Elena Glagoleva

Elena Glagoleva

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Academic Career Length

9Years (2018-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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7 March 2020 in Agrarian science
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IMI. M. Magomedaliyev
I. M. Magomedaliyev
Roman NekrasovRoman Nekrasov
Roman Nekrasov
MCM. G. Chabaev
M. G. Chabaev
VDV. V. Dzhavakhiya
V. V. Dzhavakhiya
EGElena Glagoleva
Elena Glagoleva
MKM. I. Kartashov
M. I. Kartashov
Topicality. To date, an extensive scientific and practical material has been accumulated to study the effectiveness of probiotic feed additives in the feeding of farm animals. Some mechanisms of their action on the animal body have been studied, positive effects have been established, which are expressed in improving the microbial balance of the intestine, increasing the protective properties of the body, and realizing the productive qualities of animals. It is necessary to study the effectiveness of the use of probiotic complexes in comparison with the action of antibiotic-containing drugs.Methodology. It was studied the probiotic complex of Enzymsporin containing a consortium of bacteria of the genus Bacillus (Bacillus subtilis VKM B-2998 D, Bacillus licheniformis VKM B-2999 D, Bacillus subtilis VKM B-3057D) at least 5x109 CFU/g was studied in comparison with antibiotic-containing drug Virginiamycin in feeding fattened young pigs. The studies were carried out on four groups (30 heads in each group) of growing pigs in the conditions of LLC “Bryansk meat processing plant” and in the laboratories of L.K. Ernst Federal Science Center for Animal Husbandry. The duration of the experiment was 113 days.Results. During the studies, the control group of animals received compound feeds without probiotic additives, while the animals of the 2nd and 3rd experimental groups received compound feed with the addition of probiotic complex in an amount of 0.5 and 1.0 kg/t, the 4th experimental group of pigs received compound feed with the addition of antibiotic-containing drug Virginiamycin 250 g/t. By the end of the study, the live weight and average daily increments of experimental animals increased by 5.0; 6.0; 10.02 and 5.8; 6.5; 12.2% (p < 0.001), respectively, compared with the control. Biochemical, morphological, immunological blood indices corresponded to physiological norms, the inclusion in the feed of pigs fed different levels of probiotic complex has led to a significant increase lysozyme, bactericidal and phagocytic activity of blood in animals 2nd and 3rd groups during rearing. Additional profit for the period of research was in the experimental groups of fattened young pigs +705,0; +849,0 and 1440.0 rubles/ head, accordingly, in comparison with the control, which indicates the effective use of not only antibiotic-containing drugs in feeding pigs, but also suggests the effective use of probiotics instead of antibiotic-containing feed preparations.
11 October 2023 in International Journal of Molecular Sciences
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VPVasily Ptushenko
Vasily Ptushenko
DKDmitry D. Knorre
Dmitry D. Knorre
EGElena Glagoleva
Elena Glagoleva
PsbS is one of the key photoprotective proteins, ensuring the tolerance of the photosynthetic apparatus (PSA) of a plant to abrupt changes in irradiance. Being a component of photosystem II, it provides the formation of quenching centers for excited states of chlorophyll in the photosynthetic antenna with an excess of light energy. The signal for “turning on” the photoprotective function of the protein is an excessive decrease in pH in the thylakoid lumen occurring when all the absorbed light energy (stored in the form of transmembrane proton potential) cannot be used for carbon assimilation. Hence, lumen-exposed protonatable amino acid residues that could serve as pH sensors are the essential components of PsbS-dependent photoprotection, and their pKa values are necessary to describe it. Previously, calculations of the lumen-exposed protonatable residue pKa values in PsbS from spinach were described in the literature. However, it has recently become clear that PsbS, although typical of higher plants and charophytes, can also provide photoprotection in green algae. Namely, the stress-induced expression of PsbS was recently shown for two green microalgae species: Chlamydomonas reinhardtii and Lobosphaera incisa. Therefore, we determined the amino acid sequence and modeled the three-dimensional structure of the PsbS from L. incisa, as well as calculated the pKa values of its lumen-exposed protonatable residues. Despite significant differences in amino acid sequence, proteins from L. incisa and Spinacia oleracea have similar three-dimensional structures. Along with the other differences, one of the two pH-sensing glutamates in PsbS from S. oleracea (namely, Glu-173) has no analogue in L. incisa protein. Moreover, there are only four glutamate residues in the lumenal region of the L. incisa protein, while there are eight glutamates in S. oleracea. However, our calculations show that, despite the relative deficiency in protonatable residues, at least two residues of L. incisa PsbS can be considered probable pH sensors: Glu-87 and Lys-196.
27 March 2024 in Proceedings of universities. Applied chemistry and biotechnology
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VYVera Yaderetz
Vera Yaderetz
KNKarpova Nataliya
Karpova Nataliya
EGElena Glagoleva
Elena Glagoleva
KPK. S. Petrova
K. S. Petrova
ASA. S. Shibaeva
A. S. Shibaeva
VDVakhtang Dzhavakhiya
Vakhtang Dzhavakhiya
