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Prof. Dr. Hélène Thomas-Guyon
LIENSs, CNRS – La Rochelle University, 17000 La Rochelle, France

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0 Biomarkers
0 DNA damage
0 Immunology
0 Metabolism
0 Oxidative Stress

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Oxidative Stress
Sentinel species
Metabolism

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Journal article
Published: 01 March 2020 in Chemosphere
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The development of human activities on French Atlantic coastlines (La Rochelle) lead to chronic pollution of the environment by organic (pesticides, hydrocarbons, agrochemicals) and inorganic (heavy metals) contaminants. These past years, several regulations have been implemented to preserve coastal environments. The purpose of this study was to perform biomonitoring of bivalve species using an outdoor caging technique. The goal of our work was to assess the impact of harbour's trace elements on the state of health of the marine bivalve Mimachlamys varia. First, various molecular defence biomarkers were measured: SOD (oxidative stress), GST (detoxification process), MDA (lipid peroxidation), and Laccase (immune reaction). Thus, in April 2016, scallops were collected at three caging sites, which differ by their levels of pollution, after transplantation into port areas (fairing, rainwater) and a control site (marsh). Bivalve samples were taken at three sampling dates (D0, D07, D21). Biomarker assays were performed in the digestive glands due to their bioaccumulation properties. The second aim was to explore the impacts of inorganic pollutants placed in environmental harbour's sites. After 21 days, the biomarker response of transplanted bivalves revealed a SOD decrease, Laccase and GST stimulations, higher concentrations in Cu, Fe, As, Co, Mn, Zn, Sn and no significant variation of MDA concentration. Our ecological relevance of biomarker approaches opens interesting perspectives to identify M. varia such as a pertinent marine sentinel species. The several selected biomarkers determined could confirm their ability to appraise the water quality of hydro-systems located in French coastlines, such as port areas.

ACS Style

Marine Breitwieser; Marine Barbarin; Christine Plumejeaud-Perreau; Emmanuel Dubillot; Thierry Guyot; Valérie Huet; Carine Churlaud; Thibaut Coulombier; Isabelle Brenon; Denis Fichet; Nathalie Imbert; Hélène Thomas. Biomonitoring of Mimachlamys varia transplanted to areas impacted by human activities (La Rochelle Marina, France). Chemosphere 2020, 243, 125199 .

AMA Style

Marine Breitwieser, Marine Barbarin, Christine Plumejeaud-Perreau, Emmanuel Dubillot, Thierry Guyot, Valérie Huet, Carine Churlaud, Thibaut Coulombier, Isabelle Brenon, Denis Fichet, Nathalie Imbert, Hélène Thomas. Biomonitoring of Mimachlamys varia transplanted to areas impacted by human activities (La Rochelle Marina, France). Chemosphere. 2020; 243 ():125199.

Chicago/Turabian Style

Marine Breitwieser; Marine Barbarin; Christine Plumejeaud-Perreau; Emmanuel Dubillot; Thierry Guyot; Valérie Huet; Carine Churlaud; Thibaut Coulombier; Isabelle Brenon; Denis Fichet; Nathalie Imbert; Hélène Thomas. 2020. "Biomonitoring of Mimachlamys varia transplanted to areas impacted by human activities (La Rochelle Marina, France)." Chemosphere 243, no. : 125199.

Journal article
Published: 01 May 2018 in Ecotoxicology and Environmental Safety
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ACS Style

Marine Breitwieser; Hélène Thomas-Guyon; Valérie Huet; Kjetil Sagerup; Perrine Geraudie. Spatial and temporal impacts of the Skjervøy harbour diesel spill on native population of blue mussels: A sub-Arctic case study. Ecotoxicology and Environmental Safety 2018, 153, 168 -174.

AMA Style

Marine Breitwieser, Hélène Thomas-Guyon, Valérie Huet, Kjetil Sagerup, Perrine Geraudie. Spatial and temporal impacts of the Skjervøy harbour diesel spill on native population of blue mussels: A sub-Arctic case study. Ecotoxicology and Environmental Safety. 2018; 153 ():168-174.

Chicago/Turabian Style

Marine Breitwieser; Hélène Thomas-Guyon; Valérie Huet; Kjetil Sagerup; Perrine Geraudie. 2018. "Spatial and temporal impacts of the Skjervøy harbour diesel spill on native population of blue mussels: A sub-Arctic case study." Ecotoxicology and Environmental Safety 153, no. : 168-174.

Book chapter
Published: 01 January 2018 in Oil Spill Studies
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ACS Style

Marine Breitwieser; Rose Campbell; Matthieu Dussauze; Angélique Fontanaud; Stéphane Le Floch; Florian Lelchat; Thomas Milinkovitch; Frédéric Muttin; Yann Rabuteau; Hélène Thomas-Guyon. List of Authors. Oil Spill Studies 2018, 133 .

AMA Style

Marine Breitwieser, Rose Campbell, Matthieu Dussauze, Angélique Fontanaud, Stéphane Le Floch, Florian Lelchat, Thomas Milinkovitch, Frédéric Muttin, Yann Rabuteau, Hélène Thomas-Guyon. List of Authors. Oil Spill Studies. 2018; ():133.

Chicago/Turabian Style

Marine Breitwieser; Rose Campbell; Matthieu Dussauze; Angélique Fontanaud; Stéphane Le Floch; Florian Lelchat; Thomas Milinkovitch; Frédéric Muttin; Yann Rabuteau; Hélène Thomas-Guyon. 2018. "List of Authors." Oil Spill Studies , no. : 133.

