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Vasiliy Chokheli; Antonina Shmaraeva; Viсtoriya Stepanenko; Boris Kozlovsky; Pavel Dmitriev; Vishnu Rajput; Anatoly Azarov; Semyon Bakulin; Tatyana Varduni. Study the informativeness of the marker system in the genetic analysis of coenopopulations of Bellevalia sarmatica (Pall. ex Georgi) Woronow using ISSR primers. Live and Bioabiotic Systems 2020, 1 .
AMA StyleVasiliy Chokheli, Antonina Shmaraeva, Viсtoriya Stepanenko, Boris Kozlovsky, Pavel Dmitriev, Vishnu Rajput, Anatoly Azarov, Semyon Bakulin, Tatyana Varduni. Study the informativeness of the marker system in the genetic analysis of coenopopulations of Bellevalia sarmatica (Pall. ex Georgi) Woronow using ISSR primers. Live and Bioabiotic Systems. 2020; (34):1.
Chicago/Turabian StyleVasiliy Chokheli; Antonina Shmaraeva; Viсtoriya Stepanenko; Boris Kozlovsky; Pavel Dmitriev; Vishnu Rajput; Anatoly Azarov; Semyon Bakulin; Tatyana Varduni. 2020. "Study the informativeness of the marker system in the genetic analysis of coenopopulations of Bellevalia sarmatica (Pall. ex Georgi) Woronow using ISSR primers." Live and Bioabiotic Systems , no. 34: 1.
Vasiliy Chokheli; Boris Kozlovsky; Pavel Dmitriev; Vishnu Rajput; Victoriya Stepanenko; Anatoly Azarov; Semyon Bakulin; Tatyana Varduni. Method for calculating the average expected heterozygosity of cenopopulations of Quercus robur L. (Fagaceae) from different ecotopes. Live and Bioabiotic Systems 2020, 1 .
AMA StyleVasiliy Chokheli, Boris Kozlovsky, Pavel Dmitriev, Vishnu Rajput, Victoriya Stepanenko, Anatoly Azarov, Semyon Bakulin, Tatyana Varduni. Method for calculating the average expected heterozygosity of cenopopulations of Quercus robur L. (Fagaceae) from different ecotopes. Live and Bioabiotic Systems. 2020; (34):1.
Chicago/Turabian StyleVasiliy Chokheli; Boris Kozlovsky; Pavel Dmitriev; Vishnu Rajput; Victoriya Stepanenko; Anatoly Azarov; Semyon Bakulin; Tatyana Varduni. 2020. "Method for calculating the average expected heterozygosity of cenopopulations of Quercus robur L. (Fagaceae) from different ecotopes." Live and Bioabiotic Systems , no. 34: 1.
The current investigation aimed to present an overview of the conservation of biological diversity of rare and endangered plant species. Methods of biodiversity conservation as well as several overview recommendations for the preservation of various rare species have been considered. An overview of the taxa included in the red book has been presented on the example of the Russian Federation. Global and local codes and classifiers of plant rarity were also presented. Future prospects for the conservation of biological diversity and the creation and development of bioresource collections have been considered.
Vasiliy A. Chokheli; Pavel A. Dmitriev; Vishnu D. Rajput; Semyon D. Bakulin; Anatoly S. Azarov; Tatiana V. Varduni; Victoria V. Stepanenko; Sarieh Tarigholizadeh; Rupesh Kumar Singh; Krishan K. Verma; Tatiana M. Minkina. Recent Development in Micropropagation Techniques for Rare Plant Species. Plants 2020, 9, 1733 .
AMA StyleVasiliy A. Chokheli, Pavel A. Dmitriev, Vishnu D. Rajput, Semyon D. Bakulin, Anatoly S. Azarov, Tatiana V. Varduni, Victoria V. Stepanenko, Sarieh Tarigholizadeh, Rupesh Kumar Singh, Krishan K. Verma, Tatiana M. Minkina. Recent Development in Micropropagation Techniques for Rare Plant Species. Plants. 2020; 9 (12):1733.
