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SARS-CoV-2 is highly pathogenic in humans and poses a great threat to public health worldwide. Clinical data shows a disturbed type I interferon (IFN) response during the virus infection. In this study, we discovered that the nucleocapsid (N) protein of SARS-CoV-2 plays an important role in the inhibition of interferon beta (IFN-β) production. N protein repressed IFN-β production induced by poly(I:C) or upon Sendai virus (SeV) infection. We noted that N protein also suppressed IFN-β production, induced by several signaling molecules downstream of the retinoic acid-inducible gene I (RIG-I) pathway, which is the crucial pattern recognition receptor (PRR) responsible for identifying RNA viruses. Moreover, our data demonstrated that N protein interacted with the RIG-I protein through the DExD/H domain, which has ATPase activity and plays an important role in the binding of immunostimulatory RNAs. These results suggested that SARS-CoV-2 N protein suppresses the IFN-β response through targeting the initial step, potentially the cellular PRR–RNA-recognition step in the innate immune pathway. Therefore, we propose that the SARS-CoV-2 N protein represses IFN-β production by interfering with RIG-I.
Keli Chen; Feng Xiao; Dingwen Hu; Weiwei Ge; Mingfu Tian; Wenbiao Wang; Pan Pan; Kailang Wu; Jianguo Wu. SARS-CoV-2 Nucleocapsid Protein Interacts with RIG-I and Represses RIG-Mediated IFN-β Production. Viruses 2020, 13, 47 .
AMA StyleKeli Chen, Feng Xiao, Dingwen Hu, Weiwei Ge, Mingfu Tian, Wenbiao Wang, Pan Pan, Kailang Wu, Jianguo Wu. SARS-CoV-2 Nucleocapsid Protein Interacts with RIG-I and Represses RIG-Mediated IFN-β Production. Viruses. 2020; 13 (1):47.
Chicago/Turabian StyleKeli Chen; Feng Xiao; Dingwen Hu; Weiwei Ge; Mingfu Tian; Wenbiao Wang; Pan Pan; Kailang Wu; Jianguo Wu. 2020. "SARS-CoV-2 Nucleocapsid Protein Interacts with RIG-I and Represses RIG-Mediated IFN-β Production." Viruses 13, no. 1: 47.