Bovine respiratory disease (BRD) is one of the most common calfhood diseases in dairy cattle. Unfortunately, published data on the short- and long-term effects of calfhood BRD on health and performance are scarce and, when available, often conflicting and uncertain. The objective of this study was therefore to review the scientific literature on the effects of calfhood BRD on health and performance of dairy cattle and summarize the research findings using a meta-analysis approach. The systematic review and meta-analysis were performed following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. PubMed and CAB Abstract databases were screened for relevant studies that were published in English (until February 3, 2020). Only studies reporting naturally occurring BRD cases from birth to 12 mo of age in dairy calves in Europe and North America were considered eligible. Both observational studies and randomized controlled trials were included. Random-effect meta-analysis models were used to quantify the effect of BRD on outcomes for which at least 5 studies reported a numeric estimation of the effect of BRD. From an initial pool of 525 references, 27 full-text articles with original data were included in the manuscript. Meta-analysis models were prepared for 4 outcomes: odds of mortality (n = 7 studies), odds of herd removal before first calving (n = 5 studies), average daily gain (n = 10 studies), and milk production during first lactation (n = 5 studies). In these models, heifers diagnosed with BRD during calfhood had 2.85 times higher odds of dying (95% confidence interval: 1.22 to 6.69) and 2.30 times higher odds of herd removal (i.e., dead, culled, or sold) before the first calving (95% confidence interval: 1.75 to 3.03) compared with heifers not diagnosed with this condition. Heifers experiencing calfhood BRD also had an average daily gain reduced by 0.067 kg/d (95% confidence interval: -0.099 to -0.034) and they produced 121.2 kg (95% confidence interval: -184.9 to -57.5) less milk during their first lactation. Other health and performance outcomes, such as age at first calving and odds of not finishing the first lactation, were also investigated but not summarized using a meta-analysis approach. Pooled estimates obtained in the present study may provide more accurate estimates of the overall economic losses associated with calfhood BRD in dairy cattle.

Background: Bacterial bronchopneumonia (BP) is the leading cause of morbidity and mortality in cattle. The nasopharynx is generally accepted as the primary source of pathogenic bacteria that cause BP. However, it has recently been shown in humans that the oropharynx may act as the primary reservoir for pathogens that reach the lung. The objective was therefore to describe the bacterial microbiota present along the entire cattle respiratory tract to determine which upper respiratory tract (URT) niches may contribute the most to the composition of the lung microbiota.Methods: Seventeen upper and lower respiratory tract locations were sampled from 15 healthy feedlot steer calves. Samples were collected using a combination of swabs, protected specimen brushes, and saline washes. DNA was extracted from each sample and the 16S rRNA gene (V3-V4) was sequenced. Community composition, alpha-diversity, and beta-diversity were compared among sampling locations.Results: Microbiota composition differed across sampling locations, with physiologically and anatomically distinct locations showing different relative abundances of 1137 observed sequence variants (SVs). An analysis of similarities showed that the lung was more similar to the nasopharynx (R-statistic = 0.091) than it was to the oropharynx (R-statistic = 0.709) or any other URT sampling location. Five distinct metacommunities were identified across all samples after clustering at the genus level using Dirichlet multinomial mixtures. This included a metacommunity found primarily in the lung and nasopharynx that was dominated byMycoplasma. Further clustering at the SV level showed a shared metacommunity between the lung and nasopharynx that was dominated byMycoplasma dispar. Other metacommunities found in the nostrils, tonsils, and oral microbiotas were dominated byMoraxella,Fusobacterium, andStreptococcus, respectively.Conclusions: The nasopharyngeal bacterial microbiota is most similar to the lung bacterial microbiota in healthy cattle and therefore may serve as the primary source of bacteria to the lung. This finding indicates that the nasopharynx is likely the most important location that should be targeted when doing bovine respiratory microbiota research.