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Fei Tian; Sang Yoo Lee; So Young Woo; Hwa Young Choi; Seongeun Heo; Gyoungju Nah; Hyang Sook Chun. Author Correction: Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production. Scientific Reports 2021, 11, 1 -1.
AMA StyleFei Tian, Sang Yoo Lee, So Young Woo, Hwa Young Choi, Seongeun Heo, Gyoungju Nah, Hyang Sook Chun. Author Correction: Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production. Scientific Reports. 2021; 11 (1):1-1.
Chicago/Turabian StyleFei Tian; Sang Yoo Lee; So Young Woo; Hwa Young Choi; Seongeun Heo; Gyoungju Nah; Hyang Sook Chun. 2021. "Author Correction: Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production." Scientific Reports 11, no. 1: 1-1.
This study aimed to discriminate between the geographical origins of Asian red pepper powders distributed in Korea using Fourier-transform infrared (FT-IR) spectroscopy coupled with multivariate statistical analyses. Second-derivative spectral data were obtained from a total of 105 red pepper powder samples, 86 of which were used for statistical analysis, and the remaining 19 were used for blind testing. A one-way analysis of variance (ANOVA) test confirmed that eight peak variables exhibited significant origin-dependent differences, and the canonical discriminant functions derived from these variables were used to correctly classify all the red pepper powder samples based on their origins. The applicability of the canonical discriminant functions was examined by performing a blind test wherein the origins of 19 new red pepper powder samples were correctly classified. For simplicity, the four most significant variables were selected as discriminant indicator variables, and the applicable range for each indicator variable was set for each geographical origin. By applying the indicator variable ranges, the origins of the red pepper powders of all the statistical and blind samples were correctly identified. The study findings indicate the feasibility of using FT-IR spectroscopy in combination with multivariate analysis for identifying the geographical origins of red pepper powders.
Miso Kim; Junyoung Hong; Dongwon Lee; Sohyun Kim; Hyang Chun; Yoon-Ho Cho; Byung Kim; Sangdoo Ahn. Discriminant Analysis of the Geographical Origin of Asian Red Pepper Powders Using Second-Derivative FT-IR Spectroscopy. Foods 2021, 10, 1034 .
AMA StyleMiso Kim, Junyoung Hong, Dongwon Lee, Sohyun Kim, Hyang Chun, Yoon-Ho Cho, Byung Kim, Sangdoo Ahn. Discriminant Analysis of the Geographical Origin of Asian Red Pepper Powders Using Second-Derivative FT-IR Spectroscopy. Foods. 2021; 10 (5):1034.
Chicago/Turabian StyleMiso Kim; Junyoung Hong; Dongwon Lee; Sohyun Kim; Hyang Chun; Yoon-Ho Cho; Byung Kim; Sangdoo Ahn. 2021. "Discriminant Analysis of the Geographical Origin of Asian Red Pepper Powders Using Second-Derivative FT-IR Spectroscopy." Foods 10, no. 5: 1034.
Aflatoxins are a group of carcinogenic and mutagenic fungal secondary metabolites that have threatened human health and global food security. Aflatoxin contamination can be controlled by applying fungicides, such as strobilurins. Although these compounds have been effective, they may be risky to the environment due to their wide usage. In this study, plant-based compounds were tested to promote the performance of strobilurins (azoxystrobin, pyraclostrobin) against aflatoxigenic Aspergillus flavus; six natural compounds, namely baicalein, nobiletin, meso-dihydroguaiaretic acid, pinoresinol, syringaresinol, and celastrol, were found to exhibit synergistic antifungal effects with strobilurins with fractional inhibitory concentration index < 0.5. Among them, baicalein showed no inhibitory effects on A. flavus when applied alone, but strongly enhanced the in vitro and in situ antifungal and antiaflatoxigenic efficacy of strobilurins and transformed them from fungistatic to fungicidal agents. Therefore, baicalein may be used as an effective natural chemosensitizing agent to improve the performance of strobilurins against A. flavus. The findings of this study provide novel insights for the development of safer and more effective strategies for the control of aflatoxin contamination.
Fei Tian; Sang Yoo Lee; So Young Woo; Hwa Young Choi; Su Been Park; Hyang Sook Chun. Effect of plant-based compounds on the antifungal and antiaflatoxigenic efficiency of strobilurins against Aspergillus flavus. Journal of Hazardous Materials 2021, 415, 125663 .
AMA StyleFei Tian, Sang Yoo Lee, So Young Woo, Hwa Young Choi, Su Been Park, Hyang Sook Chun. Effect of plant-based compounds on the antifungal and antiaflatoxigenic efficiency of strobilurins against Aspergillus flavus. Journal of Hazardous Materials. 2021; 415 ():125663.
