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Rosa Mourão
Bionorte Post-Graduate Program (Network Program)–Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Oeste do Pará Federal University (Local Pole), Santarém 68040070, Pará, Brazil

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Communication
Published: 16 June 2021 in Molecules
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Pectis elongata is found in the northern and northeastern regions of Brazil. It is considered a lemongrass due to its citric scent. The remarkable citral content and the wide antimicrobial properties and bioactive features of this terpene make this essential oil (EO) eligible for several industrial purposes, especially in cosmetics and phytotherapics. However, to address the problems regarding citral solubility, nano-emulsification is considered a promising strategy thanks to its improved dispersability. Thus, in this paper we propose a low-energy approach for the development of citral-based nano-emulsions prepared with P. elongata EO. The plant was hydrodistillated to produce the EO, which was characterized with a gas chromatograph coupled to mass spectrometry. The nano-emulsion prepared by a non-heated water titrating (low-energy) method was composed of 5% (w/w) EO, 5% (w/w) non-ionic surfactants and 90% (w/w) deionized water and was analyzed by dynamic light scattering. Levels of citral of around 90% (neral:geranial—4:5) were detected in the EO and no major alteration in the ratio of citral was observed after the nano-emulsification. The nano-emulsion was stable until the 14th day (size around 115 nm and polydispersity index around 0.2) and no major alteration in droplet size was observed within 30 days of storage. Understanding the droplet size distribution as a function of time and correlating it to concepts of compositional ripening, as opposing forces to the conventional Ostwald ripening destabilization mechanism, may open interesting approaches for further industrial application of novel, low-energy, ecofriendly approaches to high citral essential oil-based nano-emulsions based on lemongrass plants.

ACS Style

Suelen Pereira; Adenilson Barroso; Rosa Mourão; Caio Fernandes. A Low Energy Approach for the Preparation of Nano-Emulsions with a High Citral-Content Essential Oil. Molecules 2021, 26, 3666 .

AMA Style

Suelen Pereira, Adenilson Barroso, Rosa Mourão, Caio Fernandes. A Low Energy Approach for the Preparation of Nano-Emulsions with a High Citral-Content Essential Oil. Molecules. 2021; 26 (12):3666.

Chicago/Turabian Style

Suelen Pereira; Adenilson Barroso; Rosa Mourão; Caio Fernandes. 2021. "A Low Energy Approach for the Preparation of Nano-Emulsions with a High Citral-Content Essential Oil." Molecules 26, no. 12: 3666.

Journal article
Published: 25 December 2020 in Molecules
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Aniba rosiodora has been exploited since the end of the nineteenth century for its essential oil, a valuable ingredient in the perfumery industry. This species occurs mainly in Northern South America, and the morphological similarity among different Aniba species often leads to misidentification, which impacts the consistency of products obtained from these plants. Hence, we compared the profiles of volatile organic compounds (essential oils) and non-volatile organic compounds (methanolic extracts) of two populations of A. rosiodora from the RESEX and FLONA conservation units, which are separated by the Tapajós River in Western Pará State. The phytochemical profile indicated a substantial difference between the two populations: samples from RESEX present α-phellandrene (22.8%) and linalool (39.6%) in their essential oil composition, while samples from FLONA contain mainly linalool (83.7%). The comparison between phytochemical profiles and phylogenetic data indicates a clear difference, implying genetic distinction between these populations.

ACS Style

Diana R. Amazonas; Celso Oliveira; Lauro E. S. Barata; Eric J. Tepe; Massuo J. Kato; Rosa H. V. Mourão; Lydia F. Yamaguchi. Chemical and Genotypic Variations in Aniba rosiodora from the Brazilian Amazon Forest. Molecules 2020, 26, 69 .

AMA Style

Diana R. Amazonas, Celso Oliveira, Lauro E. S. Barata, Eric J. Tepe, Massuo J. Kato, Rosa H. V. Mourão, Lydia F. Yamaguchi. Chemical and Genotypic Variations in Aniba rosiodora from the Brazilian Amazon Forest. Molecules. 2020; 26 (1):69.

Chicago/Turabian Style

Diana R. Amazonas; Celso Oliveira; Lauro E. S. Barata; Eric J. Tepe; Massuo J. Kato; Rosa H. V. Mourão; Lydia F. Yamaguchi. 2020. "Chemical and Genotypic Variations in Aniba rosiodora from the Brazilian Amazon Forest." Molecules 26, no. 1: 69.

Journal article
Published: 31 March 2020 in Revista Fitos
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Passiflora nitida Kunth. (maracujá-do-mato) é uma espécie nativa do Brasil com ocorrência nas regiões norte, nordeste e centro-oeste. O seu fruto é consumido principalmente in natura, sendo pouco explorado quanto as suas propriedades funcionais. O objetivo deste estudo, foi extrair substâncias pécticas do mesocarpo de Passiflora nitida Kunth. O mesocarpo foi desidratado, triturado e obtido um pó de fina granulação (farinha), o qual, foi caracterizado quanto a granulometria, índice de intumescência, acidez titulável, cinzas e teor de umidade. Para a extração de substâncias pécticas foi utilizado 4g da farinha, em meio ácido. Foi usado o ácido cítrico comercial e suco de limão a 5% a 60ºC e 80ºC por 60 minutos e 120 minutos. Os experimentos foram realizados em triplicata e submetidos à análise de variância. O rendimento de substâncias pécticas com suco de limão por 60 minutos a 80ºC e 120 minutos a 60ºC foi de 17,9% e 14,3%, respectivamente. Para o ácido cítrico comercial, por 60 min a 80ºC e 120 minutos a 60ºC foi de 12,9% e 12,2%, respectivamente. O mesocarpo de Passiflora nitida poderá ser uma fonte para obtenção de substâncias pécticas.

ACS Style

Juliane Tavares Gomes; Adenilson De Sousa Barroso; Rosa Helena Veras Mourão; Carlos Diego Almeida Monteiro. Extração de substâncias pécticas do mesocarpo de Passiflora nitida Kunth. Passiforaceae. Revista Fitos 2020, 14, 76 -87.

