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Silvio S. Silva
Department of Biotechnology, Engineering School of Lorena, University of São Paulo, São Paulo 12602-810, Brazil

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Journal article
Published: 29 August 2021 in Sustainability
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Lasiodiplodan is a β-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 °C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL−1 of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg−1 glucose consumed and 0.016 gL−1 h−1, respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a β-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan.

ACS Style

Peyman Abdeshahian; Jesús Jiménez Ascencio; Rafael R. Philippini; Felipe Antonio Fernandes Antunes; Avinash P. Ingle; Mojgan Abdeshahian; Júlio César dos Santos; Silvio Silvério da Silva. Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw. Sustainability 2021, 13, 9697 .

AMA Style

Peyman Abdeshahian, Jesús Jiménez Ascencio, Rafael R. Philippini, Felipe Antonio Fernandes Antunes, Avinash P. Ingle, Mojgan Abdeshahian, Júlio César dos Santos, Silvio Silvério da Silva. Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw. Sustainability. 2021; 13 (17):9697.

Chicago/Turabian Style

Peyman Abdeshahian; Jesús Jiménez Ascencio; Rafael R. Philippini; Felipe Antonio Fernandes Antunes; Avinash P. Ingle; Mojgan Abdeshahian; Júlio César dos Santos; Silvio Silvério da Silva. 2021. "Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw." Sustainability 13, no. 17: 9697.

Article
Published: 08 July 2021 in BioEnergy Research
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The production of second-generation (2G) ethanol remains an interesting proposition for the implementation of sustainable and net carbon–neutral energy systems. To be economically viable, 2G biorefineries must make use of all processing streams, including the less desirable pentose (C5) sugar stream. In this work, a strategy of sequential dilute acid and alkaline pretreatment of the lignocellulosic feedstock, switchgrass, was implemented for improving the fermentable sugar yield. The hemicellulose-enriched hydrolysate obtained after dilute acid pretreatment was fermented by a newly isolated wild Scheffersomyces parashehatae strain—UFMG-HM-60.1b; the corresponding ethanol yield (YPS) and volumetric productivity (QP) were 0.19 g/g and 0.16 g/L h, respectively. The remaining switchgrass cellulignin fraction was subjected to optimized alkaline delignification at 152 ºC for 30 min. Then, the delignified solid fraction was subjected to contiguous enzymatic saccharification and fermentation releasing a glucose (C6) sugar stream. The control yeast strain, Saccharomyces cerevisiae 174, displayed an ethanol YPS of 0.46 g/g and QP of 0.70 g/L h for the C6 sugar stream, whereas the above-mentioned wild strain presented YPS and QP of 0.29 g/g and 0.38 g/L h, respectively. Upon combining the conversion of hemicellulose (37%) and cellulose-derived sugars (57%), the wild S. parashehatae strain provided higher yield (94%) than the generic S. cerevisiae (90%). Henceforth, our sequential two-stage pretreatment and fermentation of C5 and C6 sugar streams provides a pathway for maximum utilization of switchgrass carbohydrates for 2G ethanol production.

ACS Style

Felipe Antonio Fernandes Antunes; Kalavathy Rajan; ANGELE Djioleu; Thiago Moura Rocha; Larissa Pereira Brumano; Yasmin Cristhine De Souza Melo; Júlio César dos Santos; Carlos A. Rosa; Danielle Julie Carrier; Silvio Silvério da Silva. Sustainable Second-Generation Ethanol Production from Switchgrass Biomass via Co-fermentation of Pentoses and Hexoses Using Novel Wild Yeasts. BioEnergy Research 2021, 1 -12.

AMA Style

Felipe Antonio Fernandes Antunes, Kalavathy Rajan, ANGELE Djioleu, Thiago Moura Rocha, Larissa Pereira Brumano, Yasmin Cristhine De Souza Melo, Júlio César dos Santos, Carlos A. Rosa, Danielle Julie Carrier, Silvio Silvério da Silva. Sustainable Second-Generation Ethanol Production from Switchgrass Biomass via Co-fermentation of Pentoses and Hexoses Using Novel Wild Yeasts. BioEnergy Research. 2021; ():1-12.

Chicago/Turabian Style

Felipe Antonio Fernandes Antunes; Kalavathy Rajan; ANGELE Djioleu; Thiago Moura Rocha; Larissa Pereira Brumano; Yasmin Cristhine De Souza Melo; Júlio César dos Santos; Carlos A. Rosa; Danielle Julie Carrier; Silvio Silvério da Silva. 2021. "Sustainable Second-Generation Ethanol Production from Switchgrass Biomass via Co-fermentation of Pentoses and Hexoses Using Novel Wild Yeasts." BioEnergy Research , no. : 1-12.

Journal article
Published: 07 July 2021 in Fermentation
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Lasiodiplodan, a (1→6)-β-d-glucan, is an exopolysaccharide with high commercial value and many applications in food, pharmaceuticals, and cosmetics. Current industrial production of β-glucans from crops is mostly by chemical routes generating hazardous and toxic waste. Therefore, alternative sustainable and eco-friendly pathways are highly desirable. Here, we have studied the lasiodiplodan production from sugarcane bagasse (SCB), a major lignocellulosic agricultural residue, by Lasiodiplodia theobromae CCT 3966. Lasiodiplodan accumulated on SCB hydrolysate (carbon source) supplemented with soybean bran or rice bran (nitrogen source) was 16.2 [6.8 × 103 Da] and 22.0 [7.6 × 103 Da] g/L, respectively. Lasiodiplodan showed high purity, low solubility, pseudoplastic behavior and was composed of glucose units. Moreover, the exopolysaccharides were substantially amorphous with moderate thermal stability and similar degradation temperatures. To our knowledge, this is the first report on the highest production of SCB-based lasiodiplodan to date. L. theobromae, as a microbial cell factory, demonstrated the commercial potential for the sustainable production of lasiodiplodan from renewable biomass feedstock.

