This paper presents an assessment of three potential applications of municipal treated wastewater in a semiarid region of northern Mexico. The potential applications considered are agriculture, industry, and watering urban green areas. The results indicate that in the best scenario, the maximal application of treated wastewater is 150 L/s for industrial activities. Besides industrial applications, this scenario would allow farmers and urban green areas to receive 980 L/s and 70 L/s of treated wastewater respectively. Other issues and alternatives are also identified. With the implementation of this scenario, it is possible to improve the environmental, and even the socioeconomic conditions, of the study region.

This study focuses on identifying relationships between the content of heavy metals in water and the resistance patterns of different bacteria. Samples from watercourses in one of the most important mining areas in Mexico were collected. Seventy-one bacteria were isolated, and their resistance to Cr, Zn, Cu, Ag, Hg, and Co was studied. The Minimum Inhibitory Concentration range was determined, and a Multiple Metal Resistant index was calculated. After that, 11 isolated bacteria were chosen to estimate kinetic parameters. The obtained results show differences in the behavior of the studied bacteria concerning the presence of heavy metals in the media: (1) without effect, (2) inhibited growth; and (3) considerable inhibited growth. Finally, a Performance Index was proposed to select adequate bacteria for heavy metals removal; five bacteria were selected. Among them, Pseudomonas koreensis was identified as a good candidate for a future biosorption system since these bacteria can stimulate growth in the presence of all the metals tested.

Adequate treatment of the organic fraction of municipal solid waste (OFMSW) in co-digestion with sewage sludge (SS) through dark fermentation (DF) technologies has been widely studied and recognized. However, there is little experience with a high-solids approach, where practical and scalable conditions are established to lay the groundwork for further development of feasible industrial-scale projects. In this study, the biochemical hydrogen potential of OFMSW using a 7 L batch reactor at mesophilic conditions was evaluated. Parameters such as pH, redox potential, temperature, alkalinity, total solids, and substrate/inoculum ratio were adjusted and monitored. Biogas composition was analyzed by gas chromatography. The microbial characterization of SS and post-reaction percolate liquids was determined through metagenomics analyses. Results show a biohydrogen yield of 38.4 NmLH₂/gVS OFMSW, which forms ~60% of the produced biogas. Aeration was proven to be an efficient inoculum pretreatment method, mainly to decrease the levels of methanogenic archaea and metabolic competition, and at the same time maintain the required total solid (TS) contents for high-solids conditions. The microbial community analysis reveals that biohydrogen production was carried out by specific anaerobic and aerobic bacteria, predominantly dominated by the phylum Firmicutes, including the genus Bacillus (44.63% of the total microbial community), Clostridium, Romboutsia, and the phylum Proteobacteria, with the genus Proteus.