Book chapter
Published: 01 January 2018 in Oil Spill Studies
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ACS Style

Thomas Milinkovitch; Stéphane Le Floch; Hélène Thomas-Guyon. Oil Spill Dispersant Use: Toxicity on Marine Teleost Fish. Oil Spill Studies 2018, 71 -82.

AMA Style

Thomas Milinkovitch, Stéphane Le Floch, Hélène Thomas-Guyon. Oil Spill Dispersant Use: Toxicity on Marine Teleost Fish. Oil Spill Studies. 2018; ():71-82.

Chicago/Turabian Style

Thomas Milinkovitch; Stéphane Le Floch; Hélène Thomas-Guyon. 2018. "Oil Spill Dispersant Use: Toxicity on Marine Teleost Fish." Oil Spill Studies , no. : 71-82.

Article
Published: 02 November 2017 in Fish Physiology and Biochemistry
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In ectotherms, temperature modulates oxidative stress, a key driver of aging. However, the effects of temperature on oxidative stress have not been investigated at several life stages of an ectotherm. In order to improve understanding of aging processes, we conducted a cross-sectional study in short-lived ectotherm vertebrates, the killifish Nothobranchius furzeri. Malondialdehyde contents were assessed at three stages of life, in the liver and muscles of fish acclimatized to optimal or sub-optimal temperatures during all their life cycle. In accordance with the “free-radical theory,” our results highlighted an increase in lipid peroxidation in senescent organisms. In the liver, this lipid peroxidation increase was more intense in senescent fish acclimatized to sub-optimal temperature than in fish acclimatized to their optimal temperature.

ACS Style

Thomas Milinkovitch; Christel Lefrançois; Marie Durollet; Hélène Thomas-Guyon. Influence of temperature on age-related lipid peroxidation in a short-lived vertebrate (Nothobranchius furzeri). Fish Physiology and Biochemistry 2017, 44, 343 -347.

AMA Style

Thomas Milinkovitch, Christel Lefrançois, Marie Durollet, Hélène Thomas-Guyon. Influence of temperature on age-related lipid peroxidation in a short-lived vertebrate (Nothobranchius furzeri). Fish Physiology and Biochemistry. 2017; 44 (1):343-347.

Chicago/Turabian Style

Thomas Milinkovitch; Christel Lefrançois; Marie Durollet; Hélène Thomas-Guyon. 2017. "Influence of temperature on age-related lipid peroxidation in a short-lived vertebrate (Nothobranchius furzeri)." Fish Physiology and Biochemistry 44, no. 1: 343-347.

Journal article
Published: 01 November 2017 in Science of The Total Environment
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The impact of dispersed crude oil and dispersant on adult Pacific oysters, Crassostrea gigas, was evaluated through an integrative biomarker approach including (1) biochemical (plasma catecholase- and laccase-type phenoloxidase and superoxide dismutase), (2) histological (digestive cell lysosomal responses, digestive gland histopathology) and (3) physiological (flesh condition index) endpoints in the haemolymph and digestive gland. Adult oysters were exposed to non-contaminated water (control), chemically-dispersed oil (Brut Arabian Light), mechanically-dispersed oil and dispersant (FINASOL®) alone for 2 days, and further depurated in non-contaminated water for 4 weeks. After exposure to chemically and mechanically dispersed oil oysters exhibited induction of plasma laccase-type phenoloxidase and superoxide dismutase activities, enlargement of digestive cell lysosomes, lipofuscin accumulation, reduced neutral lipid content and atrophy of digestive gland diverticula; more markedly on exposure to chemically dispersed oil. From the studied biomarkers, only lysosomal biomarkers were significantly affected after exposure to the dispersant alone. This included lysosomal enlargement, neutral lipid depletion and lipofuscin accumulation in the digestive gland epithelium. A recovery of plasma enzyme activities was observed after 4 weeks of depuration. The integrative biological response index indicated that chemically dispersed oil caused significantly higher stress to C. gigas than the mechanically-dispersed one or the dispersant alone; nevertheless, the response seems to be reversible after depuration.

ACS Style

Andrea Luna-Acosta; Paco Bustamante; Hélène Thomas-Guyon; Beñat Zaldibar; Urtzi Izagirre; Ionan Marigómez. Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas. Science of The Total Environment 2017, 598, 713 -721.

AMA Style

Andrea Luna-Acosta, Paco Bustamante, Hélène Thomas-Guyon, Beñat Zaldibar, Urtzi Izagirre, Ionan Marigómez. Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas. Science of The Total Environment. 2017; 598 ():713-721.

Chicago/Turabian Style

Andrea Luna-Acosta; Paco Bustamante; Hélène Thomas-Guyon; Beñat Zaldibar; Urtzi Izagirre; Ionan Marigómez. 2017. "Integrative biomarker assessment of the effects of chemically and mechanically dispersed crude oil in Pacific oysters, Crassostrea gigas." Science of The Total Environment 598, no. : 713-721.

Comparative study
Published: 01 September 2017 in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
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Evaluating diffuse sediment contamination in the environment is a major concern with the aim of reaching a good chemical and ecological state of the littoral zone. In this study the risks of chronic chemical contamination and consequences in the bivalves Crassostrea gigas, Mytilus sp. and Mimachlamys varia were evaluated in coastal environments. The objective here was to understand the anthropological phenomena that affect the functioning of the marina of La Rochelle (semi-closed environment). Harbours seeking ecomanagement accreditations (such as the international reference ISO 14001) constitute zones of interest to implement biomonitoring studies. The biological effects of chemical pollution in the Marina of La Rochelle were studied to develop a multi-biomarker biomonitoring approach on specific marine species of this site. Moreover, a genetic (DNA barcoding) approach was applied to validate the species identity of collected bivalves. Of the three species tested the scallop, M. varia, was the most sensitive to metal exposure.

