The blood–brain barrier (BBB) is a highly selective cellular monolayer unique to the microvasculature of the central nervous system (CNS), and it mediates the communication of the CNS with the rest of the body by regulating the passage of molecules into the CNS microenvironment. Limitation of passage of substances through the BBB is mainly due to tight junctions (TJ) and adherens junctions (AJ) between brain microvascular endothelial cells. The importance of actin filaments and microtubules in establishing and maintaining TJs and AJs has been indicated; however, recent studies have shown that intermediate filaments are also important in the formation and function of cell–cell junctions. The most common intermediate filament protein in endothelial cells is vimentin. Vimentin plays a role in blood–brain barrier permeability in both cell–cell and cell–matrix interactions by affecting the actin and microtubule reorganization and by binding directly to VE-cadherin or integrin proteins. The BBB permeability increases due to the formation of stress fibers and the disruption of VE–cadherin interactions between two neighboring cells in various diseases, disrupting the fiber network of intermediate filament vimentin in different ways. Intermediate filaments may be long ignored key targets in regulation of BBB permeability in health and disease.

Cyclodextrins (CDs) are increasingly drawing attention as potential therapeutic tools in the treatment of cholesterol-associated diseases. However, bioavailability and delivery of CDs in the monomeric form remain still challenging. CD-based macromolecular systems seem to display promising capacity in overcoming some of these limitations. Therefore, smart, stimuli-responsive nano-systems are currently being investigated in order to provide improved CD-releasing agents. Herein we present a novel class of CD-based polymersome microparticles (CD-PMs) designed for potential therapeutic use. A new synthetic route to obtain a CD-appended, pH-sensitive polymer that self-assembles into stable polymersome microparticles is reported. Through an easy-to-use approach, a benzoic imine bond is incorporated into a poly(ε-caprolactone) backbone and employed as a building block in the construction of the nanoarchitecture. The CD-PMs show cellular uptake representing a promising potential therapeutic tool in the treatment of cholesterol-associated conditions such as neurodegenerative disease.

The impact of different types of extremely low-frequency electromagnetic fields (ELF-EMF) on the growth of Staphylococcus aureus and Escherichia coli O157:H7 was investigated. The cultures of bacteria in broth media were exposed to sinusoidal homogenous ELF-EMF with 2 and 4 mT magnetic intensities. Each intensity for each bacteria was combined with three different frequencies (20, 40 and 50 Hz), and four different exposure times (1, 2, 4 and 6 h). A cell suspension of each experiment was diluted for the appropriate range and inoculated to Mueller-Hinton Agar (MHA) plates after exposure to ELF-EMF. The number of colony forming units (CFU) of both strains was obtained after incubation at 37 °C for 24 h. Data were statistically evaluated by one-way analysis of variance (ANOVA), statistical significance was described at p < 0.05 and data were compared with their non-exposed controls. Magnetic intensity, frequency and exposure time of ELF-EMFs changed the characteristic responses for both microorganisms. Samples exposed to ELF-EMF showed a statistically significant decrease compared to their controls in colony forming capability, especially at long exposure times. An exposure to 4 mT-20 Hz ELF-EMF of 6 h produced maximum inhibition of CFU compared to their controls for both microorganisms (95.2% for S. aureus and 85% for E. coli).