Chicago/Turabian StyleFei Tian; Sang Yoo Lee; So Young Woo; Hwa Young Choi; Su Been Park; Hyang Sook Chun. 2021. "Effect of plant-based compounds on the antifungal and antiaflatoxigenic efficiency of strobilurins against Aspergillus flavus." Journal of Hazardous Materials 415, no. : 125663.
Aflatoxin is a group of polyketide-derived carcinogenic and mutagenic secondary metabolites produced by Aspergillus flavus that negatively impact global food security and threaten the health of both humans and livestock. Aflatoxin biosynthesis is strongly affected by the fungal developmental stage, cultivation conditions, and environmental stress. In this study, a novel float culture method was used to examine the direct responses of the A. flavus transcriptome to temperature stress, oxidative stress, and their dual effects during the aflatoxin production stage. The transcriptomic response of A. flavus illustrated that the co-regulation of different secondary metabolic pathways likely contributes to maintaining cellular homeostasis and promoting cell survival under stress conditions. In particular, aflatoxin biosynthetic gene expression was downregulated, while genes encoding secondary metabolites with antioxidant properties, such as kojic acid and imizoquins, were upregulated under stress conditions. Multiple mitochondrial function-related genes, including those encoding NADH:ubiquinone oxidoreductase, ubiquinol-cytochrome C reductase, and alternative oxidase, were differentially expressed. These data can provide insights into the important mechanisms through which secondary metabolism in A. flavus is co-regulated and facilitate the deployment of various approaches for the effective control and prevention of aflatoxin contamination in food crops.
Fei Tian; Sang Yoo Lee; So Young Woo; Hwa Young Choi; Seongeun Heo; Gyoungju Nah; Hyang Sook Chun. Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production. Scientific Reports 2021, 11, 1 -12.
AMA StyleFei Tian, Sang Yoo Lee, So Young Woo, Hwa Young Choi, Seongeun Heo, Gyoungju Nah, Hyang Sook Chun. Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production. Scientific Reports. 2021; 11 (1):1-12.
Chicago/Turabian StyleFei Tian; Sang Yoo Lee; So Young Woo; Hwa Young Choi; Seongeun Heo; Gyoungju Nah; Hyang Sook Chun. 2021. "Transcriptomic responses of Aspergillus flavus to temperature and oxidative stresses during aflatoxin production." Scientific Reports 11, no. 1: 1-12.
Fungal metabolism is affected by both the developmental stage and cultivation conditions. Fungal growth in solid culture reflects natural conditions more closely than growth in liquid culture; however, because the mycelium cannot be harvested easily and the medium composition cannot be modified during incubation, the approach has some limitations when compared to liquid culture methods. The float culture incubation method introduced herein enables fungus to develop similar colonies to those on solid culture. This is a simple method that leads to the production of high-quality RNA samples.
Fei Tian; Sang Yoo Lee; So Young Woo; Hwa Young Choi; Hyang Sook Chun. A float culture method for fungal secondary metabolism study using hydrophilic polyvinylidene fluoride membranes. Analytical Biochemistry 2020, 599, 113722 .
AMA StyleFei Tian, Sang Yoo Lee, So Young Woo, Hwa Young Choi, Hyang Sook Chun. A float culture method for fungal secondary metabolism study using hydrophilic polyvinylidene fluoride membranes. Analytical Biochemistry. 2020; 599 ():113722.
Chicago/Turabian StyleFei Tian; Sang Yoo Lee; So Young Woo; Hwa Young Choi; Hyang Sook Chun. 2020. "A float culture method for fungal secondary metabolism study using hydrophilic polyvinylidene fluoride membranes." Analytical Biochemistry 599, no. : 113722.
Aflatoxins are among the most hazardous natural cereal contaminants. These mycotoxins are produced by Aspergillus spp. as polyketide secondary metabolites. Aflatoxigenic fungi including A. flavus express the alternative oxidase (AOX), which introduces a branch in the cytochrome-based electron transfer chain by coupling ubiquinol oxidation directly with the reduction of O2 to H2O. AOX is closely associated with fungal pathogenesis, morphogenesis, stress signaling, and drug resistance and, as recently reported, affects the production of mycotoxins such as sterigmatocystin, the penultimate intermediate in aflatoxin B1 biosynthesis. Thus, AOX might be considered a target for controlling the propagation of and aflatoxin contamination by A. flavus. Hence, this review summarizes the current understanding of fungal AOX and the alternative respiration pathway and the development and potential applications of AOX inhibitors. This review indicates that AOX inhibitors, either alone or in combination with current antifungal agents, are potentially applicable for developing novel, effective antifungal strategies. However, considering the conservation of AOX in fungal and plant cells, a deeper understanding of fungal alternative respiration and fungal AOX structure is needed, along with effective fungal-specific AOX inhibitors.
