Background. The occurrence and complications of diabetes are increasing worldwide. This study examined the prevalence and determinants of diabetic foot ulcers and lower extremity amputations in three selected tertiary hospitals in Ghana. Methods. A cross-sectional multicenter study involving 100 subjects was carried out. Subjects were selected through simple random sampling from three selected tertiary hospitals in Ghana. A structured questionnaire was used to document information on sociodemographic, medical history, lifestyle, and physical characteristics of subjects. Foot ulcers and lower extremity amputations were also investigated. Total cholesterol, triglycerides, low-density lipoproteins, high-density lipoproteins, serum urea, serum creatinine, and estimated glomerular filtration rate of subjects were assessed. Data analysis was done using SPSS version 22. Results. The study revealed that 31% and 69% were males and females, respectively, with a mean age of 53.8±13.8 years. Among the patients, 11% had diabetic foot ulcers whilst 3% had lower extremity amputations. In the multivariate binary logistic regression analysis, previous history of foot ulcers (OR=40.4, 95% CI=5.5-299.9) and foot deformities (OR=14.4, 95% CI=1.3-161.2) were identified as independent predictors of diabetic foot ulcers. Foot deformity (p=0.043) and serum urea (p=0.002) were associated with diabetic lower extremity amputations in the univariate analysis. Conclusion. This study showed that the prevalences of diabetic foot ulcers and lower extremity amputations are high among diabetes patients. Foot deformities and previous history of foot ulcers are determinants of diabetic foot ulcers. Foot deformity and serum urea are associated with diabetic lower extremity amputations.

The use of plant-based medicine is popular amongst individuals and communities in developing countries. Duranta erecta has been used in Africa and Asia to treat a wide range of diseases. This study evaluated the phytochemical profile and antioxidant and antimicrobial activities of D. erecta to ascertain its health benefits in traditional medicine. Phytochemical constituents and antimicrobial effect of the hydroethanolic extract of D. erecta leaves (DRL), unripe fruits (DRU), and ripe fruits (DRR) were investigated by standard methods. Elemental analyses were carried out by atomic absorption spectroscopy (AAS) on the raw sample and extract. FTIR and UV-VIS spectroscopy were used to identify functional groups. Extracts were screened for their possible antioxidant activities by three tests. The total phenolic and total tannin contents were evaluated by using the Folin–Ciocalteu method. Total flavonoid content was determined by the aluminium chloride colorimetric assay method. The antioxidant activities were evaluated using the DPPH scavenging activity. The results of phytochemical screening showed the presence of triterpenoids, sterols, alkaloids, flavonoids, saponins, glycosides, and tannins. FTIR analysis revealed the presence of alcohols, phenols, alkanes, aldehydes, ketones, aromatics, aliphatic amines, aromatic amines, amides, carboxylic acids, esters, nitro compounds, alkynes, primary and secondary amines, and alkyl halides. Iron, zinc, and copper were also detected. Total phenolic and tannin contents ranged from 2.20 ± 0.15 to 14.54 ± 0.29 mg gallic acid equivalent (GAE)/100 g and 3.55 ± 0.07 to 13.82 ± 0.04 mg GAE/100 g, respectively. Total flavonoid content varied from 41.76 ± 0.96 to 343.49 ± 3.45 μg quercetin equivalent (QE)/100 g. The highest DPPH scavenging activity was recorded in the methanolic fraction of the leaves. The antimicrobial assay of the extract or fractions recorded no activity against the test organisms. The outcome of this study affirmed that D. erecta contains phytochemicals and bioactive compounds that could be of health benefit.

Pica, an eating disorder in which non-nutritional objects are frequently eaten, has negative health implications. Despite this, pica is less studied in many African communities where it is believed to be highly prevalent. This study therefore sought to determine the prevalence of pica and its various forms among pregnant women in Kumasi, Ghana, and the effects of education and place of residence (rural and urban) on pica practice. A random sample of pregnant women (n = 400) in rural and urban areas of Kumasi were interviewed using a questionnaire-based survey in 2008 and repeated in 2009. The results showed 47.0% of the pregnant women practising pica. Pagophagia accounted for 41.0%, followed by geophagia (29.8%), amylophagia (7.4%), plumbophagia (6.4%), and trichophagia (3.7%). Among the rural dwellers, 47.7% of the pregnant women practised pica during their pregnancies while 46.4% of the urban pregnant women engaged in the practice. Age and level of education did not significantly affect the practice of pica (P = 0.053 and P = 0.142 respectively). Also, 17.4% of the respondents identified a family member practising pica. Pica is therefore highly prevalent in pregnant women in Kumasi, with pagophagia and geophagia being the predominant types of pica.

Tecoma stans (yellow bell) is a popular ornamental plant mostly found in the tropical regions. It is reported to have significant pharmacological activity and has gained attention by natives of various cultures. This study focused on the phytoconstituents screening, antioxidant activity, and heavy metal analysis as well as the acute and subchronic toxicity of the hydroethanolic leaf extract (TSE) using standard methods. The presence of flavonoids, alkaloids, cardiac glycosides, saponins, coumarins, and terpenoids in the raw leaf of the plant was observed while the hydroethanolic extract contained coumarins, saponins, cardiac glycosides, and flavonoids. The DPPH percentage scavenging activity of the crude extract was 64.32% while the fractions, ethyl acetate (55.26%), methanol (60.72%), and hydro (36.97%.), gave varying activities. The UV-Vis and FT-IR indicated the presence of alcohols, phenols, alkanes, alkenes, carbonyls (general), aliphatic amines, aromatics, ketones, ethers, esters, carboxylic acids, alkyl halides, saturated aliphatic acids, 1° and 2° amines, amides, and α,β-unsaturated aldehydes. The heavy metal analysis showed a high level of iron (Fe) and zinc (Zn) in the raw leaf. The median acute toxicity (LD₅₀) of the extract was determined to be <5000mg/kg body weight in mice. Subchronic use for 28 days resulted in significant weight gain, reduction in platelet levels, decrease in WBCs, and increase in blood glucose compared to the normal. TSE caused no adverse effects on vital organs. No mortality was recorded. The hydroethanolic extract of T. stans could therefore be considered safe in moderate doses.

Liver diseases have now become a global canker due to increasing drug abuse and several viral infections. The current medicines on the market are woefully inadequate and limited in the application against these diseases. Fortunately, medicinal plants continue to serve as a potential source of drug discovery that could be explored to improve the situation. The present study, therefore, evaluated the hepatoprotective activities of the aqueous extract of various parts (leaves, flower and stem) of Ocimum americanum L on carbon tetrachloride (CCl₄)- and acetaminophen-induced toxicity in rats. The protective effect of the plant was assessed using biochemical parameters, histology, levels of liver antioxidants, and expression of some pro-inflammatory cytokines (NF-κβ and IL-1) in the liver. The leaves and stem extracts, orally administered for 7 days at 250 mg/kg, effectively prevented CCl₄-induced elevation of serum biochemical parameters, prooxidants, as well as the expression of NFk-B and IL-1, which were comparable to Silymarin (standard drug). A comparative histopathological analyses of the liver exhibited virtually normal architecture compared with CCl₄-treated group. The findings showed that the hepatoprotective effect of Ocimum americanum was probably due to the inhibition of oxidative stress and downregulation of proinflammatory cytokines by the effective parts of the medicinal plant.