ACS Style

Jesús Ascencio; Rafael Philippini; Fabricio Gomes; Félix Pereira; Silvio da Silva; Vinod Kumar; Anuj Chandel. Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization. Fermentation 2021, 7, 108 .

AMA Style

Jesús Ascencio, Rafael Philippini, Fabricio Gomes, Félix Pereira, Silvio da Silva, Vinod Kumar, Anuj Chandel. Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization. Fermentation. 2021; 7 (3):108.

Chicago/Turabian Style

Jesús Ascencio; Rafael Philippini; Fabricio Gomes; Félix Pereira; Silvio da Silva; Vinod Kumar; Anuj Chandel. 2021. "Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization." Fermentation 7, no. 3: 108.

Chapter
Published: 18 February 2021 in Green Organic Reactions
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The human population on the planet earth is increasing with a faster pace which is expected to cross the figure of 8 billion very soon. Therefore, feeding such huge population is a major challenge; moreover, various other problems such as improper infrastructure and transport lead to food wastage. In addition, microbial contamination in food results to inferior food quality. Therefore, significant increase in food crop production along with management of food wastage is of great importance to overcome the food crisis. In this context, considering the broad spectrum applications of nanotechnology and nanomaterials in various fields including food industries, it is believed that nanotechnological solutions would be revolutionary as far as various problems of food industries are concerned. Considering these facts, in the present chapter, we have summarized synthesis of different nanomaterials using different microorganisms, and recent developments in the field of nanotechnology and its role in food industries particularly in food processing and food packaging are also discussed.

ACS Style

A. P. Ingle; R. Philippini; S. E. Martiniano; F. A. F. Antunes; T. M. Rocha; S. S. da Silva. Application of Microbial-Synthesized Nanoparticles in Food Industries. Green Organic Reactions 2021, 399 -424.

AMA Style

A. P. Ingle, R. Philippini, S. E. Martiniano, F. A. F. Antunes, T. M. Rocha, S. S. da Silva. Application of Microbial-Synthesized Nanoparticles in Food Industries. Green Organic Reactions. 2021; ():399-424.

Chicago/Turabian Style

A. P. Ingle; R. Philippini; S. E. Martiniano; F. A. F. Antunes; T. M. Rocha; S. S. da Silva. 2021. "Application of Microbial-Synthesized Nanoparticles in Food Industries." Green Organic Reactions , no. : 399-424.

Journal article
Published: 13 December 2020 in Metabolites
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The production of biomolecules using agro-industrial by-products as feedstock is a growing trend worldwide. Selenium (Se) is a trace element essential for health, and the Se-enrichment of yeast biomass can enhance its benefits. This study investigated the feasibility of the production of Saccharomyces cerevisiae Se-enriched biomass using a medium composed of corn bran and soybean bran acid hydrolysates as carbon and nitrogen sources in a stirred-tank reactor. After hydrolysis, hydrolysates presented complex composition and high concentrations of sugars, proteins, and minerals. The use of a stirred-tank bioreactor leads to the production of 9 g/L S. cerevisiae biomass enriched with 236.93 μg/g Se, and 99% cell viability. Likewise, the combination of sugarcane molasses and soybean bran hydrolysate was effective for cell growth of a probiotic strain of S. cerevisiae with a 24.08% β-glucan content. The results demonstrated that starchy acid hydrolysates are low-cost and efficient substrates for the production of yeast biomass and derivate products and may contribute to further studies for a sustainable development of biorefinery technologies.

ACS Style

Sabrina Evelin Martiniano; Letícia Alves Fernandes; Edith Mier Alba; Rafael Rodrigues Philippini; Stephanie Caroline Tavares Tabuchi; Marek Kieliszek; Júlio César Dos Santos; Silvio Silvério Da Silva. A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor. Metabolites 2020, 10, 508 .

AMA Style

Sabrina Evelin Martiniano, Letícia Alves Fernandes, Edith Mier Alba, Rafael Rodrigues Philippini, Stephanie Caroline Tavares Tabuchi, Marek Kieliszek, Júlio César Dos Santos, Silvio Silvério Da Silva. A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor. Metabolites. 2020; 10 (12):508.

Chicago/Turabian Style

Sabrina Evelin Martiniano; Letícia Alves Fernandes; Edith Mier Alba; Rafael Rodrigues Philippini; Stephanie Caroline Tavares Tabuchi; Marek Kieliszek; Júlio César Dos Santos; Silvio Silvério Da Silva. 2020. "A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor." Metabolites 10, no. 12: 508.

Review article
Published: 29 July 2020 in Frontiers in Energy Research
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The integrated approach in biorefinery mainly involves the utilization of various agroindustrial byproducts such as raw materials for the production of several biobased products like biofuels, bioenergy, and other high-value chemicals. Biofuels are the backbone of biorefineries, however, production of value-added biomolecules such as biopigments, biopolymers, biosurfactants, and nutritional yeast has been attracting great attention. The production of these biomolecules using traditional approaches has been extensively studied in the last few years owing to their promising application in different industries such as chemical, food/feed, and pharmaceuticals for the development of novel products for mankind. Moreover, the production of such biomolecules using lignocellulosic, starchy, and some other agroindustrial byproducts is still not fully explored. Hence, there is a huge scope in the development of sustainable biorefining approaches to make the technology cost-effective. The lignocellulosic biomasses usually used in biorefineries are mainly composed of cellulose, hemicellulose, and lignin, whereas starchy materials, besides starch, usually contain, protein, lipids, and some micronutrients. The processing of these biomasses through successive steps like pretreatments, enzymatic hydrolysis, and fermentation is essentially required to obtained final biobased products. Considering certain bottlenecks of above-mentioned conventional biorefineries approaches, new technologies have been proposed for the improved pretreatment of biomass and efficient enzymatic hydrolysis in order to minimize the concentration of toxic inhibitors in resulting hydrolysate. In this review, we highlighted the different agroindustrial byproducts and their applications for the production of valuable biorefinery products.

