Hydrogen peroxide (H₂O₂) is an important signaling molecule in plant developmental processes and stress responses. However, whether H₂O₂-mediated signaling crosstalks with plant hormone signaling is largely unclear. Here, we show that H₂O₂ induces the oxidation of the BRASSINAZOLE-RESISTANT1 (BZR1) transcription factor, which functions as a master regulator of brassinosteroid (BR) signaling. Oxidative modification enhances BZR1 transcriptional activity by promoting its interaction with key regulators in the auxin-signaling and light-signaling pathways, including AUXIN RESPONSE FACTOR6 (ARF6) and PHYTOCHROME INTERACTING FACTOR4 (PIF4). Genome-wide analysis shows that H₂O₂-dependent regulation of BZR1 activity plays a major role in modifying gene expression related to several BR-mediated biological processes. Furthermore, we show that the thioredoxin TRXh5 can interact with BZR1 and catalyzes its reduction. We conclude that reversible oxidation of BZR1 connects H₂O₂-mediated and thioredoxin-mediated redox signaling to BR signaling to regulate plant development.

Starch degradation in guard cells is induced by the phytohormone brassinosteroid and the redox signal hydrogen peroxide to promote stomatal opening.

BRs play important roles in the transition from skotomorphogenesis to photomorphogenesis by optimizing the biosynthesis of chlorophyll through the BZR1-PIF4-GRF