The methods for estimating methane emissions from cattle as used in the Australian national inventory are based on older data that have now been superseded by a large amount of more recent data. Recent data suggested that the current inventory emissions estimates can be improved. To address this issue, a total of 1034 individual animal records of daily methane production (MP) was used to reassess the relationship between MP and each of dry matter intake (DMI) and gross energy intake (GEI). Data were restricted to trials conducted in the past 10 years using open-circuit respiration chambers, with cattle fed forage-based diets (forage >70%). Results from diets considered to inhibit methanogenesis were omitted from the dataset. Records were obtained from dairy cattle fed temperate forages (220 records), beef cattle fed temperate forages (680 records) and beef cattle fed tropical forages (133 records). Relationships were very similar for all three production categories and single relationships for MP on a DMI or GEI basis were proposed for national inventory purposes. These relationships were MP (g/day) = 20.7 (±0.28) × DMI (kg/day) (R2 = 0.92, P < 0.001) and MP (MJ/day) = 0.063 (±0.008) × GEI (MJ/day) (R2 = 0.93, P < 0.001). If the revised MP (g/day) approach is used to calculate Australia’s national inventory, it will reduce estimates of emissions of forage-fed cattle by 24%. Assuming a global warming potential of 25 for methane, this represents a 12.6 Mt CO2-e reduction in calculated annual emissions from Australian cattle.

Aedes aegypti mosquitoes infected with the wMel strain of Wolbachia are being released into natural mosquito populations in the tropics as a way of reducing dengue transmission. High temperatures adversely affect wMel, reducing Wolbachia density and cytoplasmic incompatibility in some larval habitats that experience large temperature fluctuations. We monitored the impact of a 43.6°C heatwave on the wMel infection in a natural population in Cairns, Australia, where wMel was first released in 2011 and has persisted at a high frequency. Wolbachia infection frequencies in the month following the heatwave were reduced to 83% in larvae sampled directly from field habitats and 88% in eggs collected from ovitraps, but recovered to be near 100% four months later. Effects of the heatwave on wMel appeared to be stage-specific and delayed, with reduced frequencies and densities in field-collected larvae and adults reared from ovitraps but higher frequencies in field-collected adults. Laboratory experiments showed that the effects of heatwaves on cytoplasmic incompatibility and density are life stage-specific, with first instar larvae being the most vulnerable to temperature effects. Our results indicate that heatwaves in wMel-infected populations will have only temporary effects on Wolbachia frequencies and density once the infection has established in the population. Our results are relevant to ongoing releases of wMel-infected Ae. aegypti in several tropical countries. Mosquitoes infected with Wolbachia bacteria are being released in the tropics to replace natural mosquito populations and suppress dengue transmission. Aedes aegypti mosquitoes with the wMel strain of Wolbachia were first released in Cairns, Australia in 2011 and releases were then expanded to the entire city and surrounding suburbs. Today, wMel is at a high frequency within the Ae. aegypti population and local dengue transmission in Cairns has declined to nearly zero. Wolbachia infections are vulnerable to high temperatures and the ability of wMel to persist in populations and block dengue may be constrained by climate. Cairns experienced a record heatwave of 43.6°C in November 2018 and we wanted to see whether this affected the wMel-infected Ae. aegypti population. Our results show that the frequency and density of wMel declined after the heatwave, with effects depending on the mosquito life stage tested. When we monitored the population again in April 2019, wMel had returned to a high frequency. We suggest that heatwaves of the magnitude experienced in Cairns will not have long-term impacts on the wMel infection but may affect invasion during releases or interfere with dengue blockage. Heatwaves may affect interventions with wMel-infected Ae. aegypti that are being deployed in several countries. Effects may depend on the proportion of larval habitats that are protected from extreme temperature fluctuations.