Fei Tian; Sang Yoo Lee; So Young Woo; Hyang Sook Chun. Alternative Oxidase: A Potential Target for Controlling Aflatoxin Contamination and Propagation of Aspergillus flavus. Frontiers in Microbiology 2020, 11, 419 .
AMA StyleFei Tian, Sang Yoo Lee, So Young Woo, Hyang Sook Chun. Alternative Oxidase: A Potential Target for Controlling Aflatoxin Contamination and Propagation of Aspergillus flavus. Frontiers in Microbiology. 2020; 11 ():419.
Chicago/Turabian StyleFei Tian; Sang Yoo Lee; So Young Woo; Hyang Sook Chun. 2020. "Alternative Oxidase: A Potential Target for Controlling Aflatoxin Contamination and Propagation of Aspergillus flavus." Frontiers in Microbiology 11, no. : 419.
Major type B trichothecene mycotoxins, including deoxynivalenol (DON), nivalenol (NIV), and their respective glucoside conjugates, deoxynivalenol-3-β-D-glucose (DON3G) and nivalenol-3-β-D-glucose (NIV3G), are present in food products, such as cereals, legumes, and their processed products. Thus, here, DON, NIV, and their 3-β-D-glucosides were monitored in 506 Korean market foods, and exposure to these mycotoxins was estimated in the population consuming these foods. The accuracy and precision of our method, which simultaneously determined four toxins, were 80.1–106.5% and 0.3–12.4%, in four representative food matrices assessed. The incidences of DON, DON3G, NIV, and NIV3G among all food samples tested were 13%, 8%, 12%, and 5%, respectively. The glucoside conjugate with free toxin was found to have the maximum co-occurrence of 49%. The estimated daily intakes of DON, DON3G, NIV, and NIV3G through food intake under four different scenarios were 0.019–0.102, 0.004–0.089, 0.007–0.094, and 0.002–0.095 μg kg−1 body weight (b.w.) day−1, respectively, which are lower than the established health-based guidance values. Overall, our results suggest that the estimated exposure of the Korean population to type B trichothecenes, namely, DON, NIV, and their 3-β-D-glucoside conjugates, may not pose a potential health risk.
Sang Yoo Lee; So Young Woo; Fei Tian; Jeonghun Song; Herbert Michlmayr; Jung-Bok Kim; Hyang Sook Chun. Occurrence of Deoxynivalenol, Nivalenol, and Their Glucosides in Korean Market Foods and Estimation of Their Population Exposure through Food Consumption. Toxins 2020, 12, 89 .
AMA StyleSang Yoo Lee, So Young Woo, Fei Tian, Jeonghun Song, Herbert Michlmayr, Jung-Bok Kim, Hyang Sook Chun. Occurrence of Deoxynivalenol, Nivalenol, and Their Glucosides in Korean Market Foods and Estimation of Their Population Exposure through Food Consumption. Toxins. 2020; 12 (2):89.
Chicago/Turabian StyleSang Yoo Lee; So Young Woo; Fei Tian; Jeonghun Song; Herbert Michlmayr; Jung-Bok Kim; Hyang Sook Chun. 2020. "Occurrence of Deoxynivalenol, Nivalenol, and Their Glucosides in Korean Market Foods and Estimation of Their Population Exposure through Food Consumption." Toxins 12, no. 2: 89.
The aim of this study was to discriminate between Korean and Chinese perilla seeds by using inorganic elemental analysis data combined with orthogonal partial least squares-discriminant analysis (OPLS-DA). Inorganic element content data were obtained from 29 Korean and 11 Chinese perilla seed samples distributed in Korea. Using energy dispersive X-ray fluorescence spectroscopy, 11 species of inorganic elements were detected in the perilla seed samples and eight of them were quantified. The Rb content was selected as the variable to best discriminate between Korean and Chinese perilla seeds using an S-plot generated from OPLS-DA. An accurate range for the Rb content was determined using inductively coupled plasma-optical emission spectroscopy. The Rb content is remarkably higher in Chinese perilla seeds (8.7–19.0 mg/kg) than in Korean perilla seeds (0.0–7.6 mg/kg), thereby suggesting that Rb analysis may be an appropriate approach to discriminate Chinese perilla seeds from Korean perilla seeds.
Jung Eun Lee; Jihyun Hwang; Eunji Choi; Myoung-Jae Shin; Hyang Sook Chun; Sangdoo Ahn; Byung Hee Kim. Rubidium analysis as a possible approach for discriminating between Korean and Chinese perilla seeds distributed in Korea. Food Chemistry 2019, 312, 126067 .