Chicago/Turabian StyleVasiliy A. Chokheli; Pavel A. Dmitriev; Vishnu D. Rajput; Semyon D. Bakulin; Anatoly S. Azarov; Tatiana V. Varduni; Victoria V. Stepanenko; Sarieh Tarigholizadeh; Rupesh Kumar Singh; Krishan K. Verma; Tatiana M. Minkina. 2020. "Recent Development in Micropropagation Techniques for Rare Plant Species." Plants 9, no. 12: 1733.
Qualitative and quantitative evaluation of the total DNA extracts from the steams tissues of different morphological locations was performed for ecologically and economically important wood tree species pedunculate oak (Quercus robur L.). Generally, the younger plant leaves are known to have a significantly higher content of the undegraded DNA. However, sometimes it is difficult to collect them practically. It follows that qualitative and quantitative DNA isolation may require other organs and tissues of plants, such as the stems. Three types of primers, i.e., nuclear DNA degenerate ISSR-primer UBC 841; chloroplast DNA-primers complementary to the gene fragment (rbcl_a) of a large subunit of 1,5-ribulose-bisphosphate carboxylase/oxygenase (RuBisCO) and mitochondrial DNA-primers targeting the gene encoding ATPase 1 (atp 1) were used for the qualitative evaluation of total DNA extraction. The highest concentration of total DNA was found in the phloem and cambium (35.23 ng/μl for the wood cuts from living plants and 12.62 ng/μl for the wood cuts of dead plants) obtained from the Q. robur L. stems. Successful enzymatic amplification of the isolated DNA was achieved only for the objects from the locations related to the cambium, bast, and sapwoods tissues of stems. Generally, it has been shown that Q. robur L. stem can be used for the obtaining of an acceptable concentrations of a good quality DNA. The results of the current study are presented for the first time to make the practical recommendations for sampling the tissues from a living woody tree with the subsequent PCR analysis.
V.A. Chokheli; Igor Kornienko; B.L. Kozlovsky; Vishnu Rajput; T.V. Varduny; V.S. Lysenko. A comparative evaluation of genetic material from Quercus robur L. stem tissues of different age and living states. Biocatalysis and Agricultural Biotechnology 2020, 23, 101497 .
AMA StyleV.A. Chokheli, Igor Kornienko, B.L. Kozlovsky, Vishnu Rajput, T.V. Varduny, V.S. Lysenko. A comparative evaluation of genetic material from Quercus robur L. stem tissues of different age and living states. Biocatalysis and Agricultural Biotechnology. 2020; 23 ():101497.
Chicago/Turabian StyleV.A. Chokheli; Igor Kornienko; B.L. Kozlovsky; Vishnu Rajput; T.V. Varduny; V.S. Lysenko. 2020. "A comparative evaluation of genetic material from Quercus robur L. stem tissues of different age and living states." Biocatalysis and Agricultural Biotechnology 23, no. : 101497.
The increased application and releases of nanoparticles in the environments have drawn consideration of scientific attention. The potential release of nanoparticles could increase concentration in the atmosphere which may impact on plants and other biota of soil systems. The present chapter focused on fate and toxicity of metallic nanoparticles on terrestrial plants, biotransformation mechanism, uptake, translocation, cellular, sub-cellular toxicity, detection techniques and future concerns. The main focus of the chapter is on copper and zinc oxide nanoparticles, as they are essential elements for plants growth. It is required to understand nanoparticles ecological threats before the applications and releases in the environment.
Vishnu D. Rajput; Tatiana Minkina; Svetlana Sushkova; Vasiliy Chokheli; Mikhail Soldatov. Toxicity assessment of metal oxide nanoparticles on terrestrial plants. Engineered Nanomaterials and Phytonanotechnology: Challenges for Plant Sustainability 2019, 87, 189 -207.
AMA StyleVishnu D. Rajput, Tatiana Minkina, Svetlana Sushkova, Vasiliy Chokheli, Mikhail Soldatov. Toxicity assessment of metal oxide nanoparticles on terrestrial plants. Engineered Nanomaterials and Phytonanotechnology: Challenges for Plant Sustainability. 2019; 87 ():189-207.
