Human‐mediated introductions of species may have profound impacts on native ecosystems. One potential impact with largely unforeseen consequences is the potential admixture of introduced with autochthonous species through hybridization. Throughout the world, bumblebees have been deliberately introduced for crop pollination with known negative impacts on native pollinators. Given the likely allochthonous origin of commercial bumblebees used in Portugal (subspecies Bombus terrestris terrestris and B. t. dalmatinus), our aim was to assess their putative introgression with the native Iberian subspecies B. terrestris lusitanicus. We analysed one mitochondrial gene, cytochrome c oxidase subunit I (COX1) and genomic data involving thousands of genome‐wide restriction‐site‐associated DNA markers (RAD‐seq). In the mitochondrial COX1 analyses, we detected one relatively common haplotype in commercial bumblebees, also present in wild samples collected nearby the greenhouses where the commercial hives are used. In the RAD‐seq analysis, we found a clear genetic differentiation between native and commercial lineages. Furthermore, we detected candidate hybrids in the wild, as well as putatively escaped commercial bumblebees, some of which being potentially fertile males. Although we cannot assess directly the fitness effects of introgressed alleles, there is a risk of maladaptive allele introgression to the local bumblebee subspecies, which can negatively impact autochthon populations. One immediate recommendation to farmers is for the proper disposal of hive boxes, after their use in greenhouses, so as to minimize the risk of escapees contaminating native populations. On the other hand, the feasibility of using local subspecies B. t. lusitanicus, preferably with local production, should be evaluated.

Dicyphine mirids are important biological control agents (BCAs) in horticultural crops. Dicyphus cerastii Wagner can be found in protected tomato crops in Portugal, and has been observed feeding on several tomato pests. However, the predation capacity of this species is poorly studied. In order to investigate the predation capacity of D. cerastii, and how it is affected by prey size and mobility, we evaluated the functional response (FR) and predation rate of female predators on different densities of four prey species: Myzus persicae 1st instar nymphs (large mobile prey), Bemisia tabaci 4th instar nymphs, Ephestia kuehniella eggs (large immobile prey) and Tuta absoluta eggs (small immobile prey). Experiments were performed on tomato leaflets in Petri dish arenas for 24 h. Dicyphus cerastii exhibited type II FR for all prey tested. The predator effectively preyed upon all prey, consuming an average of 88.8 B. tabaci nymphs, 134.4 E. kuehniella eggs, 37.3 M. persicae nymphs and 172.3 T. absoluta eggs. Differences in the FR parameters, attack rate and handling time, suggested that prey size and mobility affected predation capacity. Considering the very high predation rates found for all prey species, D. cerastii proved to be an interesting candidate BCA for tomato crops.

Ongoing climate change is expected to cause an increase in temperature and a reduction of precipitation levels in the Mediterranean region, which might cause changes in many species distributions. These effects negatively influence species gene pools, decreasing genetic variability and adaptive potential. Here, we use mitochondrial DNA and RADseq to analyse population genetic structure and genetic diversity of the bumblebee species Bombus terrestris (subspecies Bombus terrestris lusitanicus), in the Iberian Peninsula. Although this subspecies shows a panmictic pattern of population structure across Iberia and beyond, we found differentiation between subspecies B. t. lusitanicus and B. t. africanus, probably caused by the existence of barriers to gene flow between Iberia and North Africa. Furthermore, the results revealed that the Iberian Peninsula harbours a large fraction of B. terrestris intraspecific genetic variation, with the highest number of mitochondrial haplotypes found when compared with any other region in Europe studied so far, suggesting a potential role for the Iberian Peninsula as a glacial refugium. Our findings strengthen the idea that Iberia is a very important source of diversity for the global genetic pool of this species, because rare alleles might play a role in population resilience against human- or climate-mediated changes.