, Francesco Nonnis Marzano, Fabiola Minello, Claudio Ferrari, Laura Filonzi, Etelvina Figueira, Silvia Breda, Gianluca Baccarani, Emanuele Argese. Genetic diversity of introduced Manila clam Ruditapes philippinarum populations inferred by 16S rDNA. Biochemical Systematics and Ecology. 2014; 57 ():52-59.
Chicago/Turabian StyleStefania Chiesa; Livia Lucentini; Rosa Freitas; Francesco Nonnis Marzano; Fabiola Minello; Claudio Ferrari; Laura Filonzi; Etelvina Figueira; Silvia Breda; Gianluca Baccarani; Emanuele Argese. 2014. "Genetic diversity of introduced Manila clam Ruditapes philippinarum populations inferred by 16S rDNA." Biochemical Systematics and Ecology 57, no. : 52-59.
Livia Lucentini; Stefania Chiesa; Daniela Giannetto; Laura Pompei; Mauro Natali; Paolo Sala; Pietro Volta; Massimo Lorenzoni; Diego Fontaneto. Integrative taxonomy does not support the occurrence of two species of the Squalius squalus complex (Actinopterygii, Cypriniformes, Cyprinidae) in Italy. Biochemical Systematics and Ecology 2014, 56, 281 -288.
AMA StyleLivia Lucentini, Stefania Chiesa, Daniela Giannetto, Laura Pompei, Mauro Natali, Paolo Sala, Pietro Volta, Massimo Lorenzoni, Diego Fontaneto. Integrative taxonomy does not support the occurrence of two species of the Squalius squalus complex (Actinopterygii, Cypriniformes, Cyprinidae) in Italy. Biochemical Systematics and Ecology. 2014; 56 ():281-288.
Chicago/Turabian StyleLivia Lucentini; Stefania Chiesa; Daniela Giannetto; Laura Pompei; Mauro Natali; Paolo Sala; Pietro Volta; Massimo Lorenzoni; Diego Fontaneto. 2014. "Integrative taxonomy does not support the occurrence of two species of the Squalius squalus complex (Actinopterygii, Cypriniformes, Cyprinidae) in Italy." Biochemical Systematics and Ecology 56, no. : 281-288.
Seven Italian populations of twaite shad Alosa fallax from Northern and Central Italy were investigated to assess genetic diversity by Cytochrome b (Cytb) gene sequencing. The two ecotypes historically referred to landlocked and anadromous populations were investigated for the first time from a genetic point of view, to clarify their phylogenetic relationships. Moreover, results obtained from populations coming from separated Adriatic and Tyrrhenian basins were compared with data assessed in samples of allis shad Alosa alosa from the Atlantic basin. All the Italian samples were recognized at species level as A. fallax, differing for five mutations from A. alosa. The analyses confirmed the occurrence of a single phylogenetic lineage and of a single species within Italian waters, in both landlocked and migratory populations. The minimum spanning network identified six haplotypes for A. fallax and two haplotypes for A. alosa. The neighbour-joining tree and the maximum likelihood on the Cytb gene sequences confirmed two distinct lineages for A. alosa and A. fallax, without evidence of a separation at specific level within the A. fallax group. A weak separation due to incipient population differentiation was detected between anadromous and landlocked Italian populations, supporting the idea of a recent separation. The molecular data herein collected do not support the existence of the already controversial incipient species Alosa agone. Despite this, the two ecotypes could be considered as different management units from a conservation viewpoint.
Stefania Chiesa; Armando Piccinini; Livia Lucentini; Laura Filonzi; Francesco Nonnis Marzano. Genetic data on endangered twaite shad (Clupeidae) assessed in landlocked and anadromous populations: one or more species? Reviews in Fish Biology and Fisheries 2014, 24, 659 -670.
AMA StyleStefania Chiesa, Armando Piccinini, Livia Lucentini, Laura Filonzi, Francesco Nonnis Marzano. Genetic data on endangered twaite shad (Clupeidae) assessed in landlocked and anadromous populations: one or more species? Reviews in Fish Biology and Fisheries. 2014; 24 (2):659-670.
