Curculigo orchioides and C. latifolia have been used as traditional medicines such as antidiabetic and anticancer. This study measured the total phenolics and flavonoid contents as well as analyzed the functional groups and chemical compounds using Fourier-transform infrared (FTIR) spectra and UHPLC-Q-Orbitrap-HRMS profiling for the discrimination of plant parts, geographical origin, and compounds that presumably have a significant contribution as antioxidant and α-glucosidase inhibitors on both plants. The total phenolics and flavonoids contents in Curculigo species varied from 142.09 to 452.47 mg gallic acid equivalent (GAE/g) and from 0.82 to 5.44 mg quercetin equivalent (QE/g), respectively. The lowest IC₅₀ for antioxidant and α-glucosidase inhibitory activities is presented by C. latifolia from a higher altitude region. Principal component analysis (PCA) from FTIR and UHPLC-Q-Orbitrap-HRMS data could discriminate the plant parts and geographical origin. Partial least squares (PLS) analysis has identified several functional groups, such as O–H, C–H, C=O, C–C, C–O, and chemical compounds, unknown-185 and unknown-85, that are most likely to contribute to the antioxidant and α-glucosidase inhibitory activities.

Curculigo spp. is a herb that is commonly used in Indonesia to treat diabetes mellitus (DM) . The main active components of Curculigo spp. were identified through our previous metabolomic study and online database platform. However, the biological mechanisms underlying Curculigo spp. activity in treating DM remain unclear. Therefore, in this study, a network pharmacology was used to explore the active compounds of Curculigo spp. and their potential molecular mechanisms for treating DM. Oral bioavailability and drug-likeness from the compounds of Curculigo spp. were screened using Lipinski’s rule of five, BBB, HIA + and Caco-2 permeability criteria. A network of compound–target–disease–pathway was then constructed using Cytoscape. The highest degree compounds and targets were then confirmed by molecular docking and molecular dynamics (MD) simulations. The human body can absorb 33 compounds derived from Curculigo spp. In addition, 58 nodes and 62 edges generated a network analysis with the DM target. The highest degree of the compound–target–disease pathway was for orcinol glucoside, AKR1B1, autoimmune diabetes, bile acid and bile salt metabolism. Furthermore, the computational docking method on Curculigo spp. compounds with the highest degree revealed that orcinol glucoside interacted with PTPN1 through a hydrogen bond and resulted in a binding energy of −7.2 kcal mol⁻¹. Through hydrogen bonds, orcinol glucoside in PTPN1 regulates multiple signaling pathways via the adherens junction pathway, which may play a therapeutic role in DM (type 2 diabetes: obesity). In addition, MD simulation confirmed that orcinol glucoside, is suitable for DM treatment by interacting with PTPN1. Communicated by Ramaswamy H. Sarma

Mulberry (Morus alba L.) leaf has been used as a traditional medicine for treatment of some tropical diseases and also popular using for silkworm feed. On this research, anti-inflammatory and wound healing activities of the bark extract has been investigated in vivo. The bark was extracted with three organic solvent in a different polarity to carry out a group compounds in n-hexane extract (EH), Eto-Ac extract (EE), and EtOH extract (EO). Anti-inflammatory was investigated on inflammatory mice induced by 1% carrageenan. Among the extract (200mg/kg BW), EE showed strongly decrease inflammation by 42% than the other extract and stronger than the positive control, Na-diclofenac. Further investigation on healing effect of wound and burn injury which was tested at concentration of 1% topically to each extract. All the extract significantly showed healing activity, among them EE was observed as the stronger one while slightly less than the positive control. This result indicated that the most active compounds on anti-inflammatory and healing wounded was extracted with ethyl acetate that could be used for drug discovery.