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Head of the Clinic of Surgical and Oncologic Gynecology, First Department of Gynecology and Obstetrics, Medical University of Lodz, Poland; specialist in gynecology and obstetrics and gynecological oncology; https://orcid.org/0000-0002-2072-6533
Background and Objectives: Uterine fibroids develop in 25–40% of women of childbearing age; however, there are discrepancies resulting from population and socioeconomic differences. The pathogenesis of fibroids is not clear. The aim of the study was to assess the potential connection between the use of oral contraceptives and the occurrence of uterine fibroids in women of childbearing age. Materials and Methods: In this prospective, survey, case–control study, data were collected from Caucasian female patients (mean age = 30) using a questionnaire concerning the onset, duration and form of hormonal contraception, and medical and obstetrical history. The questionnaires were handed personally to hospitalized patients as well as distributed through Google forms on social media. Results: In a study group (n = 140) of patients using hormonal contraception, 37.8% of them were diagnosed with uterine fibroids, whereas among the patients not using hormonal contraception (n = 206), uterine fibroids were diagnosed in 59.6% of the patients. The most common hormonal contraception was two-component hormonal tablets used by 93.3% of the patients. Taking contraceptives was a uterine fibroids protective factor (OR = 0.4, p = 0.007). In the study group, 5.5% of the patients were pregnant and 60.42% were diagnosed with uterine fibroids (OR = 4.4, p< 0.000001). Conclusion: Contraception was found to be a protective factor for uterine fibroids among the women surveyed. The presented data confirm the theory about the hormonal dependence of uterine fibroids.
Katarzyna Kwas; Aleksandra Nowakowska; Angelika Fornalczyk; Magda Krzycka; Anna Nowak; Jacek Wilczyński; Maria Szubert. Impact of Contraception on Uterine Fibroids. Medicina 2021, 57, 717 .
AMA StyleKatarzyna Kwas, Aleksandra Nowakowska, Angelika Fornalczyk, Magda Krzycka, Anna Nowak, Jacek Wilczyński, Maria Szubert. Impact of Contraception on Uterine Fibroids. Medicina. 2021; 57 (7):717.
Chicago/Turabian StyleKatarzyna Kwas; Aleksandra Nowakowska; Angelika Fornalczyk; Magda Krzycka; Anna Nowak; Jacek Wilczyński; Maria Szubert. 2021. "Impact of Contraception on Uterine Fibroids." Medicina 57, no. 7: 717.
The aim of this review is to clarify the relative association between adenomyosis and infertility and the possible treatment for an infertile patient. Although adenomyosis is detected more often in women of late reproductive age, its influence on pregnancy rates is important, especially considering the tendency to delay pregnancy among women in developed countries. In this article, we present a critical analysis of the literature data concerning the impact of adenomyosis on fertility. The possible effects of treatment on the pregnancy rate will also be discussed. We conducted a literature search; publications from Pubmed, Embase and Cochrane databases published from 1982 to 2019 were retrieved using terms ’adenomyosis and infertility’ and ’adenomyosis and pregnancy outcomes’, extensively studied in the aspects of diagnosis, pathogenesis of infertility and possible treatment methods. Molecular studies have given deep insight into the pathogenesis of adenomyosis in the recent few years, but there is a huge discrepancy between in vitro studies and praxis. Oral contraceptive pills, anti-prostaglandins, oral or parenteral progestins, danazol and gonadotrophin-releasing hormone (GnRH) analogues have all been used to control menstrual pain and menorrhagia in women with adenomyosis, but they temporarily suppress the menstrual cycle. Additionally, endometrial ablation and hysterectomy used to alleviate pain caused by adenomyosis exclude pregnancy planning. The development of imaging techniques—ultrasound and MRI—enables the diagnosis of adenomyosis with very high accuracy nowadays, but the methods of treatment mentioned above have not given satisfactory results in women planning pregnancy. For these patients, the high-intensity-focused ultrasound method (HIFU) and combined treatment before assisted reproductive techniques can prove beneficial in adenomyosis patients.
Maria Szubert; Edward Koziróg; Olga Olszak; Klaudia Krygier-Kurz; Jakub Kazmierczak; Jacek Wilczynski. Adenomyosis and Infertility—Review of Medical and Surgical Approaches. International Journal of Environmental Research and Public Health 2021, 18, 1235 .
AMA StyleMaria Szubert, Edward Koziróg, Olga Olszak, Klaudia Krygier-Kurz, Jakub Kazmierczak, Jacek Wilczynski. Adenomyosis and Infertility—Review of Medical and Surgical Approaches. International Journal of Environmental Research and Public Health. 2021; 18 (3):1235.