Chicago/Turabian StyleStefania Chiesa; Armando Piccinini; Livia Lucentini; Laura Filonzi; Francesco Nonnis Marzano. 2014. "Genetic data on endangered twaite shad (Clupeidae) assessed in landlocked and anadromous populations: one or more species?" Reviews in Fish Biology and Fisheries 24, no. 2: 659-670.
A fish population of the carp family Cyprinidae with atypical phenotypic characteristics was observed in one of the main catchments of the Pollino National Park, a valuable, protected area in southern Italy. In this area, the Italian roach Rutilus rubilio (Bonaparte, 1837), a native endemic fish of Tyrrhenean regions, has been introduced in sympatric conditions with Squalius squalus (Bonaparte, 1837) and Telestes muticellus (Bonaparte, 1837). A molecular investigation was carried out to assess the genetic identity of the population with a view to conservation. Direct sequencing of a cytochrome b gene fragment was performed based on 30 individuals of cyprinid fish with atypical phenotype, in addition to 30 S. squalus, 10 T. muticellus, and 30 R. rubilio pure individuals collected in different Italian regions, which served as reference samples. Multiple sequence alignments demonstrated that 50% of atypical-cyprinid haplotypes were maternally inherited from either S. squalus or R. rubilio. No contribution by T. muticellus was determined. Our results indicate an intergeneric hybridization event between S. squalus and R. rubilio, as a consequence of trans-introduction activities of alien species.
Stefania Chiesa; Laura Filonzi; Marina Vaghi; Riccardo Papa; Francesco Nonnis Marzano. Molecular Barcoding of an Atypical Cyprinid Population Assessed by Cytochrome b Gene Sequencing. Zoological Science 2013, 30, 408 -414.
AMA StyleStefania Chiesa, Laura Filonzi, Marina Vaghi, Riccardo Papa, Francesco Nonnis Marzano. Molecular Barcoding of an Atypical Cyprinid Population Assessed by Cytochrome b Gene Sequencing. Zoological Science. 2013; 30 (5):408-414.
Chicago/Turabian StyleStefania Chiesa; Laura Filonzi; Marina Vaghi; Riccardo Papa; Francesco Nonnis Marzano. 2013. "Molecular Barcoding of an Atypical Cyprinid Population Assessed by Cytochrome b Gene Sequencing." Zoological Science 30, no. 5: 408-414.
Systematic uncertainties in the crayfish Austropotamobius pallipes are well grounded by the number of species and subspecies described using different approaches, causing scientists to define this taxon as "complex". However, a key task that conservation programmes are facing regarding the recent and drastic decline of European populations, is the coherent systematic classification of this threatened species. Here we present results obtained by coupling mtDNA and genome analysis suggestive of a novel evolutionary framework to explain the relationships among phylogenetic lineages of A. pallipes. The direct sequencing of mtDNA COI gene fragment revealed a strong geographic structure with four distinct haplogroups separated by a range of 5-25 mutations. However, mitochondrial data were not supported by genomic fingerprinting based on 535 AFLP polymorphisms. Nuclear markers showed an unexpected moderate level of genetic differentiation and the absence of any geographic structure. Consequently, this study proposes that the taxonomic hypothesis of a single species of A. pallipes settling the Italian continental waters, is affected by complex evolutionary events. To solve the paradox, we hypothesized an evolutive scenario in which the separation of ancient mtDNA lineages likely occurred before the latest glacial periods. However, the speciation process remained incomplete due to secondary intensive postglacial contacts that forced the mingling of the genomes, and confounds the phylogeographic signature still detectable within mtDNA. Postglacial dispersion and the following demographic events, such as founder effects, drift and bottlenecks, abruptly depleted the local mtDNA variation, and shaped the current genetic population structure of white-clawed crayfish.
S. Chiesa; M. Scalici; R. Negrini; G. Gibertini; F. Nonnis Marzano. Fine-scale genetic structure, phylogeny and systematics of threatened crayfish species complex. Molecular Phylogenetics and Evolution 2011, 61, 1 -11.