ACS Style

Rafael Rodrigues Philippini; Sabrina Evelin Martiniano; Avinash P. Ingle; Paulo Ricardo Franco Marcelino; Gilda Mariano Silva; Fernanda Gonçalves Barbosa; Júlio Santos; Silvio Silvério Da Silva. Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries. Frontiers in Energy Research 2020, 8, 1 .

AMA Style

Rafael Rodrigues Philippini, Sabrina Evelin Martiniano, Avinash P. Ingle, Paulo Ricardo Franco Marcelino, Gilda Mariano Silva, Fernanda Gonçalves Barbosa, Júlio Santos, Silvio Silvério Da Silva. Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries. Frontiers in Energy Research. 2020; 8 ():1.

Chicago/Turabian Style

Rafael Rodrigues Philippini; Sabrina Evelin Martiniano; Avinash P. Ingle; Paulo Ricardo Franco Marcelino; Gilda Mariano Silva; Fernanda Gonçalves Barbosa; Júlio Santos; Silvio Silvério Da Silva. 2020. "Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries." Frontiers in Energy Research 8, no. : 1.

Original article
Published: 03 June 2020 in Biomass Conversion and Biorefinery
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Sugarcane bagasse (SCB) was pretreated with 1-butyl-3-methylimidazolium chloride (BmimCl) at different conditions of temperature (80 °C to 150 °C) and solid loading (4 to 10%) at two times (20 and 60 min). The pretreatment conditions were optimized using a central composite rotatable design (CCRD) and desirability function having in mind the principles of green engineering. The pretreatments resulted in modifications of morphological and structural characteristics of biomass, also resulting in partial hemicellulose reduction. The founded optimal condition of pretreatment under the criterion of maximized sugar yield after enzymatic hydrolysis and minimized total sugar loss in pretreatment was 140 °C and 6% w/w of solid loading with 20 min of process. Also, a kinetic model was obtained verifying its validity with experimental values. It showed a lower activation energy of cellulose modification and hemicellulose conversion in comparison with the literature. One of the major findings was the strong correlation between glucose conversion and the degree of polymerization.

ACS Style

Leyanis Mesa; Victor Soares Valerio; Marcus Forte; Júlio C. Santos; Erenio González; Silvio S. Da Silva. Optimization of BmimCl pretreatment of sugarcane bagasse through combining multiple responses to increase sugar production. An approach of the kinetic model. Biomass Conversion and Biorefinery 2020, 1 -17.

AMA Style

Leyanis Mesa, Victor Soares Valerio, Marcus Forte, Júlio C. Santos, Erenio González, Silvio S. Da Silva. Optimization of BmimCl pretreatment of sugarcane bagasse through combining multiple responses to increase sugar production. An approach of the kinetic model. Biomass Conversion and Biorefinery. 2020; ():1-17.

Chicago/Turabian Style

Leyanis Mesa; Victor Soares Valerio; Marcus Forte; Júlio C. Santos; Erenio González; Silvio S. Da Silva. 2020. "Optimization of BmimCl pretreatment of sugarcane bagasse through combining multiple responses to increase sugar production. An approach of the kinetic model." Biomass Conversion and Biorefinery , no. : 1-17.

Original research
Published: 02 June 2020 in Cellulose
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The ubiquitous nature of lignocellulosic biomass on planet earth and its economic viability attracted a great deal of attention from researchers and becomes foremost feedstock for biofuel production particularly bioethanol. However, due to complexity in structure, its pretreatment is essentially required prior to actual use. In the present study, a promising approach has been proposed through the development of acid-functionalized magnetic nanocatalysts. Two different acid-functionalized magnetic nanocatalysts i.e. alkylsulfonic acid functionalized magnetic nanoparticles (Fe3O4-MNPs-Si-AS) and butylcarboxylic acid functionalized magnetic nanoparticles (Fe3O4-MNPs-Si-BCOOH) were developed and their efficacy was studied in the pretreatment of sugarcane straw at varying concentrations (100, 200, 300, 400, 500 mg/g of straw). The enhanced concentration dependent production of sugar (xylose) was reported in case of both the nanocatalysts. The maximum 17.06 g/L for Fe3O4-MNPs-Si-AS and 15.40 g/L for Fe3O4-MNPs-Si-BCOOH sugar was reported at 500 mg which is comparatively higher than normal acid (H2SO4) (14.63 g/L) and non-treated (0.24 g/L) sugarcane straw. Further, both the nanocatalysts were recovered by applying an external magnetic field and reused for the next two subsequent cycles of pretreatment. It was observed that with every reuse of nanocatalysts the concentration of sugar production was reduced. Moreover, generation of very less amount of toxic inhibitors was reported in the hemicellulosic hydrolyzate obtained in the present study. Considering these facts, it is believed that such nanocatalysts can be used as an effective, eco-friendly and economically viable alternative to the conventional pretreatment agents like mineral acids.

ACS Style

Avinash P. Ingle; Rafael Philippini; Yasmin Cristhine De Souza Melo; Silvio Silvério Da Silva. Acid-functionalized magnetic nanocatalysts mediated pretreatment of sugarcane straw: an eco-friendly and cost-effective approach. Cellulose 2020, 27, 7067 -7078.

AMA Style

Avinash P. Ingle, Rafael Philippini, Yasmin Cristhine De Souza Melo, Silvio Silvério Da Silva. Acid-functionalized magnetic nanocatalysts mediated pretreatment of sugarcane straw: an eco-friendly and cost-effective approach. Cellulose. 2020; 27 (12):7067-7078.