AMA StyleJung Eun Lee, Jihyun Hwang, Eunji Choi, Myoung-Jae Shin, Hyang Sook Chun, Sangdoo Ahn, Byung Hee Kim. Rubidium analysis as a possible approach for discriminating between Korean and Chinese perilla seeds distributed in Korea. Food Chemistry. 2019; 312 ():126067.
Chicago/Turabian StyleJung Eun Lee; Jihyun Hwang; Eunji Choi; Myoung-Jae Shin; Hyang Sook Chun; Sangdoo Ahn; Byung Hee Kim. 2019. "Rubidium analysis as a possible approach for discriminating between Korean and Chinese perilla seeds distributed in Korea." Food Chemistry 312, no. : 126067.
The antifungal and antiaflatoxigenic activity of Thymus vulgaris essential oil (EO) against Aspergillus flavus was evaluated over a range of concentrations in vapor- and liquid-phase contact tests. Total reduction in mycelial growth in the vapor- and liquid-phase tests was detected at EO concentrations of 20 and 400 μg/mL, respectively. Treatment with 10 μg/mL EO reduced aflatoxin production by 97.0 and 56.4% in the vapor- and liquid-phase tests, respectively. Greater inhibition of the expression of both fungal development–related genes (brlA, abaA, and wetA) and aflatoxin biosynthesis–related genes (aflR, aflD, and aflK) was also observed in the vapor-phase test. A substantial reduction in aflatoxin production was also observed in brown rice (72.7%) and white rice (18.0%). Our results indicate that the way this EO contacts fungal cells significantly affects its antifungal activity and that T. vulgaris EO in vapor phase might be a good strategy to control fungal contamination. HIGHLIGHTS
Fei Tian; Sang Yoo Lee; Hyang Sook Chun. Comparison of the Antifungal and Antiaflatoxigenic Potential of Liquid and Vapor Phase of Thymus vulgaris Essential Oil Against Aspergillus flavus. Journal of Food Protection 2019, 82, 2044 -2048.
AMA StyleFei Tian, Sang Yoo Lee, Hyang Sook Chun. Comparison of the Antifungal and Antiaflatoxigenic Potential of Liquid and Vapor Phase of Thymus vulgaris Essential Oil Against Aspergillus flavus. Journal of Food Protection. 2019; 82 (12):2044-2048.
Chicago/Turabian StyleFei Tian; Sang Yoo Lee; Hyang Sook Chun. 2019. "Comparison of the Antifungal and Antiaflatoxigenic Potential of Liquid and Vapor Phase of Thymus vulgaris Essential Oil Against Aspergillus flavus." Journal of Food Protection 82, no. 12: 2044-2048.
The authors wish to make the following erratum to this paper
Seo Yeong Oh; Min Ji Lee; Nam Su Heo; Suji Kim; Jeong Su Oh; Yuseon Lee; Eun Jeong Jeon; Tae Jung Park; Hyang Sook Chun; Yun Suk Huh. Erratum: Cuvette-Type LSPR Sensor for Highly Sensitive Detection of Melamine in Infant Formulas. Sensors 2018, 19(18), 3839. Sensors 2019, 19, 4630 .
AMA StyleSeo Yeong Oh, Min Ji Lee, Nam Su Heo, Suji Kim, Jeong Su Oh, Yuseon Lee, Eun Jeong Jeon, Tae Jung Park, Hyang Sook Chun, Yun Suk Huh. Erratum: Cuvette-Type LSPR Sensor for Highly Sensitive Detection of Melamine in Infant Formulas. Sensors 2018, 19(18), 3839. Sensors. 2019; 19 (21):4630.
Chicago/Turabian StyleSeo Yeong Oh; Min Ji Lee; Nam Su Heo; Suji Kim; Jeong Su Oh; Yuseon Lee; Eun Jeong Jeon; Tae Jung Park; Hyang Sook Chun; Yun Suk Huh. 2019. "Erratum: Cuvette-Type LSPR Sensor for Highly Sensitive Detection of Melamine in Infant Formulas. Sensors 2018, 19(18), 3839." Sensors 19, no. 21: 4630.
Doenjang, a Korean fermented soybean paste, is vulnerable to contamination by mycotoxins because it is directly exposed to environmental microbiota during fermentation. A method that simultaneously determines 20 mycotoxins in doenjang, including aflatoxins (AFs), ochratoxin A (OTA), zearalenone (ZEN), and fumonisins (FBs) with an immunoaffinity column cleanup was optimized and validated in doenjang using LC-MS/MS. The method showed good performance in the analysis of 20 mycotoxins in doenjang with good linearity (R2 > 0.999), intra- and inter-day precision (
So Young Woo; So Young Ryu; Fei Tian; Sang Yoo Lee; Su Been Park; Hyang Sook Chun. Simultaneous Determination of Twenty Mycotoxins in the Korean Soybean Paste Doenjang by LC-MS/MS with Immunoaffinity Cleanup. Toxins 2019, 11, 594 .