AMA Style

Juliane Tavares Gomes, Adenilson De Sousa Barroso, Rosa Helena Veras Mourão, Carlos Diego Almeida Monteiro. Extração de substâncias pécticas do mesocarpo de Passiflora nitida Kunth. Passiforaceae. Revista Fitos. 2020; 14 (1):76-87.

Chicago/Turabian Style

Juliane Tavares Gomes; Adenilson De Sousa Barroso; Rosa Helena Veras Mourão; Carlos Diego Almeida Monteiro. 2020. "Extração de substâncias pécticas do mesocarpo de Passiflora nitida Kunth. Passiforaceae." Revista Fitos 14, no. 1: 76-87.

Journal article
Published: 19 February 2020 in Biomolecules
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The essential oil of Eugenia uniflora has been attributed anti-depressive, antinociceptive, antileishmanial, larvicidal, antioxidant, antibacterial, and antifungal activities. It is known that the cultivation of this plant can be affected by seasonality, promoting alteration in the oil composition and its biological activities. This study aims to perform the annual evaluation of the curzerene-type oil of E. uniflora and determine its antioxidant activity. The oil yield from the dry season (1.4 ± 0.6%) did not differ statistically from that of the rainy season (1.8 ± 0.8%). Curzerene, an oxygenated sesquiterpene, was the principal constituent, and its percentage showed no significant difference between the two periods: dry (42.7% ± 6.1) and rainy (40.8 ± 5.9%). Principal component and hierarchical cluster analyses presented a high level of similarity between the monthly samples of the oils. Also, in the annual study, the yield and composition of the oils did not present a significant correlation with the climatic variables. The antioxidant activity of the oils showed inhibition of DPPH radicals with an average value of 55.0 ± 6.6%. The high curzerene content in the monthly oils of E. uniflora suggests their potential for use as a future phytotherapeutic alternative.

ACS Style

Jamile S. Da Costa; Adenilson S. Barroso; Rosa Helena V. Mourão; Joyce Kelly R. Da Silva; José Guilherme S. Maia; Pablo Luis B. Figueiredo. Seasonal and Antioxidant Evaluation of Essential Oil from Eugenia uniflora L., Curzerene-Rich, Thermally Produced in Situ. Biomolecules 2020, 10, 328 .

AMA Style

Jamile S. Da Costa, Adenilson S. Barroso, Rosa Helena V. Mourão, Joyce Kelly R. Da Silva, José Guilherme S. Maia, Pablo Luis B. Figueiredo. Seasonal and Antioxidant Evaluation of Essential Oil from Eugenia uniflora L., Curzerene-Rich, Thermally Produced in Situ. Biomolecules. 2020; 10 (2):328.

Chicago/Turabian Style

Jamile S. Da Costa; Adenilson S. Barroso; Rosa Helena V. Mourão; Joyce Kelly R. Da Silva; José Guilherme S. Maia; Pablo Luis B. Figueiredo. 2020. "Seasonal and Antioxidant Evaluation of Essential Oil from Eugenia uniflora L., Curzerene-Rich, Thermally Produced in Situ." Biomolecules 10, no. 2: 328.

Journal article
Published: 04 July 2019 in Revista Brasileira de Farmacognosia
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Lippia alba (Mill.) N.E.Br. ex Britton & P. Wilson, Verbenaceae, is considered a great source of a bioactive volatile oil. Due to the wide range of known chemotypes, its chemical analysis is very important. Among the several activities of this volatile oil, a potential larvicidal action against Culicidae species is highlighted. However, the low water miscibility of volatile oils limits their application in aqueous media. Oil in water nano-emulsions are in the spotlight of novelty to solve this main problem. Thus, the aim of the present study was to obtain this nanostructured system with L. alba volatile oil (citral chemotype) and evaluate its larvicidal activity against Aedes aegypti and Culex quinquefasciatus larvae. The major compounds were geranial (30.02%) and neral (25.26%). Low mean droplet size (117.0 ± 1.0 nm) and low polydispersity index (0.231 ± 0.004) were observed and no major changes were observed after seven days of storage. LC50 values against C. quinquefasciatus and A. aegypti third-instar larvae were respectively 38.22 and 31.02 ppm, while LC90 values were, respectively, 59.42 and 47.19 ppm. The present study makes use of a low energy, solvent-free and ecofriendly method with reduced costs. Thus, this paper contributes significantly to phyto-nanobiotechnology of larvicidal agents, opening perspectives for the utilization of L. alba volatile oil in integrated practices of vector control.

ACS Style

Ricardo M.A. Ferreira; Jonatas L. Duarte; Rodrigo A.S. Cruz; Anna E.M.F.M. Oliveira; Raquel Silva Araújo; Jose Carlos Tavares Carvalho; Rosa H.V. Mourão; Raimundo N.P. Souto; Caio P. Fernandes. A herbal oil in water nano-emulsion prepared through an ecofriendly approach affects two tropical disease vectors. Revista Brasileira de Farmacognosia 2019, 29, 778 -784.

AMA Style

Ricardo M.A. Ferreira, Jonatas L. Duarte, Rodrigo A.S. Cruz, Anna E.M.F.M. Oliveira, Raquel Silva Araújo, Jose Carlos Tavares Carvalho, Rosa H.V. Mourão, Raimundo N.P. Souto, Caio P. Fernandes. A herbal oil in water nano-emulsion prepared through an ecofriendly approach affects two tropical disease vectors. Revista Brasileira de Farmacognosia. 2019; 29 (6):778-784.

Chicago/Turabian Style

Ricardo M.A. Ferreira; Jonatas L. Duarte; Rodrigo A.S. Cruz; Anna E.M.F.M. Oliveira; Raquel Silva Araújo; Jose Carlos Tavares Carvalho; Rosa H.V. Mourão; Raimundo N.P. Souto; Caio P. Fernandes. 2019. "A herbal oil in water nano-emulsion prepared through an ecofriendly approach affects two tropical disease vectors." Revista Brasileira de Farmacognosia 29, no. 6: 778-784.