Chicago/Turabian StyleVishnu D. Rajput; Tatiana Minkina; Svetlana Sushkova; Vasiliy Chokheli; Mikhail Soldatov. 2019. "Toxicity assessment of metal oxide nanoparticles on terrestrial plants." Engineered Nanomaterials and Phytonanotechnology: Challenges for Plant Sustainability 87, no. : 189-207.
S.I. Kolesnikov; T.V. Varduny; V.S. Lysenko; O.A. Kapralova; Vasiliy Chokheli; M.M. Sereda; P.A. Dmitriev; V.M. Varduny. Effect of nano- and crystalline metal oxides on growth, gene- and cytotoxicity of plants in vitro and ex vitro. Turczaninowia 2018, 21, 207 -214.
AMA StyleS.I. Kolesnikov, T.V. Varduny, V.S. Lysenko, O.A. Kapralova, Vasiliy Chokheli, M.M. Sereda, P.A. Dmitriev, V.M. Varduny. Effect of nano- and crystalline metal oxides on growth, gene- and cytotoxicity of plants in vitro and ex vitro. Turczaninowia. 2018; 21 (4):207-214.
Chicago/Turabian StyleS.I. Kolesnikov; T.V. Varduny; V.S. Lysenko; O.A. Kapralova; Vasiliy Chokheli; M.M. Sereda; P.A. Dmitriev; V.M. Varduny. 2018. "Effect of nano- and crystalline metal oxides on growth, gene- and cytotoxicity of plants in vitro and ex vitro." Turczaninowia 21, no. 4: 207-214.
Vasiliy Chokheli; D.I. Kagan; T.V. Varduny; B.L. Kozlovsky; M.M. Sereda; O.A. Kapralova; P.A. Dmitriev; V.E. Padutov. Ecological and genetic differentiation of populations of Quercus robur L. in the Rostov Region with the use of ISSR-markers. Turczaninowia 2018, 21, 161 -167.
AMA StyleVasiliy Chokheli, D.I. Kagan, T.V. Varduny, B.L. Kozlovsky, M.M. Sereda, O.A. Kapralova, P.A. Dmitriev, V.E. Padutov. Ecological and genetic differentiation of populations of Quercus robur L. in the Rostov Region with the use of ISSR-markers. Turczaninowia. 2018; 21 (4):161-167.
Chicago/Turabian StyleVasiliy Chokheli; D.I. Kagan; T.V. Varduny; B.L. Kozlovsky; M.M. Sereda; O.A. Kapralova; P.A. Dmitriev; V.E. Padutov. 2018. "Ecological and genetic differentiation of populations of Quercus robur L. in the Rostov Region with the use of ISSR-markers." Turczaninowia 21, no. 4: 161-167.
Objective: Staurogyn (Staurogyne repens (Nees) Kuntze) is a perennial herb, widely used in aquarium landscape design. Representatives of the Staurogyne species are sources of unique glycosides in their chemical composition, which are taste stimulators. However, a method of the tissue culture of S. repens was not described in the literature until now. In this study, such a method is proposed. Materials and Methods: Node bines with axillary buds were taken as explants. Surface sterilization with mercuric chloride (HgCl2) was found to be the most effective (60% survivors of sterile explants). Solid and liquid media contained 3% sucrose and various combinations of plant hormones: Benzyladenine (BA), thidiazuron (TDZ) and indole-3-acetic acid (IAA) were applied. Results: It was shown that the intensive multiplication of shoots can be induced using a liquid Murashige-Skoog (MS) medium with the following combination of phytohormones: Benzyl adenine (2 mg L–1) and indole-3-acetic acid (0.5 mg L–1). The MS concentration of 0.2 mg L–1 was the most effective for the rhyzogenesis. The adaptation of plants was successful enough under conditions of regular liquid media changeout and under maintaining overwater air humidity of 90% when the plants were grown in emergent conditions. Conclusion: The developed tissue culture method for micropropagation of aquatic plant Staurogyne repens can used be for commercial purposes.
M.M. Sereda; E.V. Lutsenko; Vasiliy Chokheli; A.V. Vereschagina; K.Y. Rachkovskaya; V.S. Lysenko; T.V. Varduny. A Method for Microclonal Propagation of Staurogyne repens in Tissue Culture. Journal of Plant Sciences 2016, 12, 17 -21.