ACS Style

Marine Breitwieser; Amélia Viricel; Carine Churlaud; Benoit Guillot; Elie Martin; Pierre-Louis Stenger; Valérie Huet; Angélique Fontanaud; Hélène Thomas-Guyon. First data on three bivalve species exposed to an intra-harbour polymetallic contamination (La Rochelle, France). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2017, 199, 28 -37.

AMA Style

Marine Breitwieser, Amélia Viricel, Carine Churlaud, Benoit Guillot, Elie Martin, Pierre-Louis Stenger, Valérie Huet, Angélique Fontanaud, Hélène Thomas-Guyon. First data on three bivalve species exposed to an intra-harbour polymetallic contamination (La Rochelle, France). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2017; 199 ():28-37.

Chicago/Turabian Style

Marine Breitwieser; Amélia Viricel; Carine Churlaud; Benoit Guillot; Elie Martin; Pierre-Louis Stenger; Valérie Huet; Angélique Fontanaud; Hélène Thomas-Guyon. 2017. "First data on three bivalve species exposed to an intra-harbour polymetallic contamination (La Rochelle, France)." Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 199, no. : 28-37.

Journal article
Published: 01 April 2017 in Aquatic Toxicology
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Metallothioneins and vitellogenins are low molecular weight proteins that have been used widely in environmental monitoring as biomarkers of exposure and damage to metals and estrogenic compounds, respectively. In the present study, the responses of metallothionein and vitellogenin tissue concentrations were measured following acute (96h) aqueous exposures to cadmium in Saccostrea sp., a tropical cup oyster native to the Western Pacific Ocean that has recently established itself in the Caribbean Sea. Adult oysters (1.5-5.0cm shell length) collected from the municipal marina of Santa Marta, Colombia (Caribbean Sea) and acclimated for 5days in the laboratory, were exposed to Cd at five concentrations (0, 1, 10, 100 and 1000μg/L) and their tissues (gills, digestive gland and adductor muscle) were analyzed in pools of 5 individuals (3 replicates per concentration). Metallothioneins in digestive glands of oysters exposed to Cd concentrations≥100μg/L showed a significant increase, from 8.0 to 14.8μg MT/mg total protein, whereas metallothionein concentrations in gills increased to lesser extent, and no differences were observed in adductor muscle. Metallothionein concentrations in digestive gland and gills correlated directly with whole soft tissue Cd concentrations (ranging from 2 to 297μg/g dw Cd). Vitellogenin in homogenates of oyster gonad tissue, after 96h of exposure to 1000μg/L Cd, were significantly lower (0.04mg P/g gonad) compared to control oysters (0.68mg P/g gonad), suggestive of an anti-estrogenic effect of Cd at high concentrations, whereas no significant changes in vitellogenin concentrations were observed at intermediate Cd exposure concentrations. This study confirms acute responses of metallothionein and vitellogenin concentrations in tissues of Saccostrea sp. exposed to high concentrations of cadmium (Cd≥100μg/L, 96h). The present results are first step towards validating the use of these two proteins as biomarkers of metal exposure in this species.

ACS Style

Angela Margarita Moncaleano Niño; Sergio A. Barrios-Latorre; Javier F. Poloche-Hernández; Vanessa Becquet; Valérie Huet; Luisa Villamil; Hélène Thomas-Guyon; Michael J. Ahrens; Andrea Luna-Acosta. Alterations of tissue metallothionein and vitellogenin concentrations in tropical cup oysters (Saccostrea sp.) following short-term (96 h) exposure to cadmium. Aquatic Toxicology 2017, 185, 160 -170.

AMA Style

Angela Margarita Moncaleano Niño, Sergio A. Barrios-Latorre, Javier F. Poloche-Hernández, Vanessa Becquet, Valérie Huet, Luisa Villamil, Hélène Thomas-Guyon, Michael J. Ahrens, Andrea Luna-Acosta. Alterations of tissue metallothionein and vitellogenin concentrations in tropical cup oysters (Saccostrea sp.) following short-term (96 h) exposure to cadmium. Aquatic Toxicology. 2017; 185 ():160-170.

Chicago/Turabian Style

Angela Margarita Moncaleano Niño; Sergio A. Barrios-Latorre; Javier F. Poloche-Hernández; Vanessa Becquet; Valérie Huet; Luisa Villamil; Hélène Thomas-Guyon; Michael J. Ahrens; Andrea Luna-Acosta. 2017. "Alterations of tissue metallothionein and vitellogenin concentrations in tropical cup oysters (Saccostrea sp.) following short-term (96 h) exposure to cadmium." Aquatic Toxicology 185, no. : 160-170.

Journal article
Published: 12 May 2016 in Environmental Science and Pollution Research
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The decrease of ice cover in polar areas is expected to lead to an increase in ship traffic. In this context, the risk associated with exposure of the aquatic environment to oil-related chemicals from spills and/or accidental fuel discharges from ships will increase in the near future, potentially causing negative impacts on sensitive Arctic species. This study investigated for the first time the biomarker responses of the Icelandic scallops (Chlamys islandica) to marine diesel exposure. Antioxidant response, neurotoxic effect, gonad maturation, and lipid content were assessed in male and female scallops, Chlamys islandica after a 7-day exposure to marine diesel. At the end of exposure, results showed an inhibition of acetylcholinesterase activity in Icelandic scallops exposed to high concentration of diesel. At the end of exposure, results showed an increase in naphthalene residues in hemolymph, together with an inhibition of AChE activity in Icelandic scallops exposed to high concentration of diesel. In addition, alteration of the escape capacity was observed suggesting effects on behavior responses. Overall, this study contributes to the better knowledge of physiological effects of marine diesel in Arctic marine organisms.