Journal article
Published: 24 May 2019 in Toxins
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Variability in snake venom composition has been frequently reported and correlated to the adaptability of snakes to environmental conditions. Previous studies report plasticity for the venom phenotype. However, these observations are not conclusive, as the results were based on pooled venoms, which present high individual variability. Here we tested the hypothesis of plasticity by influence of confinement and single diet type in the venom composition of 13 adult specimens of Bothrops atrox snakes, maintained under captivity for more than three years. Individual variability in venom composition was observed in samples extracted just after the capture of the snakes. However, composition was conserved in venoms periodically extracted from nine specimens, which presented low variability restricted to the less abundant components. In a second group, composed of four snakes, drastic changes were observed in the venom samples extracted at different periods, mostly related to snake venom metalloproteinases (SVMPs), the core function toxins of B. atrox venom, which occurred approximately between 400 and 500 days in captivity. These data show plasticity in the venom phenotype during the lifetime of adult snakes maintained under captive conditions. Causes or functional consequences involved in the phenotype modification require further investigations.

ACS Style

Diana R. Amazonas; Luciana A. Freitas-De-Sousa; Daniele P. Orefice; Leijiane F. De Sousa; Melissa G. Martinez; Rosa H. V. Mourão; Hipócrates M. Chalkidis; Plínio B. Camargo; Ana M. Moura-Da-Silva. Evidence for Snake Venom Plasticity in a Long-Term Study with Individual Captive Bothrops atrox. Toxins 2019, 11, 294 .

AMA Style

Diana R. Amazonas, Luciana A. Freitas-De-Sousa, Daniele P. Orefice, Leijiane F. De Sousa, Melissa G. Martinez, Rosa H. V. Mourão, Hipócrates M. Chalkidis, Plínio B. Camargo, Ana M. Moura-Da-Silva. Evidence for Snake Venom Plasticity in a Long-Term Study with Individual Captive Bothrops atrox. Toxins. 2019; 11 (5):294.

Chicago/Turabian Style

Diana R. Amazonas; Luciana A. Freitas-De-Sousa; Daniele P. Orefice; Leijiane F. De Sousa; Melissa G. Martinez; Rosa H. V. Mourão; Hipócrates M. Chalkidis; Plínio B. Camargo; Ana M. Moura-Da-Silva. 2019. "Evidence for Snake Venom Plasticity in a Long-Term Study with Individual Captive Bothrops atrox." Toxins 11, no. 5: 294.

Journal article
Published: 10 December 2018 in Journal of Ethnopharmacology
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Oils and extracts of Eugenia uniflora have been reported as antimicrobial, antifungal, antinociceptive, antiprotozoal, antioxidant and cytotoxic. The oils of five specimens (E1 to E5) that occur in the Brazilian Amazon were extracted, analyzed for their chemical composition, and submitted to antioxidant and cytotoxic assays. Oils were hydrodistilled, analyzed by GC and GC-MS, and submitted to PCA and HCA analyses. The antioxidant activity of the oils was evaluated by the DPPH radical scavenging and the β-carotene/linoleic acid assays. Antiproliferative effects of the oils and curzerene were tested against colon (HCT-116), gastric (AGP-01), and melanoma (SKMEL-19) human cancer cell lines and a normal human fibroblast cell line (MRC-5), using MTT assay. Oxygenated sesquiterpenes and sesquiterpene hydrocarbons such as curzerene, selina-1,3,7(11)-trien-2-one, selina-1,3,7(11)-trien-2-one epoxide, germacrene B, caryophyllene oxide, and (E)-caryophyllene were predominant in the oils. PCA and HCA analyses classified the oils samples into four chemotypes. TEAC values of chemotype II (E3 oil, 228.3±19.2 mg TE/mL) and chemotype III (E4 oil, 217.0±23.3 mg TE/mL) displayed significant antioxidant activities. The oils E2 and E4 showed cytotoxic activity against all cell lines tested HCT-116 (IC50 E2: 16.26 μg/mL; IC50 E4: 9.28 μg/mL), AGP-01, (IC50 E2: 12.60 μg/mL; IC50 E4: 8.73 μg/mL), SKMEL-19 (IC50 E2: 12.20 μg/mL; IC50 E4: 15.42 μg/mL), and MRC-5 (IC50 E2: 10.27 μg/mL; IC50 E4: 14.95 μg/mL). Curzerene showed the more significant activity against melanoma cells (SKMEL-19, IC50: 5.17 μM), induced apoptosis at 5.0 μM and 10.0 μM compared to DMSO, exhibiting a decrease in the cell migration at 5.0 μM and 10.0 μM, after 30 h of treatment. The curzerene chemotype oil and E. uniflora oils can be indicated as drug candidates for anticancer activity of the lung, colon, stomach, and melanoma, with a real prospect to their subsequent phytotherapeutic development.

ACS Style

Pablo Luis B. Figueiredo; Laine C. Pinto; Jamile S. da Costa; Alberto Ray C. da Silva; Rosa Helena V. Mourão; Raquel C. Montenegro; Joyce Kelly R. da Silva; José Guilherme S. Maia. Composition, antioxidant capacity and cytotoxic activity of Eugenia uniflora L. chemotype-oils from the Amazon. Journal of Ethnopharmacology 2018, 232, 30 -38.

AMA Style

Pablo Luis B. Figueiredo, Laine C. Pinto, Jamile S. da Costa, Alberto Ray C. da Silva, Rosa Helena V. Mourão, Raquel C. Montenegro, Joyce Kelly R. da Silva, José Guilherme S. Maia. Composition, antioxidant capacity and cytotoxic activity of Eugenia uniflora L. chemotype-oils from the Amazon. Journal of Ethnopharmacology. 2018; 232 ():30-38.

Chicago/Turabian Style

Pablo Luis B. Figueiredo; Laine C. Pinto; Jamile S. da Costa; Alberto Ray C. da Silva; Rosa Helena V. Mourão; Raquel C. Montenegro; Joyce Kelly R. da Silva; José Guilherme S. Maia. 2018. "Composition, antioxidant capacity and cytotoxic activity of Eugenia uniflora L. chemotype-oils from the Amazon." Journal of Ethnopharmacology 232, no. : 30-38.