Chicago/Turabian Style

Avinash P. Ingle; Rafael Philippini; Yasmin Cristhine De Souza Melo; Silvio Silvério Da Silva. 2020. "Acid-functionalized magnetic nanocatalysts mediated pretreatment of sugarcane straw: an eco-friendly and cost-effective approach." Cellulose 27, no. 12: 7067-7078.

Review
Published: 07 February 2020 in Symmetry
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The excessive consumption of petroleum resources leads to global warming, fast depletion of petroleum reserves, as well as price instability of gasoline. Thus, there is a strong need for alternative renewable fuels to replace petroleum-derived fuels. The striking features of an alternative fuel include the low carbon footprints, renewability and affordability at manageable prices. Biodiesel, made from waste oils, animal fats, vegetal oils, is a totally renewable and non-toxic liquid fuel which has gained significant attraction in the world. Due to technological advancements in catalytic chemistry, biodiesel can be produced from a variety of feedstock employing a variety of catalysts and recovery technologies. Recently, several ground-breaking advancements have been made in nano-catalyst technology which showed the symmetrical correlation with cost competitive biodiesel production. Nanocatalysts have unique properties such as their selective reactivity, high activation energy and controlled rate of reaction, easy recovery and recyclability. Here, we present an overview of various feedstock used for biodiesel production, their composition and characteristics. The major focus of this review is to appraise the characterization of nanocatalysts, their effect on biodiesel production and methodologies of biodiesel production.

ACS Style

Avinash P. Ingle; Anuj K. Chandel; Rafael Philippini; Sabrina Evelin Martiniano; Silvio Silvério Da Silva. Advances in Nanocatalysts Mediated Biodiesel Production: A Critical Appraisal. Symmetry 2020, 12, 256 .

AMA Style

Avinash P. Ingle, Anuj K. Chandel, Rafael Philippini, Sabrina Evelin Martiniano, Silvio Silvério Da Silva. Advances in Nanocatalysts Mediated Biodiesel Production: A Critical Appraisal. Symmetry. 2020; 12 (2):256.

Chicago/Turabian Style

Avinash P. Ingle; Anuj K. Chandel; Rafael Philippini; Sabrina Evelin Martiniano; Silvio Silvério Da Silva. 2020. "Advances in Nanocatalysts Mediated Biodiesel Production: A Critical Appraisal." Symmetry 12, no. 2: 256.

Modeling and analysis
Published: 20 November 2019 in Biofuels, Bioproducts and Biorefining
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Xylitol was produced by Scheffersomyces amazonensis UFMG‐HMD‐26.3 in hemicellulosic hydrolysate obtained from a mixture of sugarcane bagasse and straw (1:1) in batch fermentation, and was recovered using supercritical fluid. The xylitol produced was 20.11 g L−1 and the extraction approach reached a xylitol recovery of 40.51% at a high purity level of 99.59%. An analysis of energy consumption for the xylitol production process showed that xylitol production from sugarcane biomass hemicellulosic hydrolysate is feasible, highlighting the potential use of this biomass in sustainable xylitol production and supercritical fluids as a robust proposal for xylitol separation. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

ACS Style

Débora D.V. Silva; Kelly J. Dussán; Angela Idarraga; Luana Grangeiro; Silvio S. Silva; Carlos A. Cardona; Julián Quintero; Maria G.A. Felipe. Production and purification of xylitol by Scheffersomyces amazonenses via sugarcane hemicellulosic hydrolysate. Biofuels, Bioproducts and Biorefining 2019, 14, 344 -356.

AMA Style

Débora D.V. Silva, Kelly J. Dussán, Angela Idarraga, Luana Grangeiro, Silvio S. Silva, Carlos A. Cardona, Julián Quintero, Maria G.A. Felipe. Production and purification of xylitol by Scheffersomyces amazonenses via sugarcane hemicellulosic hydrolysate. Biofuels, Bioproducts and Biorefining. 2019; 14 (2):344-356.

Chicago/Turabian Style

Débora D.V. Silva; Kelly J. Dussán; Angela Idarraga; Luana Grangeiro; Silvio S. Silva; Carlos A. Cardona; Julián Quintero; Maria G.A. Felipe. 2019. "Production and purification of xylitol by Scheffersomyces amazonenses via sugarcane hemicellulosic hydrolysate." Biofuels, Bioproducts and Biorefining 14, no. 2: 344-356.

Journal article
Published: 01 August 2018 in Renewable Energy
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ACS Style

F.A.F. Antunes; A.K. Chandel; Larissa Brumano; R. Terán Hilares; G.F.D. Peres; L.E.S. Ayabe; V.S. Sorato; Júlio Santos; Silvio Silvério da Silva. A novel process intensification strategy for second-generation ethanol production from sugarcane bagasse in fluidized bed reactor. Renewable Energy 2018, 124, 189 -196.

AMA Style

F.A.F. Antunes, A.K. Chandel, Larissa Brumano, R. Terán Hilares, G.F.D. Peres, L.E.S. Ayabe, V.S. Sorato, Júlio Santos, Silvio Silvério da Silva. A novel process intensification strategy for second-generation ethanol production from sugarcane bagasse in fluidized bed reactor. Renewable Energy. 2018; 124 ():189-196.

Chicago/Turabian Style

F.A.F. Antunes; A.K. Chandel; Larissa Brumano; R. Terán Hilares; G.F.D. Peres; L.E.S. Ayabe; V.S. Sorato; Júlio Santos; Silvio Silvério da Silva. 2018. "A novel process intensification strategy for second-generation ethanol production from sugarcane bagasse in fluidized bed reactor." Renewable Energy 124, no. : 189-196.