Background: Aedes albopictusis a highly invasive species and an important vector of dengue and chikungunya viruses. Indigenous to Southeast Asia,Ae. albopictushas successfully invaded every inhabited continent, except Antarctica, in the past 80 years. Vector surveillance and control at points of entry (PoE) is the most critical front line of defence against the introduction ofAe. albopictusto new areas. Identifying the pathways by whichAe. albopictusare introduced is the key to implementing effective vector surveillance to rapidly detect introductions and to eliminate them.Methods: A literature review was conducted to identify studies and data sources reporting the known and suspected dispersal pathways of human-mediatedAe. albopictusdispersal between 1940–2020. Studies and data sources reporting the first introduction ofAe. albopictusin a new country were selected for data extraction and analyses.Results: Between 1940–2020,Ae. albopictuswas reported via various dispersal pathways into 86 new countries. Two main dispersal pathways were identified: (1) at global and continental spatial scales, maritime sea transport was the main dispersal pathway forAe. albopictusinto new countries in the middle to late 20th Century, with ships carrying used tyres of particular importance during the 1980s and 1990s, and (2) at continental and national spatial scales, the passive transportation ofAe. albopictusin ground vehicles and to a lesser extent the trade of used tyres and maritime sea transport appear to be the major drivers ofAe. albopictusdispersal into new countries, especially in Europe. Finally, the dispersal pathways for the introduction and spread ofAe. albopictusin numerous countries remains unknown, especially from the 1990s onwards.Conclusions: This review identified the main known and suspected dispersal pathways of human-mediatedAe. albopictusdispersal leading to the first introduction ofAe. albopictusinto new countries and highlighted gaps in our understanding ofAe. albopictusdispersal pathways. Relevant advances in vector surveillance and genomic tracking techniques are presented and discussed in the context of improving vector surveillance.Graphical

With the impending threat of climate change, greater understanding of patterns of species distributions and richness and the environmental factors driving them are required for effective conservation efforts. Species distribution models enable us to not only estimate geographic extents of species and subsequent patterns of species richness, but also generate hypotheses regarding environmental factors determining these spatial patterns. Projected changes in climate can then be used to predict future patterns of species distributions and richness. We created distribution models for most of the flightless ground beetles (Carabidae) within the Wet Tropics World Heritage Area of Australia, a major component of regionally endemic invertebrates. Forty-three species were modelled and the environmental correlates of these distributions and resultant patterns of species richness were examined. Flightless ground beetles generally inhabit upland areas characterised by stable, cool and wet environmental conditions. These distribution and richness patterns are best explained using the time-stability hypothesis as this group’s primary habitat, upland rainforest, is considered to be the most stable regional habitat. Projected changes in distributions indicate that as upward shifts in distributions occur, species currently confined to lower and drier mountain ranges will be more vulnerable to climate change impacts than those restricted to the highest and wettest mountains. Distribution models under projected future climate change suggest that there will be reductions in range size, population size and species richness under all emission scenarios. Eighty-eight per cent of species modelled are predicted to decline in population size by over 80%, for the most severe emission scenario by the year 2080. These results suggest that flightless ground beetles are among the most vulnerable taxa to climate change impacts so far investigated in the Wet Tropics World Heritage Area. These findings have dramatic implications for all other flightless insect taxa and the future biodiversity of this region.

The threat of anthropogenic climate change has seen a renewed focus on understanding contemporary patterns of species distribution. This is especially the case for the biota of tropical mountains, because tropical species often have particularly narrow elevational ranges and there are high levels of short-range endemism. Here we describe geographic patterns of ant diversity and distribution in the World Heritage-listed rainforests of the Australian Wet Tropics (AWT), revealing seasonal moisture stability to be an important environmental correlate of elevational patterns of species composition. We sampled ants in leaf litter, on the litter surface and on tree trunks at 26 sites from six subregions spanning five degrees of latitude and elevation ranges from 100–1,300 m. A total of 296 species from 63 genera were recorded. Species richness showed a slight peak at mid elevations, and did not vary significantly with latitude. Species composition varied substantially between subregions, and many species have highly localised distributions. There was very marked species turnover with elevation, with a particularly striking compositional disjunction between 600 m and 800 m at each subregion. This disjunction coincides with a strong environmental threshold of seasonal stability in moisture associated with cloud ‘stripping’. Our study therefore provides further support for climatic stability as a potential mechanism underlying patterns of diversity. The average height of orographic cloud layers is predicted to rise under global warming, and associated shifts in seasonal moisture stability may exacerbate biotic change caused by rising temperature alone.