AMA StyleSo Young Woo, So Young Ryu, Fei Tian, Sang Yoo Lee, Su Been Park, Hyang Sook Chun. Simultaneous Determination of Twenty Mycotoxins in the Korean Soybean Paste Doenjang by LC-MS/MS with Immunoaffinity Cleanup. Toxins. 2019; 11 (10):594.
Chicago/Turabian StyleSo Young Woo; So Young Ryu; Fei Tian; Sang Yoo Lee; Su Been Park; Hyang Sook Chun. 2019. "Simultaneous Determination of Twenty Mycotoxins in the Korean Soybean Paste Doenjang by LC-MS/MS with Immunoaffinity Cleanup." Toxins 11, no. 10: 594.
The globalization of food distribution has made necessary to secure safe products to the general consumers through the rapid detection of harmful additives on the field. For this purpose, we developed a cuvette-type localized surface plasmon resonance (LSPR) sensor that can be easily used by consumers with conventional ultraviolet-visible light spectrophotometer for in-situ measurements. Gold nanoparticles were uniformly deposited on a transparent substrate via a self-assembly method to obtain a plasmonically active chip, and the chemical receptor p-nitroaniline (p-NA) was functionalized to stabilize the device sensitivity under external temperature and pH conditions. The fabricated chip was fixed onto a support and combined with a cuvette-type LSPR sensor. To evaluate the applicability of this sensor on the field, sensitivity and quantitative analysis experiments were conducted onto melamine as a model sample from harmful food additives. Under optimal reaction condition (2 mM p-NA for 20 min), we achieved an excellent detection limit (0.01 ppb) and a dynamic range allowing quantitative analysis over a wide concentration range (0.1–1000 ppb) from commercially available milk powder samples.
Seo Yeong Oh; Min Ji Lee; Nam Su Heo; Suji Kim; Jeong Su Oh; Yuseon Lee; Eun Jeong Jeon; Hyungsil Moon; Hyung Soo Kim; Tae Jung Park; Guiim Moon; Hyang Sook Chun; Yun Suk Huh. Cuvette-Type LSPR Sensor for Highly Sensitive Detection of Melamine in Infant Formulas. Sensors 2019, 19, 3839 .
AMA StyleSeo Yeong Oh, Min Ji Lee, Nam Su Heo, Suji Kim, Jeong Su Oh, Yuseon Lee, Eun Jeong Jeon, Hyungsil Moon, Hyung Soo Kim, Tae Jung Park, Guiim Moon, Hyang Sook Chun, Yun Suk Huh. Cuvette-Type LSPR Sensor for Highly Sensitive Detection of Melamine in Infant Formulas. Sensors. 2019; 19 (18):3839.
Chicago/Turabian StyleSeo Yeong Oh; Min Ji Lee; Nam Su Heo; Suji Kim; Jeong Su Oh; Yuseon Lee; Eun Jeong Jeon; Hyungsil Moon; Hyung Soo Kim; Tae Jung Park; Guiim Moon; Hyang Sook Chun; Yun Suk Huh. 2019. "Cuvette-Type LSPR Sensor for Highly Sensitive Detection of Melamine in Infant Formulas." Sensors 19, no. 18: 3839.
Yongsung Joo; Hyun Ee Ok; Jihyun Kim; Sang Yoo Lee; Su Kyung Jang; Ki Hwan Park; Hyang Sook Chun. A statistical model for determining zearalenone contamination in rice (Oryza sativa L.) at harvest and its prediction under different climate change scenarios in South Korea. Applied Biological Chemistry 2019, 62, 1 .
AMA StyleYongsung Joo, Hyun Ee Ok, Jihyun Kim, Sang Yoo Lee, Su Kyung Jang, Ki Hwan Park, Hyang Sook Chun. A statistical model for determining zearalenone contamination in rice (Oryza sativa L.) at harvest and its prediction under different climate change scenarios in South Korea. Applied Biological Chemistry. 2019; 62 (1):1.
Chicago/Turabian StyleYongsung Joo; Hyun Ee Ok; Jihyun Kim; Sang Yoo Lee; Su Kyung Jang; Ki Hwan Park; Hyang Sook Chun. 2019. "A statistical model for determining zearalenone contamination in rice (Oryza sativa L.) at harvest and its prediction under different climate change scenarios in South Korea." Applied Biological Chemistry 62, no. 1: 1.