ACS Style

Perrine Geraudie; Renée Bakkemo; Thomas Milinkovitch; Helene Thomas-Guyon. First evidence of marine diesel effects on biomarker responses in the Icelandic scallops, Chlamys islandica. Environmental Science and Pollution Research 2016, 23, 16504 -16512.

AMA Style

Perrine Geraudie, Renée Bakkemo, Thomas Milinkovitch, Helene Thomas-Guyon. First evidence of marine diesel effects on biomarker responses in the Icelandic scallops, Chlamys islandica. Environmental Science and Pollution Research. 2016; 23 (16):16504-16512.

Chicago/Turabian Style

Perrine Geraudie; Renée Bakkemo; Thomas Milinkovitch; Helene Thomas-Guyon. 2016. "First evidence of marine diesel effects on biomarker responses in the Icelandic scallops, Chlamys islandica." Environmental Science and Pollution Research 23, no. 16: 16504-16512.

Research article
Published: 03 March 2016 in PLoS ONE
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Understanding the effects of chronic chemical contamination on natural populations of marine organisms is complex due to the combined effects of different types of pollutants and environmental parameters that can modulate the physiological responses to stress. Here, we present the effects of a chronic contamination in a marine bivalve by combining multiple approaches that provide information on individual and population health. We sampled variegated scallops (Mimachlamys varia) at sites characterized by different contaminants and contamination levels to study the short and long-term (intergenerational) responses of this species to physiological stress. We used biomarkers (SOD, MDA, GST, laccase, citrate synthase and phosphatases) as indicators of oxidative stress, immune system alteration, mitochondrial respiration and general metabolism, and measured population genetic diversity at each site. In parallel, concentration of 14 trace metals and 45 organic contaminants (PAHs, PCBs, pesticides) in tissues were measured. Scallops were collected outside and during their reproductive season to investigate temporal variability in contaminant and biomarker levels. Our analyses revealed that the levels of two biomarkers (Laccase-type phenoloxidase and malondialdehyde) were significantly correlated with Cd concentration. Additionally, we observed significant seasonal differences for four of the five biomarkers, which is likely due to the scallop reproductive status at time of sampling. As a source of concern, a location that was identified as a reference site on the basis of inorganic contaminant levels presented the same level of some persistent organic pollutants (DDT and its metabolites) than more impacted sites. Finally, potential long-term effects of heavy metal contamination were observed for variegated scallops as genetic diversity was depressed in the most polluted sites.

ACS Style

Marine Breitwieser; Amélia Viricel; Marianne Graber; Laurence Murillo; Vanessa Becquet; Carine Churlaud; Ingrid Arnaudin; Valérie Huet; Camille Lacroix; Eric Pante; Stéphane Le Floch; Hélène Thomas-Guyon. Short-Term and Long-Term Biological Effects of Chronic Chemical Contamination on Natural Populations of a Marine Bivalve. PLoS ONE 2016, 11, e0150184 .

AMA Style

Marine Breitwieser, Amélia Viricel, Marianne Graber, Laurence Murillo, Vanessa Becquet, Carine Churlaud, Ingrid Arnaudin, Valérie Huet, Camille Lacroix, Eric Pante, Stéphane Le Floch, Hélène Thomas-Guyon. Short-Term and Long-Term Biological Effects of Chronic Chemical Contamination on Natural Populations of a Marine Bivalve. PLoS ONE. 2016; 11 (3):e0150184.

Chicago/Turabian Style

Marine Breitwieser; Amélia Viricel; Marianne Graber; Laurence Murillo; Vanessa Becquet; Carine Churlaud; Ingrid Arnaudin; Valérie Huet; Camille Lacroix; Eric Pante; Stéphane Le Floch; Hélène Thomas-Guyon. 2016. "Short-Term and Long-Term Biological Effects of Chronic Chemical Contamination on Natural Populations of a Marine Bivalve." PLoS ONE 11, no. 3: e0150184.

Journal article
Published: 21 October 2015 in Environmental Science and Pollution Research
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The decrease of ice cover in the Arctic will lead to an increase of ship traffic in the upcoming decades. Consequently, oil pollution is expected. In this context, the goals of this study were to evaluate the biological impact of marine diesel contamination and, on this basis, to determine analytical tools of interest (biomarkers) for future biomonitoring of diesel spills. Using a 7-day contamination protocol, this study investigated biochemical modulations in the digestive gland of the Iceland scallop (Chlamys islandica). Incorporation of contaminants was verified assessing haemolymph metabolites. Results showed a response of glutathione-S-transferase to contamination suggesting detoxification processes and the suitability of such a tool for diesel spill biomonitoring. The lack of modulation of superoxide dismutase activity and lipid peroxidation suggests no oxidative stress and the unsuitability of these molecular tools for biomonitoring.

ACS Style

Thomas Milinkovitch; Perrine Geraudie; Lionel Camus; Valérie Huet; Hélène Thomas-Guyon. Biomarker modulation associated with marine diesel contamination in the Iceland scallop (Chlamys islandica). Environmental Science and Pollution Research 2015, 22, 19292 -19296.

AMA Style

Thomas Milinkovitch, Perrine Geraudie, Lionel Camus, Valérie Huet, Hélène Thomas-Guyon. Biomarker modulation associated with marine diesel contamination in the Iceland scallop (Chlamys islandica). Environmental Science and Pollution Research. 2015; 22 (23):19292-19296.