AMA StyleS. Chiesa, M. Scalici, R. Negrini, G. Gibertini, F. Nonnis Marzano. Fine-scale genetic structure, phylogeny and systematics of threatened crayfish species complex. Molecular Phylogenetics and Evolution. 2011; 61 (1):1-11.
Chicago/Turabian StyleS. Chiesa; M. Scalici; R. Negrini; G. Gibertini; F. Nonnis Marzano. 2011. "Fine-scale genetic structure, phylogeny and systematics of threatened crayfish species complex." Molecular Phylogenetics and Evolution 61, no. 1: 1-11.
The coastal lagoons of the Northern Adriatic Sea are among the most worldwide productive locations of Manila clam Ruditapes philippinarum. Although introduced in Italy in 1983 from the Indo-Pacific, fishing and exploitation of Manila clam improved during the years as Italy became the leading country in Europe for production of this shellfish. Despite its commercial importance, genetic structure of R. philippinarum in Northern Adriatic Sea has not been previously investigated. Here we present the first genetic study on Manila clam populations inhabiting a Mediterranean area, assessed by both mitochondrial (16S rDNA) and nuclear DNA (microsatellite loci). Our study showed that this species has a limited genetic differentiation at the mitochondrial level, but a higher rate of genetic diversity can be identified by polymorphic markers as microsatellites. In particular, out of 28 alleles, 7 private ones were recorded for the Venice Lagoon populations, 2 for those of Scardovari and one for the Po River Delta populations. These molecular markers suggest the occurrence of at least two different introduction events from different recruitment stocks, representing a powerful tool not only to assess genetic diversity of an introduced species, but also helpful information to manage aquaculture and fishery stocks, and to warrant food quality, safety and for the authentication of shellfish products, and traceabilty path.
Stefania Chiesa; Francesco Nonnis Marzano; Giovanni Minervini; Davide De Lucrezia; Gianluca Baccarani; Guido Bordignon; Irene Poli; Giampietro Ravagnan; Emanuele Argese. The invasive Manila clam Ruditapes philippinarum (Adams and Reeve, 1850) in Northern Adriatic Sea: Population genetics assessed by an integrated molecular approach. Fisheries Research 2011, 110, 259 -267.
AMA StyleStefania Chiesa, Francesco Nonnis Marzano, Giovanni Minervini, Davide De Lucrezia, Gianluca Baccarani, Guido Bordignon, Irene Poli, Giampietro Ravagnan, Emanuele Argese. The invasive Manila clam Ruditapes philippinarum (Adams and Reeve, 1850) in Northern Adriatic Sea: Population genetics assessed by an integrated molecular approach. Fisheries Research. 2011; 110 (2):259-267.
Chicago/Turabian StyleStefania Chiesa; Francesco Nonnis Marzano; Giovanni Minervini; Davide De Lucrezia; Gianluca Baccarani; Guido Bordignon; Irene Poli; Giampietro Ravagnan; Emanuele Argese. 2011. "The invasive Manila clam Ruditapes philippinarum (Adams and Reeve, 1850) in Northern Adriatic Sea: Population genetics assessed by an integrated molecular approach." Fisheries Research 110, no. 2: 259-267.
In this work, we present molecular barcoding results obtained in 69 processed fish products belonging to 27 teleost species traded in Italian commercial markets during 2008. DNA barcoding using direct sequencing of about 900 bp of mitochondrial genes cytochrome oxidase subunit I (COI) and cytochrome b (Cyt b) revealed uncorrect labelling in 22 samples (32%). Among substituted species, 18 (26%) were serious frauds under both economic and nutritional points of view. In some cases, frauds concerned species of major conservation regard and reported in the IUCN and CITES directories. Results add further concern on the trading of processed fish products in Italy from both health and conservation points of view.
Laura Filonzi; Stefania Chiesa; Marina Vaghi; Francesco Nonnis Marzano. Molecular barcoding reveals mislabelling of commercial fish products in Italy. Food Research International 2010, 43, 1383 -1388.
