The influence of nanoparticles (NPs) on aquatic environments is still poorly documented. The aim of the study was to determine the effects of silver (AgNPs) and copper (CuNPs) nanoparticles on larval Siberian sturgeon (Acipenser baerii) after 21 days of exposure. Acute toxicity of AgNPs on Siberian sturgeon was investigated in a 96-h static renewal study and compared with the toxicity of CuNPs. The AgNPs and CuNPs 96 h mean lethal concentrations (96 h LC50) were 15.03 ± 2.91 and 1.41 ± 0.24 mg L⁻¹, respectively. Toxicity tests were done in triplicates for each concentration of AgNPs 0.1, 0.5, 1.5 mg L⁻¹ and CuNPs 0.01, 0.05, 0.15 mg L⁻¹. The control group was exposed in freshwater. The results indicate that AgNPs and CuNPs exposure negatively influenced survival; body length and mass; and morphology and physiology of the epidermis, gills, and liver of Siberian sturgeon larvae. Fish exposed to AgNPs and CuNPs showed similar pathological changes: irregular structure and pyknotic nuclei of epidermis, aplasia and/or fusion of lamellae, telangiectasis, epithelial necrosis and lifting of the gills, dilation of sinusoidal space, overfilled blood vessels, and pyknotic nuclei of the liver. Fish exposed to CuNPs only demonstrated hyaline degeneration in the gills epithelium and liver. The study shows that CuNPs were more toxic to Siberian sturgeon larvae than AgNPs.

Nanoparticles are commonly used in the industry and are present in consumer goods; therefore, evaluation of their potential toxicity is necessary. The aim of the present study was to assess the cytotoxic effects of the nanoparticles of silver (AgNPs) at the concentration of 1.5 mg L⁻¹ and copper (CuNPs) at 0.15 mg L⁻¹ on rainbow trout (Oncorhynchus mykiss) hepatocytes after 28 days of exposure. Histological analysis revealed dilated sinusoids, shrunken hepatocytes, nuclear necrosis, and increased number of Kupffer cells in the liver of fish exposed to nanoparticles. The lowest hepatocyte proliferation index was observed in fish treated with AgNPs. Ultrastructural studies revealed mitochondrial edema and cristolysis, dilated and loosened endoplasmic reticulum, cytoplasm vacuolation, accumulation of lipid droplets, glycogen depletion, and formation of myelin-like bodies. The results also revealed that the liver of fish exposed to copper nanoparticles showed higher regenerative potential indicated by higher proliferation index, more abundant glycogen, and more numerous Kupffer cells compared to the fish treated with silver nanoparticles.

Platinum nanoparticles (NP-Pt) are noble metal nanoparticles with unique physiochemical properties that have recently elicited much interest in medical research. However, we still know little about their toxicity and influence on general health. We investigated effects of NP-Pt on the growth and development of the chicken embryo model with emphasis on brain tissue micro- and ultrastructure. The embryos were administered solutions of NP-Pt injected in ovo at concentrations from 1 to 20 μg/ml. The results demonstrate that NP-Pt did not affect the growth and development of the embryos; however, they induced apoptosis and decreased the number of proliferating cells in the brain tissue. These preliminary results indicate that properties of NP-Pt might be utilized in brain cancer therapy, but potential toxic side effects must be elucidated in extensive follow-up research.

Antidepressants have been detected in surface waters worldwide at ng-μg/L concentration. These compounds can exert adverse effects on fish even at low levels. But, all previous analyses have concentrated on adult fish. The aim of the study was to assess the effect of environmental concentrations of sertraline, paroxetine, fluoxetine and mianserin, and their mixtures on such unusual endpoints as physiological and histological changes of zebrafish (Danio rerio) larvae. We also determined the bioconcentration of the pharmaceuticals. Fish Embryo Toxicity test was used to analyze the influence on developmental progression. Histological sections were stained with hematoxylin and eosin. Proliferating cells in liver were determined immunohistochemically by detection of Proliferating Cell Nuclear Antigens. The bioconcentration factor was measured by liquid chromatography coupled to mass spectrometry. Pharmaceuticals were used at low, medium and high concentrations in mixtures and at medium concentration as single compound. Exposure to the analyzed pharmaceuticals increased the rate of abnormal embryo and larvae development, accelerated the hatching time and affected the total hatching rate. Three-times lower proliferation of hepatocytes was observed in larvae exposed to paroxetine, mianserin, sertraline and the mixture of the pharmaceuticals at the highest concentrations. The highest bioaccumulation factor (BCF) was obtained for sertraline. The BCF of the analyzed compounds was higher if the organisms were exposed to the mixtures than to single pharmaceuticals. To conclude, the exposure of zebrafish larvae to selected antidepressants and their mixtures may cause disturbances in the organogenesis of fish even at environmental concentrations.