Chicago/Turabian Style

Thomas Milinkovitch; Perrine Geraudie; Lionel Camus; Valérie Huet; Hélène Thomas-Guyon. 2015. "Biomarker modulation associated with marine diesel contamination in the Iceland scallop (Chlamys islandica)." Environmental Science and Pollution Research 22, no. 23: 19292-19296.

Journal article
Published: 01 May 2015 in Science of The Total Environment
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The aim of this study was to determine 1) the relevance of using the Pacific oyster Crassostrea gigas as a sentinel organism, at a juvenile stage, for polycyclic aromatic hydrocarbon (PAH) and persistent organic pollutant (polychlorobiphenyl, PCB, polybromodimethylether, PBDE, and organochlorine pesticide, OCP) contamination, 2) the potential levels of chemical organic contamination in the Marennes-Oléron Bay, and their potential sources and 3) the potential influence of physiological or environmental factors on contaminant body burdens in oysters. To this end, juvenile oysters purchased from an oyster hatchery were transplanted to a reference site, in Bouin, and to different transplantation sites in the Marennes-Oléron Bay, the first oyster production area in France, and in the Gironde Estuary, the biggest estuary in Occidental Europe. Transplantations were done during summer and winter. Whole oyster soft tissues from each site were analysed for PAHs, PCBs, PBDEs and OCPs. Results obtained with a transplantation period of 3months suggest that C. gigas, at the juvenile stage, is a relevant sentinel organism for short-term assessment of contamination for these contaminants. In addition, no significant effects of physiological factors on contaminant body burdens were observed. Principal component analysis revealed two distinct groups of contaminants (PAHs and OPCs, and PCBs and PBDEs) and three groups of sites: 1) the reference site, 2) Les Palles (LP) and Boyard (BOY) in winter and 3) all the other sites. The group of LP and BOY was clearly defined by the levels of PAHs and OCPs, suggesting higher levels of contamination of these chemical compounds on these sites, potentially due to local contamination sources. In addition, no relevant effects of physiological or environmental factors on contaminant body burdens were observed. Results suggest also a predominance of contaminants related to agricultural activities along the Marennes-Oléron Bay, and therefore, further studies on the presence of pesticides in this region should be considered.

ACS Style

A. Luna-Acosta; H. Budzinski; K. Le Menach; Helene Thomas-Guyon; Paco Bustamante. Persistent organic pollutants in a marine bivalve on the Marennes-Oléron Bay and the Gironde Estuary (French Atlantic Coast)—Part 1: Bioaccumulation. Science of The Total Environment 2015, 514, 500 -510.

AMA Style

A. Luna-Acosta, H. Budzinski, K. Le Menach, Helene Thomas-Guyon, Paco Bustamante. Persistent organic pollutants in a marine bivalve on the Marennes-Oléron Bay and the Gironde Estuary (French Atlantic Coast)—Part 1: Bioaccumulation. Science of The Total Environment. 2015; 514 ():500-510.

Chicago/Turabian Style

A. Luna-Acosta; H. Budzinski; K. Le Menach; Helene Thomas-Guyon; Paco Bustamante. 2015. "Persistent organic pollutants in a marine bivalve on the Marennes-Oléron Bay and the Gironde Estuary (French Atlantic Coast)—Part 1: Bioaccumulation." Science of The Total Environment 514, no. : 500-510.

Journals
Published: 22 April 2015 in Environmental Science: Processes & Impacts
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Dispersant application is used as a response technique to minimize the environmental risk of an oil spill.

ACS Style

Soumaya Elarbaoui; Marion Richard; Fehmi Boufahja; Ezzeddine Mahmoudi; Hélène Thomas-Guyon. Effect of crude oil exposure and dispersant application on meiofauna: an intertidal mesocosm experiment. Environmental Science: Processes & Impacts 2015, 17, 997 -1004.

AMA Style

Soumaya Elarbaoui, Marion Richard, Fehmi Boufahja, Ezzeddine Mahmoudi, Hélène Thomas-Guyon. Effect of crude oil exposure and dispersant application on meiofauna: an intertidal mesocosm experiment. Environmental Science: Processes & Impacts. 2015; 17 (5):997-1004.

Chicago/Turabian Style

Soumaya Elarbaoui; Marion Richard; Fehmi Boufahja; Ezzeddine Mahmoudi; Hélène Thomas-Guyon. 2015. "Effect of crude oil exposure and dispersant application on meiofauna: an intertidal mesocosm experiment." Environmental Science: Processes & Impacts 17, no. 5: 997-1004.

Journal article
Published: 01 April 2015 in Aquatic Toxicology
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Although the variegated scallop Mimachlamys varia seems to be a suitable sentinel species for contaminant monitoring, no study has identified biomarkers in this species. In order to fill this gap, this study conducted an in situ biomarker approach. M. varia were collected in contaminated and uncontaminated areas and responsiveness of oxidative stress and immunological biomarkers was evaluated in the digestive gland. In parallel, 14 trace element concentrations were evaluated in the same organ. Superoxide dismutase activity and malondialdehyde content responded efficiently to in situ contamination when a certain degree of contamination was reached. Laccase-type phenoloxidase showed a high sensitivity but saturation of the response was highlighted for the highest contaminations. Additionally, correlations were found between biomarkers and trace element concentrations. Taken together, results showed that biomarker approach conducted in the digestive gland of M. varia represents a sensitive analytical tool to highlight ecotoxicological issues in coastal marine ecosystems.

ACS Style

Thomas Milinkovitch; Paco Bustamante; Valérie Huet; Anaïs Reigner; Carine Churlaud; Hélène Thomas-Guyon. In situ evaluation of oxidative stress and immunological parameters as ecotoxicological biomarkers in a novel sentinel species (Mimachlamys varia). Aquatic Toxicology 2015, 161, 170 -175.