The cytotoxicity of TiO2 nanoparticles are well-known, but the particle size-dependent induction of ER stress and apoptosis by TiO2 in hepatocytes has not been elucidated clearly. In the present study, we investigated whether a fine TiO2 particle and two types of TiO2 nanoparticles induce ER stress and apoptosis differently in HepG2 cells. A particle size-dependent decrease in cell viability was observed after exposure to the TiO2 particles. The levels of ER stress-related proteins (BiP, CHOP, ATF6α, and p-PERK) were increased with decreasing particle size. TiO2 particles induced ER stress-mediated apoptosis in a particle size-dependent manner as seen by a decrease in the expression of Bcl-2, and increases in the expression of Bax, caspase-12, and cleaved caspase-3. These results indicated that the cytotoxicity produced by TiO2 particles was related to particle size, with smaller TiO2 nanoparticles producing greater toxic effects involving ER stress and apoptosis in the HepG2 cells.
Ha Na Song; Su Kyung Jang; Ok Kyung Hwang; Hong Jin Lee; Hyang Sook Chun. TiO2 particles induce ER stress and apoptosis in human hepatoma cells, HepG2, in a particle size-dependent manner. Food Science and Biotechnology 2019, 28, 1907 -1917.
AMA StyleHa Na Song, Su Kyung Jang, Ok Kyung Hwang, Hong Jin Lee, Hyang Sook Chun. TiO2 particles induce ER stress and apoptosis in human hepatoma cells, HepG2, in a particle size-dependent manner. Food Science and Biotechnology. 2019; 28 (6):1907-1917.
Chicago/Turabian StyleHa Na Song; Su Kyung Jang; Ok Kyung Hwang; Hong Jin Lee; Hyang Sook Chun. 2019. "TiO2 particles induce ER stress and apoptosis in human hepatoma cells, HepG2, in a particle size-dependent manner." Food Science and Biotechnology 28, no. 6: 1907-1917.
A simple and reliable method for the simultaneous determination of major type B trichothecene mycotoxins, deoxynivalenol (DON) and nivalenol (NIV), along with their 3-β-d-glucosides (DON-3-glucoside (DON3G) and NIV-3-glucoside (NIV3G)) in baby formula and Korean rice wine was validated in the present study. The method was based on immunoaffinity cleanup followed by analysis using an HPLC-UV technique. The method was validated in-house for two matrices as follows: linearity (R2 > 0.99) was established in the range of 20–1000 μg kg–1; accuracy (expressed as recovery) ranged from 78.7 to 106.5% for all the analytes; good intermediate precision (relative standard deviation < 12%), and adequate detection and quantitation limits (< 4.4 and < 13.3 μg kg–1, respectively) were achieved. Furthermore, the estimated measurement expanded uncertainty was determined to be 4–24%. The validated method was successfully applied to the analysis of 31 baby formulas and Korean rice wines marketed in Korea.
Sang Yoo Lee; So Young Woo; Alexandra Malachová; Herbert Michlmayr; Sheen-Hee Kim; Gil Jin Kang; Hyang Sook Chun. Simple validated method for simultaneous determination of deoxynivalenol, nivalenol, and their 3-β-D-glucosides in baby formula and Korean rice wine via HPLC-UV with immunoaffinity cleanup. Food Additives & Contaminants: Part A 2019, 36, 964 -975.
AMA StyleSang Yoo Lee, So Young Woo, Alexandra Malachová, Herbert Michlmayr, Sheen-Hee Kim, Gil Jin Kang, Hyang Sook Chun. Simple validated method for simultaneous determination of deoxynivalenol, nivalenol, and their 3-β-D-glucosides in baby formula and Korean rice wine via HPLC-UV with immunoaffinity cleanup. Food Additives & Contaminants: Part A. 2019; 36 (6):964-975.
Chicago/Turabian StyleSang Yoo Lee; So Young Woo; Alexandra Malachová; Herbert Michlmayr; Sheen-Hee Kim; Gil Jin Kang; Hyang Sook Chun. 2019. "Simple validated method for simultaneous determination of deoxynivalenol, nivalenol, and their 3-β-D-glucosides in baby formula and Korean rice wine via HPLC-UV with immunoaffinity cleanup." Food Additives & Contaminants: Part A 36, no. 6: 964-975.
Perilla oils distributed in Korea were authenticated by analyzing their 13C/12C isotope ratios (expressed as δ13C) or fatty acid compositions. No significant differences were found in the δ13C values of authentic (n = 27) and adulterated (n = 10) perilla oils, whereas the distributions of 11 fatty acids were significantly different in these oils. By using orthogonal projections for latent structures discriminant analysis (OPLS‐DA) technique, the 18:2n‐6 and 18:3n‐3 content levels were determined to be the best variables for authenticating perilla oil. The authenticity of 7 of 76 blind samples were identified through δ13C values, whereas using the 18:2n‐6 and 18:3n‐3 content levels could identified the authenticity of 66 blind samples. These results suggest that fatty acid analysis is a more useful procedure for determining the perilla oil authenticity. A blind trial showed that perilla oils adulterated with corn, soybean, or perilla‐flavored oil at concentrations ≥5 vol% can be distinguished. Practical applications: Public concern about economically motivated adulteration (EMA) of food is growing worldwide. Perilla oil is a targeted product for EMA in Korea due to its high retail price. This study provides the first evidence showing that 18:2n‐6 and 18:3n‐3 content levels can become new specifications and standards for perilla oils distributed in Korea.
