A misalignment fault is a kind of potential fault in double-fed wind turbines. The reasonable and effective fault prediction models are used to predict its development trend before serious faults occur, which can take measures to repair in advance and reduce human and material losses. In this paper, the Least Squares Support Vector Machine optimized by the Improved Artificial Fish Swarm Algorithm is used to predict the misalignment index of the experiment platform. The mixed features of time domain, frequency domain, and time-frequency domain indexes of vibration or stator current signals are the inputs of the Least Squares Support Vector Machine. The kurtosis of the same signals is the output of the model, and the $3\sigma$ principle of the normal distribution is adopted to set the warning line of misalignment fault. Compared with other optimization algorithms, the experimental results show that the proposed prediction model can predict the development trend of the misalignment index with the least prediction error.