AMA Style

Thomas Milinkovitch, Paco Bustamante, Valérie Huet, Anaïs Reigner, Carine Churlaud, Hélène Thomas-Guyon. In situ evaluation of oxidative stress and immunological parameters as ecotoxicological biomarkers in a novel sentinel species (Mimachlamys varia). Aquatic Toxicology. 2015; 161 ():170-175.

Chicago/Turabian Style

Thomas Milinkovitch; Paco Bustamante; Valérie Huet; Anaïs Reigner; Carine Churlaud; Hélène Thomas-Guyon. 2015. "In situ evaluation of oxidative stress and immunological parameters as ecotoxicological biomarkers in a novel sentinel species (Mimachlamys varia)." Aquatic Toxicology 161, no. : 170-175.

Journal article
Published: 21 March 2014 in Environmental Science and Pollution Research
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The objective of this paper is to synthesize results from seven published research papers employing different experimental approaches to evaluate the fate of metal-based nanoparticles (Ag NPs, Au NPs, CuO NPs, CdS NPs, ZnO NPs) in the marine environment and their effects on two marine endobenthic species, the bivalve Scrobicularia plana and the ragworm Hediste diversicolor. The experiments were carried out under laboratory (microcosms) conditions or under environmentally realistic conditions in outdoor mesocosms. Based on results from these seven papers, we addressed the following research questions: (1) How did the environment into which nanoparticles were released affect their physicochemical properties?, (2) How did the route of exposure (seawater, food, sediment) influence bioaccumulation and effects?, (3) Which biomarkers were the most responsive? and (4) Which tools were the most efficient to evaluate the fate and effects of NPs in the marine environment? The obtained results showed that metal‐based NPs in general were highly agglomerated/aggregated in seawater. DGT tools could be used to estimate the bioavailability of metals released from NPs under soluble form in the aquatic environment. Both metal forms (nanoparticulate, soluble) were generally bioaccumulated in both species. Among biochemical tools, GST and CAT were the most sensitive revealing the enhancement of anti-oxidant defenses in both species exposed to sub-lethal concentrations of metal-based NPs. Apoptosis and genotoxicity were frequently observed.

ACS Style

Catherine Mouneyrac; Pierre-Emmanuel Buffet; Laurence Poirier; Aurore Zalouk-Vergnoux; Marielle Guibbolini; Christine Risso-De Faverney; Douglas Gilliland; Déborah Berhanu; Agnieszka Dybowska; Amélie Chatel; Hanane Perrein-Ettajni; Jin-Fen Pan; Helene Thomas-Guyon; Paul Reip; Eugenia Valsami-Jones. Fate and effects of metal-based nanoparticles in two marine invertebrates, the bivalve mollusc Scrobicularia plana and the annelid polychaete Hediste diversicolor. Environmental Science and Pollution Research 2014, 21, 7899 -7912.

AMA Style

Catherine Mouneyrac, Pierre-Emmanuel Buffet, Laurence Poirier, Aurore Zalouk-Vergnoux, Marielle Guibbolini, Christine Risso-De Faverney, Douglas Gilliland, Déborah Berhanu, Agnieszka Dybowska, Amélie Chatel, Hanane Perrein-Ettajni, Jin-Fen Pan, Helene Thomas-Guyon, Paul Reip, Eugenia Valsami-Jones. Fate and effects of metal-based nanoparticles in two marine invertebrates, the bivalve mollusc Scrobicularia plana and the annelid polychaete Hediste diversicolor. Environmental Science and Pollution Research. 2014; 21 (13):7899-7912.

Chicago/Turabian Style

Catherine Mouneyrac; Pierre-Emmanuel Buffet; Laurence Poirier; Aurore Zalouk-Vergnoux; Marielle Guibbolini; Christine Risso-De Faverney; Douglas Gilliland; Déborah Berhanu; Agnieszka Dybowska; Amélie Chatel; Hanane Perrein-Ettajni; Jin-Fen Pan; Helene Thomas-Guyon; Paul Reip; Eugenia Valsami-Jones. 2014. "Fate and effects of metal-based nanoparticles in two marine invertebrates, the bivalve mollusc Scrobicularia plana and the annelid polychaete Hediste diversicolor." Environmental Science and Pollution Research 21, no. 13: 7899-7912.

Journal article
Published: 01 February 2014 in Science of The Total Environment
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Silver nanoparticles are widely used in a range of products and processes for their antibacterial properties, electrical and thermal conductivity. The fate and effects of Ag nanoparticles were examined in two endobenthic species (Scrobicularia plana, Hediste diversicolor), under environmentally realistic conditions in outdoor mesocosms exposed to Ag at 10 μg L(-1) in nanoparticulate (Ag NPs) or soluble salt (AgNO3) forms for 21 days. Labile Ag was determined in water and sediment by using diffusive gradient in thin films. Ag levels were equivalent in contaminated Ag NPs mesocosms to those contaminated with the soluble form. Bioaccumulation of Ag was observed for both species exposed to either Ag in the nanoparticulate or ionic forms. Concerning biomarker responses, both soluble and nanoparticulate Ag forms, induced defenses against oxidative stress, detoxification, apoptosis, genotoxicity and immunomodulation. Nevertheless, DNA damages measured by the comet assay in the digestive gland of S. plana, and Phenoloxidase and lysozyme activities in S. plana and H. diversicolor, respectively, were higher in the presence of Ag NPs compared to soluble Ag suggesting a specific nano effect.