Aquaculture
Published: 22 October 2018 in Revista Brasileira de Zootecnia
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This study investigated the efficacy of essential oils of Lippia alba (EOLA) citral chemotype and Lippia origanoides (EOLO) and their effects on ventilatory frequency (VF) of silver catfish, Rhamdia quelen. Fish were exposed to 50-300 μL L−1 of EOLA and 25-300 μL L−1 of EOLO to determine induction times to sedation, anesthesia, and recovery. Moreover, VF was determined in fish exposed to 5 or 10 μL L−1 of EOLA and of EOLO for 8 h. The increasing concentration of essential oils proportionally decreased sedation and anesthesia induction times. The highest EOLA concentration increased VF of fish from the control group at 1 h of exposure, but VF decreased at both EOLO concentrations after 2 h. The EOLA citral chemotype and EOLO were effective sedatives and anesthetics for silver catfish. However, EOLO was the most suitable sedative for additional studies regarding fish transport as it reduced VF and did not induce VF increase in silver catfish. The EOLA citral chemotype and EOLO are effective sedatives and anesthetics for silver catfish. Moreover, the EOLO is recommended for transport of silver catfish, because it maintains the ventilatory frequency constant, avoiding a possible metabolic stress.

ACS Style

Alessandra Janaína Becker; Carine De Oliveira Fogliarini; Carine De Freitas Souza; Alexssandro Geferson Becker; Rosa Helena Veras Mourão; Lenise Vargas Flôres Da Silva; Bernardo Baldisserotto. Ventilatory frequency and anesthetic efficacy in silver catfish, Rhamdia quelen: a comparative approach between different essential oils. Revista Brasileira de Zootecnia 2018, 47, 1 .

AMA Style

Alessandra Janaína Becker, Carine De Oliveira Fogliarini, Carine De Freitas Souza, Alexssandro Geferson Becker, Rosa Helena Veras Mourão, Lenise Vargas Flôres Da Silva, Bernardo Baldisserotto. Ventilatory frequency and anesthetic efficacy in silver catfish, Rhamdia quelen: a comparative approach between different essential oils. Revista Brasileira de Zootecnia. 2018; 47 ():1.

Chicago/Turabian Style

Alessandra Janaína Becker; Carine De Oliveira Fogliarini; Carine De Freitas Souza; Alexssandro Geferson Becker; Rosa Helena Veras Mourão; Lenise Vargas Flôres Da Silva; Bernardo Baldisserotto. 2018. "Ventilatory frequency and anesthetic efficacy in silver catfish, Rhamdia quelen: a comparative approach between different essential oils." Revista Brasileira de Zootecnia 47, no. : 1.

Journal article
Published: 01 March 2018 in Journal of Ethnopharmacology
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Extracts of leaves and bark of Aniba fragrans are used as tea (decoction) to treat snakebites in communities in the Brazilian Amazon. The aqueous extract of the leaves of A. fragrans has been proven to be effective against Bothrops venom, but only when pre-incubated with the venom. This study sought to assess the potential of different types of extract of this species to inhibit the biological activities of Bothrops atrox venom (BaV) when used the same way as in folk medicine. The main classes of secondary metabolites and the concentrations of phenolics in the extracts were also determined.Four types of extract of A. fragrans were prepared: aqueous extract of the leaf (AEL), aqueous extract of the bark (AEB), hydroalcoholic leaf extract (HLE) and extract of the residue from hydrodistillation of the leaf (ERHL). The phytochemical profiles of the aqueous extracts were determined using thin layer chromatography (TLC), and the concentrations of phenolics were measured by colorimetric assays. To investigate the potential of the extracts to inhibit the biological activities of BaV, in vitro tests for antiphospholipase and antifibrinolytic activities were performed. In vivo tests for antihemorrhagic and antidefibrinating activities were also carried out, as well as antimicrobial tests for activity against the main bacteria found in the oral cavity of snakes. Interaction between the extracts and the proteins in BaV was assessed by electrophoresis (SDS-PAGE) and Western blot (WB). The cytotoxicity of the extracts was assessed in a strain of MRC-5 human fibroblasts.Terpenoids, flavonoids and condensed and hydrolysable tannins were detected in all the extracts. Metabolites such as coumarins, fatty acids and alkaloids were present in some extracts but not in others, indicating different phytochemical profiles. Phenolics content varied between extracts, and there were more tannins in AEB and HLE. In the in vitro tests, the extracts inhibited the phospholipase and fibrinolytic activities of BaV in the two ratios of venom to extract used. HLE exhibited effective antimicrobial action as it inhibited growth of 11 of the 15 bacteria investigated, including Morganella morganii, the main bacteria described in the oral cavity of snakes. The extracts failed to inhibit the defibrinating activity of BaV, and only the Bothrops antivenom had a significant effect (96.1%) on this activity. BaV-induced hemorrhage was completely inhibited by AEL and AEB when the pre-incubation (venom:extract) protocol was used. When administered orally, as in folk medicine, both AEB and AEL produced significant inhibition of hemorrhagic activity (maximum inhibition 46.5% and 39.2%, respectively). SDS-PAGE and WB of the extracts pre-incubated with BaV showed that the main proteins in the venom had been precipitated by the extracts. None of the four extracts showed cytotoxic effects in the tests carried out with a human fibroblast cell line.In addition to being effective in reducing hemorrhage when administered orally, the extracts displayed a high antimicrobial potential against microorganisms involved in secondary infections at the site of the snakebite. Once the extracts have been tested in accordance with the appropriate regulations, this species could potentially be used to produce a phytomedicine for complementary treatment of the secondary infections due to bacteria that aggravate the local signs and symptoms after snakebite envenomation.