Carotenoids represent a group of isoprenoid pigments whose high biological activity is not limited to their provitamin properties. Due to their ability to participate in redox reactions, carotenes are increasingly considered as promising compounds in the prevention and correction of cardiovascular and neurodegenerative disorders, as well as in oncology and the treatment of various other diseases. Carotenoids are widely used in the manufacture of food additives and dyes, feed for aquaculture, farm animals and poultry, as well as in so-called nutraceuticals and cosmetics. When formulating optimal feeding rations, vitamin A nutrition is often considered separately due to its vital role in normal growth, development, maintenance and reproduction. The main precursor of vitamin A is β-carotene, which naturally enters the body exclusively via vegetable-based provender. However, since the carotene contained in plant raw materials is an unstable compound, the use of feed additives containing β-carotene becomes relevant. In industry, carotenoids can be produced either by chemical or biological synthesis. However, the majority of carotenoids – 80–90% – are obtained by chemical synthesis. At the same time, public demand for sustainable production dictates the need to find alternative approaches for obtaining this valuable commodity. The article provides an overview of the main biotechnological methods for the production of carotenes using various microorganisms, including microalgae, bacteria and fungi, as well as analysing the effect of culture conditions on the yield of target pigments.
5 December 2018 in bioRxiv
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OKOlga A. Kudryavtseva
Olga A. Kudryavtseva
KSKsenia R. Safina
Ksenia R. Safina
OVOlga A. Vakhrusheva
Olga A. Vakhrusheva
MLMaria Logacheva
Maria Logacheva
APAleksey Penin
Aleksey Penin
AKAlexey S. Kondrashov
Alexey S. Kondrashov
Podospora anserina is a model ascomycetous fungus which shows pronounced phenotypic senescence when grown on solid medium but possesses unlimited lifespan under submerged cultivation. In order to study the genetic aspects of adaptation of P. anserina to submerged cultivation, we initiated a long-term evolution experiment. In the course of the first four years of the experiment, 125 single-nucleotide substitutions and 23 short indels were fixed in eight independently evolving populations. Six proteins that affect fungal growth and development evolved in more than one population; in particular, the G-protein alpha subunit FadA evolved in seven out of eight experimental populations. Parallel evolution at the level of genes and pathways, an excess of nonsense and missense substitutions, and an elevated conservation of proteins and their sites where the changes occurred suggest that many of the observed allele replacements were adaptive and driven by positive selection. Author summary: Living beings adapt to novel conditions that are far from their original environments in different ways. Studying mechanisms of adaptation is crucial for our understanding of evolution. The object of our interest is a multicellular fungus Podospora anserina . This fungus is known for its pronounced senescence and a definite lifespan, but it demonstrates an unlimited lifespan and no signs of senescence when grown under submerged conditions. Soon after transition to submerged cultivation, the rate of growth of P. anserina increases and its pigmentation changes. We wanted to find out whether there are any genetic changes that contribute to adaptation of P. anserina to these novel conditions and initiated a long-term evolutionary experiment on eight independent populations. Over the first four years of the experiment, 148 mutations were fixed in these populations. Many of these mutations lead to inactivation of the part of the developmental pathway in P. anserina , probably reallocating resources to vegetative proliferation in liquid medium. Our observations imply that strong positive selection drives changes in at least some of the affected protein-coding genes. Data Availability: Genome sequence data have been deposited at DDBJ/ENA/GenBank under accessions QHKV00000000 (founder genotype A; version QHKV01000000) and QHKU00000000 (founder genotype B; version QHKU01000000), with the respective BioSample accessions SAMN09270751 and SAMN09270757, under BioProject PRJNA473312. Sequencing data have been deposited at the SRA with accession numbers SRR7233712-SRR7233727, under the same BioProject. Funding: Experimental work and sequencing were supported by the Russian Foundation for Basic Research (grants no. 16-04-01845a and 18-04-01349a). Bioinformatic analysis was supported by the Russian Science Foundation (grant no. 16-14-10173). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
2 April 2026 in Proceedings of universities. Applied chemistry and biotechnology
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ASA. S. Shibaeva
A. S. Shibaeva
EGElena Glagoleva
Elena Glagoleva
VDVakhtang Dzhavakhiya
Vakhtang Dzhavakhiya
TVT. M. Voinova
T. M. Voinova
VYVera Yaderetz
Vera Yaderetz
Повышение активности штамма Amycolatopsis orientalis – продуцента антибиотика эремомицина и изучение его антибактериальной активности
1 December 2023 in Microbiology
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ABAnton Burlaka
Anton Burlaka
EGElena Glagoleva
Elena Glagoleva
NKNataliia Kashko
Nataliia Kashko
DKDmitry A. Knorre
Dmitry A. Knorre
We searched for genes whose deletion affects mitochondrial heteroplasmy in the yeast Saccharomyces cerevisiae. In order to do this, we crossed a yeast knockout collection pool (rho+) with a rho– strain containing a large deletion in its mtDNA. The resulting mixture of cells was plated onto selective media that made it possible to distinguish between diploid cells that had lost and retained wild-type mtDNA as a result of such crossing. From the resulting pools (initial one, cells that lose rho+ mtDNA, and those that retained it), we isolated DNA and determined the proportion of deletion strains using high-throughput sequencing. This made it possible to identify functional groups of genes that reduce the ability of yeast cells to maintain rho+ mtDNA in a state of heteroplasmy with rho– mtDNA.