Fourier transform infrared (FTIR) spectroscopy has been extensively used in microplastic (MP) pollution research since 2004. The aim of this review is to discuss and highlight the recent advances in FTIR (spectroscopy and chemical imaging) techniques that are used to characterize various polymer types of MPs and to trace their fate and transport in different environmental matrices. More than 400 research papers dealing with FTIR techniques in MP pollution research, which are published between January 2010 and December 2019, have been identified from the Scopus and Web of Science databases. The MPs present in sediment, water (marine and freshwater), biota, air/dust, waste water treatment plants and salt are further classified according to (1) characterization and identification, (2) weathering and aging, (3) ecotoxicology, and (4) analytical methods. The results revealed that the ATR-FTIR technique is mostly used to identify and characterize the MPs found in water and sediment. The µFTIR (FTIR imaging) is extensively used to study the ingestion of MPs in biota (both marine and freshwater). In this article, we have summarized the current knowledge of application of FTIR spectroscopy to MP research and provided insights to future challenges for understanding the risk of MPs.