Chicago/Turabian StyleMaria Szubert; Edward Koziróg; Olga Olszak; Klaudia Krygier-Kurz; Jakub Kazmierczak; Jacek Wilczynski. 2021. "Adenomyosis and Infertility—Review of Medical and Surgical Approaches." International Journal of Environmental Research and Public Health 18, no. 3: 1235.
Despite the nondisputable progress in the treatment of ovarian cancer, it still brings a serious challenge to doctors and poor prognosis to the majority of patients. The most important processes, which shape cancer development, are neo-angiogenesis and chronic inflammation. Mediators engaged in their regulation have a deep impact on the ovarian cancer proliferation, peritoneal spread, and distant metastases. The regulatory network, which contributes to cancer escape from host immunosurveillance, is very complex and still needs to be fully uncovered. However, it’s so far understanding has brought some improvements in ovarian cancer therapy. One of them is the introduction of Bevacizumab into routine treatment, based on its clinical efficacy. It is hoped that information presented in this chapter will help to summarize the present knowledge about immunology of ovarian cancer and to fertilize the reader’s mind with new ideas.
Jacek R. Wilczyński; Marek Nowak; Miłosz Wilczyński. Immunology and Immunotherapy of Ovarian Cancer. Cancer Immunology 2020, 487 -540.
AMA StyleJacek R. Wilczyński, Marek Nowak, Miłosz Wilczyński. Immunology and Immunotherapy of Ovarian Cancer. Cancer Immunology. 2020; ():487-540.
Chicago/Turabian StyleJacek R. Wilczyński; Marek Nowak; Miłosz Wilczyński. 2020. "Immunology and Immunotherapy of Ovarian Cancer." Cancer Immunology , no. : 487-540.
Marta Kostrzewa; Ewa Głowacka; Tomasz Stetkiewicz; Mariusz Grzesiak; Krzysztof Szyłło; Grzegorz Stachowiak; Jacek Wilczyński. Is serum anti-Müllerian hormone (AMH) assay a satisfactory measure for ovarian reserve estimation? A comparison of serum and peritoneal fluid AMH levels. Advances in Clinical and Experimental Medicine 2020, 29, 853 -856.
AMA StyleMarta Kostrzewa, Ewa Głowacka, Tomasz Stetkiewicz, Mariusz Grzesiak, Krzysztof Szyłło, Grzegorz Stachowiak, Jacek Wilczyński. Is serum anti-Müllerian hormone (AMH) assay a satisfactory measure for ovarian reserve estimation? A comparison of serum and peritoneal fluid AMH levels. Advances in Clinical and Experimental Medicine. 2020; 29 (7):853-856.
Chicago/Turabian StyleMarta Kostrzewa; Ewa Głowacka; Tomasz Stetkiewicz; Mariusz Grzesiak; Krzysztof Szyłło; Grzegorz Stachowiak; Jacek Wilczyński. 2020. "Is serum anti-Müllerian hormone (AMH) assay a satisfactory measure for ovarian reserve estimation? A comparison of serum and peritoneal fluid AMH levels." Advances in Clinical and Experimental Medicine 29, no. 7: 853-856.
The aim of this study was to evaluate the knowledge regarding a healthy lifestyle and prophylaxis during pregnancy among women from rural and urban areas and how this changed within a 5-year period. Analyses of the population of pregnant women in Poland were made using a questionnaire survey. The survey was conducted in the years 2010–2012 and 2017. Questionnaires from 6128 pregnant women were collected. The statistical analyses were conducted using IBM SPSS. The examined population was comprised of 41% women from rural areas and 59% women from urban areas. Alcohol consumption was lower among women from rural areas than among urban inhabitants in 2010–2012; in 2017 a trend of even lower consumption was observed. Folic acid supplementation was more broadly developed in the urban population; however, in 2017, higher percentage rates of both populations admitted taking folates before pregnancy. More women in urban than in rural areas performed physical activity during pregnancy, but the differences decreased in 2017. Knowledge of a healthy lifestyle and prophylaxis during pregnancy increased regardless of place of residence; however, the most evident change could be observed among women from rural areas.
Maria Szubert; Malwina Ilowiecka; Jacek Wilczynski; Przemyslaw Bilinski; Cezary Wojtyla. Health-Related Behaviors of Pregnant Women Residing in Urban and Rural Areas in Poland. International Journal of Environmental Research and Public Health 2020, 17, 4395 .
AMA StyleMaria Szubert, Malwina Ilowiecka, Jacek Wilczynski, Przemyslaw Bilinski, Cezary Wojtyla. Health-Related Behaviors of Pregnant Women Residing in Urban and Rural Areas in Poland. International Journal of Environmental Research and Public Health. 2020; 17 (12):4395.