ACS Style

Pierre-Emmanuel Buffet; Aurore Zalouk-Vergnoux; Amélie Châtel; Brigitte Berthet; Isabelle Métais; Hanane Perrein-Ettajani; Laurence Poirier; Andrea Luna-Acosta; Helene Thomas-Guyon; Christine Risso-De Faverney; Marielle Guibbolini; Douglas Gilliland; Eugenia Valsami-Jones; Catherine Mouneyrac. A marine mesocosm study on the environmental fate of silver nanoparticles and toxicity effects on two endobenthic species: The ragworm Hediste diversicolor and the bivalve mollusc Scrobicularia plana. Science of The Total Environment 2014, 470-471, 1151 -1159.

AMA Style

Pierre-Emmanuel Buffet, Aurore Zalouk-Vergnoux, Amélie Châtel, Brigitte Berthet, Isabelle Métais, Hanane Perrein-Ettajani, Laurence Poirier, Andrea Luna-Acosta, Helene Thomas-Guyon, Christine Risso-De Faverney, Marielle Guibbolini, Douglas Gilliland, Eugenia Valsami-Jones, Catherine Mouneyrac. A marine mesocosm study on the environmental fate of silver nanoparticles and toxicity effects on two endobenthic species: The ragworm Hediste diversicolor and the bivalve mollusc Scrobicularia plana. Science of The Total Environment. 2014; 470-471 ():1151-1159.

Chicago/Turabian Style

Pierre-Emmanuel Buffet; Aurore Zalouk-Vergnoux; Amélie Châtel; Brigitte Berthet; Isabelle Métais; Hanane Perrein-Ettajani; Laurence Poirier; Andrea Luna-Acosta; Helene Thomas-Guyon; Christine Risso-De Faverney; Marielle Guibbolini; Douglas Gilliland; Eugenia Valsami-Jones; Catherine Mouneyrac. 2014. "A marine mesocosm study on the environmental fate of silver nanoparticles and toxicity effects on two endobenthic species: The ragworm Hediste diversicolor and the bivalve mollusc Scrobicularia plana." Science of The Total Environment 470-471, no. : 1151-1159.

Journal article
Published: 01 February 2013 in Ecotoxicology and Environmental Safety
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International audienceDispersant use is a controversial oil spill response technique in coastal areas. Using an experimental approach, this study evaluated the toxicity of dispersant use upon juveniles of golden grey mullet (Liza aurata). Fish were exposed for 48 hours to either dispersant only, chemically dispersed oil, mechanically dispersed oil, the water-soluble fraction of oil or to control conditions. Following exposure and a depuration period, biomarkers were assessed in fish hearts, namely the total glutathione content and the activity of four enzymes (glutathione S-transferase, superoxide dismutase, catalase and glutathione peroxides). Comparing biomarker responses between the different treatments, this study revealed that 48 hours exposure to dispersed oil (whether mechanically or chemically dispersed) resulted in a toxicity that was still detectable after a 14 days depuration period. Comparing biomarkers responses after an exposure to chemically and mechanically dispersed oil, this study suggests that chemical dispersion of the oil slick would not be more toxic than its natural dispersion under certain turbulent meteorological conditions (e.g. waves). Furthermore, the results indicated that the heart could be a target organ of interest in further studies investigating the toxicity of hydrocarbons. This study, which has been integrated into the DISCOBIOL project (Dispersant et techniques de lutte en milieu côtier: effets biologiques et apport à la réglementation), presents information of interest when attempting to provide a framework for dispersant applications in coastal areas

ACS Style

Thomas Milinkovitch; Nathalie Imbert; Wilfried Sanchez; Stéphane Le Floch; Hélène Thomas-Guyon. Toxicological effects of crude oil and oil dispersant: Biomarkers in the heart of the juvenile golden grey mullet (Liza aurata). Ecotoxicology and Environmental Safety 2013, 88, 1 -8.

AMA Style

Thomas Milinkovitch, Nathalie Imbert, Wilfried Sanchez, Stéphane Le Floch, Hélène Thomas-Guyon. Toxicological effects of crude oil and oil dispersant: Biomarkers in the heart of the juvenile golden grey mullet (Liza aurata). Ecotoxicology and Environmental Safety. 2013; 88 ():1-8.

Chicago/Turabian Style

Thomas Milinkovitch; Nathalie Imbert; Wilfried Sanchez; Stéphane Le Floch; Hélène Thomas-Guyon. 2013. "Toxicological effects of crude oil and oil dispersant: Biomarkers in the heart of the juvenile golden grey mullet (Liza aurata)." Ecotoxicology and Environmental Safety 88, no. : 1-8.

Journal article
Published: 10 January 2013 in Environmental Science & Technology
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The fate and effects of CuO nanoparticles (CuO NPs) were examined in endobenthic species (Scrobicularia plana , Hediste diversicolor), under environmentally realistic conditions in outdoor mesocosms (exposure to Cu at 10 μg L(-1) in particulate (CuO NPs) or soluble salt (CuNO(3)) forms) for 21 days. Labile Cu was determined in water and sediment by using diffusive gradient in thin films. No labile Cu being detected from CuO NPs; the observed effects in invertebrates exposed to CuO NPs were mainly attributed to the toxicity of nanoparticulate rather than dissolved Cu toxicity. Bioaccumulation of CuO NPs was observed in both species. Biomarkers were examined at different levels of biological organization: biochemical markers of defense and damage, biomarkers of genotoxicity (comet assay), and behavioral biomarkers (feeding and burrowing). Behavioral biomarkers, antioxidant defenses (catalase, glutathion S-transferase, metallothionein), and genotoxicity are the most sensitive tools to highlight the effect of soluble or nanoparticulate metal forms. Concerning other biomarkers of defense (superoxide dismutase, lactate dehydrogenase, laccase) and damage (thiobarbituric acid reactive substances, acetylcholinesterase, acid phosphatase), no significant effects were detected. This experiment shows the suitability of mesocosms for studying the environmental effects of nanoparticles.