Chapter
Published: 01 January 2018 in Nanomaterials: Ecotoxicity, Safety, and Public Perception
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Recent advances in the field of nanoscience and nanotechnology enabled the development of precise processes that are used across the most diverse science fields to control individual atoms and molecules. Inorganic nanomaterials, specially zinc and silver nanoparticles (Zn-NPs, Ag-NPs), have been attracting attention in the last decades due to their versatility, with applications as active componentes in eletronics, pharmaceuticals, cosmetic and agricultural products. However, safe nanotechnology unfortunatelly did not progress at the same speed, and hazardous nanomaterials are still disposed indiscriminately, crompromising the environment. Herein, the most recent and relevant contributions related to Zn-NPs and Ag-NPs, with regard to their chemical properties, utilization, disposal, interaction with biological systems and environmental impacts, are presented and discussed.

ACS Style

Paulo Ricardo Franco Marcelino; Mariete Barbosa Moreira; Talita Martins Lacerda; Silvio Silvério Da Silva. Zinc and Silver Nanoparticles: Properties, Applications and Impact to the Aquatic Environment. Nanomaterials: Ecotoxicity, Safety, and Public Perception 2018, 167 -190.

AMA Style

Paulo Ricardo Franco Marcelino, Mariete Barbosa Moreira, Talita Martins Lacerda, Silvio Silvério Da Silva. Zinc and Silver Nanoparticles: Properties, Applications and Impact to the Aquatic Environment. Nanomaterials: Ecotoxicity, Safety, and Public Perception. 2018; ():167-190.

Chicago/Turabian Style

Paulo Ricardo Franco Marcelino; Mariete Barbosa Moreira; Talita Martins Lacerda; Silvio Silvério Da Silva. 2018. "Zinc and Silver Nanoparticles: Properties, Applications and Impact to the Aquatic Environment." Nanomaterials: Ecotoxicity, Safety, and Public Perception , no. : 167-190.

Book chapter
Published: 01 January 2018 in Advances in Sugarcane Biorefinery
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ACS Style

João R.M. Almeida; Felipe A.F. Antunes; Valdeir Arantes; Richa Arora; Mirian Rumenos Piedade Bacchi; Jessica C. Bergmann; Fernando C. Bertolani; Prasanna Borse; Anuj K. Chandel; Junio Cota; Haroldo J.T. Da Silva; Silvio S. Da Silva; Awana Da Silva Lima; Natália De Campos Trombeta; Lilian Maluf De Lima; Carlaile F. De Oliveira Nogueira; Flávia Cristina De Paula E Silva; Marina O.S. Dias; Jorge L. Donzelli; Julio C. Dos Santos; Simo Ellilä; Gislene Ferreira; Bruno A. França; Rajiv Ganguly; Vijay Kumar Garlapati; Aline L. Gonçalves; André T.V. Hermann; Sachin Kumar; Danilo R. Lima; Adriano P. Mariano; Mahdi Mazuchi; Evandro J. Mulinari; Bianca Oliva; Barbara Pereira; Marcelo A. Pierossi; Luciano Rodrigues; Lunalva P. Sallet; Fernando Segato; Nilesh K. Sharma; Amol Sheth; Marcos H.L. Silveira; Ruly Terán-Hilares; Debora Trichez; Bruno A. Vanelli; Josman Velasco; Carlos E.F. Vian. List of Contributors. Advances in Sugarcane Biorefinery 2018, 1 .

AMA Style

João R.M. Almeida, Felipe A.F. Antunes, Valdeir Arantes, Richa Arora, Mirian Rumenos Piedade Bacchi, Jessica C. Bergmann, Fernando C. Bertolani, Prasanna Borse, Anuj K. Chandel, Junio Cota, Haroldo J.T. Da Silva, Silvio S. Da Silva, Awana Da Silva Lima, Natália De Campos Trombeta, Lilian Maluf De Lima, Carlaile F. De Oliveira Nogueira, Flávia Cristina De Paula E Silva, Marina O.S. Dias, Jorge L. Donzelli, Julio C. Dos Santos, Simo Ellilä, Gislene Ferreira, Bruno A. França, Rajiv Ganguly, Vijay Kumar Garlapati, Aline L. Gonçalves, André T.V. Hermann, Sachin Kumar, Danilo R. Lima, Adriano P. Mariano, Mahdi Mazuchi, Evandro J. Mulinari, Bianca Oliva, Barbara Pereira, Marcelo A. Pierossi, Luciano Rodrigues, Lunalva P. Sallet, Fernando Segato, Nilesh K. Sharma, Amol Sheth, Marcos H.L. Silveira, Ruly Terán-Hilares, Debora Trichez, Bruno A. Vanelli, Josman Velasco, Carlos E.F. Vian. List of Contributors. Advances in Sugarcane Biorefinery. 2018; ():1.

Chicago/Turabian Style

João R.M. Almeida; Felipe A.F. Antunes; Valdeir Arantes; Richa Arora; Mirian Rumenos Piedade Bacchi; Jessica C. Bergmann; Fernando C. Bertolani; Prasanna Borse; Anuj K. Chandel; Junio Cota; Haroldo J.T. Da Silva; Silvio S. Da Silva; Awana Da Silva Lima; Natália De Campos Trombeta; Lilian Maluf De Lima; Carlaile F. De Oliveira Nogueira; Flávia Cristina De Paula E Silva; Marina O.S. Dias; Jorge L. Donzelli; Julio C. Dos Santos; Simo Ellilä; Gislene Ferreira; Bruno A. França; Rajiv Ganguly; Vijay Kumar Garlapati; Aline L. Gonçalves; André T.V. Hermann; Sachin Kumar; Danilo R. Lima; Adriano P. Mariano; Mahdi Mazuchi; Evandro J. Mulinari; Bianca Oliva; Barbara Pereira; Marcelo A. Pierossi; Luciano Rodrigues; Lunalva P. Sallet; Fernando Segato; Nilesh K. Sharma; Amol Sheth; Marcos H.L. Silveira; Ruly Terán-Hilares; Debora Trichez; Bruno A. Vanelli; Josman Velasco; Carlos E.F. Vian. 2018. "List of Contributors." Advances in Sugarcane Biorefinery , no. : 1.