Chicago/Turabian StyleMaria Szubert; Malwina Ilowiecka; Jacek Wilczynski; Przemyslaw Bilinski; Cezary Wojtyla. 2020. "Health-Related Behaviors of Pregnant Women Residing in Urban and Rural Areas in Poland." International Journal of Environmental Research and Public Health 17, no. 12: 4395.
Purpose Approximately 50% of men reporting to clinics for assisted reproduction have abnormal sperm parameters; we therefore considered whether they differ from fertile males in terms of the frequency of KIR and HLA-C genes, suggesting the involvement of NK cells and some T cells in the inflammatory reaction that can occur in the testes, vas deferens, or epididymis. Method We tested a total of 1064 men: 445 of them were patients who, together with their female partners, participated in in vitro fertilization (IVF), 298 men whose female partners suffered from recurrent spontaneous abortion. Three hundred twenty-one fertile men constituted the control group. KIRs were genotyped using KIR Ready Gene kits and HLA-C by PCR-SSP methods. Results We found differences in KIR gene frequencies between men who became fathers via natural conception and men who participated in in vitro fertilization for KIR2DL2 (p/p corr. = 0.0015/0.035, OR = 1.61), KIR2DL5 gr.2 (p/p corr. = 0.0023/0.05, OR = 1.64), KIR2DS2 (p/p corr. = 0.0019/0.044, OR = 1.59), and KIR2DS3 (p/p corr. = 0.0016/0.037, OR = 1.67). KIRs in Cen AA region were significantly overrepresented in fertile males than in IVF males (p/p corr. = 0.0076/0.03, OR = 0.67), whereas Cen AB + Cen BB frequency was higher in IVF males than in fertile males (p/p corr. = 0.0076/0.03, OR = 1.50). We also observed a limited association in KIR-HLA-C combinations. Conclusion Fertile men differ in profile of KIR genes and KIR-HLA-C combinations from men participating in IVF.
Karolina Wilczyńska; Paweł Radwan; Rafał Krasiński; Michał Radwan; Jacek R. Wilczyński; Andrzej Malinowski; Ewa Barcz; Izabela Nowak. KIR and HLA-C genes in male infertility. Journal of Assisted Reproduction and Genetics 2020, 37, 2007 -2017.
AMA StyleKarolina Wilczyńska, Paweł Radwan, Rafał Krasiński, Michał Radwan, Jacek R. Wilczyński, Andrzej Malinowski, Ewa Barcz, Izabela Nowak. KIR and HLA-C genes in male infertility. Journal of Assisted Reproduction and Genetics. 2020; 37 (8):2007-2017.
Chicago/Turabian StyleKarolina Wilczyńska; Paweł Radwan; Rafał Krasiński; Michał Radwan; Jacek R. Wilczyński; Andrzej Malinowski; Ewa Barcz; Izabela Nowak. 2020. "KIR and HLA-C genes in male infertility." Journal of Assisted Reproduction and Genetics 37, no. 8: 2007-2017.
Ovarian cancer (OC) is one of the leading causes of cancer death in women, with high-grade serous ovarian cancer (HGSOC) being the most lethal gynecologic malignancy among women. This high fatality rate is the result of diagnosis of a high number of new cases when cancer implants have already spread. The poor prognosis is due to our inadequate understanding of the molecular mechanisms preceding ovarian malignancy. Knowledge about the site of origination has been improved recently by the discovery of tube intraepithelial cancer (TIC), but the potential risk factors are still obscure. Due to high tumoral heterogeneity in OC, the establishment of early stage biomarkers is still underway. Microbial infection may induce or result in chronic inflammatory infection and in the pathogenesis of cancers. Microbiome research has shed light on the relationships between the host and microbiota, as well as the direct roles of host pathogens in cancer development, progression, and drug efficacy. While controversial, the detection of viruses within ovarian malignancies and fallopian tube tissues suggests that these pathogens may play a role in the development of OC. Genomic and proteomic approaches have enhanced the methods for identifying candidates in early screening. This article summarizes the existing knowledge related to the molecular mechanisms that lead to tumorigenesis in the ovary, as well as the viruses detected in OC cases and how they may elevate this process.
Sudipta Pathak; Jacek R. Wilczyński; Edyta Paradowska. Factors in Oncogenesis: Viral Infections in Ovarian Cancer. Cancers 2020, 12, 561 .
AMA StyleSudipta Pathak, Jacek R. Wilczyński, Edyta Paradowska. Factors in Oncogenesis: Viral Infections in Ovarian Cancer. Cancers. 2020; 12 (3):561.
Chicago/Turabian StyleSudipta Pathak; Jacek R. Wilczyński; Edyta Paradowska. 2020. "Factors in Oncogenesis: Viral Infections in Ovarian Cancer." Cancers 12, no. 3: 561.