ACS Style

Pierre-Emmanuel Buffet; Marion Richard; Fanny Caupos; Aurore Vergnoux; Hanane Perrein-Ettajani; Andrea Luna-Acosta; Farida Akcha; Jean-Claude Amiard; Claude Amiard-Triquet; Marielle Guibbolini; Christine Risso-De Faverney; Hélène Thomas-Guyon; Paul Reip; Agnieska Dybowska; Deborah Berhanu; Eugenia Valsami-Jones; Catherine Mouneyrac; Agnieszka Dybowska. A Mesocosm Study of Fate and Effects of CuO Nanoparticles on Endobenthic Species (Scrobicularia plana, Hediste diversicolor). Environmental Science & Technology 2013, 47, 1 .

AMA Style

Pierre-Emmanuel Buffet, Marion Richard, Fanny Caupos, Aurore Vergnoux, Hanane Perrein-Ettajani, Andrea Luna-Acosta, Farida Akcha, Jean-Claude Amiard, Claude Amiard-Triquet, Marielle Guibbolini, Christine Risso-De Faverney, Hélène Thomas-Guyon, Paul Reip, Agnieska Dybowska, Deborah Berhanu, Eugenia Valsami-Jones, Catherine Mouneyrac, Agnieszka Dybowska. A Mesocosm Study of Fate and Effects of CuO Nanoparticles on Endobenthic Species (Scrobicularia plana, Hediste diversicolor). Environmental Science & Technology. 2013; 47 (3):1.

Chicago/Turabian Style

Pierre-Emmanuel Buffet; Marion Richard; Fanny Caupos; Aurore Vergnoux; Hanane Perrein-Ettajani; Andrea Luna-Acosta; Farida Akcha; Jean-Claude Amiard; Claude Amiard-Triquet; Marielle Guibbolini; Christine Risso-De Faverney; Hélène Thomas-Guyon; Paul Reip; Agnieska Dybowska; Deborah Berhanu; Eugenia Valsami-Jones; Catherine Mouneyrac; Agnieszka Dybowska. 2013. "A Mesocosm Study of Fate and Effects of CuO Nanoparticles on Endobenthic Species (Scrobicularia plana, Hediste diversicolor)." Environmental Science & Technology 47, no. 3: 1.

Journal article
Published: 28 July 2012 in Fish Physiology and Biochemistry
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Dispersant use is a controversial technique used to respond to oil spills in nearshore areas. In order to assess the toxicity of this technique, this study evaluated the cardiac toxicological effects on juvenile golden grey mullets Liza aurata exposed for 48 h to either dispersant alone, chemically dispersed oil, mechanically dispersed oil, the water-soluble fraction of oil or a control condition. Following exposure, the positive inotropic effects of adrenaline were assessed in order to evaluate a potential impairment on the cardiac performance. The results revealed an impairment of the positive inotropic effects of adrenaline for all the contaminants (single dispersant, dispersed and undispersed oil, water-soluble fraction of oil). This suggests that: (1) cardiac performance is a valuable parameter to study the physiopathological effects of dispersed oil; (2) dispersant application is likely to impair cardiac performance.

ACS Style

Thomas Milinkovitch; Helene Thomas-Guyon; Christel Lefrançois; Nathalie Imbert. Dispersant use as a response to oil spills: toxicological effects on fish cardiac performance. Fish Physiology and Biochemistry 2012, 39, 257 -262.

AMA Style

Thomas Milinkovitch, Helene Thomas-Guyon, Christel Lefrançois, Nathalie Imbert. Dispersant use as a response to oil spills: toxicological effects on fish cardiac performance. Fish Physiology and Biochemistry. 2012; 39 (2):257-262.

Chicago/Turabian Style

Thomas Milinkovitch; Helene Thomas-Guyon; Christel Lefrançois; Nathalie Imbert. 2012. "Dispersant use as a response to oil spills: toxicological effects on fish cardiac performance." Fish Physiology and Biochemistry 39, no. 2: 257-262.

Journal article
Published: 30 June 2012 in Chemosphere
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In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 μg L−1, the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides diuron and isoproturon, and the pharmaceutical ibuprofen, catecholase-type PO activity in the plasma and the percentage of phagocytosis in the haemocytes were reduced after 6 h of exposure. Our results showed that early effects on molecular, biochemical and cellular parameters can be detected in the presence of diuron alone or within a mixture, giving an insight of its potential effect in situations that can be found in natural environments, i.e. relatively high concentrations for short periods of time.

ACS Style

A. Luna-Acosta; T. Renault; Helene Thomas-Guyon; N. Faury; D. Saulnier; H. Budzinski; K. Le Menach; Patrick Pardon; Ingrid Arnaudin; Paco Bustamante. Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat. Chemosphere 2012, 87, 1335 -1340.

AMA Style

A. Luna-Acosta, T. Renault, Helene Thomas-Guyon, N. Faury, D. Saulnier, H. Budzinski, K. Le Menach, Patrick Pardon, Ingrid Arnaudin, Paco Bustamante. Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat. Chemosphere. 2012; 87 (11):1335-1340.

Chicago/Turabian Style

A. Luna-Acosta; T. Renault; Helene Thomas-Guyon; N. Faury; D. Saulnier; H. Budzinski; K. Le Menach; Patrick Pardon; Ingrid Arnaudin; Paco Bustamante. 2012. "Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat." Chemosphere 87, no. 11: 1335-1340.