Methane (CH₄) production from ruminants accounts for 16% of the global greenhouse gas emissions and represents 2% to 12% of feed energy. Mitigating CH₄ production from ruminants is of great importance for sustainable development of the ruminant industry. H₂ is the primary substrate for CH₄ production in the processes of ruminal methanogenesis. Sulfate reducing bacteria are able to compete with methanogens for H₂ in the rumen, and consequently inhibit the methanogenesis. Enhancing the ruminal sulfate reducing pathway is an important approach to mitigate CH₄ emissions in ruminants. The review summarized the effects of sulfate and elemental S on ruminal methanogenesis, and clarified the related mechanisms through the impacts of sulfate and elemental S on major ruminal sulfate reducing bacteria. Enhancing the activities of the major ruminal sulfate reducing bacteria including Desulfovibrio, Desulfohalobium and Sulfolobus through dietary sulfate addition, elemental S and dried distillers grains with solubles can effectively decrease the ruminal CH₄ emissions. Suitable levels of dietary addition with different S sources for reducing the ruminal CH₄ production, as well as maintaining the performance and health of ruminants, need to be investigated in the future.

Six steers were used to study the effects of dietary supplementation with sodium sulfate (Na₂SO₄) on rumen fermentation, nutrient digestion, rumen microbiota and plasma metabolites. The animals were fed a basal ration added with Na₂SO₄ at 0 [sulfur (S) content 0.115% DM], 20 (S 0.185% DM) and 40 g/d (S 0.255%DM), respectively, in a replicate 3 × 3 Latin square design. Results indicated that supplementing Na₂SO₄ increased the ruminal concentration of total volatile fatty acids, the molar proportions of acetate and butyrate, the ruminal concentrations of microbial protein, SO₄^2--S and S^2--S and the digestibility of fiber while it decreased the molar proportion of propionate and the ruminal concentration of ammonia nitrogen. Supplementing Na₂SO₄ increased the diversity and the richness of rumen microbiota and the relative abundances of phylum Firmicutes, and genera Ruminococcus 2, Rikenellaceae RC9 gut group and Desulfovibrio whereas it decreased that of phylum Bacteroidetes and genera Prevotella 1, Prevotellaceae UCG-001 and Treponema 2. Supplementing Na₂SO₄ also increased the plasma concentrations of amino acids (L-arginine, L-methionine, L-cysteine, L-lysine), purine derivatives (xanthine, hypoxanthine), vitamins (thiamin, biotin) and lipids (acetylcarnitine and L-carnitine). It was concluded supplementation with Na₂SO₄ to steer ration was beneficial to improve the rumen fermentation, fiber digestibility and nutrient metabolism through modulating rumen microbial community. IMPORTANCE Essential elements such as nitrogen and sulfur greatly affect rumen fermentation and metabolism in ruminants. However, little knowledge is available on the effects of sulfur on rumen microbiota and plasma metabolome. Results of the present trial demonstrated that supplementation with sodium sulfate to steer ration markedly improved rumen fermentation, fiber digestibility and metabolism of amino acids, purine derivatives and vitamins through the impacts on the ruminal microbiome. The facts obtained from the present trial clarified the possible mechanisms of the positive effects of sulfur on rumen fermentation and nutrient utilization.

The objectives of the trial were to study the effects of dietary crude protein (CP) and tannic acid (TA) on rumen fermentation, microbiota and nutrient digestion in beef cattle. Eight growing beef cattle (live weight 350 ± 25 kg) were allocated in a 2 × 2 crossover design using two levels of dietary CP [111 g/kg dry matter (DM) and 136 g/kg DM] and two levels of TA (0 and 16.9 g/kg DM) as experimental treatments. Each experimental period lasted 19 d, consisting of 14-d adaptation and 5-d sampling. The impacts of dietary CP and TA on ruminal microbiota were analysed using high-throughput sequencing of 16S rRNA gene. Results indicated that no interactions between dietary CP and TA were found on rumen fermentation and nutrient digestibility. Increasing dietary CP level from 111 to 136 g/kg DM increased the ruminal concentrations of ammonia nitrogen (NH₃-N) (p < 0.01) and improved the CP digestibility (p < 0.001). Adding TA at 16.9 g/kg DM inhibited rumen fermentation and decreased the digestibility of dietary CP (p < 0.001), DM (p < 0.05) and organic matter (p < 0.01). Increasing the dietary CP level or adding TA did not affect the relative abundances of the major bacteria Firmicutes and Proteobacteria at the phylum level and Prevotella_1 and Christensenellaceae_R-7_group at the genus level, even though adding TA increased the Shannon index of the ruminal bacterial community. TA was partly hydrolysed to pyrogallol, gallic acid and resorcinol in rumen fluid and the inhibitory effects of TA on rumen fermentation and nutrient digestibility could have been resulted from the TA metabolites including pyrogallol, gallic acid and resorcinol as well as the protein-binding effect.

This study investigated the effects of β-alanine (β-Ala) on rumen fermentation, nutrient digestibility, nitrogen (N) metabolism, plasma biochemical parameters, and rumen bacterial communities in beef steers. Six steers with initial liveweight of 252.8 ± 5.2 kg and 3 treatments of supplementing with 0, 30, or 60 g β-Ala per day to basal diet were allocated in a replicated 3 × 3 Latin square design. Each experimental period was 20 d, of which the first 15 d were for adaptation and the subsequent 5 d were for sampling. The results showed that β-Ala linearly increased the ruminal concentration of microbial crude protein (MCP) (P = 0.005), but it did not affect the ruminal concentrations of ammonia N and total volatile fatty acids (P > 0.10). β-Ala also linearly increased the dry matter (DM) (P = 0.009), organic matter (OM) (P = 0.017) and crude protein (CP) (P = 0.043) digestibility, tended to decrease the acid detergent fiber digestibility (P = 0.077), but it did not affect the neutral detergent fiber digestibility (P = 0.641). β-Ala quadratically increased the relative abundance of ruminal Bacteroidota (P = 0.021) at the phylum level, and increased Prevotella (P = 0.028) and Prevotellaceae_UCG-003 (P = 0.014), and decreased the relative abundance of NK4A214_group (P = 0.009) at the genus level. Feeding steers with β-Ala linearly increased the urinary N (P = 0.006), urea excretions (P = 0.002) and the N retention (P = 0.004), but it did not affect the N utilization efficiency (P = 0.120). β-Ala quadratically increased the plasma concentration of the total antioxidant capacity (P = 0.011) and linearly increased the plasma concentration of insulin-like growth factor-1 (P < 0.001). In summary, dietary supplementation with β-Ala improved the rumen MCP supply and increased the digestibility of DM, OM, CP and the N retention. Further research is necessary to verify the ruminal degradability of β-Ala and to investigate the mechanism of the impact of absorbed β-Ala on the anti-oxidative ability in steers.