Hichem KICHOU is currently PhD student at the EA 6295 NanoMedicine & NanoProbes, Faculty of Pharmacy, University of Tours, France. His research aims to develop a multi-methodological analytical approach providing a comprehensive evaluation of interaction and diffusion of actives ingredients (cosmetic, pharmaceutical) in human skin. Chromatography techniques are preferred to establish kinetics of penetration of molecules trough the different layers of the skin while Raman confocal imaging (RCI) coupled to multivariate analysis is proposed to investigate penetration profiles in stratum corneum and epidermis.