Endoplasmic reticulum aminopeptidases ERAP1 and ERAP2 trim peptides to generate stable antigenic epitopes for their presentation by HLA class I (HLA-I) molecules to T cell receptor. By influencing the peptide repertoire of HLA-I molecules, they affect also the interactions of HLA-I with killer cell immunoglobulin-like receptors (KIRs) of natural killer (NK) cells. HLA-C is the only polymorphic HLA-I molecule present on the trophoblast. In this study we investigated the role of ERAP1 and ERAP2 polymorphisms in the context of KIR and HLA-C genes in women suffering from recurrent spontaneous abortion (RSA) in the Polish population. We used TaqMan genotyping assays for ERAP1 rs27044, rs30187, rs2287987, rs26618, rs2665 and ERAP2 rs2248374; PCR-SSP methods for KIR and HLA-C genotyping. We tested 285 women who experienced recurrent spontaneous abortion (RSA) and 319 fertile women. We observed a significant association of ERAP1 rs30187TT genotype with RSA (p = 0.02, OR = 1.89, 95%CI = 1.11–3.21), however the most striking association was found in comparison of patients and controls with ERAP1 rs30187TT and KIR Bx genotypes (p = 0.006, pcorr. = 0.036, OR = 2.40, 95%CI = 1.27–4.52). Moreover, this effect was even stronger in HLA-C2 positive patients (p = 0.0031, pcorr. = 0.019, OR = 3.46, 95%CI = 1.48–8.11). Other weaker associations of the remaining tested ERAP single nucleotide polymorphisms with RSA were also presented. In conclusion, ERAP1 rs30187TT genotype itself increased susceptibility to RSA but this effect was much stronger in patients positive for HLA-C2 and KIR Bx genotypes.
Karolina Wilczyńska; Andrzej Wiśniewski; Andrzej Malinowski; Ewa Barcz; Jacek R. Wilczyński; Piotr Kuśnierczyk; Izabela Nowak. ERAP, KIR and HLA-C gene interaction in susceptibility to recurrent spontaneous abortion in the Polish population. Human Immunology 2019, 80, 344 -348.
AMA StyleKarolina Wilczyńska, Andrzej Wiśniewski, Andrzej Malinowski, Ewa Barcz, Jacek R. Wilczyński, Piotr Kuśnierczyk, Izabela Nowak. ERAP, KIR and HLA-C gene interaction in susceptibility to recurrent spontaneous abortion in the Polish population. Human Immunology. 2019; 80 (5):344-348.
Chicago/Turabian StyleKarolina Wilczyńska; Andrzej Wiśniewski; Andrzej Malinowski; Ewa Barcz; Jacek R. Wilczyński; Piotr Kuśnierczyk; Izabela Nowak. 2019. "ERAP, KIR and HLA-C gene interaction in susceptibility to recurrent spontaneous abortion in the Polish population." Human Immunology 80, no. 5: 344-348.
Reproductive failure in humans is a very important social and economic problem, because nowadays women decide to conceive later in life and delay motherhood. Unfortunately, with increasing age they have less chance for natural fertilization and maintenance of pregnancy. Many of them need assisted reproductive technology. Approximately 10% of women after in vitro fertilization-embryo transfers experience recurrent implantation failure (RIF). Multiple factors may contribute to RIF, including oocyte and sperm quality, parental chromosomal anomalies, genetic or metabolic abnormalities of the embryo, poor uterine receptivity, immunological disturbances in the implantation site, and some gynecologic pathologies such as endometriosis, uterine fibroids, hydrosalpinx and endometrial polyps. Moreover, the procedure of in vitro fertilization itself could adversely influence the implantation. Nowadays, many studies are focused on the role of natural killer (NK) cells in normal and pathologic pregnancy because NK cells constitute the dominant cell population in the endometrium and they come in close contact with the allogeneic extravillous trophoblast cells in early pregnancy decidua. The majority of these cells are of CD56bright phenotype. These cells can express killer immunoglobulin-like receptors (KIRs), which, upon recognition of HLA class I molecules (HLA-C and HLA-G) on trophoblasts, may either stimulate or inhibit NK cells to produce soluble factors, and display low cytotoxicity necessary for maintenance of the allogeneic embryo and fetus in the next steps of pregnancy. Moreover, some members of the leukocyte immunoglobulin-like receptor (LILR) family, also named ILT (immunoglobulin-like transcript), are present in the human placenta. LILRB1 (ILT2) was described mainly on stromal cells, while LILRB2 (ILT4), in addition to stromal cells, was also found around vessels in the smooth muscle layer. In this review we focus on the possible role of polymorphism of KIR, LILRB and their ligands (HLA-C, HLA-G) in susceptibility to recurrent implantation failure, which could serve as diagnostic biomarkers of this disease.