Original paper
Published: 29 December 2017 in Waste and Biomass Valorization
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Cell wall composition in lignocellulosic biomass varies depending on genetic origin, growth conditions, weather and soil conditions. Here, we have evaluated the chemical characterization, morphology and enzymatic hydrolysis efficiencies (after sequential dilute acid-base pretreatment) of sugarcane bagasse from five different hybrid varieties of sugarcane. On average, bagasse samples showed cellulose, hemicellulose, lignin and ash compositions of 40.84, 24.07, 33.71 and 0.68%, respectively. Sequential acid-base pretreatment removed approximately 77 and 58% hemicellulose and lignin, respectively, leaving pulp samples rich in cellulose (up to 80%), which exhibited a maximum saccharification yields of 55.39% after enzymatic hydrolysis. We found average contents of cellulose (54.17 and 77.48%), hemicellulose (5.64 and 6.07%), lignin (37.28 and 15.40%) and ash (0.54 and 0.32%) in cellulignin and cellulosic pulp, respectively. Results showed that the genetic variability of sugarcane had no influence on the chemical composition and sugar recovery after saccharification of bagasse samples. Therefore, sugarcane bagasse from these new sugarcane varieties samples may be used for second generation sugars production. Cellulosic sugars may serve as primary building block for renewable fuels and chemicals production at commercial scale under biorefinery concept.

ACS Style

Rafael Philippini; Sabrina E. Martiniano; Anuj K. Chandel; Walter De Carvalho; Silvio S. Da Silva. Pretreatment of Sugarcane Bagasse from Cane Hybrids: Effects on Chemical Composition and 2G Sugars Recovery. Waste and Biomass Valorization 2017, 10, 1561 -1570.

AMA Style

Rafael Philippini, Sabrina E. Martiniano, Anuj K. Chandel, Walter De Carvalho, Silvio S. Da Silva. Pretreatment of Sugarcane Bagasse from Cane Hybrids: Effects on Chemical Composition and 2G Sugars Recovery. Waste and Biomass Valorization. 2017; 10 (6):1561-1570.

Chicago/Turabian Style

Rafael Philippini; Sabrina E. Martiniano; Anuj K. Chandel; Walter De Carvalho; Silvio S. Da Silva. 2017. "Pretreatment of Sugarcane Bagasse from Cane Hybrids: Effects on Chemical Composition and 2G Sugars Recovery." Waste and Biomass Valorization 10, no. 6: 1561-1570.

Research article
Published: 10 November 2017 in PLOS ONE
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Biosurfactants are microbial metabolites with possible applications in various industrial sectors that are considered ecofriendly molecules. In recent years, some studies identified these compounds as alternatives for the elimination of vectors of tropical diseases, such as Aedes aegypti. The major bottlenecks of biosurfactant industrial production have been the use of conventional raw materials that increase production costs as well as opportunistic or pathogenic bacteria, which restrict the application of these biomolecules. The present study shows the potential of hemicellulosic sugarcane bagasse hydrolysate as a raw material for the production of a crystalline glycolipidic BS by Scheffersomyces stipitis NRRL Y-7124, which resulted in an emulsifying index (EI24) of 70 ± 3.4% and a superficial tension of 52 ± 2.9 mN.m-1. Additionally, a possible new application of these compounds as biolarvicides, mainly against A. aegypti, was evaluated. At a concentration of 800 mg.L-1, the produced biosurfactant caused destruction to the larval exoskeletons 12 h after application and presented an letal concentration (LC50) of 660 mg.L-1. Thus, a new alternative for biosurfactant production using vegetal biomass as raw material within the concept of biorefineries was proposed, and the potential of the crystalline glycolipidic biosurfactant in larvicidal formulations against neglected tropical disease vectors was demonstrated.

ACS Style

Paulo Ricardo Franco Marcelino; Vinícius Luiz Da Silva; Rafael Philippini; Cláudio José Von Zuben; Jonas Contiero; Júlio Santos; Silvio Silvério da Silva. Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases. PLOS ONE 2017, 12, e0187125 .

AMA Style

Paulo Ricardo Franco Marcelino, Vinícius Luiz Da Silva, Rafael Philippini, Cláudio José Von Zuben, Jonas Contiero, Júlio Santos, Silvio Silvério da Silva. Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases. PLOS ONE. 2017; 12 (11):e0187125.

Chicago/Turabian Style

Paulo Ricardo Franco Marcelino; Vinícius Luiz Da Silva; Rafael Philippini; Cláudio José Von Zuben; Jonas Contiero; Júlio Santos; Silvio Silvério da Silva. 2017. "Biosurfactants produced by Scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of Aedes aegypti, a vector of neglected tropical diseases." PLOS ONE 12, no. 11: e0187125.

Journal article
Published: 01 November 2017 in Bioresource Technology
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Surfactants are amphiphilic molecules with large industrial applications produced currently by chemical routes mainly derived from oil industry. However, biotechnological process, aimed to develop new sustainable process configurations by using favorable microorganisms, already requires investigations in more details. Thus, we present a novel approach for biosurfactant production using the promising yeast Aureobasidium pullulans LB 83, in stirred tank reactor. A central composite face-centered design was carried out to evaluate the effect of the aeration rate (0.1-1.1min(-1)) and sucrose concentration (20-80g.L(-1)) in the biosurfactant maximum tensoactivity and productivity. Statistical analysis showed that the use of variables at high levels enhanced tensoactivity, showing 8.05cm in the oil spread test and productivity of 0.0838cm.h(-1). Also, unprecedented investigation of aeration rate and sucrose concentration relevance in biosurfactant production by A. pullulans in stirred tank reactor was detailed, demonstrating the importance to establish adequate conditions in bioreactors, aimed to scale-up process.

