Background: Open pit antimony (Sb) mining causes serious soil pollution, and phytoremediation is a low-cost approach to remediate heavy metal contaminated soil. Rhizosphere bacteria play an important role in ecological restoration in mining areas. There is a knowledge gap on how to find suitable rhizosphere microorganisms to improve the phytoremediation effect. Understanding the differences of rhizosphere bacterial diversity in different restoration stages is helpful to find suitable bacteria for ecological restoration. Methods: A method of the substitution of “space” for “time” was used to study the effect of natural restoration on rhizosphere bacterial community. According to the dominant vegetation types (herb, shrub, and tree) in the natural restoration area of Sb mining, the early restoration (ER), middle restoration (MR), and later restoration (LR) from the largest Sb mine (Xikuangshan mine) in the world were selected to evaluate the differences in the composition and diversity of rhizosphere bacteria during three natural restoration stages. Each restoration stage had five samples. To determine the relationship between restoration stages and bacterial diversity in the rhizosphere, high throughput sequencing of PCR amplified were used. Results: Alpha diversity, as assessed by Chao indices, appeared lowest in ER but this trend was not seen with other diversity metrics, including the Simpson and Shannon. Beta diversity analysis suggested there were differences in rhizobacterial community structure associate with restoration stage. At the phylum level, natural restoration led to a significant increase in the relative abundance of Actinobacteria in the MR, and a significant decrease in the relative abundance of Patescibacteria in the LR. Additionally, Calditrichaeota, Deferribacteres and Epsilonbacteraeota were only found in ER. At the genus level, the relative abundance of RB41 and Haliangium were highest in LR plots, while that of Bacillus and Gaiella were highest in ER plots. Additionally, the Azorhizobium genus was only detected in the ER phase. Overall, our findings suggested that several rhizosphere microbial communities had significant differences among three natural restoration stages (ER, MR, and LR) and the rhizosphere bacterial communities mainly appeared in the early restoration stage can be preferred for remediation of pollution soil in Xikuangshan.

The forest community structure and dynamic structure provide basic data for in-depth study of the community maintenance mechanisms and succession processes of urban forest ecosystems. Moreover, the study on the dynamics of suburban forest communities can provide a reference for the succession process of forest communities under severe human disturbance. In this context, this study analyzes the species composition and community structure of a 6 ha subtropical secondary evergreen broad-leaved forest plot in Wuchaoshan National Forest Park, Hangzhou, Eastern China. There are 36,757 existing woody plants (with DBH ≥ 1 cm), belonging to 94 species (45 evergreen species, and 48 deciduous species) in 63 genera of 34 families, with a plant density of 6126 plants/ha. The overall size–class distribution shows an obvious inverted “J” shape. Species with the top-five important values in the sample are Schima superba, Camellia fraternal, Symplocos anomala, Cyclobalanopsis glauca, Eurya rubiginosa, accounting for 57.3% of all individuals within the sample. In addition, different species have different habitat preferences and different distribution areas. The Wuchaoshan forest is in the middle and late stages of the succession. Different interference histories in the community succession process are demonstrated through studies of secondary evergreen broad-leaved forests. Long-term community monitoring contributes to indicating the direction and potential of the secondary forest succession.

The protection and propagation of rare and endangered species are key to the preservation of their population development; however, due to the scarcity of individuals, the potential effects and status of rare and endangered species in the whole forest ecosystem are still poorly understood. Using data from a 60 × 140 m forest dynamic monitoring sample of the Carpinus tientaiensis (Betulaceae) species in Zhejiang of Southeast China. We assessed the population distribution and diameter at breast height (DBH) structure of the Carpinus tientaiensis species, which was a rare and endangered species, as well as intra- and interspecific correlation with other species. The results show that saplings (1 cm ≤ DBH < 5 cm) and juveniles (5 cm ≤ DBH < 10 cm) were more aggregated than larger individuals (DBH ≥ 20 cm) of Carpinus tientaiensis. The DBH size structure of all the trees shows an obvious inverted “J” distribution. With an increase in the DBH size category, the number of individuals gradually decreases. Due to the diffusion limitation, the spatial distribution patterns of all the tree individuals and roof geese in the sample land are increased at a small spatial scale, and as the spatial scale increases, the degree of aggregation decreases gradually. The relationship between different diameter stages of the population of Carpinus tientaiensis showed a consistent general trend. The spatial distribution of individuals with a large diameter on a small scale was significantly positively correlated (p < 0.001). With an increase in the scale, there was no significant correlation (p > 0.05) between individuals with a large diameter and individuals with a small diameter. There was no significant correlation (p > 0.05) between the population of Carpinus tientaiensis and other species in the sample, and the strong unidirectional competition of other species in the sample can be seen by the competition index. We found that interspecific competition restricts the growth and expansion of Carpinus tientaiensis, and it has adopted different ecological strategies to coexist with a population of common tree species occupying a similar living space.

Plant interactions have long been the subject of intense research and debate in ecology. Competition and facilitation are known to be the basic drivers of community structure, species coexistence, and forest succession dynamics; however, there remains a lack of understanding of how spatial patterns affect these in the mixed forest community of coniferous and broad-leaved species. In the present study, we studied the roles of competition and facilitation in a mixed forest community in the Zhejiang Province, China. For this, we divided plants measured at a study site according to the size of the diameter at breast height (DBH) into three levels of saplings as follows: saplings (1 cm ≤ DBH < 5 cm), juveniles (5 cm ≤ DBH < 15 cm), and adults (DBH ≥ 15 cm). We used the spatial point pattern method to explore the raw number, spatial distribution, and both intra- and inter-specific correlations of coniferous and broad-leaved species at these different diameter levels. The corresponding findings were as follows. First, the DBH and structure of coniferous and broad-leaved species were similar, showing a distinct distribution in an inverted “J” shape as the number of individuals with a particular diameter declined as DBH increased. Second, when all individuals of a similar diameter range were pooled, coniferous species were clustered at all size scales; however, in this situation, broad-leaved species (evergreen and deciduous) showed a clumped distribution at small scales, and this decreased as spatial scale increased. Third, there were small, but significant positive correlations between large-diameter trees and small-diameter trees of coniferous species and between evergreen broad-leaved species at different diameter levels; however, as the scale increased, the correlation diminished. On any scale, individual deciduous broad-leaved species at different diameter ranges did not have any significant correlation. Fourth, coniferous species had a strong competitive effect on broad-leaved species, and there was a weak promoting effect between deciduous and evergreen broad-leaved species as well. In summary, different tree species occupy similar living spaces, and through competition and facilitation, regulate the spatial pattern and community stability of coniferous and broad-leaved species in mixed forest communities.

Negative densification affects the spatial distribution of species in secondary evergreen broad-leaved forests and is a key mechanism governing species coexistence. We investigated the effects of habitat heterogeneity and density on the spatial distribution of populations of dominant woody species in a secondary evergreen broad-leaved forest in Wuchaoshan using spatial univariate point pattern analyses. This 6 ha forest dynamic monitoring sample area in Hangzhou, China is a typical secondary subtropical evergreen broad-leaved forest. We found (1) a strong effect of habitat heterogeneity that led to the spatial aggregation of dominant species in the plot. Habitat heterogeneity had a strong impact on mature individuals at different life history stages and of different species on a large scale. (2) Negative density dependence (NDD) generally affected spatial distributions of most dominant species and decreased in magnitude with age class. Therefore, different species of subtropical evergreen broad-leaved forests in China have formed unique spatial structures due to their habitat preferences but are generally subjected to density-dependent effects.