Izabela Nowak; Karolina Wilczyńska; Jacek R. Wilczyński; Andrzej Malinowski; Paweł Radwan; Michał Radwan; Piotr Kuśnierczyk. KIR, LILRB and their Ligands’ Genes as Potential Biomarkers in Recurrent Implantation Failure. Archivum Immunologiae et Therapiae Experimentalis 2017, 65, 391 -399.
AMA StyleIzabela Nowak, Karolina Wilczyńska, Jacek R. Wilczyński, Andrzej Malinowski, Paweł Radwan, Michał Radwan, Piotr Kuśnierczyk. KIR, LILRB and their Ligands’ Genes as Potential Biomarkers in Recurrent Implantation Failure. Archivum Immunologiae et Therapiae Experimentalis. 2017; 65 (5):391-399.
Chicago/Turabian StyleIzabela Nowak; Karolina Wilczyńska; Jacek R. Wilczyński; Andrzej Malinowski; Paweł Radwan; Michał Radwan; Piotr Kuśnierczyk. 2017. "KIR, LILRB and their Ligands’ Genes as Potential Biomarkers in Recurrent Implantation Failure." Archivum Immunologiae et Therapiae Experimentalis 65, no. 5: 391-399.
Despite the nondisputable progress in treatment of ovarian cancer, it still brings serious challenge to the doctors and poor prognosis to the majority of patients. The most important processes which shape the cancer development are neoangiogenesis and chronic inflammation. Mediators engaged in their regulation have a deep impact on the ovarian cancer proliferation, peritoneal spread, and distant metastases. The regulatory network which contributes to cancer escape from host immunosurveillance is very complex and still needs to be fully uncovered: however, its so far understanding has brought some improvements in ovarian cancer therapy. One of them is an introduction of bevacizumab into routine treatment, based on its clinical efficacy. It is hoped that information presented in this chapter will help to summarize the present knowledge about immunology of ovarian cancer and to fertilize the reader’s mind with new ideas.
Jacek R. Wilczyński; Marek Nowak. Immunology and Immunotherapy of Ovarian Cancer. Cancer Immunology 2015, 413 -456.
AMA StyleJacek R. Wilczyński, Marek Nowak. Immunology and Immunotherapy of Ovarian Cancer. Cancer Immunology. 2015; ():413-456.
Chicago/Turabian StyleJacek R. Wilczyński; Marek Nowak. 2015. "Immunology and Immunotherapy of Ovarian Cancer." Cancer Immunology , no. : 413-456.
Emphasizing the dynamic processes between cancer and host immune system, the initially discovered concept of cancer immunosurveillance has been replaced by the current concept of cancer immunoediting consisting of three phases: elimination, equilibrium, and escape. Solid tumors composed of both cancer and host stromal cells are an example of how the three phases of cancer immunoediting functionally evolve, and how a tumor shaped by the host immune system gets a finally resistant phenotype. Elimination, equilibrium, and escape are described in this chapter in detail, including the role of immune surveillance, cancer dormancy, disruption of the antigen-presenting machinery, tumor-infiltrating immune cells, and resistance to apoptosis, as well as the function of tumor stroma, microvesicles, exosomes and inflammation.
Jacek R. Wilczynski; Marek Nowak. Cancer Immunoediting: Elimination, Equilibrium, and Immune Escape in Solid Tumors. Interaction of Immune and Cancer Cells 2013, 143 -205.
AMA StyleJacek R. Wilczynski, Marek Nowak. Cancer Immunoediting: Elimination, Equilibrium, and Immune Escape in Solid Tumors. Interaction of Immune and Cancer Cells. 2013; ():143-205.
Chicago/Turabian StyleJacek R. Wilczynski; Marek Nowak. 2013. "Cancer Immunoediting: Elimination, Equilibrium, and Immune Escape in Solid Tumors." Interaction of Immune and Cancer Cells , no. : 143-205.
The process of chronic inflammation is a common link which connects different kinds of environmental pollutants and infections with tumorigenesis. Transcription factor NF-κB is a common final target for many inflammatory and cell proliferation pathways, independent of the source of stimuli (e.g., cytokines, growth factors, environmental carcinogens, radiation, hypoxia, bacteria, and viruses). Over-activation of NF-κB has been confirmed in many tumors, resulting in worse prognosis for patient survival. Therefore, inhibition of cellular pathways for NF-κB activation is nowadays considered as a promising anti-cancer therapy and is extensively studied in clinical trials, or even has been adopted as an approved therapy in some kinds of cancer.
Jacek Wilczynski; Markus Duechler; Malgorzata Czyz. Targeting NF-κB and HIF-1 Pathways for the Treatment of Cancer: Part I. Archivum Immunologiae et Therapiae Experimentalis 2011, 59, 289 -299.
