Pakistan is facing a severe energy crisis due to its heavy dependency on the import of costly fossil fuels, which ultimately leads to expansive electricity generation, a low power supply, and interruptive load shedding. In this regard, the utilization of available renewable energy resources within the country for production of electricity can lessen this energy crisis. Livestock waste/manure is considered the most renewable and abundant material for biogas generation. Pakistan is primarily an agricultural country, and livestock is widely kept by the farming community, in order to meet their needs. According to the 2016–2018 data on the livestock population, poultry held the largest share at 45.8%, followed by buffaloes (20.6%), cattle (12.7%), goats (10.8%), sheep (8.4%), asses (1.3%), camels (0.25%), horses (0.1%), and mules (0.05%). Different animals produce different amounts of manure, based upon their size, weight, age, feed, and type. The most manure is produced by cattle (10–20 kg/day), while poultry produce the least (0.08–0.1 kg/day). Large quantities of livestock manure are produced from each province of Pakistan; Punjab province was the highest contributor (51%) of livestock manure in 2018. The potential livestock manure production in Pakistan was 417.3 million tons (Mt) in 2018, from which 26,871.35 million m³ of biogas could be generated—with a production potential of 492.6 petajoules (PJ) of heat energy and 5521.5 MW of electricity. Due to its favorable conditions for biodigester technologies, and through the appropriate development of anaerobic digestion, the currently prevailing energy crises in Pakistan could be eliminated.

The production of β-mannanase from palm kernel cake (PKC) as a substrate in solid substrate fermentation (SSF) was studied using a laboratory column bioreactor. The simultaneous effects of three independent variables, namely incubation temperature, initial moisture content of substrate and airflow rate, on β-mannanase production were evaluated by response surface methodology (RSM) on the basis of a central composite face-centered (CCF) design. Eighteen trials were conducted in which Aspergillus niger FTCC 5003 was cultivated on PKC in an aerated column bioreactor for seven days under SSF process. The highest level of β-mannanase (2117.89 U/g) was obtained when SSF process was performed at incubation temperature, initial moisture level and aeration rate of 32.5°C, 60% and 0.5 l/min, respectively. Statistical analysis revealed that the quadratic terms of incubation temperature and initial moisture content had significant effects on the production of β-mannanase (P < 0.01). A similar analysis also demonstrated that the linear effect of initial moisture level and an interaction effect between the initial moisture content and aeration rate significantly influenced the production of β-mannanase (P < 0.01). The statistical model suggested that the optimal conditions for attaining the highest level of β-mannanase were incubation temperature of 32°C, initial moisture level of 59% and aeration rate of 0.5 l/min. A β-mannanase yield of 2231.26 U/g was obtained when SSF process was carried out under the optimal conditions described above.