Mitochondrial fission and fusion activities are important for cell survival and function. Drp1 is a GTPase protein responsible for mitochondrial division, and SAMM50 is responsible for protein sorting and assembly. We demonstrated that SAMM50 overexpression results in Drp1‐dependent mitochondrial fragmentation in HeLa cells. However, the mitochondrial fragmentation induced by SAMM50 overexpression could be reversed through co‐expression with MFN2. Furthermore, SAMM50 interacts with Drp1 both in vivo and in vitro. The mitochondria in SAMM50 knockdown HeLa cells displayed a swollen phenotype, and the levels of the SAM complex and OPA1, along with the mitochondrial Drp1 levels, significantly decreased. In addition, mitochondrial inheritance was impaired in SAMM50 silenced cells. These results suggest that SAMM50 affects the Drp1‐dependent mitochondrial morphology.

In order to investigate the effect of shot distance and impact sequence on the residual stress distribution of 42CrMo steel in shot peening (SP) finite element (FE) simulation, 3D dynamic models with order dimple pattern and stochastic dimple pattern were established via ABAQUS/Explicit 6.14, and the simulation results were compared with experiments. The results show that shot overlap has a significant effect on the residual stress distribution of peened parts. Meanwhile, there is a threshold (related to SP parameter) for shot distance in the vertical and horizontal directions. When the shot distance is greater than the threshold in this direction, the residual stress distribution after SP tends to be stable. The impact sequence has almost no effect on the impact of a small number of shots, but this effect will appear when the number of shots increases. It is necessary to avoid shot overlap and continuous impact of adjacent dimples when the FE model is established; on this basis, the distance between shots and the number of layers of the shots can be reduced as much as possible without affecting the residual stress distribution. In addition, the comparison of simulation and experimental results shows that the residual stress evaluation area consistent with the experimental measurement is essential to obtain accurate residual stress distribution in the FE simulation process.