AMA StyleJacek Wilczynski, Markus Duechler, Malgorzata Czyz. Targeting NF-κB and HIF-1 Pathways for the Treatment of Cancer: Part I. Archivum Immunologiae et Therapiae Experimentalis. 2011; 59 (4):289-299.
Chicago/Turabian StyleJacek Wilczynski; Markus Duechler; Malgorzata Czyz. 2011. "Targeting NF-κB and HIF-1 Pathways for the Treatment of Cancer: Part I." Archivum Immunologiae et Therapiae Experimentalis 59, no. 4: 289-299.
Hypoxia that originates from disturbed growth of solid tumors initiates a cascade of intracellular events engaging hypoxia-inducible factors, HIF-1 and HIF-2. Overexpression of HIF has been confirmed in solid tumors and was unfortunately accompanied with chemo- and radioresistance observed in many patients. Multiple cellular pathways resulting in HIF activation could be successfully inhibited by use of different kinds of drugs (e.g. topotecan, heat shock protein 90 and mTOR inhibitors, YC-1, pleurotin or 2-methoxyestradiol), which are being subjected into intensive investigation in clinical trials.
Jacek Wilczynski; Markus Duechler; Malgorzata Czyz. Targeting NF-κB and HIF-1 Pathways for the Treatment of Cancer: Part II. Archivum Immunologiae et Therapiae Experimentalis 2011, 59, 301 -307.
AMA StyleJacek Wilczynski, Markus Duechler, Malgorzata Czyz. Targeting NF-κB and HIF-1 Pathways for the Treatment of Cancer: Part II. Archivum Immunologiae et Therapiae Experimentalis. 2011; 59 (4):301-307.
Chicago/Turabian StyleJacek Wilczynski; Markus Duechler; Malgorzata Czyz. 2011. "Targeting NF-κB and HIF-1 Pathways for the Treatment of Cancer: Part II." Archivum Immunologiae et Therapiae Experimentalis 59, no. 4: 301-307.
There are still controversies concerning the role of immunological mechanisms engaged both in recurrent abortions (RA) and pre-eclampsia (PE). According to some opinions, recurrent miscarriage is comparable to organ-specific autoimmune disease. Analysis of immune reactions shows that graft rejection shares many similar mechanisms with RA and PE. This fact allows us to conclude that rejection of transplanted alloantigenic organs and pregnancy loss have probably the same evolutionary origin. Subsets and functions of immunocompetent cells (T CD4, suppressor γδT, cytotoxic T CD8, Treg, Tr1, uterine NK cells), over-activation of innate immunity (activation of NK cytotoxic cells, macrophages, neutrophils and complement), changes of Th1/Th2 cytokine balance (IL-2, IL-12, IL-15, IL-18, IFNγ, TNFα vs. IL-4, IL-10, TGFβ), importance of HLA-G molecule, CD200/CD200R interaction, over-expression of adhesion molecules, fgl2 prothrombinase activation and stimulation of IDO and HO expression, all suggest that RA and PE are syndromes of fetal allograft rejection, and not organ-specific autoimmune diseases. According to that supposition, an analogy might exist between acute graft rejection and recurrent abortion, and between chronic graft rejection and pre-eclampsia.
Jacek R. Wilczyński. Immunological Analogy Between Allograft Rejection, Recurrent Abortion and Pre-Eclampsia – the Same Basic Mechanism? Human Immunology 2006, 67, 492 -511.
AMA StyleJacek R. Wilczyński. Immunological Analogy Between Allograft Rejection, Recurrent Abortion and Pre-Eclampsia – the Same Basic Mechanism? Human Immunology. 2006; 67 (7):492-511.
Chicago/Turabian StyleJacek R. Wilczyński. 2006. "Immunological Analogy Between Allograft Rejection, Recurrent Abortion and Pre-Eclampsia – the Same Basic Mechanism?" Human Immunology 67, no. 7: 492-511.
Jacek R Wilczyńqski; Anna Nowińska; Marian Szpakowski; Marek Nowak; Artur Szpakowski; Jacek Władziński; Maja Kufelnicka; Wojciech Znojek. [Laparoscopic treatment of benign ovarian tumors]. Ginekologia Polska 2006, 77, 40 -7.
AMA StyleJacek R Wilczyńqski, Anna Nowińska, Marian Szpakowski, Marek Nowak, Artur Szpakowski, Jacek Władziński, Maja Kufelnicka, Wojciech Znojek. [Laparoscopic treatment of benign ovarian tumors]. Ginekologia Polska. 2006; 77 (1):40-7.
Chicago/Turabian StyleJacek R Wilczyńqski; Anna Nowińska; Marian Szpakowski; Marek Nowak; Artur Szpakowski; Jacek Władziński; Maja Kufelnicka; Wojciech Znojek. 2006. "[Laparoscopic treatment of benign ovarian tumors]." Ginekologia Polska 77, no. 1: 40-7.