ACS Style

Larissa Pereira Brumano; Felipe Antonio Fernandes Antunes; Sara Galeno Souto; Júlio Santos; Joachim Venus; Roland Schneider; Silvio Silvério da Silva. Biosurfactant production by Aureobasidium pullulans in stirred tank bioreactor: New approach to understand the influence of important variables in the process. Bioresource Technology 2017, 243, 264 -272.

AMA Style

Larissa Pereira Brumano, Felipe Antonio Fernandes Antunes, Sara Galeno Souto, Júlio Santos, Joachim Venus, Roland Schneider, Silvio Silvério da Silva. Biosurfactant production by Aureobasidium pullulans in stirred tank bioreactor: New approach to understand the influence of important variables in the process. Bioresource Technology. 2017; 243 ():264-272.

Chicago/Turabian Style

Larissa Pereira Brumano; Felipe Antonio Fernandes Antunes; Sara Galeno Souto; Júlio Santos; Joachim Venus; Roland Schneider; Silvio Silvério da Silva. 2017. "Biosurfactant production by Aureobasidium pullulans in stirred tank bioreactor: New approach to understand the influence of important variables in the process." Bioresource Technology 243, no. : 264-272.

Journal article
Published: 01 November 2017 in Bioresource Technology
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In this study, sugarcane bagasse (SCB) pretreated with alkali assisted hydrodynamic cavitation (HC) was investigated for simultaneous saccharification and fermentation (SSF) process for bioethanol production in interconnected column reactors using immobilized Scheffersomyces stipitis NRRL-Y7124. Initially, HC was employed for the evaluation of the reagent used in alkaline pretreatment. Alkalis (NaOH, KOH, NaCO, Ca(OH)) and NaOH recycled black liquor (successive batches) were used and their pretreatment effectiveness was assessed considering the solid composition and its enzymatic digestibility. In SSF process using NaOH-HC pretreatment SCB, 62.33% of total carbohydrate fractions were hydrolyzed and 17.26g/L of ethanol production (0.48g of ethanol/g of glucose and xylose consumed) was achieved. This proposed scheme of HC-assisted NaOH pretreatment together with our interconnected column reactors showed to be an interesting new approach for biorefineries.

ACS Style

Ruly Terán Hilares; João Vitor Ienny; Paulo Franco Marcelino; Muhammad Ajaz Ahmed; Felipe A.F. Antunes; Silvio Silvério da Silva; Júlio Santos. Ethanol production in a simultaneous saccharification and fermentation process with interconnected reactors employing hydrodynamic cavitation-pretreated sugarcane bagasse as raw material. Bioresource Technology 2017, 243, 652 -659.

AMA Style

Ruly Terán Hilares, João Vitor Ienny, Paulo Franco Marcelino, Muhammad Ajaz Ahmed, Felipe A.F. Antunes, Silvio Silvério da Silva, Júlio Santos. Ethanol production in a simultaneous saccharification and fermentation process with interconnected reactors employing hydrodynamic cavitation-pretreated sugarcane bagasse as raw material. Bioresource Technology. 2017; 243 ():652-659.

Chicago/Turabian Style

Ruly Terán Hilares; João Vitor Ienny; Paulo Franco Marcelino; Muhammad Ajaz Ahmed; Felipe A.F. Antunes; Silvio Silvério da Silva; Júlio Santos. 2017. "Ethanol production in a simultaneous saccharification and fermentation process with interconnected reactors employing hydrodynamic cavitation-pretreated sugarcane bagasse as raw material." Bioresource Technology 243, no. : 652-659.

Original paper
Published: 09 October 2017 in Cellulose
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Production of bioethanol from various lignocellulosic biomass through enzymatic hydrolysis is considered as a promising approach to fulfill the global energy demand. In addition to overcoming the worldwide energy crisis, it also plays an important role in the management of lignocellulosic waste. We have synthesized iron oxide nanoparticles (magnetic nanoparticles—MNPs) using cell filtrate of fungus, Alternaria alternata. The synthesis of MNPs was initially confirmed by the visual observation followed by characterization using different analytical techniques. The NTA and TEM analysis showed the average size of 47 and 55 nm respectively. XRD analysis confirmed the FCC structure of nanoparticles and zeta potential for MNPs was − 7.06 mV, which indicated the stability of nanoparticles. Further, comparative evaluation of enzymatic hydrolysis of lignocellulosic biomass (sugarcane bagasse) using free and immobilized cellulase on MNPs at different temperatures was studied. The results obtained demonstrated that, in first cycle of hydrolysis, free enzyme was more efficient which showed about 78% conversion of cellulose to glucose at 40 °C after 24 h, whereas in case of immobilized enzyme it was found to be 72%. Moreover, immobilized cellulase was recovered by applying magnetic field and reused up to third cycle of hydrolysis. In second and third cycle, rate of conversion of cellulose to glucose was found to be 68 and 52% respectively. These findings suggest that immobilization of cellulase on MNPs facilitate the easy recovery and their reuse for more than one cycle of hydrolysis, thereby making the process economically viable. Further, optimization and modification of certain conditions will be helpful to increase the efficiency of immobilized enzyme.

ACS Style

Avinash P. Ingle; Jyoti Rathod; Raksha Pandit; Silvio Silvério da Silva; Mahendra Rai. Comparative evaluation of free and immobilized cellulase for enzymatic hydrolysis of lignocellulosic biomass for sustainable bioethanol production. Cellulose 2017, 24, 5529 -5540.