Jinyeong Kim; Sehee Yang; Cheon-Ho Jo; Jang-Duck Choi; Kisung Kwon; Sangdoo Ahn; Hyang Sook Chun; Byung Hee Kim. Comparison of Carbon Stable Isotope and Fatty Acid Analyses for the Authentication of Perilla Oil. European Journal of Lipid Science and Technology 2018, 120, 1 .
AMA StyleJinyeong Kim, Sehee Yang, Cheon-Ho Jo, Jang-Duck Choi, Kisung Kwon, Sangdoo Ahn, Hyang Sook Chun, Byung Hee Kim. Comparison of Carbon Stable Isotope and Fatty Acid Analyses for the Authentication of Perilla Oil. European Journal of Lipid Science and Technology. 2018; 120 (8):1.
Chicago/Turabian StyleJinyeong Kim; Sehee Yang; Cheon-Ho Jo; Jang-Duck Choi; Kisung Kwon; Sangdoo Ahn; Hyang Sook Chun; Byung Hee Kim. 2018. "Comparison of Carbon Stable Isotope and Fatty Acid Analyses for the Authentication of Perilla Oil." European Journal of Lipid Science and Technology 120, no. 8: 1.
The complete chloroplast genome sequence of Codonopsis lanceolata was determined by next generation sequencing. The total length of chloroplast genome of C. lanceolata was 169,447 bp long, including a large single-copy (LSC) region of 85,253 bp, a small single-copy (SSC) region of 8060 bp, and a pair of identical inverted repeat regions (IRs) of 38,067 bp. A total of 110 genes was annotated, resulting in 79 protein-coding genes, 27 tRNA genes, and 4 rRNA genes. The phylogenetic analysis of C. lanceolata with related chloroplast genome sequences in this study provided the taxonomical relationship of C. lanceolata in the genus Campanula.
Junki Lee; So-Yeon Kim; Jong-Sung Lim; Hyang Sook Chun; Kisung Kwon; Youngho Koh; Tae Sun Kang; Jung-Hwa Kang; Eun-Jeong Kim; Gyoungju Nah. The complete chloroplast genome of Codonopsis lanceolata (Campanulaceae). Mitochondrial DNA Part B 2018, 3, 1075 -1076.
AMA StyleJunki Lee, So-Yeon Kim, Jong-Sung Lim, Hyang Sook Chun, Kisung Kwon, Youngho Koh, Tae Sun Kang, Jung-Hwa Kang, Eun-Jeong Kim, Gyoungju Nah. The complete chloroplast genome of Codonopsis lanceolata (Campanulaceae). Mitochondrial DNA Part B. 2018; 3 (2):1075-1076.
Chicago/Turabian StyleJunki Lee; So-Yeon Kim; Jong-Sung Lim; Hyang Sook Chun; Kisung Kwon; Youngho Koh; Tae Sun Kang; Jung-Hwa Kang; Eun-Jeong Kim; Gyoungju Nah. 2018. "The complete chloroplast genome of Codonopsis lanceolata (Campanulaceae)." Mitochondrial DNA Part B 3, no. 2: 1075-1076.
The complete chloroplast genome sequence of Caltha palustris, a species of the Ranunculaceae family, was characterized from the de novo assembly of HiSeq (Illumina Co.) paired-end sequencing data. The chloroplast genome of C. palustris was 155,292 bp in length, with a large single-copy (LSC) region of 84,120 bp, a small single-copy (SSC) region of 18,342 bp, and a pair of identical inverted repeat regions (IRs) of 26,415 bp. The genome contained a total of 114 genes, including 80 protein-coding genes, 30 transfer RNA (tRNA) genes, and 4 ribosomal RNA (rRNA) genes. The phylogenetic analysis of C. palustris with 14 related species revealed the closest taxonomical relationship with Hydrastis canadensis in the Ranunculaceae family.
Junki Lee; Yoojin Kim; Hyang Sook Chun; Kisung Kwon; Youngho Koh; Tae Sun Kang; Jung-Hwa Kang; Eun-Jeong Kim; Gyoungju Nah. The complete chloroplast genome of Caltha Palustris (Ranunculaceae). Mitochondrial DNA Part B 2018, 3, 1090 -1091.
