This study evaluated rooting of Pennisetum ‘Vertigo’ cuttings and development and the nutritional status of rooted plants. Cuttings of Pennisetum ‘Vertigo’ rooted in a perlite or peat medium were treated with Goëmar Goteo biostimulator as follows: (1) soaking (2) watering (3) spraying (4) no Goteo applied (control). Then, 83.3–100% of cuttings formed adventitious roots. The, 100% rooting was obtained for plants in perlite when Goteo spraying or watering was used for plants rooted in peat only after Goteo watering application. Cuttings rooted in perlite had 30% more roots and they were longer than in peat. Goteo watering of cuttings affected root elongation in both peat and perlite. Neither rooting media nor biostimulator treatment affected root dry weight (DW). Rooting medium after 2 months of pot cultivation had no effect on biometric features of plants, but those grown from cuttings rooted in peat had a higher fresh weight (FW) compared to those rooted in perlite. Plants developed from Goteo-treated cuttings were higher compared to the control plants. Goteo watering during rooting stimulated the formation of new shoots in the greenhouse cultivation. Plants from cuttings rooted in perlite had more Fe and Cu in their leaves, especially when they were Goteo-watered. Goteo increased P content in plants derived from biostimulator-watered cuttings, and K in plants from cuttings soaked in Goteo and rooted in perlite.
Geophytes are an increasingly widely used group of plants. Its high biodiversity, however, significantly impacts the cultivation process. Therefore, every new insight into the behavioral aspects of each single species is crucial for production. This study, for the first time, aims at contributing to a better understanding of the interplay between the bulb size potential of Narcissus poeticus and the pattern of bulb lifting time to find the optimal combination of these factors in obtaining the best quality propagation material. Twelve bulb size groups were examined. Plants were planted in the open air in two locations of Central East Europe and lifted in three terms: immediately and two and four weeks after flowering. Bulb fresh and dry weight, as well as leaf, root and total plant dry weight, increased with growing bulb size and delayed the time of lifting. The bulb weight increase indicator was twice as high for bulbs lifted in the third date compared to those lifted earlier. Its value was the highest for the smallest bulbs. Bulb shape ratio gradually decreased together with the increase of bulb weight and later lifting term. Principal components analysis allowed singling out two principal components that accounted for 86.58% of total variability.
Micropropagation via organogenesis of Lachenalia viridiflora W. F. Barker, a native endangered plant from South Africa, is presented in this study. Leaves, collected from in vivo plants, were a source for culture initiation to obtain three types of explants (leaf, bulb, callus). They were grown on Murashige and Skoog (MS) medium supplemented with two concentrations of sucrose (3 or 6%, marked as S3 and S6) and different concentrations of benzylaminopurine (BA) (0.0–5.0 μM) and naphthalene acetic acid (NAA) (0.0–0.5 μM). The results demonstrated that bulbing was more efficient on media with 3% sucrose and this process was closely related to explant type—irrespective of media type twice more adventitious bulbs (5.1–5.6) were regenerated on leaf and bulb explants than on callus (2.6). The highest value of adventitious bulb number was recorded for interaction: leaf × S3/2.5 BA/0.5 NAA (9.3 bulbs per explant). Irrespective of media type, the higher the number of adventitious bulbs (obtained from leaf and bulb explants), the lower their diameter. Moreover, adventitious bulbs regenerated from bulb explants formed the longest leaves (18.9 mm) and in the greatest percentage (52.3%). The highest biomass weight increase indicator (50 times) was recorded for interaction: leaf × S6/2.5 BA/0.25 NAA. Various combinations of plant growth regulators favor biomass increase.
The genus Lachenalia (Asparagaceae) represents spectacular and botanically diverse ornamental, bulbous species originating from Southern Africa. The main aim of this study was to determine, for the first time, the possibility of propagation of lachenalia cultivars (‘Namakwa’, ‘Rainbow Bells’, ‘Rupert’) by two in vivo techniques: chipping and scoring, which can be applied in procedures of bulb production. In the second part of the experiment, the plant growth and the quality of bulblets after the first season of cultivation in a greenhouse were estimated. The survival ability of bulb-scale segments and scored bulbs of the tested cultivars ranged from 62% to 95%. Chipping method generated eight bulblets per mother bulb with an average weight and diameter of 0.17 g and 0.29 cm, respectively, while scoring two/three bulblets per mother bulb with an average weight and diameter of 0.28 g and 0.5 cm, respectively. In the first season of cultivation, bulblets obtained by chipping produced longer leaves than those obtained by scoring technique. Only bulblets of ‘Rupert’ obtained by scoring showed the capacity to flower (‘Namakwa’ and ‘Rainbow Bells’ remained in a juvenile phase). When assessing the bulb yield after the first season of cultivation, it was found that irrespective of cultivar, bulbs obtained by chipping achieved twice the weight coefficient of those obtained by scoring.
22 February 2019 in Acta Scientiarum Polonorum Hortorum Cultus
Lachenalia is a poorly known genus of ornamental bulbous plants from South Africa with a huge floricultural potential. The paper discusses in vivo multiplication with leaf cuttings of Lachenalia cv. ‘Namakwa’, ‘Romaud’, ‘Ronina’ and ‘Rupert’. Mature leaves were collected from non-flowering donor plants. Each leaf was halved to make proximal and distal leaf cuttings. ‘Ronina’ distal leaf cuttings produced the lowest number of bulblets (5.7 per cutting), while ‘Namakwa’ proximal leaf cuttings turned out the most productive (10.5 bulblets per cutting). The bulblets produced by cv. ‘Namakwa’ distal leaf cuttings were the lightest (0.3 g per bulblet), and those formed on cv. ‘Ronina’ proximal leaf cuttings were the heaviest (0.9 g per bulblet). In general, the weight of individual bulblets ranged from 0.1 to 1.2 g. Irrespective of the cutting type, the leaves of cv. ‘Namakwa’ produced the greatest number of bulblets, and the leaves of ‘Ronina’ yielded the heaviest bulblets. With no dependence on their genotype, proximal leaf cuttings were advantageous in terms of total number of generated bulblets and their quality (weight, diameter).
The Narcissus poeticus (L.) species stands out from other narcissus due to its unique ornamental and sensory values. In our experiment, the flowers of N. poeticus were harvested at five different stages. After cutting, the plants were placed directly at room temperature or at 4 °C for 1 week. The opening status of the flowers was recorded every 12 h, and based on this, the ornamental value of the flowers was calculated. Additionally, the flower diameter, the scape and flower weight, the dry matter content, the water soluble K, Ca, and Mg content of the plant parts, and the chlorophyll content of the scapes were measured. The complete senescence of the stored flowers was noted to be earlier (after 6.6–7.5 days) than that of the fresh flowers (7.5–8.5 days) and depended on the bud stage at harvest. Flowers opened from all the buds, but the flower size was smaller in the early developmental harvest stage (a 10 mm reduction in diameter and a 0.38 g reduction in fresh weight between the green bud and the large white bud harvest stages). Cold storage delayed further bud development and reduced the flower size in several cases, but it increased the maximum decorative value of the flowers for those flowers that had been harvested as big white buds.