AMA StyleM.M. Sereda, E.V. Lutsenko, Vasiliy Chokheli, A.V. Vereschagina, K.Y. Rachkovskaya, V.S. Lysenko, T.V. Varduny. A Method for Microclonal Propagation of Staurogyne repens in Tissue Culture. Journal of Plant Sciences. 2016; 12 (1):17-21.
Chicago/Turabian StyleM.M. Sereda; E.V. Lutsenko; Vasiliy Chokheli; A.V. Vereschagina; K.Y. Rachkovskaya; V.S. Lysenko; T.V. Varduny. 2016. "A Method for Microclonal Propagation of Staurogyne repens in Tissue Culture." Journal of Plant Sciences 12, no. 1: 17-21.
Quercus robur L. is a valuable wood species having long ontogeny and promising to create long-living artificial plantings of recreational and ameliorative purposes in the steppes zone of Russia and other countries. In this work we have performed the genotyping of varieties of Quercus robur L. obtained from collection of Botanical Garden of Southern Federal University using intersimple sequence repeat (ISSR) molecular markers. The most polymorphic ISSR-marker (GA) 8YC was found in the collection. The polymorphic DNA markers identified in the present study can be used for the future breeding works to obtain valuable genotypes of Quercus genus. In addition we have performed DNA fingerprinting of the prospective sample of the variety Q. robur var. tardiflora Czern.
Vasiliy Chokheli; Boris Kozlovsky; Mikhail Sereda; Vladimir Lysenko; Igor Fesenko; Tatiana Varduny; Olga Kapralova; Elena Bondarenko. Preliminary Comparative Analysis of Phenological Varieties of Quercus robur by ISSR-Markers. Journal of Botany 2016, 2016, 1 -7.
AMA StyleVasiliy Chokheli, Boris Kozlovsky, Mikhail Sereda, Vladimir Lysenko, Igor Fesenko, Tatiana Varduny, Olga Kapralova, Elena Bondarenko. Preliminary Comparative Analysis of Phenological Varieties of Quercus robur by ISSR-Markers. Journal of Botany. 2016; 2016 ():1-7.
Chicago/Turabian StyleVasiliy Chokheli; Boris Kozlovsky; Mikhail Sereda; Vladimir Lysenko; Igor Fesenko; Tatiana Varduny; Olga Kapralova; Elena Bondarenko. 2016. "Preliminary Comparative Analysis of Phenological Varieties of Quercus robur by ISSR-Markers." Journal of Botany 2016, no. : 1-7.
Vladimir Lysenko; T. V. Varduny; E. I. Simonovich; O. I. Chugueva; Vasiliy Chokheli; Mikhail Sereda; S. N. Gorbov; V. P. Krasnov; E. K. Tarasov; I. Y. Sherstneva; M. Yu Kozlova. Far-Red Spectrum of Second Emerson Effect: A Study Using Dual-Wavelength Pulse Amplitude Modulation Fluorometry. American Journal of Biochemistry and Biotechnology 2014, 10, 234 -240.
AMA StyleVladimir Lysenko, T. V. Varduny, E. I. Simonovich, O. I. Chugueva, Vasiliy Chokheli, Mikhail Sereda, S. N. Gorbov, V. P. Krasnov, E. K. Tarasov, I. Y. Sherstneva, M. Yu Kozlova. Far-Red Spectrum of Second Emerson Effect: A Study Using Dual-Wavelength Pulse Amplitude Modulation Fluorometry. American Journal of Biochemistry and Biotechnology. 2014; 10 (4):234-240.
Chicago/Turabian StyleVladimir Lysenko; T. V. Varduny; E. I. Simonovich; O. I. Chugueva; Vasiliy Chokheli; Mikhail Sereda; S. N. Gorbov; V. P. Krasnov; E. K. Tarasov; I. Y. Sherstneva; M. Yu Kozlova. 2014. "Far-Red Spectrum of Second Emerson Effect: A Study Using Dual-Wavelength Pulse Amplitude Modulation Fluorometry." American Journal of Biochemistry and Biotechnology 10, no. 4: 234-240.