For years conception of Th2 overbalance during pregnancy has been a paradigm for immunology of reproduction, while Th1 activity has been presented as unwanted component. Studies concerning Th1/Th2 balance in physiological and complicated pregnancy have been reviewed. Th1 activity during early peri-implantation period, premature and term labour not only accompanies but even predominates over Th2 activity. Th1 activity plays important role in promotion of Th2 response, regulation of placentation process, defense against infections and initiation of delivery. Together with Th2 activity it is necessary component of immunological reactions during pregnancy, both activities being inseparable like yin and yang. So paradigm of "Th1-Th2 cooperation" is much closer to reality than "Th2 phenomenon".
Jacek R. Wilczyński. Th1/Th2 cytokines balance—yin and yang of reproductive immunology. European Journal of Obstetrics & Gynecology and Reproductive Biology 2005, 122, 136 -143.
AMA StyleJacek R. Wilczyński. Th1/Th2 cytokines balance—yin and yang of reproductive immunology. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2005; 122 (2):136-143.
Chicago/Turabian StyleJacek R. Wilczyński. 2005. "Th1/Th2 cytokines balance—yin and yang of reproductive immunology." European Journal of Obstetrics & Gynecology and Reproductive Biology 122, no. 2: 136-143.
Background: Excessive Th1 activity in peripheral blood plays a probable role in the pathogenesis of preeclampsia. The aim of the study was to investigate whether disturbed local immune reactions are also present in decidua. Methods: Flow cytometric analysis of CD3, CD19, CD56/CD16, CD4, CD8, CD4/CD29, CD4/CD45RA, CD4/CD45RO, CD8/CD28, CD3/CD69 lymphocyte subsets isolated from third trimester decidua of pregnants with preeclampsia (n=21) and pregnant controls (n=11) subjected to elective caesarean sections. Spontaneous and phytohemaglutynine stimulated "in vitro" secretion of IL-2, IL-4, IL-6, IL-10, IL-12, IFN-γ and TGF-β by decidual lymphocytes was studied by ELISA. For the statistical significance of differences between the groups the U Mann–Whitney test was performed (confidence interval P Results: Preeclamptic patients were characterized with an increased percentage of the CD3−/CD56+CD16+, CD8+/CD28+ and decreased percentage of CD3+, CD19+, CD4+/CD45RA+ lymphocytes. The profile of secreted cytokines shifts in favor of Th1 activity (extremely high IFN-γ and low IL-6 and IL-10 secretion). Decidual IL-12 secretion in preeclamptic patients is decreased compared to controls. Conclusion: Changes in NK and T lymphocyte subsets followed with Th1 cytokine IFN-γ over-activity, could affect local immunoregulatory mechanisms in third trimester decidua of preeclamptic patients.
Jacek R. Wilczyński; Henryk Tchórzewski; Małgorzata Banasik; Ewa Głowacka; Artur Wieczorek; Przemysław Lewkowicz; Andrzej Malinowski; Marian Szpakowski; Jan Wilczyński. Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia. European Journal of Obstetrics & Gynecology and Reproductive Biology 2003, 109, 8 -15.
AMA StyleJacek R. Wilczyński, Henryk Tchórzewski, Małgorzata Banasik, Ewa Głowacka, Artur Wieczorek, Przemysław Lewkowicz, Andrzej Malinowski, Marian Szpakowski, Jan Wilczyński. Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2003; 109 (1):8-15.
Chicago/Turabian StyleJacek R. Wilczyński; Henryk Tchórzewski; Małgorzata Banasik; Ewa Głowacka; Artur Wieczorek; Przemysław Lewkowicz; Andrzej Malinowski; Marian Szpakowski; Jan Wilczyński. 2003. "Lymphocyte subset distribution and cytokine secretion in third trimester decidua in normal pregnancy and preeclampsia." European Journal of Obstetrics & Gynecology and Reproductive Biology 109, no. 1: 8-15.
If overexpression of intercellular adhesion molecule-1 (CD54) on lymphocytes exists it could have important implications for the pathophysiology of pregnancy-induced hypertension (PIH). CD54 "in vitro" expression (described as (%) of CD54+cells and CD54 mean fluorescence intensity, MFI) on the peripheral blood and decidual lymphocytes of pregnant with pre-eclampsia (PE) (n=16), transient hypertension (TH) (n=12), and controls (n=9). The percent (%) of CD54+peripheral blood lymphocytes, CD54 MFI on them and CD54 MFI on decidual lymphocytes were increased especially in PE. During PE the (%) of CD54 decidual lymphocytes correlated negatively with platelet count. In TH the positive correlation between the blood pressure and the (%) of CD54 peripheral blood lymphocytes as well as CD54 MFI on decidual lymphocytes were found. (1) PIH, especially PE, is accompanied by overexpression of intercellular adhesion molecule-1 (ICAM-1) on peripheral blood and decidual lymphocytes studied "in vitro", (2) Some of the parameters studied seem to correlate with clinical markers of PIH intensity but this fact needs further investigations using larger subject groups.
