Dr. Andrea Doni is the Head of the Imaging Unit at IRCCS Humanitas Research Hospital. Doni obtained a PhD in Immunology and Immunopathology from the Open University of London. From 1995 to 2005, he worked in the Department of Immunology directed by Professor Alberto Mantovani at the Pharmacological Institute Mario Negri of Milan, before moving in 2005 to IRCCS Humanitas Research Hospital. He specializes in studies of preclinical research with specific expertise in experimental in vivo models of humoral resistance against pathogens, in prognostic and predictive biomarker discovery of inflammatory diseases, and involvement of components of the innate immune system in tissue repair. Since 2002, he has dealt with advanced optical microscopy. He maintains and develops optical technologies for basic and advanced research, in linear (wide field fluorescence; Live Cell Imaging; confocal microscopy; FRAP, FLIP, TIRF, Photoactivation; Photoconversion; Uncaging) and non-linear (super-resolution STED; FLIM-based FRET; 2P excitation) microscopy. He developed different protocols and in vitro and in vivo experimental models for confocal and intravital optical microscopy analysis. His further current interest is in multiparametric spatial analysis of tissue using Imaging Mass Cytometry systems.