ACS Style

Valéria Mourão de Moura; Noranathan Da Costa Guimarães; Luana Travassos Batista; Luciana A. Freitas-De-Sousa; Joanderson De Sousa Martins; Maria Carolina Scheffer de Souza; Patrícia D. Oliveira de Almeida; Wuelton Marcelo Monteiro; Ricardo Bezerra de Oliveira; Maria Cristina Dos-Santos; Rosa Helena Veras Mourão. Assessment of the anti-snakebite properties of extracts of Aniba fragrans Ducke (Lauraceae) used in folk medicine as complementary treatment in cases of envenomation by Bothrops atrox. Journal of Ethnopharmacology 2018, 213, 350 -358.

AMA Style

Valéria Mourão de Moura, Noranathan Da Costa Guimarães, Luana Travassos Batista, Luciana A. Freitas-De-Sousa, Joanderson De Sousa Martins, Maria Carolina Scheffer de Souza, Patrícia D. Oliveira de Almeida, Wuelton Marcelo Monteiro, Ricardo Bezerra de Oliveira, Maria Cristina Dos-Santos, Rosa Helena Veras Mourão. Assessment of the anti-snakebite properties of extracts of Aniba fragrans Ducke (Lauraceae) used in folk medicine as complementary treatment in cases of envenomation by Bothrops atrox. Journal of Ethnopharmacology. 2018; 213 ():350-358.

Chicago/Turabian Style

Valéria Mourão de Moura; Noranathan Da Costa Guimarães; Luana Travassos Batista; Luciana A. Freitas-De-Sousa; Joanderson De Sousa Martins; Maria Carolina Scheffer de Souza; Patrícia D. Oliveira de Almeida; Wuelton Marcelo Monteiro; Ricardo Bezerra de Oliveira; Maria Cristina Dos-Santos; Rosa Helena Veras Mourão. 2018. "Assessment of the anti-snakebite properties of extracts of Aniba fragrans Ducke (Lauraceae) used in folk medicine as complementary treatment in cases of envenomation by Bothrops atrox." Journal of Ethnopharmacology 213, no. : 350-358.

Journal article
Published: 01 January 2018 in Journal of Experimental Biology
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Lippia alba is a flowering shrub in the verbena family and its essential oil (EO) is known for its sedative, antidepressant and analgesic properties. In the Amazon region of Brazil, it is used in aquaculture to anesthetize fish during transport. Many of the specialized metabolites found in EOs presumably evolved to protect plants from herbivores, especially insects. We used Drosophila to test the behavioral and physiological actions of this EO and its components. We found that 150 min exposure to the EO vapors resulted in immobilization of adult flies. Gas chromatography-mass spectrometry identified the major components of the EO as the monoterpenes: citral (59%), carvone (7%) and limonene (7%). Fly immobilization by the EO was due to citral and carvone with citral producing more rapid effects than carvone. We tested whether the EO affected synaptic physiology by applying it to the larval neuromuscular junction. The EO delivered at 0.012% (v/v) produced over a 50% reduction in EPSP amplitude within 3-4 min. When the EO components were applied at 0.4 mM, citral and carvone produced a significant reduction in EPSP amplitude with citral producing the largest effect. Measurement of miniature EPSP amplitudes demonstrated that citral produced over a 50% reduction in transmitter release. Ca-imaging experiments showed that citral produced about 30% reduction in presynaptic Ca influx, which likely resulted in the decrease in transmitter release. Thus, the EO blocks synaptic transmission, largely due to citral, and this likely contributes to its behavioral effects.

ACS Style

Lenise Vargas Flores da Silva; Rosa Helena Veras Mourão; Jibin Manimala; Gregory A. Lnenicka. The essential oil of Lippia alba and its components affect Drosophila behavior and synaptic physiology. Journal of Experimental Biology 2018, 221, jeb.176909 .

AMA Style

Lenise Vargas Flores da Silva, Rosa Helena Veras Mourão, Jibin Manimala, Gregory A. Lnenicka. The essential oil of Lippia alba and its components affect Drosophila behavior and synaptic physiology. Journal of Experimental Biology. 2018; 221 ():jeb.176909.

Chicago/Turabian Style

Lenise Vargas Flores da Silva; Rosa Helena Veras Mourão; Jibin Manimala; Gregory A. Lnenicka. 2018. "The essential oil of Lippia alba and its components affect Drosophila behavior and synaptic physiology." Journal of Experimental Biology 221, no. : jeb.176909.

Journal article
Published: 01 January 2018 in Integrative Food, Nutrition and Metabolism
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OA Text is an independent open-access scientific publisher showcases innovative research and ideas aimed at improving health by linking research and practice to the benefit of society.

ACS Style

Lais Tatiele Massing; Rosa Helena V. Mourão; Leoneide Erica M. Bouillet; Regiane Sablina A. Bernardes; Eloísa Helena A. Andrade; Agnaldo Tremea; Jesus Nazareno S. Souza; José Guilherme S. Maia. Nutritional composition of the pulp of Pajura (Couepia bracteosa Benth.), an underutilized fruit from the Amazon. Integrative Food, Nutrition and Metabolism 2018, 5, 1 .

AMA Style

Lais Tatiele Massing, Rosa Helena V. Mourão, Leoneide Erica M. Bouillet, Regiane Sablina A. Bernardes, Eloísa Helena A. Andrade, Agnaldo Tremea, Jesus Nazareno S. Souza, José Guilherme S. Maia. Nutritional composition of the pulp of Pajura (Couepia bracteosa Benth.), an underutilized fruit from the Amazon. Integrative Food, Nutrition and Metabolism. 2018; 5 (2):1.

Chicago/Turabian Style

Lais Tatiele Massing; Rosa Helena V. Mourão; Leoneide Erica M. Bouillet; Regiane Sablina A. Bernardes; Eloísa Helena A. Andrade; Agnaldo Tremea; Jesus Nazareno S. Souza; José Guilherme S. Maia. 2018. "Nutritional composition of the pulp of Pajura (Couepia bracteosa Benth.), an underutilized fruit from the Amazon." Integrative Food, Nutrition and Metabolism 5, no. 2: 1.

