Effects of substrate and water depth on the physiological status of a submerged macrophyte,Vallisneria natans(Lour.) H. Hara, were determined by measuring biomarkers in leaves and roots, to understand factors limiting the re-establishment ofV. natansin urban eutrophic ponds. Ramets ofV. natanswere grown in the laboratory using aquaria containing water and bottom mud from a eutrophic pond and maintained under sufficient light in an incubator. The growth and chlorophyll-a (Chl-a) content of leaves were greater in aquaria with mud than in those with sand, which was used as the reference substrate. The contents of a peroxidation product (malondialdehyde (MDA)) and three antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD)) in leaves and roots, used as stress biomarkers, changed during the experiment, although differences in these contents between mud and sand were not consistent across the experimental days. To control water depth in the field, ramets ofV. natanswere grown in cages with different substrates (mud and sand) installed at different depths (0.5, 1.2, and 2.0 m) in the pond. The mean light quantum during the experiment decreased with increasing depth, from 79.3 μmol/m²s at 0.5 m to 7.9 μmol/m²s at 2.0 m. The Chl-a content in leaves decreased, whereas the MDA content in both leaves and roots increased with increasing water depth. All enzyme activities increased at the beginning and then decreased to the end of the experiment at 2.0 m depth, suggesting deterioration of enzyme activities due to depth-related stress. The MDA content and CAT activity were higher for sand than for mud, whereas the difference in the growth and the leaf Chl-a content between substrates remained unclear in the pond. On comparing the laboratory and field experiments, the leaf Chl-a content was found to be lower and the MDA content and enzyme activities exhibited sharp increase for ramets grown in the pond, even at 0.5 m depth, when compared with those grown in the aquaria. Our results suggest that the bottom mud of the pond is not the major limiting factor in the re-establishment ofV. natans. Because water depth and light attenuation exerted strong stress onV. natans, shallow areas or measures to improve water transparency are required to promote the introduction ofV. natansin eutrophic ponds for successful restoration in urban areas.

Riverine ponds, which are formed and sustained through sediment erosion and deposition, are key habitats for enhancing biodiversity in river reaches. The objective of this study was to understand the roles of traditional river-training wooden structures called "seigyu" on the formation of ponds on nonvegetated bars. Here, the spatial and temporal patterns of the flow and bedform coupled with pond formations for several flood events were assessed. The surface flow patterns were monitored by an unmanned aerial vehicle (UAV) and evaluated by large-scale particle image velocimetry (LSPIV); the maximum flow velocities were 1.3 m s^-1 and 1.9 m s^-1 during floods when seigyu units were partially and fully submerged, respectively. Although the overall mean flow velocity was greater for the latter events, the spatial variation in flow velocity and dissipation rate of turbulent kinetic energy (TKE) around seigyu was greater for the former events. Such flow patterns affected both bed formation and ecological habitats; ponds were formed at locations beside and behind seigyu, where the flow converged and bed scouring occurred during floods. The frequency and size of ponds around seigyu increased in the early half of the season, and they decreased in the other half when floods were greater in magnitude, which suggests that the bed scouring effect of seigyu was greater in flood stages with partial than fully submergence. Although the bar ponds lack shade to temper the effects of incident light and atmospheric conditions, the ponds displayed smaller daily oscillations in temperature than did the main river, probably due to hyporheic water supply to the ponds. Because many aquatic species cannot tolerate extremely high temperatures in summer, the generation and maintenance of deeper ponds by scouring with sufficient water exchange with the hyporheic zone can be key to enhancing colonization by various aquatic species.

To understand the potential roles of terrestrial bamboo on controlling cyanobacterial blooms in aquatic systems, growth rates of the cyanobacterium Microcystis aeruginosa and its competitor algae were examined under different concentrations of bamboo extract. In mono-species cultures with unicellular algal strains, 5.0 g L⁻¹ extract treatment suppressed M. aeruginosa growth, while it had little effect on the growth of green alga Scenedesmus obliquus or promoted the growth of diatom Nitzschia palea. In co-species cultures, the extract treatment increased the effect of S. obliquus and N. palea on the growth of M. aeruginosa. Under the extract treatment with a field-collected M. aeruginosa population, its cell density declined and its colony was etiolated and sank, while co-cultured N. palea increased explosively by invading the colony. These results suggest that bamboo forest stands along banks and artificially installed bamboo poles can affect the aquatic environment for phytoplankton community. Enhancing the growth of competitors, especially diatoms that can invade cyanobacterial colonies, by using extracts or by providing substrates for growth, was suggested to be the major way of the bloom control by bamboo.

Surface aerators have often been introduced in urban ponds for esthetics, but their roles in remediating water quality are less understood. Effects of surface aerators on controlling eutrophication were examined in two urban ponds, in which anaerobic odors and cyanobacterial blooms had occurred and several aerators had been installed. In one of the ponds, a dramatic improvement in dissolved oxygen (DO) (from 1.8 to 8.1 mg L⁻¹) and total phosphorus (TP) (from 1.6 to 0.4 mg L⁻¹) was evident based on the comparison before and after the aeration. Although cyano-bloom did not occur, phytoplankton was dominated by cyanobacteria Microcystis species in both periods. Chlorophyll a (Chl-a) increased (from 29 to 51 μg L⁻¹) and water transparency decreased (from 81 to 27 cm) after the aeration. In the other pond with an irregular shape, water quality was monitored two years after the installation to examine seasonal variation in trophic state and its spatial variation associated with aerator distribution. The water was mixed vertically well for the whole pond, as indicated by small surface-to-bottom differences in temperature. DO decreased in summer after rainfall but was always >5.7 mg L⁻¹. Total nitrogen (TN) and TP were 0.8–2.3 and 0.03–0.07 mg L⁻¹, respectively, and no cyano-blooms were observed across sites throughout the year. Phytoplankton was dominated by green algae and diatom species, which may be favored by the lower phosphorus level of the pond. Chl-a was higher and transparency was lower in the north side, which had more aerators and less shade from trees and buildings. These results suggest that surface aerators increased DO by vertical and horizontal mixing of water, reduced phosphorus release from sediment, and prevented cyano-bloom occurrence, but they did not improve Chl-a level and transparency. Rather, aeration can promote algal growth, and thus, additional purifying measures such as filtration and contact oxidation are required to further improve the trophic state of these ponds.