AMA StyleLaura Filonzi, Stefania Chiesa, Marina Vaghi, Francesco Nonnis Marzano. Molecular barcoding reveals mislabelling of commercial fish products in Italy. Food Research International. 2010; 43 (5):1383-1388.
Chicago/Turabian StyleLaura Filonzi; Stefania Chiesa; Marina Vaghi; Francesco Nonnis Marzano. 2010. "Molecular barcoding reveals mislabelling of commercial fish products in Italy." Food Research International 43, no. 5: 1383-1388.
In 2006 an unusual find of Procambarus clarkii, today considered one of the most dangerous exotic crayfish, was made within the preserved brackish Mediterranean biotope “Palude di Torre Flavia” (Rome, Italy). Here the investigation on the population structure and dynamics of P. clarkii inhabiting this brackish wetland is presented in an attempt to describe the life history pattern in this type of habitat. The present study showed that this species has a lower growth rate and higher longevity than other studied European P. clarkii populations, and that it can damage brackish habitats and human livelihoods. The main results of this study chiefly concern the differences found between several life-history parameters of the studied population and those obtained in previous studies. These results showed the high ecological plasticity of P. clarkii, indicate the need to intervene before the species spreads further in brackish water, for the conservation of Mediterranean coastal habitats.
Massimiliano Scalici; Stefania Chiesa; Stefano Scuderi; Deborah Celauro; Giancarlo Gibertini. Population structure and dynamics of Procambarus clarkii (Girard, 1852) in a Mediterranean brackish wetland (Central Italy). Biological Invasions 2009, 12, 1415 -1425.
AMA StyleMassimiliano Scalici, Stefania Chiesa, Stefano Scuderi, Deborah Celauro, Giancarlo Gibertini. Population structure and dynamics of Procambarus clarkii (Girard, 1852) in a Mediterranean brackish wetland (Central Italy). Biological Invasions. 2009; 12 (5):1415-1425.
Chicago/Turabian StyleMassimiliano Scalici; Stefania Chiesa; Stefano Scuderi; Deborah Celauro; Giancarlo Gibertini. 2009. "Population structure and dynamics of Procambarus clarkii (Girard, 1852) in a Mediterranean brackish wetland (Central Italy)." Biological Invasions 12, no. 5: 1415-1425.
Biological invasions inflict damage to the ecology, economy, and human health, and pose serious threats to the native communities. Among the many invasive taxa, crayfish have attracted much attention by scientists and policy makers. Recently, an established population of an alien species of crayfish, new for Italy, was found in the Natural Preserve of “Laghi di Ninfa” (central Italy). Based on morphological and genetic evidence (molecular barcoding of COI and 16S rDNA), we classified it at subspecies level as the Parastacidae Cherax destructor destructor, native to Australia. Its introduction possibly occurred at the end of the 1980s but the species seems to be still confined in the preserve. The low temperature of the adjacent waters may be a barrier against its natural spreading but not against its intentional translocation into natural waters by man. Because of the invasive history of C. destructor, eradication of this population is urgent and still economically profitable.
Massimiliano Scalici; Stefania Chiesa; Francesca Gherardi; Marina Ruffini; Giancarlo Gibertini; Francesco Nonnis Marzano. The new threat to Italian inland waters from the alien crayfish “gang”: the Australian Cherax destructor Clark, 1936. Hydrobiologia 2009, 632, 341 -345.
AMA StyleMassimiliano Scalici, Stefania Chiesa, Francesca Gherardi, Marina Ruffini, Giancarlo Gibertini, Francesco Nonnis Marzano. The new threat to Italian inland waters from the alien crayfish “gang”: the Australian Cherax destructor Clark, 1936. Hydrobiologia. 2009; 632 (1):341-345.
Chicago/Turabian StyleMassimiliano Scalici; Stefania Chiesa; Francesca Gherardi; Marina Ruffini; Giancarlo Gibertini; Francesco Nonnis Marzano. 2009. "The new threat to Italian inland waters from the alien crayfish “gang”: the Australian Cherax destructor Clark, 1936." Hydrobiologia 632, no. 1: 341-345.