Biology
Published: 27 November 2017 in Ciência Rural
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The goal of this study was to investigate the sedative and anesthetic properties of essential oils (EOs) in map treefrog tadpoles (Hypsiboas geographicus) and to determine the sedation and deep anesthesia induction times as well as the recovery time. The tadpoles were exposed to one of the EOs from three plant species: Aniba rosaeodora (EOAR - 25, 50, 100 or 200µL L-1), Lippia origanoides (EOLO - 13, 25, 50, 100 or 200µL L-1), and Lippia alba (either chemotype citral [EOL-C - 25, 50, 100 or 200µL L-1] or linalool [EOL-L - 50, 75, 100 or 200µL L-1]) (n = 8 per replicate). The tadpoles exposed to 25 and 50µL L-1 EOL-C and EOL-L, respectively, were not anesthetized within 30min (the maximum time of observation), and those exposed to 200µL L-1 EOLO did not recover within 30min. Sedation, deep anesthesia and recovery times showed a concentration-dependent relationship for all EOs tested, with the exception of the recovery with EOLO. The results allowed concluding that all investigated EOs can be used to anesthetize tadpoles of H. geographicus, but the use of EOLO must not exceed 100µL L-1. RESUMO: O objetivo deste estudo foi investigar as propriedades sedativas e anestésicas de óleos essenciais (OEs) em girinos da perereca Hypsiboas geographicus e determinar os tempos de indução à sedação e anestesia profunda, bem como o de recuperação. Os girinos foram expostos a um dos OEs de três espécies de plantas: Aniba rosaeodora (OEAR - 25, 50, 100 ou 200µL L-1), Lippia origanoides (OELO - 13, 25, 50, 100 ou 200µL L-1 ) ou Lippia alba quimiotipos citral (OEL-C - 25, 50, 100 ou 200µL L-1) ou linalol (OEL-L - 50, 75, 100 ou 200µL L-1) (n = 8 cada repetição). Girinos expostos a 25 e 50µL L-1 OEL-C e OEL-L, respectivamente, não foram anestesiados dentro de 30min (tempo máximo de observação) e aqueles expostos a 200µL L-1 OELO não recuperaram dentro de 30min. Os tempos de sedação, anestesia profunda e recuperação apesentaram uma relação concentração-resposta para todos os OEs testadas, exceto a recuperação com OELO. Os resultados permitem concluir que todos os OEs investigados podem ser usados para anestesiar os girinos de H. geographicus, mas o uso de OELO não deve ser superior a 100µL L-1.

ACS Style

Joseânia Salbego; Janna Laely Dos Santos Maia; Cândida Toni; Amanda Sousa Silva Rodrigues; Elen Monique Oliveira Sousa; Lenise Vargas Flores Da Silva; Rosa Helena Veras Mourão; Lauro Euclides Soares Barata; Berta Maria Heinzmann; Bernardo Baldisserotto. Anesthesia and sedation of map treefrog (Hypsiboas geographicus) tadpoles with essential oils. Ciência Rural 2017, 47, 1 .

AMA Style

Joseânia Salbego, Janna Laely Dos Santos Maia, Cândida Toni, Amanda Sousa Silva Rodrigues, Elen Monique Oliveira Sousa, Lenise Vargas Flores Da Silva, Rosa Helena Veras Mourão, Lauro Euclides Soares Barata, Berta Maria Heinzmann, Bernardo Baldisserotto. Anesthesia and sedation of map treefrog (Hypsiboas geographicus) tadpoles with essential oils. Ciência Rural. 2017; 47 (11):1.

Chicago/Turabian Style

Joseânia Salbego; Janna Laely Dos Santos Maia; Cândida Toni; Amanda Sousa Silva Rodrigues; Elen Monique Oliveira Sousa; Lenise Vargas Flores Da Silva; Rosa Helena Veras Mourão; Lauro Euclides Soares Barata; Berta Maria Heinzmann; Bernardo Baldisserotto. 2017. "Anesthesia and sedation of map treefrog (Hypsiboas geographicus) tadpoles with essential oils." Ciência Rural 47, no. 11: 1.

Journal article
Published: 25 July 2017 in Aquaculture Research
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Lippia alba hydrolate (LAH) is produced as a secondary product in essential oil extraction. The objective of this study was to evaluate the use of LAH in a simulation of the transport of tambaqui (Colossoma macropomum) juveniles. A series of 17 hr simulations were performed using load densities of 30, 60 and 90 juveniles per L and four LAH concentrations of 0.4%, 1%, 2% and 5%, with a control of just water. Fish survival, temperature, dissolved oxygen, conductivity, pH, ammonia excretion, alkalinity water hardness and net Na+, K+, Cl- fluxes, were all evaluated before and after the transport simulations. Mucous cell densities in the gills were also determined microscopically, post simulation. The results showed that addition of 5% LAH achieved better survival during the simulation than the water control. Dissolved oxygen was significantly lower with an increased load density. Also, conductivity, NH4 excretion and net ionic fluxes (K+ and Cl−) were lower with 5% LAH, although mainly where higher load densities were tested. Mucous cell density was lower in LAH concentrations of 2% and 5%, again with higher load densities. In view of these findings, it is suggested that 5% LAH is an effective sedative for use in the transport of tambaqui, mainly with high load densities. This work is the first study of LAH and highlights its potential applications in aquaculture management.

ACS Style

Hugo Napoleão Pereira Da Silva; Elen Monique De Oliveira Sousa; Janna Laely Dos Santos Maia; Mônica Tatiane Lima Pinheiro; Soraia Valeria De Oliveira Coelho Lameirão; Rosa Helena Veras Mourão; José Guilherme Soares Maia; Bernardo Baldisserotto; Lenise Vargas Flores Da Silva. Lippia alba(Verbenaceae) hydrolate as sedative of tambaqui (Colossoma macropomum) juveniles in simulated transport conditions. Aquaculture Research 2017, 49, 128 -134.

AMA Style

Hugo Napoleão Pereira Da Silva, Elen Monique De Oliveira Sousa, Janna Laely Dos Santos Maia, Mônica Tatiane Lima Pinheiro, Soraia Valeria De Oliveira Coelho Lameirão, Rosa Helena Veras Mourão, José Guilherme Soares Maia, Bernardo Baldisserotto, Lenise Vargas Flores Da Silva. Lippia alba(Verbenaceae) hydrolate as sedative of tambaqui (Colossoma macropomum) juveniles in simulated transport conditions. Aquaculture Research. 2017; 49 (1):128-134.