AMA Style

Avinash P. Ingle, Jyoti Rathod, Raksha Pandit, Silvio Silvério da Silva, Mahendra Rai. Comparative evaluation of free and immobilized cellulase for enzymatic hydrolysis of lignocellulosic biomass for sustainable bioethanol production. Cellulose. 2017; 24 (12):5529-5540.

Chicago/Turabian Style

Avinash P. Ingle; Jyoti Rathod; Raksha Pandit; Silvio Silvério da Silva; Mahendra Rai. 2017. "Comparative evaluation of free and immobilized cellulase for enzymatic hydrolysis of lignocellulosic biomass for sustainable bioethanol production." Cellulose 24, no. 12: 5529-5540.

Journal article
Published: 02 October 2017 in Orbital: The Electronic Journal of Chemistry
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The ability of twenty-three yeast strains isolated from decayed wood and the fungus garden of attine ants to produce hemi- and cellulolytic hydrolases in a chemically defined medium was screened. Xylanase, β-glucosidase and cellulase activities were found in yeasts strains in the genera Cryptococcus, Trichosporon, Debaryomyces and Pichia. Among the isolated microorganisms, two strains of Trichosporon laibachii MG270406-1A14 strain showed higher cellulase titers. Several biochemical and physiological parameters were investigated for optimum cellulase production under submerged fermentation. Enzyme induction was also examined using different carbon and nitrogen sources. Cellobiose and ammonium sulphate were found best carbon (C) and nitrogen (N) sources to enhance carboxymethylcellulase production. A 22- factorial design with center points was developed to optimize enzyme production, and data analysed by the response surface method. Maximal enzyme titres (0.3 U mL-1) occurred at initial pH 6.0, C/N ratio 9.0 and 5 days. DOI: http://dx.doi.org/10.17807/orbital.v9i4.1024

ACS Style

Ellen Cristine Giese; Kelly J. Dussán; Maurício Pierozzi; Anuj Kumar Chandel; Fernando Carlos Pagnocca; Sílvio Silvério Da Silva. Cellulase Production by Trichosporon laibachii. Orbital: The Electronic Journal of Chemistry 2017, 9, 1 .

AMA Style

Ellen Cristine Giese, Kelly J. Dussán, Maurício Pierozzi, Anuj Kumar Chandel, Fernando Carlos Pagnocca, Sílvio Silvério Da Silva. Cellulase Production by Trichosporon laibachii. Orbital: The Electronic Journal of Chemistry. 2017; 9 (4):1.

Chicago/Turabian Style

Ellen Cristine Giese; Kelly J. Dussán; Maurício Pierozzi; Anuj Kumar Chandel; Fernando Carlos Pagnocca; Sílvio Silvério Da Silva. 2017. "Cellulase Production by Trichosporon laibachii." Orbital: The Electronic Journal of Chemistry 9, no. 4: 1.

Journal article
Published: 11 September 2017 in Journal of Applied Microbiology
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This study aimed to evaluate new d-xylose-fermenting yeasts from Brazilian ecosystems for the production of second-generation ethanol. d-xylose-fermenting yeasts isolated from rotting wood and wood-boring insects were identified as the species Scheffersomyces parashehatae, Scheffersomyces illinoinensis, Spathaspora arborariae and Wickerhamomyces rabaulensis. Among the yeasts tested, those of Sc. parashehatae exhibited the highest ethanol production when cultivated on complex medium (Yp/set = 0·437 g g−1). Sheffersomyces illinoinensis and Sp. arborariae showed similar ethanol production in this assay (Yp/set up to 0·295 g g−1). In contrast, in sugarcane bagasse hemicellulosic hydrolysate, Sc. parashehatae and Sc. illinoinensis exhibited similar ethanol production (Yp/set up to 0·254 g g−1), whereas Sp. arborariae showed the lowest results (peak Yp/set = 0·160 g g−1). Wickerhamomyces rabaulensis exhibited a remarkable xylitol production (Yp/sxyl = 0·681 g g−1), but producing low levels of ethanol (Yp/set = 0·042 g g−1). The novel d-xylose-fermenting yeasts showed promising metabolic characteristics for use in fermentation processes for second-generation ethanol production, highlighting the importance of bioprospecting research of micro-organisms for biotechnological applications. This study widens the scope for future researches that may examine the native yeasts presented, as limited studies have investigated these species previously.

ACS Style

R.M. Cadete; M.A. Melo-Cheab; Kelly Dussan; Rita De Cássia Lacerda Brambilla Rodrigues; Silvio Silvério da Silva; F.C.O. Gomes; C.A. Rosa. Production of bioethanol in sugarcane bagasse hemicellulosic hydrolysate byScheffersomyces parashehatae,Scheffersomyces illinoinensisandSpathaspora arborariaeisolated from Brazilian ecosystems. Journal of Applied Microbiology 2017, 123, 1203 -1213.

AMA Style

R.M. Cadete, M.A. Melo-Cheab, Kelly Dussan, Rita De Cássia Lacerda Brambilla Rodrigues, Silvio Silvério da Silva, F.C.O. Gomes, C.A. Rosa. Production of bioethanol in sugarcane bagasse hemicellulosic hydrolysate byScheffersomyces parashehatae,Scheffersomyces illinoinensisandSpathaspora arborariaeisolated from Brazilian ecosystems. Journal of Applied Microbiology. 2017; 123 (5):1203-1213.

Chicago/Turabian Style

R.M. Cadete; M.A. Melo-Cheab; Kelly Dussan; Rita De Cássia Lacerda Brambilla Rodrigues; Silvio Silvério da Silva; F.C.O. Gomes; C.A. Rosa. 2017. "Production of bioethanol in sugarcane bagasse hemicellulosic hydrolysate byScheffersomyces parashehatae,Scheffersomyces illinoinensisandSpathaspora arborariaeisolated from Brazilian ecosystems." Journal of Applied Microbiology 123, no. 5: 1203-1213.