Oxygen (O₂) is the most crucial substrate for numerous biochemical processes in plants. Its deprivation is a critical factor that affects plant growth and may lead to death if it lasts for a long time. However, various biotic and abiotic factors cause O₂ deprivation, leading to hypoxia and anoxia in plant tissues. To survive under hypoxia and/or anoxia, plants deploy various mechanisms such as fermentation paths, reactive oxygen species (ROS), reactive nitrogen species (RNS), antioxidant enzymes, aerenchyma, and adventitious root formation, while nitrate (NO₃⁻), nitrite (NO₂⁻), and nitric oxide (NO) have shown numerous beneficial roles through modulating these mechanisms. Therefore, in this review, we highlight the role of reductive pathways of NO formation which lessen the deleterious effects of oxidative damages and increase the adaptation capacity of plants during hypoxia and anoxia. Meanwhile, the overproduction of NO through reductive pathways during hypoxia and anoxia leads to cellular dysfunction and cell death. Thus, its scavenging or inhibition is equally important for plant survival. As plants are also reported to produce a potent greenhouse gas nitrous oxide (N₂O) when supplied with NO₃⁻ and NO₂⁻, resembling bacterial denitrification, its role during hypoxia and anoxia tolerance is discussed here. We point out that NO reduction to N₂O along with the phytoglobin-NO cycle could be the most important NO-scavenging mechanism that would reduce nitro-oxidative stress, thus enhancing plants’ survival during O₂-limited conditions. Hence, understanding the molecular mechanisms involved in reducing NO toxicity would not only provide insight into its role in plant physiology, but also address the uncertainties seen in the global N₂O budget.

Paddies are a potential source of anthropogenic nitrous oxide (N₂O) emission. In paddies, both the soil and the rice plants emit N₂O into the atmosphere. The rice plant in the paddy is considered to act as a channel between the soil and the atmosphere for N₂O emission. However, recent studies suggest that plants can also produce N₂O, while the mechanism of N₂O formation in plants is unknown. Consequently, the rice plant is only regarded as a channel for N₂O produced by soil microorganisms. The emission of N₂O by aseptically grown plants and the distinct dual isotopocule fingerprint of plant-emitted N₂O, as reported by various studies, support the production of N₂O in plants. Herein, we propose a potential pathway of N₂O formation in the rice plant. In rice plants, N₂O might be formed in the mitochondria via the nitrate–nitrite–nitric oxide (NO₃–NO₂–NO) pathway when the cells experience hypoxic or anoxic stress. The pathway is catalyzed by various enzymes, which have been described. So, N₂O emitted from paddies might have two origins, namely soil microorganisms and rice plants. So, regarding rice plants only as a medium to transport the microorganism-produced N₂O might be misleading in understanding the role of rice plants in the paddy. As rice cultivation is a major agricultural activity worldwide, not understanding the pathway of N₂O formation in rice plants would create more uncertainties in the N₂O budget.

Background: The compaction of subsoils in agriculture is a threat to soil functioning. Measures aimed at the prevention, amelioration, and/or impact alleviation of compacted subsoils have been studied for more than a century, but less in smallholder agriculture. Methods: A meta-analysis was conducted to quantitatively examine the effects of the prevention, amelioration, and impact alleviation measures in mechanized and small-holder agriculture countries, using studies published during 2000~2019/2020. Results: Mean effect sizes of crop yields were large for controlled traffic (+34%) and irrigation (+51%), modest for subsoiling, deep ploughing, and residue return (+10%), and negative for no-tillage (−6%). Mean effect sizes of soil bulk density were small (<10%), suggesting bulk density is not a sensitive ‘state’ indicator. Mean effect sizes of penetration resistance were relatively large, with large variations. Controlled traffic had a larger effect in small-holder farming than mechanized agriculture. Conclusion: We found no fundamental differences between mechanized and smallholder agriculture in the mean effect sizes of the prevention, amelioration, and impact alleviation measures. Measures that prevent soil compaction are commonly preferred, but amelioration and alleviation are often equally needed and effective, depending on site-specific conditions. A toolbox of soil compaction prevention, amelioration, and alleviation measures is needed, for both mechanized and smallholder agriculture.

Current tillage practices in the important winter wheat–summer maize double cropping system of the North China Plain are under debate because of negative effects on soil quality and crop yield. Therefore, a long-term experiment was conducted from 2001 to 2018 to determine the effects of soil conservation practices on crop yield and soil quality. The treatments were imposed following maize harvest and prior wheat seeding, and were defined as follows: (1) moldboard ploughing (0–20 cm) following maize straw removal (CK); (2) moldboard ploughing (0–20 cm) following maize straw return (CT); (3) rotary tillage following maize straw return (RT); and (4) no tillage with maize straw covering the soil surface (NT). Wheat straw was chopped and spread on the soil in all treatments and maize seeded without prior tillage. Wheat yields were higher in CT than RT and NT treatments (p < 0.05); NT had 18% lower wheat yields than CT. No significant differences were found between treatments in summer maize yields. The soil organic carbon (SOC) content in the surface layer (0–5 cm) was higher in NT and RT compared to CT and CK. However, SOC content in the 10–20 cm and 20–30 cm layers was lower in NT and RT compared to CT and CK. Similarly, available phosphorus in the surface soil was higher in NT and RT than in CT and CK. but the opposite was true for the lower soil layers. SOC stocks (0–30 cm) increased in all treatments, and were initially faster in NT and RT than in CT and CK. However, SOC stocks were higher in CT than in other treatments at the end of the experiment. This finding indicates that no tillage and reduced tillage decreased both wheat yields and soil C sequestration over time; it also indicates that CT was the most robust in terms of crop yields and soil C sequestration.