Chicago/Turabian Style

Hugo Napoleão Pereira Da Silva; Elen Monique De Oliveira Sousa; Janna Laely Dos Santos Maia; Mônica Tatiane Lima Pinheiro; Soraia Valeria De Oliveira Coelho Lameirão; Rosa Helena Veras Mourão; José Guilherme Soares Maia; Bernardo Baldisserotto; Lenise Vargas Flores Da Silva. 2017. "Lippia alba(Verbenaceae) hydrolate as sedative of tambaqui (Colossoma macropomum) juveniles in simulated transport conditions." Aquaculture Research 49, no. 1: 128-134.

Articles
Published: 01 January 2017 in Neotropical Ichthyology
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The aim of this study was to evaluate if Lippia alba has different chemotypes according to the chemical composition of the essential oil (EO) considering collection site, and if the EO may have different effects on blood and plasma parameters in silver catfish, Rhamdia quelen, during and immediately after anesthesia. The citral (EO-C) and linalool (EO-L) chemotypes were identified, and both presented similar anesthetic effects for silver catfish. Fish were exposed to two concentrations of each EO, which induced slow and fast anesthesia (100 and 300 µL L-1, respectively). Blood ions did not change at any time of anesthesia induction and recovery and, therefore, the electrolyte balance was not altered. Blood gases oscillated through all exposure and recovery times, but there was an increase in pO2 after 10 min recovery in fish anesthetized with EO-C. Glucose increased in fish exposed to both EOs when compared with the control group. Overall, exposure to both EOs (except 100 µL L-1 EO-L at most times) reduced plasma cortisol levels compared to the control and/or ethanol groups. However, as plasma creatinine levels in fish anesthetized with EO-C were higher than control fish, the use of EO-L is preferable.

ACS Style

Carine De Freitas Souza; Matheus Dellaméa Baldissera; Joseânia Salbego; Jane Mello Lopes; Rodrigo De Almeida Vaucher; Rosa Helena Veras Mourão; Braulio O. Caron; Berta Maria Heinzmann; Lenise Vargas Flôres Da Silva; Bernardo Baldisserotto. Physiological responses of Rhamdia quelen (Siluriformes: Heptapteridae) to anesthesia with essential oils from two different chemotypes of Lippia alba. Neotropical Ichthyology 2017, 15, 1 .

AMA Style

Carine De Freitas Souza, Matheus Dellaméa Baldissera, Joseânia Salbego, Jane Mello Lopes, Rodrigo De Almeida Vaucher, Rosa Helena Veras Mourão, Braulio O. Caron, Berta Maria Heinzmann, Lenise Vargas Flôres Da Silva, Bernardo Baldisserotto. Physiological responses of Rhamdia quelen (Siluriformes: Heptapteridae) to anesthesia with essential oils from two different chemotypes of Lippia alba. Neotropical Ichthyology. 2017; 15 (1):1.

Chicago/Turabian Style

Carine De Freitas Souza; Matheus Dellaméa Baldissera; Joseânia Salbego; Jane Mello Lopes; Rodrigo De Almeida Vaucher; Rosa Helena Veras Mourão; Braulio O. Caron; Berta Maria Heinzmann; Lenise Vargas Flôres Da Silva; Bernardo Baldisserotto. 2017. "Physiological responses of Rhamdia quelen (Siluriformes: Heptapteridae) to anesthesia with essential oils from two different chemotypes of Lippia alba." Neotropical Ichthyology 15, no. 1: 1.

Journal article
Published: 10 January 2016 in European Journal of Medicinal Plants
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Leomara A. Da Silva; Sandra Layse F. Sarrazin; Ricardo B. Oliveira; Chieno Suemitsu; José Guilherme S. Maia; Rosa Helena V. Mourão. Composition and Antimicrobial Activity of Leaf Essential Oils of Myrcia sylvatica (G. Mey.) DC. European Journal of Medicinal Plants 2016, 13, 1 -9.

AMA Style

Leomara A. Da Silva, Sandra Layse F. Sarrazin, Ricardo B. Oliveira, Chieno Suemitsu, José Guilherme S. Maia, Rosa Helena V. Mourão. Composition and Antimicrobial Activity of Leaf Essential Oils of Myrcia sylvatica (G. Mey.) DC. European Journal of Medicinal Plants. 2016; 13 (3):1-9.

Chicago/Turabian Style

Leomara A. Da Silva; Sandra Layse F. Sarrazin; Ricardo B. Oliveira; Chieno Suemitsu; José Guilherme S. Maia; Rosa Helena V. Mourão. 2016. "Composition and Antimicrobial Activity of Leaf Essential Oils of Myrcia sylvatica (G. Mey.) DC." European Journal of Medicinal Plants 13, no. 3: 1-9.

Journal article
Published: 01 January 2016 in Revista Fitos
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Adrielle Nara Bezerra; Ricardo B. De Oliveira; Rodrigo Da Silva; Jose Guilherme Soares Maia; Rosa Helena V. Mourão. Light impact assessment in planting and production of Curcuma longa in the Amazon, based on the analysis of its essential oils from leaves and rhizomes. Revista Fitos 2016, 10, 1 .

AMA Style

Adrielle Nara Bezerra, Ricardo B. De Oliveira, Rodrigo Da Silva, Jose Guilherme Soares Maia, Rosa Helena V. Mourão. Light impact assessment in planting and production of Curcuma longa in the Amazon, based on the analysis of its essential oils from leaves and rhizomes. Revista Fitos. 2016; 10 (3):1.

Chicago/Turabian Style

Adrielle Nara Bezerra; Ricardo B. De Oliveira; Rodrigo Da Silva; Jose Guilherme Soares Maia; Rosa Helena V. Mourão. 2016. "Light impact assessment in planting and production of Curcuma longa in the Amazon, based on the analysis of its essential oils from leaves and rhizomes." Revista Fitos 10, no. 3: 1.