AMA StyleJunki Lee, Yoojin Kim, Hyang Sook Chun, Kisung Kwon, Youngho Koh, Tae Sun Kang, Jung-Hwa Kang, Eun-Jeong Kim, Gyoungju Nah. The complete chloroplast genome of Caltha Palustris (Ranunculaceae). Mitochondrial DNA Part B. 2018; 3 (2):1090-1091.
Chicago/Turabian StyleJunki Lee; Yoojin Kim; Hyang Sook Chun; Kisung Kwon; Youngho Koh; Tae Sun Kang; Jung-Hwa Kang; Eun-Jeong Kim; Gyoungju Nah. 2018. "The complete chloroplast genome of Caltha Palustris (Ranunculaceae)." Mitochondrial DNA Part B 3, no. 2: 1090-1091.
Three monoterpenes, 1-methyl-4-(1-methylethyl)-benzene, and its derivatives, carvacrol and thymol, were tested for their antifungal and antiaflatoxigenic activities against Aspergillus flavus, and their potential in vitro mechanisms were evaluated. The monoterpenes significantly inhibited mycelial growth, spore production, and aflatoxin production in a dose-dependent manner. Furthermore, their antifungal effects were related to the suppression of fungal development regulatory genes (brlA, abaA, and wetA) and inhibition of ergosterol synthesis. Additionally, the down-regulation of the relative expression of genes related to aflatoxin biosynthesis (aflD, aflK, aflQ, and aflR) revealed an antiaflatoxigenic mechanism of the monoterpenes. These observations suggest that the three monoterpenes exhibit antiaflatoxigenic activities through multiple modes of action and may be useful for controlling aflatoxin contamination in food.
Fei Tian; So Young Woo; Sang Yoo Lee; Hyang Sook Chun. p-Cymene and its derivatives exhibit antiaflatoxigenic activities against Aspergillus flavus through multiple modes of action. Applied Biological Chemistry 2018, 61, 489 -497.
AMA StyleFei Tian, So Young Woo, Sang Yoo Lee, Hyang Sook Chun. p-Cymene and its derivatives exhibit antiaflatoxigenic activities against Aspergillus flavus through multiple modes of action. Applied Biological Chemistry. 2018; 61 (5):489-497.
Chicago/Turabian StyleFei Tian; So Young Woo; Sang Yoo Lee; Hyang Sook Chun. 2018. "p-Cymene and its derivatives exhibit antiaflatoxigenic activities against Aspergillus flavus through multiple modes of action." Applied Biological Chemistry 61, no. 5: 489-497.
Aflatoxins are naturally occurring carcinogens. Humans and animals, such as turkeys, are highly susceptible to aflatoxin-induced diseases. In this study, we investigated the inhibitory effects of 5 food additives on the growth and aflatoxin production in Aspergillus flavus ATCC 22546. Propionic acid completely inhibited fungal growth at a concentration of 0.5%. Furthermore, no fungal growth was observed when the growth medium was treated with 0.05% benzoic acid, 0.1% sorbic acid, 0.5% acetic acid, or 0.5% butyric acid. In comparison to propionic acid, other food additives showed weak antifungal activities. Propionic acid, butyric acid, benzoic acid, and sorbic acid also exhibited potent antiaflatoxigenic activities at a concentration of 0.1%. However, addition of 0.1% acetic acid did not inhibit aflatoxin production. At a concentration of 0.05%, propionic acid lost its antiaflatoxigenic activity, whereas at the same concentration, benzoic acid, butyric acid, and sorbic acid showed potent antiaflatoxigenic activities with a 95% inhibition of aflatoxin production. Benzoic acid drastically inhibited the expression of genes related to aflatoxin biosynthesis, whereas sorbic acid only inhibited the expression of a transcription gene, yab. Collectively, our results suggest that benzoic acid is promising alternative to propionic acid as food preservatives.
Young-Sun Moon; Hyeong-Mi Kim; Hyang Sook Chun; Sung-Eun Lee. Organic acids suppress aflatoxin production via lowering expression of aflatoxin biosynthesis-related genes in Aspergillus flavus. Food Control 2018, 88, 207 -216.
AMA StyleYoung-Sun Moon, Hyeong-Mi Kim, Hyang Sook Chun, Sung-Eun Lee. Organic acids suppress aflatoxin production via lowering expression of aflatoxin biosynthesis-related genes in Aspergillus flavus. Food Control. 2018; 88 ():207-216.
Chicago/Turabian StyleYoung-Sun Moon; Hyeong-Mi Kim; Hyang Sook Chun; Sung-Eun Lee. 2018. "Organic acids suppress aflatoxin production via lowering expression of aflatoxin biosynthesis-related genes in Aspergillus flavus." Food Control 88, no. : 207-216.