Jacek R. Wilczyński; Małgorzata Banasik; Henryk Tchórzewski; Ewa Głowacka; Andrzej Malinowski; Marian Szpakowski; Przemysław Lewkowicz; Artur Wieczorek; Krzysztof Zeman; Jan Wilczyński. Expression of intercellular adhesion molecule-1 on the surface of peripheral blood and decidual lymphocytes of women with pregnancy-induced hypertension. European Journal of Obstetrics & Gynecology and Reproductive Biology 2002, 102, 15 -20.
AMA StyleJacek R. Wilczyński, Małgorzata Banasik, Henryk Tchórzewski, Ewa Głowacka, Andrzej Malinowski, Marian Szpakowski, Przemysław Lewkowicz, Artur Wieczorek, Krzysztof Zeman, Jan Wilczyński. Expression of intercellular adhesion molecule-1 on the surface of peripheral blood and decidual lymphocytes of women with pregnancy-induced hypertension. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2002; 102 (1):15-20.
Chicago/Turabian StyleJacek R. Wilczyński; Małgorzata Banasik; Henryk Tchórzewski; Ewa Głowacka; Andrzej Malinowski; Marian Szpakowski; Przemysław Lewkowicz; Artur Wieczorek; Krzysztof Zeman; Jan Wilczyński. 2002. "Expression of intercellular adhesion molecule-1 on the surface of peripheral blood and decidual lymphocytes of women with pregnancy-induced hypertension." European Journal of Obstetrics & Gynecology and Reproductive Biology 102, no. 1: 15-20.
BACKGROUND: Transient hypertension (TH) and preeclampsia (PE) are believed to have different pathophysiology. However, 15-25% of pregnant women initially diagnosed as having TH develop PE. To clarify the immuno-pathogenetical connections between the two syndromes, we studied the pattern of T helper cell (Th)1/Th2 cytokine balance disturbances existing inside maternal decidua in normal pregnancy (NP) and pregnancies complicated with TH and PE. METHODS: Third-trimester decidual tissue was obtained by curettage of uterine cavity during elective caesarean sections in NP (n = 11), TH (n = 17) and PE (n = 21) patients. Cell suspensions were prepared by an electromechanical dispersal method and centrifugated using a standard gradient sedimentation technique. Isolated lymphocytes were placed in medium (RPMI 1640, 10% fetal calf serum, L-glutamine, penicillin, streptomycin) and cultured for 72 h with or without mitogen phytohaemaglutinine (PHA). The enzyme-linked immunosorbent assay method was used for estimation of interleukin (IL)-2, IL-4, IL-6, IL-10, IL-12 and interferon-gamma (IFN-gamma) in culture supernatant. STATISTICAL ANALYSIS: The Kruskal-Wallis and the Mann-Whitney U tests were used (p < 0.05). RESULTS: Both spontaneous and PHA-stimulated secretion of Th2-type cytokines IL-6 and IL-10 was decreased in PE patients compared with TH and NP patients. The concentration of Th1-type cytokine IFN-gamma was increased in patients suffering both from TH and PE. CONCLUSION: On the base of decidual cytokine secretion, both PE and TH are syndromes of local Th1/Th2 cytokine balance disturbances as compared with NP, and TH seems to be an intermediate step to PE.
Jacek R. Wilczyński; Henryk Tchórzewski; Ewa Głowacka; Małgorzata Banasik; Przemyslaw Lewkowicz; Marian Szpakowski; Krzysztof Zeman; Jan Wilczyński. Cytokine secretion by decidual lymphocytes in transient hypertension of pregnancy and pre-eclampsia. Mediators of Inflammation 2002, 11, 105 -111.
AMA StyleJacek R. Wilczyński, Henryk Tchórzewski, Ewa Głowacka, Małgorzata Banasik, Przemyslaw Lewkowicz, Marian Szpakowski, Krzysztof Zeman, Jan Wilczyński. Cytokine secretion by decidual lymphocytes in transient hypertension of pregnancy and pre-eclampsia. Mediators of Inflammation. 2002; 11 (2):105-111.
Chicago/Turabian StyleJacek R. Wilczyński; Henryk Tchórzewski; Ewa Głowacka; Małgorzata Banasik; Przemyslaw Lewkowicz; Marian Szpakowski; Krzysztof Zeman; Jan Wilczyński. 2002. "Cytokine secretion by decidual lymphocytes in transient hypertension of pregnancy and pre-eclampsia." Mediators of Inflammation 11, no. 2: 105-111.