Journal article
Published: 09 November 2015 in Lipids in Health and Disease
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Lippia origanoides Kunth from Northeast Brazil is a plant of pleasant odor used by local people as a food seasoning in substitution the oregano where its carvacrol-rich oil has showed significant antimicrobial activity against human pathogens. GC and GC-MS analyzed the plant oil composition and its antibacterial activity was evaluated by disk diffusion and microdilution broth methods. The determination of oil antioxidant activity was made by DPPH radical scavenging assay. Oil toxicity was performed on mice. The main constituents of the oil were carvacrol (47.2 %), thymol (12.8 %), p-cymene (9.7 %), and p-methoxythymol (7.4 %). The oil was active against the bacteria of Bacillus cereus, B. subtilis, and Salmonella typhimurium, except for Pseudomonas aeruginosa. The antioxidant activity has displayed a high dose–response (r2 = 0.92), with the inhibition of DPPH radical from 15 to 82 %, at concentrations from 5 to 50 μg/mL, and also by the β-carotene bleaching assay, which showed a high inhibition of 85.2 ± 6.8 %, corresponding to about 80 % of the inhibition of Trolox (93.4 ± 0.7 %), used as a standard. The lethal dose (LD50) of the oil was determined in 1673.84 mg mL−1. The results confirmed that the oil of L. origanoides could be utilized for the prevention of food bacterial growth, and as an antioxidative agent for retardation of food oxidation process. The oil has low toxicity, allowing its application in the food industry. Graphical Abstract Aerial parts of Lippia origanoides Kunth

ACS Style

Sandra Layse F. Sarrazin; Leomara A. Da Silva; Ricardo B. Oliveira; Juliana Divina A. Raposo; Joyce Kelly R. Da Silva; Fátima Regina G. Salimena; José Guilherme S. Maia; Rosa Helena V. Mourão. Antibacterial action against food-borne microorganisms and antioxidant activity of carvacrol-rich oil from Lippia origanoides Kunth. Lipids in Health and Disease 2015, 14, 145 .

AMA Style

Sandra Layse F. Sarrazin, Leomara A. Da Silva, Ricardo B. Oliveira, Juliana Divina A. Raposo, Joyce Kelly R. Da Silva, Fátima Regina G. Salimena, José Guilherme S. Maia, Rosa Helena V. Mourão. Antibacterial action against food-borne microorganisms and antioxidant activity of carvacrol-rich oil from Lippia origanoides Kunth. Lipids in Health and Disease. 2015; 14 (1):145.

Chicago/Turabian Style

Sandra Layse F. Sarrazin; Leomara A. Da Silva; Ricardo B. Oliveira; Juliana Divina A. Raposo; Joyce Kelly R. Da Silva; Fátima Regina G. Salimena; José Guilherme S. Maia; Rosa Helena V. Mourão. 2015. "Antibacterial action against food-borne microorganisms and antioxidant activity of carvacrol-rich oil from Lippia origanoides Kunth." Lipids in Health and Disease 14, no. 1: 145.

Journal article
Published: 23 January 2015 in Molecules
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This study evaluated the influence of seasonal variation on the yield and composition of essential oil of Lippia origanoides occurring in the Middle Rio Amazonas, Brazil, and the impact on its antimicrobial potential. The average oil yield was 1.7% ± 0.2% in the rainy season and 1.6% ± 0.3% in the dry season. Some correlations with climatic parameters were observed. The major components were carvacrol (rainy, 43.5% ± 1.9%; dry, 41.4% ± 2.04%), thymol (rainy, 10.7% ± 1.1%; dry, 10.6% ± 0.9%), p-cymene (rainy, 9.8% ± 0.7%; dry, 10.0% ± 1.4%) and p-methoxythymol (rainy, 9.6% ± 0.8%; dry, 10.4% ± 1.4%). It was found that the antibacterial activity of L. origanoides against Staphylococcus aureus and Escherichia coli was little influenced by the changes in oil composition due to seasonal variation. Against S. aureus, the oil Minimum Inhibitory Concentration (MIC) value was 1.25 μL/mL over ten months. Against E. coli, the oil MIC values ranged from 0.15 μL/mL to 0.31 μL/mL in different months of the year. The Minimum Bactericidal Concentration (MBC) value was 2.5 μL/mL against S. aureus and 1.25 μL/mL against E. coli. The results suggest that the antimicrobial activity identified in the oil remain unchanged for the full year, allowing its medicinal use without any risk of loss or absence of the active principles of the plant.

ACS Style

Sandra Layse F. Sarrazin; Leomara Andrade Da Silva; Ana Paula F. De Assunção; Ricardo B. Oliveira; Victor Y. P. Calao; Rodrigo Da Silva; Elena E. Stashenko; José Guilherme S. Maia; Rosa Helena V. Mourão. Antimicrobial and Seasonal Evaluation of the Carvacrol-Chemotype Oil from Lippia origanoides Kunth. Molecules 2015, 20, 1860 -1871.

AMA Style

Sandra Layse F. Sarrazin, Leomara Andrade Da Silva, Ana Paula F. De Assunção, Ricardo B. Oliveira, Victor Y. P. Calao, Rodrigo Da Silva, Elena E. Stashenko, José Guilherme S. Maia, Rosa Helena V. Mourão. Antimicrobial and Seasonal Evaluation of the Carvacrol-Chemotype Oil from Lippia origanoides Kunth. Molecules. 2015; 20 (2):1860-1871.

Chicago/Turabian Style

Sandra Layse F. Sarrazin; Leomara Andrade Da Silva; Ana Paula F. De Assunção; Ricardo B. Oliveira; Victor Y. P. Calao; Rodrigo Da Silva; Elena E. Stashenko; José Guilherme S. Maia; Rosa Helena V. Mourão. 2015. "Antimicrobial and Seasonal Evaluation of the Carvacrol-Chemotype Oil from Lippia origanoides Kunth." Molecules 20, no. 2: 1860-1871.